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The map label for this gene is nusA [H]

Identifier: 99082750

GI number: 99082750

Start: 3077203

End: 3078819

Strand: Direct

Name: nusA [H]

Synonym: TM1040_2910

Alternate gene names: 99082750

Gene position: 3077203-3078819 (Clockwise)

Preceding gene: 99082749

Following gene: 99082751

Centisome position: 96.13

GC content: 58.94

Gene sequence:

>1617_bases
ATGGCTATCACCTCTGCAAACCAGCTGGAGCTGTTGCAAACCGCCGAGGCCGTGGCGCGTGAGAAAATGATCGATCCCGG
TCTGGTGGTCGAAGCGATGGAAGAATCCCTCGCCCGCGCCGCCAAGAGCCGCTACGGCAGCGAGATGGACATTCGTGTCT
CCATTGACCGCAAGACCGGTAAGGCGACTTTCACTCGTGTGCGCACCGTGGTCGAAGACGAAGAGCTCGAAAACTACCAG
TCCGAGCTGACCGTCGCGCAGGCCAAGCAGTATATGGAAGACCCCAAGGTCGGCGACACCATCGTTGATGAGGTTCCCCC
GGTCGAGATGGGCCGGATCGCGGCACAATCCGCCAAGCAGGTGATCCTGCAGAAAGTGCGCGAAGCAGAGCGGGATCGTC
AGTACGAAGAGTTCAAGGATCGCAACGGCACCATCATCAATGGCGTCGTCAAGCGAGAGGAATACGGCAACGTCATCGTC
GATATCGGATCTGGCGAAGGCATTCTGCGTCGCAACGAGAAAATCGGCCGTGAGAGCTATCGCCCGAACGACCGTATTCG
CTGCTTCATCAAGGACGTACGCCGCGAACCCCGTGGCCCGCAGATCTTCCTCAGCCGCACCGCGCCGGAGTTCATGGCCG
AGCTCTTCAAGATGGAAGTGCCTGAAATCTATGACGGCATCATCGAGATCAAGGCTGTGGCCCGTGACCCCGGTTCGCGT
GCAAAGATCGCTGTTGTGTCCTATGACGGGTCGATCGATCCGGTTGGCGCCTGTGTCGGTATGCGTGGCTCCCGCGTGCA
GGCGGTCGTGAACGAACTGCAGGGCGAAAAGATCGACATCATCCCTTGGAACGAAGATCAGCCGACCTTCCTTGTGAACG
CGCTGCAGCCCGCAGAGGTCTCCAAGGTTGTCTTGGACGAAGAAGCCGGCAAGATCGAAGTGGTTGTGCCCGACGAGCAG
CTTTCTCTGGCGATTGGCCGTCGCGGTCAGAACGTACGTCTGGCGTCTCAGCTGACCAACCTCGACATCGACATCATGAC
GGAAGAGGAAGAATCCGCACGCCGTCAGAAGGAATTCGAGGCGCGCACCGCACTGTTCATGGAAACGCTCGATCTCGACG
AGTTCTTTGCACAGCTTCTGGTTTCTGAAGGCTTCACCAACCTCGAAGAGGTCGCCTATGTCGAACTCGACGAACTCTTG
GTGATCGATGGCGTCGACGAAGGCACCGCCGAAGAACTGCAGGCCCGCGCGCGCGATTATCTCGAAGCCAAGGCCAAGGC
CGCGCTCGACAACGCCCGCAGCATGGGCGTCGAGGACAGCCTTATTGACTTTGACGGCCTGACACCCCAGATGGTTGAGG
CACTGGCGAAGGATGATGTGAAATCGCTTGAAGACTTCGCAACCTGTGCGGACTGGGAGCTTGCGGGTGGCTGGACCACC
GTCAACGGCGAGCGTGTCAAGGATGAAGGGATTCTCGAGCCCTTCGATGTGAGCCTCGAAGAGGCGCAAAATCTGGTGAT
GACGGCGCGGATTATGCTCGGCTGGGTCGACCCGGCAGAACTTGAATCCGATGCTGATGATCTCGAGGAAGAAGCCGAAG
GGGAAGCGGAAGCCTGA

Upstream 100 bases:

>100_bases
AGCGGAAGGCCGCTGGTGTTCTGAACGAAGACGCCTTTGACGACATCGAGACCGAAGGGTCCGCAGAAGGGACCACAGGG
TCCGAAGAGGAGAACAAGTA

Downstream 100 bases:

>100_bases
CCTGCTTCCCAAACTCCCAGGAGATGTGAATGACCCGTGGTGGTGCAGAAAAATCCCGTAACGACGGGCCGGAACGTAAA
TGTCTCGCCACCGGCGAGAC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAITSANQLELLQTAEAVAREKMIDPGLVVEAMEESLARAAKSRYGSEMDIRVSIDRKTGKATFTRVRTVVEDEELENYQ
SELTVAQAKQYMEDPKVGDTIVDEVPPVEMGRIAAQSAKQVILQKVREAERDRQYEEFKDRNGTIINGVVKREEYGNVIV
DIGSGEGILRRNEKIGRESYRPNDRIRCFIKDVRREPRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSR
AKIAVVSYDGSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDQPTFLVNALQPAEVSKVVLDEEAGKIEVVVPDEQ
LSLAIGRRGQNVRLASQLTNLDIDIMTEEEESARRQKEFEARTALFMETLDLDEFFAQLLVSEGFTNLEEVAYVELDELL
VIDGVDEGTAEELQARARDYLEAKAKAALDNARSMGVEDSLIDFDGLTPQMVEALAKDDVKSLEDFATCADWELAGGWTT
VNGERVKDEGILEPFDVSLEEAQNLVMTARIMLGWVDPAELESDADDLEEEAEGEAEA

