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The map label for this gene is grpE [H]

Identifier: 99082714

GI number: 99082714

Start: 3042260

End: 3042823

Strand: Direct

Name: grpE [H]

Synonym: TM1040_2874

Alternate gene names: 99082714

Gene position: 3042260-3042823 (Clockwise)

Preceding gene: 99082713

Following gene: 99082717

Centisome position: 95.04

GC content: 59.93

Gene sequence:

>564_bases
ATGGCAGAGCCCAAGAACGAAGACTTTTTGGACGATATCGAGGCGGCCGAAGCCGAGGAGCTGTCGGCTCAGACCGAGGA
ATTTGACGACGAAGCGCTGGAGCTGGACAGCCTGCGTGCGGAGCGTGACGATTATAAGGATCGCTTTATGCGGGCGCTTG
CGGACGCCGAAAACGCGCGTAAACGCGGCGACAAGGCGCGGCGCGAAGCAGAGCAATACGGCGGCTCCAAGCTGGCCCGT
GACATGCTGCCGGTCTACGACAACATGAAGCGTGCGGTTGAGGCGGCTACAGAAGAGCAAAAGGCTGTCTCAGCGGCGCT
CATCGAAGGGGTCGAGTTGACCATGCGCGCGCTTCTGGACGTGTTCCAGAAACATGGCATCCAAGTGGTTTCGCCCGAGG
TCGGAGACCGATTTGACCCGCAGGTGCATGAGGCCATGTTCGAAGCGCCGGTTCCAGGCACAAAGGCCGGTGACATTATT
CAGGTGTCAGCCGAAGGCTTCATGTTGCATGACCGCCTGCTCCGGCCTGCTCAGGTTGGTGTGTCGTCAACACCCGCATC
CTAA

Upstream 100 bases:

>100_bases
CGGCCCCACGCGCCTGAACTACGGACGGATTGTGCCGATCGTGGACTATACGGCACAACTGGTCGGGAAAATGGTATCCG
ACCGGAGCTAGAGGTGGATT

Downstream 100 bases:

>100_bases
AAGAAATGGGCCCTGACGGGCCCATGCCTCCGGCGGAGGTATTTAGAAAAAGATGAAAGGGCGGCCGTTTCGGACCGCCT
TTCTTCTTAACCCTTGCTTA

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MAEPKNEDFLDDIEAAEAEELSAQTEEFDDEALELDSLRAERDDYKDRFMRALADAENARKRGDKARREAEQYGGSKLAR
DMLPVYDNMKRAVEAATEEQKAVSAALIEGVELTMRALLDVFQKHGIQVVSPEVGDRFDPQVHEAMFEAPVPGTKAGDII
QVSAEGFMLHDRLLRPAQVGVSSTPAS

Sequences:

>Translated_187_residues
MAEPKNEDFLDDIEAAEAEELSAQTEEFDDEALELDSLRAERDDYKDRFMRALADAENARKRGDKARREAEQYGGSKLAR
DMLPVYDNMKRAVEAATEEQKAVSAALIEGVELTMRALLDVFQKHGIQVVSPEVGDRFDPQVHEAMFEAPVPGTKAGDII
QVSAEGFMLHDRLLRPAQVGVSSTPAS
>Mature_186_residues
AEPKNEDFLDDIEAAEAEELSAQTEEFDDEALELDSLRAERDDYKDRFMRALADAENARKRGDKARREAEQYGGSKLARD
MLPVYDNMKRAVEAATEEQKAVSAALIEGVELTMRALLDVFQKHGIQVVSPEVGDRFDPQVHEAMFEAPVPGTKAGDIIQ
VSAEGFMLHDRLLRPAQVGVSSTPAS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=139, Percent_Identity=40.2877697841727, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI40255109, Length=161, Percent_Identity=31.055900621118, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788967, Length=177, Percent_Identity=31.0734463276836, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552458, Length=163, Percent_Identity=38.0368098159509, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324806, Length=176, Percent_Identity=34.6590909090909, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653432, Length=154, Percent_Identity=33.1168831168831, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20724; Mature: 20593

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPKNEDFLDDIEAAEAEELSAQTEEFDDEALELDSLRAERDDYKDRFMRALADAENAR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGDKARREAEQYGGSKLARDMLPVYDNMKRAVEAATEEQKAVSAALIEGVELTMRALLD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFQKHGIQVVSPEVGDRFDPQVHEAMFEAPVPGTKAGDIIQVSAEGFMLHDRLLRPAQVG
HHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCHHHCC
VSSTPAS
CCCCCCC
>Mature Secondary Structure 
AEPKNEDFLDDIEAAEAEELSAQTEEFDDEALELDSLRAERDDYKDRFMRALADAENAR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRGDKARREAEQYGGSKLARDMLPVYDNMKRAVEAATEEQKAVSAALIEGVELTMRALLD
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFQKHGIQVVSPEVGDRFDPQVHEAMFEAPVPGTKAGDIIQVSAEGFMLHDRLLRPAQVG
HHHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCHHHCC
VSSTPAS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA