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The map label for this gene is ilvE [H]

Identifier: 99082766

GI number: 99082766

Start: 3096162

End: 3097028

Strand: Direct

Name: ilvE [H]

Synonym: TM1040_2926

Alternate gene names: 99082766

Gene position: 3096162-3097028 (Clockwise)

Preceding gene: 99082759

Following gene: 99082768

Centisome position: 96.73

GC content: 56.75

Gene sequence:

>867_bases
ATGGCTGGATATGATGATCGCGACGGTCTGATCTGGATGGATGGCGAACTGGTGAATTGGCGCGATGCCAAGGTTCACAT
CCTGACTCATGCGATGCATTACGCGTCTTCCGTTTTTGAGGGCGAGCGCGCTTATAACGGCAAGATCTTTAAAAGCCGCG
CCCATTCCGAACGACTGATTGCATCGGCAGAGGCACTGGACATGCCGATGCCCTACTCGGTCGATGACATCGAACGCGCC
AAAGAAGAAACCCTCAAAGCCTCCGGGCTGCAGGATGCGTATGTGCGTGCGGTGGTGTGGCGCGGCTCTGGCGAAGACAT
GGGGGTCGCCTCGGCGAAAAACCCTGTGAGAATGGCCGTCGCGGTATGGGGCTGGGGTGCCTATTACGGCGATGCCAAAA
TGCAGGGCGCGAAACTCGACATCGCAGAGTTCAAACGTCCCTCGCCGGAAACGATCCCCGTGCATGCGAAGGCGGCAGGC
CTCTACATGATCTGCACCATCTCCAAGCACAAGGCCGAAGCCAAGGGCTGCTCTGATGCCTTGTTCATGGATTACCGTGG
CTATGTGGCCGAAGCCACCGGCGCAAATATCTTCTTCGTAAAAGACGGCGAGGTGCATACGCCCAAACCTGATTGCTTCC
TCAACGGTATTACCCGTCAAACGGTGATCCAGATGCTCAAAGACAAAGGCATCACCGTGCATGAACGTCATATCATGCCC
GAGGAAATGGATGGGTTTGAGCAATGCTGGCTGACAGGCACCGCCGCCGAAGTGACCCCCGTCGGCGAGATCGGGCCTTA
CAAATTCGAAGTGGGTCAGCTCACACGCGAAATCGCCGAAGAATACGAGGCCCTAGTGCGCAGCTAA

Upstream 100 bases:

>100_bases
CGAGATGTACCGAGTGGCGAGTTTGGCGCAATTATGTAAGCAAAAACACCGCTTGCTGCGCAATAACCGAAAATTGAACG
GCGCTGAAAGGGAATGCGGA

Downstream 100 bases:

>100_bases
ACCGTTGATATTCTAAAATTAAAAAAGCGCGGAGCCGAAACGCCCCGCGCTTTTTTAATTCAACCACCGTGGGTCCAGTC
TGGCGCCTTCGCCTTGTTCC

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAGYDDRDGLIWMDGELVNWRDAKVHILTHAMHYASSVFEGERAYNGKIFKSRAHSERLIASAEALDMPMPYSVDDIERA
KEETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAVAVWGWGAYYGDAKMQGAKLDIAEFKRPSPETIPVHAKAAG
LYMICTISKHKAEAKGCSDALFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIQMLKDKGITVHERHIMP
EEMDGFEQCWLTGTAAEVTPVGEIGPYKFEVGQLTREIAEEYEALVRS

Sequences:

>Translated_288_residues
MAGYDDRDGLIWMDGELVNWRDAKVHILTHAMHYASSVFEGERAYNGKIFKSRAHSERLIASAEALDMPMPYSVDDIERA
KEETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAVAVWGWGAYYGDAKMQGAKLDIAEFKRPSPETIPVHAKAAG
LYMICTISKHKAEAKGCSDALFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIQMLKDKGITVHERHIMP
EEMDGFEQCWLTGTAAEVTPVGEIGPYKFEVGQLTREIAEEYEALVRS
>Mature_287_residues
AGYDDRDGLIWMDGELVNWRDAKVHILTHAMHYASSVFEGERAYNGKIFKSRAHSERLIASAEALDMPMPYSVDDIERAK
EETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAVAVWGWGAYYGDAKMQGAKLDIAEFKRPSPETIPVHAKAAGL
YMICTISKHKAEAKGCSDALFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIQMLKDKGITVHERHIMPE
EMDGFEQCWLTGTAAEVTPVGEIGPYKFEVGQLTREIAEEYEALVRS

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=285, Percent_Identity=35.0877192982456, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787338, Length=260, Percent_Identity=25, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 32007; Mature: 31876

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGYDDRDGLIWMDGELVNWRDAKVHILTHAMHYASSVFEGERAYNGKIFKSRAHSERLI
CCCCCCCCCEEEECCEEECCCCCEEEEHHHHHHHHHHHHCCCHHCCCCEEECHHHHHHHH
ASAEALDMPMPYSVDDIERAKEETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAV
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
AVWGWGAYYGDAKMQGAKLDIAEFKRPSPETIPVHAKAAGLYMICTISKHKAEAKGCSDA
EEEECCEEECCCCCCCCEEEHHHHCCCCCCEEEEEEECCCEEEEEEECCHHHHCCCCCCC
LFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIQMLKDKGITVHERHIMP
EEECCCCEEEECCCCEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHCCCEEEECCCCC
EEMDGFEQCWLTGTAAEVTPVGEIGPYKFEVGQLTREIAEEYEALVRS
HHCCCHHHHEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGYDDRDGLIWMDGELVNWRDAKVHILTHAMHYASSVFEGERAYNGKIFKSRAHSERLI
CCCCCCCCEEEECCEEECCCCCEEEEHHHHHHHHHHHHCCCHHCCCCEEECHHHHHHHH
ASAEALDMPMPYSVDDIERAKEETLKASGLQDAYVRAVVWRGSGEDMGVASAKNPVRMAV
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEE
AVWGWGAYYGDAKMQGAKLDIAEFKRPSPETIPVHAKAAGLYMICTISKHKAEAKGCSDA
EEEECCEEECCCCCCCCEEEHHHHCCCCCCEEEEEEECCCEEEEEEECCHHHHCCCCCCC
LFMDYRGYVAEATGANIFFVKDGEVHTPKPDCFLNGITRQTVIQMLKDKGITVHERHIMP
EEECCCCEEEECCCCEEEEEECCCCCCCCCCCEECCCCHHHHHHHHHHCCCEEEECCCCC
EEMDGFEQCWLTGTAAEVTPVGEIGPYKFEVGQLTREIAEEYEALVRS
HHCCCHHHHEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]