Sequences:

>Translated_538_residues
MAITSANQLELLQTAEAVAREKMIDPGLVVEAMEESLARAAKSRYGSEMDIRVSIDRKTGKATFTRVRTVVEDEELENYQ
SELTVAQAKQYMEDPKVGDTIVDEVPPVEMGRIAAQSAKQVILQKVREAERDRQYEEFKDRNGTIINGVVKREEYGNVIV
DIGSGEGILRRNEKIGRESYRPNDRIRCFIKDVRREPRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSR
AKIAVVSYDGSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDQPTFLVNALQPAEVSKVVLDEEAGKIEVVVPDEQ
LSLAIGRRGQNVRLASQLTNLDIDIMTEEEESARRQKEFEARTALFMETLDLDEFFAQLLVSEGFTNLEEVAYVELDELL
VIDGVDEGTAEELQARARDYLEAKAKAALDNARSMGVEDSLIDFDGLTPQMVEALAKDDVKSLEDFATCADWELAGGWTT
VNGERVKDEGILEPFDVSLEEAQNLVMTARIMLGWVDPAELESDADDLEEEAEGEAEA
>Mature_537_residues
AITSANQLELLQTAEAVAREKMIDPGLVVEAMEESLARAAKSRYGSEMDIRVSIDRKTGKATFTRVRTVVEDEELENYQS
ELTVAQAKQYMEDPKVGDTIVDEVPPVEMGRIAAQSAKQVILQKVREAERDRQYEEFKDRNGTIINGVVKREEYGNVIVD
IGSGEGILRRNEKIGRESYRPNDRIRCFIKDVRREPRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSRA
KIAVVSYDGSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDQPTFLVNALQPAEVSKVVLDEEAGKIEVVVPDEQL
SLAIGRRGQNVRLASQLTNLDIDIMTEEEESARRQKEFEARTALFMETLDLDEFFAQLLVSEGFTNLEEVAYVELDELLV
IDGVDEGTAEELQARARDYLEAKAKAALDNARSMGVEDSLIDFDGLTPQMVEALAKDDVKSLEDFATCADWELAGGWTTV
NGERVKDEGILEPFDVSLEEAQNLVMTARIMLGWVDPAELESDADDLEEEAEGEAEA

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=460, Percent_Identity=45.2173913043478, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 59964; Mature: 59833

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITSANQLELLQTAEAVAREKMIDPGLVVEAMEESLARAAKSRYGSEMDIRVSIDRKTG
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KATFTRVRTVVEDEELENYQSELTVAQAKQYMEDPKVGDTIVDEVPPVEMGRIAAQSAKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
VILQKVREAERDRQYEEFKDRNGTIINGVVKREEYGNVIVDIGSGEGILRRNEKIGRESY
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCEEEECCCCCCHHHCCHHHCCCCC
RPNDRIRCFIKDVRREPRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSR
CCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHEEEHHHCCCCCC
AKIAVVSYDGSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDQPTFLVNALQPAEV
CEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHH
SKVVLDEEAGKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDIMTEEEESARRQKEFE
HHHHEECCCCEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCEEEEECCHHHHHHHHHHH
ARTALFMETLDLDEFFAQLLVSEGFTNLEEVAYVELDELLVIDGVDEGTAEELQARARDY
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHH
LEAKAKAALDNARSMGVEDSLIDFDGLTPQMVEALAKDDVKSLEDFATCADWELAGGWTT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
VNGERVKDEGILEPFDVSLEEAQNLVMTARIMLGWVDPAELESDADDLEEEAEGEAEA
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
AITSANQLELLQTAEAVAREKMIDPGLVVEAMEESLARAAKSRYGSEMDIRVSIDRKTG
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KATFTRVRTVVEDEELENYQSELTVAQAKQYMEDPKVGDTIVDEVPPVEMGRIAAQSAKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHH
VILQKVREAERDRQYEEFKDRNGTIINGVVKREEYGNVIVDIGSGEGILRRNEKIGRESY
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHCCEEEECCCCCCHHHCCHHHCCCCC
RPNDRIRCFIKDVRREPRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSR
CCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHEEEHHHCCCCCC
AKIAVVSYDGSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNEDQPTFLVNALQPAEV
CEEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCHHH
SKVVLDEEAGKIEVVVPDEQLSLAIGRRGQNVRLASQLTNLDIDIMTEEEESARRQKEFE
HHHHEECCCCEEEEEECCHHHHHHCCCCCCCEEHHHHHCCCCEEEEECCHHHHHHHHHHH
ARTALFMETLDLDEFFAQLLVSEGFTNLEEVAYVELDELLVIDGVDEGTAEELQARARDY
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHEECCCEEEEECCCCCCHHHHHHHHHHH
LEAKAKAALDNARSMGVEDSLIDFDGLTPQMVEALAKDDVKSLEDFATCADWELAGGWTT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCEEE
VNGERVKDEGILEPFDVSLEEAQNLVMTARIMLGWVDPAELESDADDLEEEAEGEAEA
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]