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The map label for this gene is ygcA [C]

Identifier: 99082734

GI number: 99082734

Start: 3064623

End: 3065867

Strand: Direct

Name: ygcA [C]

Synonym: TM1040_2894

Alternate gene names: 99082734

Gene position: 3064623-3065867 (Clockwise)

Preceding gene: 99082733

Following gene: 99082735

Centisome position: 95.74

GC content: 60.08

Gene sequence:

>1245_bases
ATGACAGACGCAGCCAGAACCACAGCCACGATCGAACGTTTGGGACACCAGGGCGACGGAATTGCTCCTGGGCCTATCTA
TGTGCCCCGAACCCTCCCAGGAGAGCGCGTCACCGGCGTTCTTGAGGGGCAGAACATGACCGATATTCGGATCGAGGATC
CCTCCGAGCACCGGGTCCAGGCCCCGTGTCGCCATTACAAATCCTGCGGAGGCTGCCAACTTCAGCATGTGAGTGATGAG
TTTGTGGCGCAGTGGAAGGTCGAGATCGTTCGAAATGCGCTGCTTTCGCGCAAGTTAGAGACTGATTTTCGCCCGATCCA
AACCTCGCCCACGCACTCGCGCCGGCGCGCAACCCTGACTGTGAAGCGCACCAAAAAAGGCGCAATGGCAGGGTTTCACG
GACGTGCATCGGACGTCATTACGGCCATCCCCGACTGTCACCTGCTTGCACCCGAACTGATCGCCGCCCTGCCGATGGCC
GAAGATCTCGCGCTGATCGGTGCAAGCCGCAAGGCGCCGCTGTCGGTCACCATCACAACATCGCAGGCCGGACTCGATGT
TTTGGTCAAAAATGGCAAGCCGCTCGACGGTCCCCTGCGCATTCAGCTGGCGCAGGCGGTGGACCGTCACAGCCTTGCCC
GTCTCACATGGGATGACGAGCTGTTGGCGATGGAGCAGTCACCAACGCAAGCCTTTGGTGGTGCGAATGTCTGCCCGCCA
CCGGGTGCTTTTCTGCAAGCCACACCCGAAGGTGAATCGGCGCTTGTGGCTGCGGTACGCGAAGGCGTCGGTGACGCGCG
GCGTATTGTCGACCTTTTTGCCGGGGCGGGCACGTTCTCGCTCCCCTTGGCGCAAACCGCCGAGGTTCACGCGGTCGAGG
GTGAGCGCGAGATGATGCAGGCGCTGGAACTAGGCTGGCGCAAGGCGCAAGGTCTTAAGAAAGTCACGACCGAGACCCGC
GACCTGTTTCGCAATCCGCTTTTGGTGGAGGATCTACGCCAATTTGGAAAGGTCTATGATGCGGTTGTCATCGACCCGCC
CCGCGCAGGCGCAGAAGCTCAGATCGCGCAGCTGGCCAAAGCAAAACTGCCTCTGATCTCTTATGTGTCCTGCAATCCGG
TCACATTTGCGCGCGATGCCGAAGTTCTGGTTGCGGCAGGCTACAAATTGAATTGGGTGCAGGTTGTTGACCAGTTTCGT
TGGTCATCGCATACGGAGCTGGCAGCCTCATTCAGCCTGACCTGA

Upstream 100 bases:

>100_bases
TTCATTCGCACCCAAGACGCGCCAAGCGCCGCCGCACAGTAATTTGGATCCGCGCGTTTTCGGACTTTTGCCAAAGCTGC
GCGCGCGGTAAGGCAGACCC

Downstream 100 bases:

>100_bases
CCGCAAACCAAAAGGAGCGCAGTTTACATGATTGATCGATTGAAATCCGTATTGGACAGCGATGGATTCAGCCGCTTCAT
CACCGCGGTCATCCTTGTCA

Product: 23S rRNA (uracil-5-)-methyltransferase RumA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTDAARTTATIERLGHQGDGIAPGPIYVPRTLPGERVTGVLEGQNMTDIRIEDPSEHRVQAPCRHYKSCGGCQLQHVSDE
FVAQWKVEIVRNALLSRKLETDFRPIQTSPTHSRRRATLTVKRTKKGAMAGFHGRASDVITAIPDCHLLAPELIAALPMA
EDLALIGASRKAPLSVTITTSQAGLDVLVKNGKPLDGPLRIQLAQAVDRHSLARLTWDDELLAMEQSPTQAFGGANVCPP
PGAFLQATPEGESALVAAVREGVGDARRIVDLFAGAGTFSLPLAQTAEVHAVEGEREMMQALELGWRKAQGLKKVTTETR
DLFRNPLLVEDLRQFGKVYDAVVIDPPRAGAEAQIAQLAKAKLPLISYVSCNPVTFARDAEVLVAAGYKLNWVQVVDQFR
WSSHTELAASFSLT

Sequences:

>Translated_414_residues
MTDAARTTATIERLGHQGDGIAPGPIYVPRTLPGERVTGVLEGQNMTDIRIEDPSEHRVQAPCRHYKSCGGCQLQHVSDE
FVAQWKVEIVRNALLSRKLETDFRPIQTSPTHSRRRATLTVKRTKKGAMAGFHGRASDVITAIPDCHLLAPELIAALPMA
EDLALIGASRKAPLSVTITTSQAGLDVLVKNGKPLDGPLRIQLAQAVDRHSLARLTWDDELLAMEQSPTQAFGGANVCPP
PGAFLQATPEGESALVAAVREGVGDARRIVDLFAGAGTFSLPLAQTAEVHAVEGEREMMQALELGWRKAQGLKKVTTETR
DLFRNPLLVEDLRQFGKVYDAVVIDPPRAGAEAQIAQLAKAKLPLISYVSCNPVTFARDAEVLVAAGYKLNWVQVVDQFR
WSSHTELAASFSLT
>Mature_413_residues
TDAARTTATIERLGHQGDGIAPGPIYVPRTLPGERVTGVLEGQNMTDIRIEDPSEHRVQAPCRHYKSCGGCQLQHVSDEF
VAQWKVEIVRNALLSRKLETDFRPIQTSPTHSRRRATLTVKRTKKGAMAGFHGRASDVITAIPDCHLLAPELIAALPMAE
DLALIGASRKAPLSVTITTSQAGLDVLVKNGKPLDGPLRIQLAQAVDRHSLARLTWDDELLAMEQSPTQAFGGANVCPPP
GAFLQATPEGESALVAAVREGVGDARRIVDLFAGAGTFSLPLAQTAEVHAVEGEREMMQALELGWRKAQGLKKVTTETRD
LFRNPLLVEDLRQFGKVYDAVVIDPPRAGAEAQIAQLAKAKLPLISYVSCNPVTFARDAEVLVAAGYKLNWVQVVDQFRW
SSHTELAASFSLT

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Escherichia coli, GI1789148, Length=427, Percent_Identity=30.6791569086651, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 44895; Mature: 44764

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAARTTATIERLGHQGDGIAPGPIYVPRTLPGERVTGVLEGQNMTDIRIEDPSEHRVQ
CCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCCCCCHHH
APCRHYKSCGGCQLQHVSDEFVAQWKVEIVRNALLSRKLETDFRPIQTSPTHSRRRATLT
HHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VKRTKKGAMAGFHGRASDVITAIPDCHLLAPELIAALPMAEDLALIGASRKAPLSVTITT
EEECCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEE
SQAGLDVLVKNGKPLDGPLRIQLAQAVDRHSLARLTWDDELLAMEQSPTQAFGGANVCPP
CCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHEEECCCCHHEEECCCCCHHCCCCCCCCC
PGAFLQATPEGESALVAAVREGVGDARRIVDLFAGAGTFSLPLAQTAEVHAVEGEREMMQ
CCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCCCCCEEEECCHHHHHH
ALELGWRKAQGLKKVTTETRDLFRNPLLVEDLRQFGKVYDAVVIDPPRAGAEAQIAQLAK
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHH
AKLPLISYVSCNPVTFARDAEVLVAAGYKLNWVQVVDQFRWSSHTELAASFSLT
HCCCEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCHHHEEECCC
>Mature Secondary Structure 
TDAARTTATIERLGHQGDGIAPGPIYVPRTLPGERVTGVLEGQNMTDIRIEDPSEHRVQ
CCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCEEEEECCCCCCHHH
APCRHYKSCGGCQLQHVSDEFVAQWKVEIVRNALLSRKLETDFRPIQTSPTHSRRRATLT
HHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
VKRTKKGAMAGFHGRASDVITAIPDCHLLAPELIAALPMAEDLALIGASRKAPLSVTITT
EEECCCCCCCCCCCCCCHHHEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEEEE
SQAGLDVLVKNGKPLDGPLRIQLAQAVDRHSLARLTWDDELLAMEQSPTQAFGGANVCPP
CCCCCEEEEECCCCCCCCCEEHHHHHHHHHHHEEECCCCHHEEECCCCCHHCCCCCCCCC
PGAFLQATPEGESALVAAVREGVGDARRIVDLFAGAGTFSLPLAQTAEVHAVEGEREMMQ
CCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCCCCCEEEECCHHHHHH
ALELGWRKAQGLKKVTTETRDLFRNPLLVEDLRQFGKVYDAVVIDPPRAGAEAQIAQLAK
HHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHH
AKLPLISYVSCNPVTFARDAEVLVAAGYKLNWVQVVDQFRWSSHTELAASFSLT
HCCCEEEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHCCCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]