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The map label for this gene is fmt

Identifier: 99082417

GI number: 99082417

Start: 2715727

End: 2716653

Strand: Reverse

Name: fmt

Synonym: TM1040_2577

Alternate gene names: 99082417

Gene position: 2716653-2715727 (Counterclockwise)

Preceding gene: 99082418

Following gene: 99082416

Centisome position: 84.87

GC content: 65.8

Gene sequence:

>927_bases
ATGCGGGTTATCTTTATGGGAACGCCGGAGTTTTCGGTGCCGGTACTGGATGCTCTTGTCGAGGCCGGGCACGAGATTGC
GGCCGTCTACTGCCAACCTCCGCGCCCCGCAGGTCGCGGCAAAAAAGACCGTCCAACCCCGGTCCACGCCAGGGCCGAGG
CTCTGGGGCTCGAGGTGCGCCATCCGGTCTCACTCAAGGGGAGCGAGGAACAACAAGCCTTTGCCGCGCTCAACGCCGAT
GTTGCCGTAGTGGTTGCCTATGGGCTGATCCTGCCGCAGGCGGTTCTGGACGCTCCGCGCGCGGGATGCCTCAACATCCA
TGCCAGCCTGCTGCCGCGCTGGCGCGGCGCTGCGCCGATCCATCGTGCGATCATGGCCGGGGACACCCACACCGGCATTT
GCATCATGCAGATGGAAGCAGGGCTTGATACTGGCCCGGTGCTGCTGCGCAAGGAGACCGAGATCGGGGGCGAGGAAACC
ACAGCGGCGCTTCATGATCGTCTCTCGCTCATGGGCGCGCGGATGATTGTGGACGCATTGGCCACGCTACCGGATCTGAA
GGCCGAGCCGCAGCCGGAGGCGGGCGTGACCTACGCCAGCAAAATCGACAAGGCCGAGGCCCAGATCGACTGGAGCGTCG
CGGCAGAGGAGGTCGACCGCAAGATTCGCGGCCTGTCCCCGTTTCCGGGCGCGTGGTTTGAGGTCGACGGCCAGCGCCTG
AAGGTGCTCGCCTCGCGTCTTGCCGAGGGCACGGGCGCAGTGGGCACAGTGCTTGATGACAAGCTGACCGTGGCATGTGG
CAGCGGCGCAGTGGAGCTCTTGCGGCTGCAACGCGCTGGCAAAGCTGCGCAGGACCGCGAGGTCTTCCTGCGCGGCTTCA
CGCTCGCCAAGGGCACAAATCTGCTGACCGCGCAGGACGCCGAATGA

Upstream 100 bases:

>100_bases
GCAGGCATATCATGTGGCGCGCAAGCGCGTCCGCTCTGGTCGTGGCGTCCATCCTGCGCTAAGTCTGCGCAGGAACGGAT
TGCGGCACAAGGACAGCGAT

Downstream 100 bases:

>100_bases
TGCTGGCTCTGTTTGGCACGGTGGTGATCGCCGGGATCATCGGCTACGCTGCAGAGCGGAGCGGTTTCACGCAAAATGGT
GTGATCCAATCGATCATCAT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRVIFMGTPEFSVPVLDALVEAGHEIAAVYCQPPRPAGRGKKDRPTPVHARAEALGLEVRHPVSLKGSEEQQAFAALNAD
VAVVVAYGLILPQAVLDAPRAGCLNIHASLLPRWRGAAPIHRAIMAGDTHTGICIMQMEAGLDTGPVLLRKETEIGGEET
TAALHDRLSLMGARMIVDALATLPDLKAEPQPEAGVTYASKIDKAEAQIDWSVAAEEVDRKIRGLSPFPGAWFEVDGQRL
KVLASRLAEGTGAVGTVLDDKLTVACGSGAVELLRLQRAGKAAQDREVFLRGFTLAKGTNLLTAQDAE

Sequences:

>Translated_308_residues
MRVIFMGTPEFSVPVLDALVEAGHEIAAVYCQPPRPAGRGKKDRPTPVHARAEALGLEVRHPVSLKGSEEQQAFAALNAD
VAVVVAYGLILPQAVLDAPRAGCLNIHASLLPRWRGAAPIHRAIMAGDTHTGICIMQMEAGLDTGPVLLRKETEIGGEET
TAALHDRLSLMGARMIVDALATLPDLKAEPQPEAGVTYASKIDKAEAQIDWSVAAEEVDRKIRGLSPFPGAWFEVDGQRL
KVLASRLAEGTGAVGTVLDDKLTVACGSGAVELLRLQRAGKAAQDREVFLRGFTLAKGTNLLTAQDAE
>Mature_308_residues
MRVIFMGTPEFSVPVLDALVEAGHEIAAVYCQPPRPAGRGKKDRPTPVHARAEALGLEVRHPVSLKGSEEQQAFAALNAD
VAVVVAYGLILPQAVLDAPRAGCLNIHASLLPRWRGAAPIHRAIMAGDTHTGICIMQMEAGLDTGPVLLRKETEIGGEET
TAALHDRLSLMGARMIVDALATLPDLKAEPQPEAGVTYASKIDKAEAQIDWSVAAEEVDRKIRGLSPFPGAWFEVDGQRL
KVLASRLAEGTGAVGTVLDDKLTVACGSGAVELLRLQRAGKAAQDREVFLRGFTLAKGTNLLTAQDAE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=255, Percent_Identity=31.7647058823529, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21614513, Length=255, Percent_Identity=32.9411764705882, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI164663775, Length=236, Percent_Identity=31.7796610169492, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=50, Blast_Score=284, Evalue=7e-78,
Organism=Escherichia coli, GI1788589, Length=283, Percent_Identity=26.5017667844523, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI133930964, Length=263, Percent_Identity=28.8973384030418, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319458, Length=278, Percent_Identity=26.978417266187, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550868, Length=304, Percent_Identity=30.9210526315789, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=34.070796460177, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585660, Length=240, Percent_Identity=28.3333333333333, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_SILST (Q1GDF7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614571.1
- ProteinModelPortal:   Q1GDF7
- SMR:   Q1GDF7
- STRING:   Q1GDF7
- GeneID:   4076708
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2577
- NMPDR:   fig|292414.1.peg.21
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q1GDF7
- ProtClustDB:   CLSK934096
- BioCyc:   SSP292414:TM1040_2577-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32726; Mature: 32726

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIFMGTPEFSVPVLDALVEAGHEIAAVYCQPPRPAGRGKKDRPTPVHARAEALGLEVR
CEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEC
HPVSLKGSEEQQAFAALNADVAVVVAYGLILPQAVLDAPRAGCLNIHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCHH
HRAIMAGDTHTGICIMQMEAGLDTGPVLLRKETEIGGEETTAALHDRLSLMGARMIVDAL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATLPDLKAEPQPEAGVTYASKIDKAEAQIDWSVAAEEVDRKIRGLSPFPGAWFEVDGQRL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCCCCCEEECCHHH
KVLASRLAEGTGAVGTVLDDKLTVACGSGAVELLRLQRAGKAAQDREVFLRGFTLAKGTN
HHHHHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHEEEEECCCC
LLTAQDAE
EEECCCCC
>Mature Secondary Structure
MRVIFMGTPEFSVPVLDALVEAGHEIAAVYCQPPRPAGRGKKDRPTPVHARAEALGLEVR
CEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHCCEEC
HPVSLKGSEEQQAFAALNADVAVVVAYGLILPQAVLDAPRAGCLNIHASLLPRWRGAAPI
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCCCCCCCHH
HRAIMAGDTHTGICIMQMEAGLDTGPVLLRKETEIGGEETTAALHDRLSLMGARMIVDAL
HHHHHCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ATLPDLKAEPQPEAGVTYASKIDKAEAQIDWSVAAEEVDRKIRGLSPFPGAWFEVDGQRL
HHCCCCCCCCCCCCCCHHHHHHHHHHHHEEHHHHHHHHHHHHHCCCCCCCCCEEECCHHH
KVLASRLAEGTGAVGTVLDDKLTVACGSGAVELLRLQRAGKAAQDREVFLRGFTLAKGTN
HHHHHHHHCCCCCEEEEECCCEEEEECCCHHHHHHHHHHCCCCCCHHHHHHEEEEECCCC
LLTAQDAE
EEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA