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The map label for this gene is lipA [H]

Identifier: 16272001

GI number: 16272001

Start: 28239

End: 29201

Strand: Reverse

Name: lipA [H]

Synonym: HI0026

Alternate gene names: 16272001

Gene position: 29201-28239 (Counterclockwise)

Preceding gene: 16272002

Following gene: 16272000

Centisome position: 1.6

GC content: 39.98

Gene sequence:

>963_bases
ATGTCTACGCCTTTCAAAATGGAACGCGGTGTAAAATATCGCGATGCGGCAAAAACCTCAATTATTCCCGTAAAAAATAT
CGATCCTAATCAAGATTTATTAAAAAAACCAGAATGGATGAAAATTAAACTTCCAGCAAGTTCAGCTAAAATTGAAAGCA
TTAAAAATGGAATGCGCCGTCACGGATTACATTCTGTTTGTGAGGAAGCATCTTGTCCAAATTTACACGAATGTTTTAAT
CATGGCACAGCCACTTTTATGATTTTAGGTGCAATTTGTACGCGCCGTTGCCCATTCTGTGATGTGGCTCACGGTAAACC
ATTACCGCCAGATCCTGAAGAGCCACAAAAATTGGCAGAAACGATTCAAGATATGAAACTTAAATATGTGGTAATCACAT
CGGTGGATCGTGATGACTTGCCTGATCGTGGTGCTGGTCATTTTTCTGAATGTGTAAAAGCTGTGCGCGAGCTTAATCCT
AACATTAAGATTGAAATTTTAGTGCCTGATTTTCGTGGTCGCATTACACAGGCTTTAGAGAAATTAAAAGATAATCCGCC
AGATGTCTTTAACCATAATTTAGAAAACGTACCGCGCTTATATAAAGAAATTCGTCCAGGTGCGGATTACGAATGGTCAC
TTAAATTATTGCGTGAATTCAAAGAAATATTCCCAAATATCCCAACCAAATCTGGTTTAATGGTTGGGTTAGGCGAAACC
AACGAGGAAATTTTGCAAGTGATGCAAGATTTACGTGATAACGGTGTGACTATGCTCACGTTGGGTCAATATCTTCAACC
TAGCCGCCATCATTTGCCTGTTGCCCGTTATGTGCCGCCAACTGAATTTGATGAATTCCGTGATAAAGCCAATGAAATGG
GCTTTGAACATGCGGCTTGTGGGCCATTTGTTCGTTCTTCTTATCACGCTGATTTACAAGCCAGTGGCGGATTAGTGAAA
TAA

Upstream 100 bases:

>100_bases
TTAAACACTTCGCCAATCTTTTAGGCTATAATATAACAACTTTATAACATTTTATAAAAACTAAATGCCCAATTTGGGCA
TTTTTATTTAGGAAAATACT

Downstream 100 bases:

>100_bases
TTTAAAATTCCGCTAAATTAAACCGCACTTTTTGCTTGCAACAAAAGTGCGGTTTATTTTTACAACTCAGAATAACCGCC
AATATTGCCTATAAATTATT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNP
NIKIEILVPDFRGRITQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYEWSLKLLREFKEIFPNIPTKSGLMVGLGET
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK

Sequences:

>Translated_320_residues
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFN
HGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNP
NIKIEILVPDFRGRITQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYEWSLKLLREFKEIFPNIPTKSGLMVGLGET
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK
>Mature_319_residues
STPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRRHGLHSVCEEASCPNLHECFNH
GTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAETIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPN
IKIEILVPDFRGRITQALEKLKDNPPDVFNHNLENVPRLYKEIRPGADYEWSLKLLREFKEIFPNIPTKSGLMVGLGETN
EEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAACGPFVRSSYHADLQASGGLVK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=284, Percent_Identity=44.3661971830986, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=44.3548387096774, Blast_Score=218, Evalue=7e-57,
Organism=Escherichia coli, GI1786846, Length=321, Percent_Identity=74.1433021806854, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=41.6083916083916, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=303, Percent_Identity=41.5841584158416, Blast_Score=241, Evalue=9e-65,
Organism=Drosophila melanogaster, GI221513272, Length=284, Percent_Identity=45.4225352112676, Blast_Score=247, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36197; Mature: 36066

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRR
CCCCCCHHCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPNIKIEILVPDFRGRITQALE
HHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYKEIRPGADYEWSLKLLREFKEIFPNIPTKSGLMVGLGET
HHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAAC
HHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQASGGLVK
CHHHHHCCCCCHHCCCCCCC
>Mature Secondary Structure 
STPFKMERGVKYRDAAKTSIIPVKNIDPNQDLLKKPEWMKIKLPASSAKIESIKNGMRR
CCCCCHHCCCCHHHHCCCCEEECCCCCCCHHHHCCCCCEEEECCCCHHHHHHHHHHHHH
HGLHSVCEEASCPNLHECFNHGTATFMILGAICTRRCPFCDVAHGKPLPPDPEEPQKLAE
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
TIQDMKLKYVVITSVDRDDLPDRGAGHFSECVKAVRELNPNIKIEILVPDFRGRITQALE
HHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHH
KLKDNPPDVFNHNLENVPRLYKEIRPGADYEWSLKLLREFKEIFPNIPTKSGLMVGLGET
HHHCCCCHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCC
NEEILQVMQDLRDNGVTMLTLGQYLQPSRHHLPVARYVPPTEFDEFRDKANEMGFEHAAC
HHHHHHHHHHHHHCCEEEEECHHHHCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCCHHHC
GPFVRSSYHADLQASGGLVK
CHHHHHCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA