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The map label for this gene is ctsA [H]

Identifier: 99082568

GI number: 99082568

Start: 2871357

End: 2872124

Strand: Reverse

Name: ctsA [H]

Synonym: TM1040_2728

Alternate gene names: 99082568

Gene position: 2872124-2871357 (Counterclockwise)

Preceding gene: 99082569

Following gene: 99082567

Centisome position: 89.73

GC content: 63.15

Gene sequence:

>768_bases
ATGAGCGATGTGAGTGATTGGTGGCGCACGTCGATCATCGACATGGAACCGGGCCGTATCGAGCTTCGGGGTCACCCAAT
TCAGGATCTCATTGGCAACGTCAGCTTTGCCGAGATGATCTGGCTAATGGTGCGTGGGGACCGACCCACGGCGCCGCAGT
CGCGTTTGTTCGAGGCCGCCTTGGTCTCGGCCGTGGATCATGGCCCGCAAGCCCCCTCGATTGCCGCGGCGCGGATGGCC
GTGACCTGCGGCTTGCCCCTGAACAATGCGATGGCGACGGCGGTGAATATGCTTGGCGACGTGCACGGCGGGGCCGGGCA
GCAGGCGGTGGAGCTGTATCACCACGTTGACGCCCACGGCGGGGATCTGGCAACGGCGCTTGATGATTGGCGCGCAGAGC
ACGGCAAGATCATCCCAGGCTTTGGTCATCGGTTTCACAAACCGACCGACCCCCGTGCGCCGCGCCTGATGGCGCTGGTG
TCCGAAATCGCCGAAACGGGGGCTGTCTCGGGACGGTTTCTGCGCATTGCCGAAGCCATCGAGGCGCATATCTCGGCAGA
AAAAGGAACTGCCCTGCCAATGAACATCGATGGGGCAACGGCTGTGATCTACGCGGAGCTTGGCTTTGCCCCGCCTCTGG
CGCGGGGGCTGTTTTGCCTGTCGCGTTCGGTGGGCATTCTGTCCCACGCATGGGAGCAGCAGCAACAGGGCGGGCGCAAC
AAGGGACCGACCCCGCCCAAATACAGATGGACGTATGACCCTAACTGA

Upstream 100 bases:

>100_bases
ATCAGGCGCCACCCGTGCTTGGTGCCGACAATACCAGCATCTGGCAGGACCTCGGCCTCAGCGCAGAAGAGATCGAAACC
TTGCGACAGGAGCGCGTGAT

Downstream 100 bases:

>100_bases
CGTGTTTGGCCTTGTGGGCGCGCTTGGCGTTGCCGTGCTTGGCACATTGATTTTTGAAGCGATTGGATTTCCCTCGGCCC
CCCTGACCGGTTCAGCGGCA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSDVSDWWRTSIIDMEPGRIELRGHPIQDLIGNVSFAEMIWLMVRGDRPTAPQSRLFEAALVSAVDHGPQAPSIAAARMA
VTCGLPLNNAMATAVNMLGDVHGGAGQQAVELYHHVDAHGGDLATALDDWRAEHGKIIPGFGHRFHKPTDPRAPRLMALV
SEIAETGAVSGRFLRIAEAIEAHISAEKGTALPMNIDGATAVIYAELGFAPPLARGLFCLSRSVGILSHAWEQQQQGGRN
KGPTPPKYRWTYDPN

Sequences:

>Translated_255_residues
MSDVSDWWRTSIIDMEPGRIELRGHPIQDLIGNVSFAEMIWLMVRGDRPTAPQSRLFEAALVSAVDHGPQAPSIAAARMA
VTCGLPLNNAMATAVNMLGDVHGGAGQQAVELYHHVDAHGGDLATALDDWRAEHGKIIPGFGHRFHKPTDPRAPRLMALV
SEIAETGAVSGRFLRIAEAIEAHISAEKGTALPMNIDGATAVIYAELGFAPPLARGLFCLSRSVGILSHAWEQQQQGGRN
KGPTPPKYRWTYDPN
>Mature_254_residues
SDVSDWWRTSIIDMEPGRIELRGHPIQDLIGNVSFAEMIWLMVRGDRPTAPQSRLFEAALVSAVDHGPQAPSIAAARMAV
TCGLPLNNAMATAVNMLGDVHGGAGQQAVELYHHVDAHGGDLATALDDWRAEHGKIIPGFGHRFHKPTDPRAPRLMALVS
EIAETGAVSGRFLRIAEAIEAHISAEKGTALPMNIDGATAVIYAELGFAPPLARGLFCLSRSVGILSHAWEQQQQGGRNK
GPTPPKYRWTYDPN

Specific function: Catalyzes The Synthesis Of 2-Methylcitrate From Propionyl-CoA And Oxaloacetate. Also Catalyzes The Condensation Of Oxaloacetate With Acetyl-CoA But With A Lower Specificity. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=213, Percent_Identity=25.8215962441315, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 27549; Mature: 27417

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVSDWWRTSIIDMEPGRIELRGHPIQDLIGNVSFAEMIWLMVRGDRPTAPQSRLFEAA
CCCHHHHHHHCEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHH
LVSAVDHGPQAPSIAAARMAVTCGLPLNNAMATAVNMLGDVHGGAGQQAVELYHHVDAHG
HHHHHHCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GDLATALDDWRAEHGKIIPGFGHRFHKPTDPRAPRLMALVSEIAETGAVSGRFLRIAEAI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
EAHISAEKGTALPMNIDGATAVIYAELGFAPPLARGLFCLSRSVGILSHAWEQQQQGGRN
HHHHCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGPTPPKYRWTYDPN
CCCCCCCEEEECCCC
>Mature Secondary Structure 
SDVSDWWRTSIIDMEPGRIELRGHPIQDLIGNVSFAEMIWLMVRGDRPTAPQSRLFEAA
CCHHHHHHHCEEECCCCEEEECCCCHHHHHCCCCHHHHHHHHEECCCCCCHHHHHHHHH
LVSAVDHGPQAPSIAAARMAVTCGLPLNNAMATAVNMLGDVHGGAGQQAVELYHHVDAHG
HHHHHHCCCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
GDLATALDDWRAEHGKIIPGFGHRFHKPTDPRAPRLMALVSEIAETGAVSGRFLRIAEAI
CHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHH
EAHISAEKGTALPMNIDGATAVIYAELGFAPPLARGLFCLSRSVGILSHAWEQQQQGGRN
HHHHCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KGPTPPKYRWTYDPN
CCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1311544 [H]