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The map label for this gene is lepB

Identifier: 16271990

GI number: 16271990

Start: 15013

End: 16062

Strand: Reverse

Name: lepB

Synonym: HI0015

Alternate gene names: 16271990

Gene position: 16062-15013 (Counterclockwise)

Preceding gene: 16271991

Following gene: 16271989

Centisome position: 0.88

GC content: 38.95

Gene sequence:

>1050_bases
ATGTCAAATTTATTTTTTGTGATTTTATTGGCTGTCGGCTTTGGTGTGTGGAAAGTTTTAGATTATTTTCAGTTACCAAA
TACTTTTAGTATTTTGTTACTAATTTTGACCGCACTTTCTGGCGTATTATGGTGTTATCATCGTTTTGTGGTGCTGCCAA
AACGTCATCGTCAAGTGGCACGTGCAGAACAACGTTCTGGTAAAACCTTAAGTGAGGAAGAAAAAGCCAAAATTGAACCG
ATTTCTGAGGCTTCAGAATTTTTGTCTTCACTTTTTCCTGTGCTTGCAGTGGTATTTTTGGTTCGTTCTTTTTTGTTTGA
ACCGTTTCAAATTCCCTCTGGCTCAATGGAGTCCACTTTACGCGTTGGCGATTTTTTAGTTGTGAATAAATATGCTTATG
GTGTGAAAGATCCGATTTTCCAAAACACCATTATTGCAGGCGAAAAACCACAACGTGGCGATGTGATTGTGTTTAAAGCA
CCACAACAAGCGTTAATTCGTACTGGTCTTGGGGCAACACGAGCGGCTTTTGCAGAAAATTTAGCGTTAAGTTCAAAAGA
TAATATGTCTGGTGTGGATTATATTAAGCGTATTGTTGGAAAGGGCGGAGATCGCGTCATTTTTGATGTGGAACAAAAAA
CGCTAAAAGTGGTATATGGTAAAGAGGGGAAACCCTGTGAAATTGATTGCGAAACTAAGGCGTTTGAATATACACAAAAT
CCAACAAATCCTGCTTTTCCAAATGAATTAGAATTGACTGAAAAAGGCGATGTAACACATAACGTGTTAATTAGTGAGTA
TCGTCGTTATTCAGACCTTGAATTTTTCCCACAAGAGGGAATGCAAACTGCAGAATGGCTTGTGCCAGAGGGGCAGTATT
TTGTGATGGGGGATCATCGCGATCACAGCGATGACAGTCGTTTCTGGGGCTTTGTACCTGAAAAAAATATTGTGGGTAAA
GCCACTTATATTTGGATGAGCTTAGAAAAAGAAGCGAATGAATGGCCAACAGGTTTCCGTTTTGAGCGTTTCTTTACAGC
AATAAAATAA

Upstream 100 bases:

>100_bases
AAGAAGGGAAAAAACGTATGAAGTCTTTAGGTAACGTAGAAGTGCCGCAAGAGGCGTTTTTAGCAATTTTACACGTAGGA
AAAGATAAATAAGGAAAATT

Downstream 100 bases:

>100_bases
GATGAATCATTTAGATCGACTTGAGCGTAAAATCGGCTATCGTTTTAATGATATAGCGCTCTTAAAGCAGGCACTTACGC
ATCGAAGTGCGGCAACTCAA

Product: signal peptidase I

Products: NA

Alternate protein names: SPase I; Leader peptidase I

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEP
ISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIAGEKPQRGDVIVFKA
PQQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRVIFDVEQKTLKVVYGKEGKPCEIDCETKAFEYTQN
PTNPAFPNELELTEKGDVTHNVLISEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGK
ATYIWMSLEKEANEWPTGFRFERFFTAIK

Sequences:

>Translated_349_residues
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEP
ISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIAGEKPQRGDVIVFKA
PQQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRVIFDVEQKTLKVVYGKEGKPCEIDCETKAFEYTQN
PTNPAFPNELELTEKGDVTHNVLISEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGK
ATYIWMSLEKEANEWPTGFRFERFFTAIK
>Mature_348_residues
SNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVARAEQRSGKTLSEEEKAKIEPI
SEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTLRVGDFLVVNKYAYGVKDPIFQNTIIAGEKPQRGDVIVFKAP
QQALIRTGLGATRAAFAENLALSSKDNMSGVDYIKRIVGKGGDRVIFDVEQKTLKVVYGKEGKPCEIDCETKAFEYTQNP
TNPAFPNELELTEKGDVTHNVLISEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHRDHSDDSRFWGFVPEKNIVGKA
TYIWMSLEKEANEWPTGFRFERFFTAIK

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family

Homologues:

Organism=Escherichia coli, GI1788921, Length=346, Percent_Identity=40.4624277456647, Blast_Score=255, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEP_HAEIN (P44454)

Other databases:

- EMBL:   L42023
- PIR:   H64042
- RefSeq:   NP_438188.1
- ProteinModelPortal:   P44454
- SMR:   P44454
- MEROPS:   S26.001
- GeneID:   950912
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0015
- NMPDR:   fig|71421.1.peg.14
- TIGR:   HI_0015
- HOGENOM:   HBG596607
- OMA:   VAVFKYP
- ProtClustDB:   CLSK869948
- BioCyc:   HINF71421:HI_0015-MONOMER
- BRENDA:   3.4.21.89
- GO:   GO:0006508
- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR019766
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:2.170.230.10
- PRINTS:   PR00727
- TIGRFAMs:   TIGR02227

Pfam domain/function: PF00717 Peptidase_S24; PF10502 Peptidase_S26; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.89

Molecular weight: Translated: 39734; Mature: 39603

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00501 SPASE_I_1; PS00760 SPASE_I_2; PS00761 SPASE_I_3

Important sites: ACT_SITE 115-115 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

HASH(0xafa4638)-; HASH(0xb2f8000)-; HASH(0xb2fcca0)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVA
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAEQRSGKTLSEEEKAKIEPISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTL
HHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RVGDFLVVNKYAYGVKDPIFQNTIIAGEKPQRGDVIVFKAPQQALIRTGLGATRAAFAEN
HHCCEEEEECHHCCCCCCHHCCCEECCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LALSSKDNMSGVDYIKRIVGKGGDRVIFDVEQKTLKVVYGKEGKPCEIDCETKAFEYTQN
HHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCEEEECCCCCEECCCC
PTNPAFPNELELTEKGDVTHNVLISEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCC
DHSDDSRFWGFVPEKNIVGKATYIWMSLEKEANEWPTGFRFERFFTAIK
CCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCH
>Mature Secondary Structure 
SNLFFVILLAVGFGVWKVLDYFQLPNTFSILLLILTALSGVLWCYHRFVVLPKRHRQVA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RAEQRSGKTLSEEEKAKIEPISEASEFLSSLFPVLAVVFLVRSFLFEPFQIPSGSMESTL
HHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
RVGDFLVVNKYAYGVKDPIFQNTIIAGEKPQRGDVIVFKAPQQALIRTGLGATRAAFAEN
HHCCEEEEECHHCCCCCCHHCCCEECCCCCCCCCEEEEECCHHHHHHHCCCHHHHHHHHH
LALSSKDNMSGVDYIKRIVGKGGDRVIFDVEQKTLKVVYGKEGKPCEIDCETKAFEYTQN
HHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCCCEEEECCCCCEECCCC
PTNPAFPNELELTEKGDVTHNVLISEYRRYSDLEFFPQEGMQTAEWLVPEGQYFVMGDHR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCEEEEECCC
DHSDDSRFWGFVPEKNIVGKATYIWMSLEKEANEWPTGFRFERFFTAIK
CCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800