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The map label for this gene is dapF [H]

Identifier: 99082649

GI number: 99082649

Start: 2972936

End: 2973763

Strand: Direct

Name: dapF [H]

Synonym: TM1040_2809

Alternate gene names: 99082649

Gene position: 2972936-2973763 (Clockwise)

Preceding gene: 99082644

Following gene: 99082650

Centisome position: 92.88

GC content: 57.97

Gene sequence:

>828_bases
ATGTTGCCGTTCATGAAGATGCATGGGCTTGGAAATGACTTTGTCGTCATCGATGCCCGCGAGTCAGATATCGAAATCAC
CCCTGCAATGGCCAAGGGGATTGCCCACCGCCAGATGGGTCTCGGATTTGACCAACTCGCCGTCATCGCTACCGCTGACG
CGTCTACAGAAGCGGATGCGCATCTGGTGTTCTACAACGCTGACGGCTCTACTTCAGGGGCCTGCGGGAATGCGACACGC
TGTATCGCAAGGTACCTGATGGATCAGAGCAACAAGCCGTCGCTGCGGTTGACAACCGACCGGGGTGTCCTGATTGCAAC
GGACGCAGGTGAGGGGCTGACCTCGGTGAATATGGGTGCGCCGCAGCTCAGTTGGGATGAAATCCCGCTCGCGGAGGAGT
TGGAAACGCTAGAACTCCCCATCGAAGGCGCGCCAAGCGCCACTGGCATGGGCAACCCGCATTGTACGTTCTTTGTTGAG
GACGCAGAGGTGATTCCACTGGCGGAATTTGGTCCTCGTTACGAACATCATCCCCTCTATCCGGAACGCACCAATGTTCA
GATCGCCCATGTCGTCGGCCCGAACCATCTCAGGATGCGCGTCTGGGAGCGCGGTGTTGGCATTACCCTTGCATCAGGCT
CCTCCTCCTGCGCAACAGCGGTAGCAGCCGCACGCAAGGGTCTCACAGGGCGCAAGGTACAGATCGATCTGGATGGAGGG
ACGCTGTGGATCGATTGGCGCGACGATGGGGTCTGGATGACCGGACCAACAATGCATGTCGCCAATGGCAGCTTTACACG
GCAATTCCTCGAAAGCTTGTCCGAATGA

Upstream 100 bases:

>100_bases
CAGAGGGTCAACCCCTTTTTCGAAGTTTTCTGCGCTATCTCTGGATTCATTTTAACATCAGGTCTATATGCGCTTCATGA
CCTCCCCAGGATCCGATCAT

Downstream 100 bases:

>100_bases
GCAACGCGCCAAAATTCACAACGCTCGGCTGTCGGCTCAATGCCTATGAAACAGAGGCCATGAAGGAGCTCAGCCGCCAG
GCGGGCCTTGAGGGTGCCGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLPFMKMHGLGNDFVVIDARESDIEITPAMAKGIAHRQMGLGFDQLAVIATADASTEADAHLVFYNADGSTSGACGNATR
CIARYLMDQSNKPSLRLTTDRGVLIATDAGEGLTSVNMGAPQLSWDEIPLAEELETLELPIEGAPSATGMGNPHCTFFVE
DAEVIPLAEFGPRYEHHPLYPERTNVQIAHVVGPNHLRMRVWERGVGITLASGSSSCATAVAAARKGLTGRKVQIDLDGG
TLWIDWRDDGVWMTGPTMHVANGSFTRQFLESLSE

Sequences:

>Translated_275_residues
MLPFMKMHGLGNDFVVIDARESDIEITPAMAKGIAHRQMGLGFDQLAVIATADASTEADAHLVFYNADGSTSGACGNATR
CIARYLMDQSNKPSLRLTTDRGVLIATDAGEGLTSVNMGAPQLSWDEIPLAEELETLELPIEGAPSATGMGNPHCTFFVE
DAEVIPLAEFGPRYEHHPLYPERTNVQIAHVVGPNHLRMRVWERGVGITLASGSSSCATAVAAARKGLTGRKVQIDLDGG
TLWIDWRDDGVWMTGPTMHVANGSFTRQFLESLSE
>Mature_275_residues
MLPFMKMHGLGNDFVVIDARESDIEITPAMAKGIAHRQMGLGFDQLAVIATADASTEADAHLVFYNADGSTSGACGNATR
CIARYLMDQSNKPSLRLTTDRGVLIATDAGEGLTSVNMGAPQLSWDEIPLAEELETLELPIEGAPSATGMGNPHCTFFVE
DAEVIPLAEFGPRYEHHPLYPERTNVQIAHVVGPNHLRMRVWERGVGITLASGSSSCATAVAAARKGLTGRKVQIDLDGG
TLWIDWRDDGVWMTGPTMHVANGSFTRQFLESLSE

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=37.6383763837638, Blast_Score=195, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29638; Mature: 29638

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPFMKMHGLGNDFVVIDARESDIEITPAMAKGIAHRQMGLGFDQLAVIATADASTEADA
CCCCHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
HLVFYNADGSTSGACGNATRCIARYLMDQSNKPSLRLTTDRGVLIATDAGEGLTSVNMGA
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEECCCC
PQLSWDEIPLAEELETLELPIEGAPSATGMGNPHCTFFVEDAEVIPLAEFGPRYEHHPLY
CCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCC
PERTNVQIAHVVGPNHLRMRVWERGVGITLASGSSSCATAVAAARKGLTGRKVQIDLDGG
CCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCC
TLWIDWRDDGVWMTGPTMHVANGSFTRQFLESLSE
EEEEEEECCCEEEECCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MLPFMKMHGLGNDFVVIDARESDIEITPAMAKGIAHRQMGLGFDQLAVIATADASTEADA
CCCCHHHCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCE
HLVFYNADGSTSGACGNATRCIARYLMDQSNKPSLRLTTDRGVLIATDAGEGLTSVNMGA
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEECCCC
PQLSWDEIPLAEELETLELPIEGAPSATGMGNPHCTFFVEDAEVIPLAEFGPRYEHHPLY
CCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEHHCCCCCCCCCCC
PERTNVQIAHVVGPNHLRMRVWERGVGITLASGSSSCATAVAAARKGLTGRKVQIDLDGG
CCCCCEEEEEEECCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCC
TLWIDWRDDGVWMTGPTMHVANGSFTRQFLESLSE
EEEEEEECCCEEEECCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA