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The map label for this gene is dapE [H]

Identifier: 16272076

GI number: 16272076

Start: 108685

End: 109818

Strand: Reverse

Name: dapE [H]

Synonym: HI0102

Alternate gene names: 16272076

Gene position: 109818-108685 (Counterclockwise)

Preceding gene: 16272077

Following gene: 16272069

Centisome position: 6.0

GC content: 41.09

Gene sequence:

>1134_bases
ATGAAAGAAAAAGTGGTTTCGTTGGCACAAGATTTAATTCGTCGCCCGTCTATTAGCCCAAATGACGAAGGCTGTCAGCA
AATTATTGCCGAACGTTTAGAAAAATTAGGTTTTCAAATTGAATGGATGCCTTTTAATGACACGTTGAATTTGTGGGCAA
AACACGGAACGAGCGAGCCAGTTATTGCTTTTGCAGGTCATACGGATGTTGTGCCTACTGGCGATGAAAATCAATGGTCG
TCTCCGCCGTTTTCTGCGGAGATTATTGATGGCATGCTTTATGGGCGTGGTGCGGCGGATATGAAAGGCTCCCTTGCAGC
AATGATTGTGGCGGCAGAAGAATACGTAAAAGCAAATCCTAATCATAAAGGCACCATTGCATTATTGATTACCTCTGATG
AAGAGGCTACCGCAAAAGATGGAACTATACACGTGGTTGAGACGTTAATGGCGCGTGATGAAAAAATTACTTATTGTATG
GTTGGCGAGCCATCGAGTGCTAAAAACTTAGGAGATGTTGTCAAAAATGGTCGCCGCGGCTCAATTACAGGAAATCTCTA
TATTCAAGGTATTCAAGGACACGTAGCCTATCCGCATTTAGCTGAAAACCCAATCCATAAAGCAGCGCTATTTTTGCAAG
AATTAACGACTTACCAATGGGATAAAGGCAATGAATTTTTCCCGCCAACAAGCCTACAAATTGCTAACATTCACGCGGGT
ACGGGAAGTAATAATGTAATTCCTGCTGAGTTATATATTCAGTTCAATTTGCGTTATTGCACAGAAGTCACGGATGAAAT
CATTAAGCAAAAAGTGGCTGAAATGCTAGAAAAACACAATTTGAAATATCGTATTGAATGGAATTTATCAGGCAAACCCT
TTTTAACAAAACCAGGTAAATTATTAGATTCGATAACCTCCGCTATTGAGGAAACCATAGGCATAACGCCAAAGGCTGAA
ACAGGTGGCGGCACGTCAGACGGTCGTTTTATTGCATTGATGGGCGCAGAAGTAGTGGAATTTGGCCCGTTAAATTCAAC
CATTCATAAAGTCAATGAATGTGTCAGTGTAGAAGACCTTGGCAAGTGCGGTGAAATTTACCACAAAATGTTAGTGAATT
TATTGGATAGCTAA

Upstream 100 bases:

>100_bases
CCCAATTATTTTACAAGATGGAAAGGCATTAATTGGATTTAATGAAAAGGAATATCAGGCTGCTTTTGCTTAAAGTGCGG
TTATTTTCTTAGGAGTTTTT

Downstream 100 bases:

>100_bases
AATGAAATTAACCCCAGAAATGCTTACAGGAAAGTCCCGTGAGCATTTAGTCAATTTACCCACAACTCATTCATCAAATC
ATTTTTTGCAAACGCAAGCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Sequences:

>Translated_377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS
>Mature_377_residues
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEPVIAFAGHTDVVPTGDENQWS
SPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANPNHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCM
VGEPSSAKNLGDVVKNGRRGSITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGKLLDSITSAIEETIGITPKAE
TGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDLGKCGEIYHKMLVNLLDS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=61.6, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI1790395, Length=267, Percent_Identity=30.3370786516854, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789236, Length=401, Percent_Identity=23.1920199501247, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17551016, Length=217, Percent_Identity=25.8064516129032, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 41354; Mature: 41354

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCC
VIAFAGHTDVVPTGDENQWSSPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANP
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGK
CCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
LLDSITSAIEETIGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEKVVSLAQDLIRRPSISPNDEGCQQIIAERLEKLGFQIEWMPFNDTLNLWAKHGTSEP
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCC
VIAFAGHTDVVPTGDENQWSSPPFSAEIIDGMLYGRGAADMKGSLAAMIVAAEEYVKANP
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHCCC
NHKGTIALLITSDEEATAKDGTIHVVETLMARDEKITYCMVGEPSSAKNLGDVVKNGRRG
CCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCC
SITGNLYIQGIQGHVAYPHLAENPIHKAALFLQELTTYQWDKGNEFFPPTSLQIANIHAG
CEEEEEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECC
TGSNNVIPAELYIQFNLRYCTEVTDEIIKQKVAEMLEKHNLKYRIEWNLSGKPFLTKPGK
CCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHH
LLDSITSAIEETIGITPKAETGGGTSDGRFIALMGAEVVEFGPLNSTIHKVNECVSVEDL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHCCHHHH
GKCGEIYHKMLVNLLDS
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA