Click here to switch to the map view.

The map label for this gene is fixI [H]

Identifier: 99082378

GI number: 99082378

Start: 2678162

End: 2680345

Strand: Reverse

Name: fixI [H]

Synonym: TM1040_2538

Alternate gene names: 99082378

Gene position: 2680345-2678162 (Counterclockwise)

Preceding gene: 99082379

Following gene: 99082377

Centisome position: 83.74

GC content: 62.55

Gene sequence:

>2184_bases
ATGACTGCCCAGATGCACCACCAAACGGCGGTTTGTCCGGGCTGCGCGGCCGCTCCGACAGAAGCTATTGCGCAGGCCCC
GACCGAGGCGCGTCTCGCGATCTCGTTGCCAACAATCCATTGTCAGGCCTGTATTTCCGCCGTTGAGCGCGGTCTGTCGC
AAGTGCCGGGTGTACGGTCTGCGCGGGTGAACCTCACGTTGAAGCGCGCCTTGGTGGAGGCCGAACCAGAAATGCGCGCC
GCCGACTTGGTCCCGATCTGCGCGTCGCTTGGTTACGAGGCGCATGAGTTGGATCTGGCAGCCCTGGCCGCTACGGAGAC
GGATCGGGCGGGGCGTGAACTGTTGTTGCGTCTGGCAGTGGCGGGGTTTGCGTCCATGAACGTGATGCTGCTGTCGATCT
CGGTCTGGTCCGGGGCTTCCGATGCCACCCGTGACATGTTTCACTGGATTTCGGCGGCGATCACATTCCCGGCCATCGTC
TATTCTGGGCATCCCTTCTTTAAGAACGCATGGCAGGCGTTGCGGGCGCGGCGGCTCAATATGGATGTGCCGATTGTTCT
TGCGCTGCTGTTGGCGCTGCTGACCTCCCTTTGGGAGACGACACTGTCCGGGGAGCATGCCTATTTTGATGCCGCCCTGA
CGCTGACGTTCTTCTTGCTGGCGGGGCGCTATCTCGATCATCGGACACGCGCGGCGGCCCGCTCTGCCGCCGAGGAGCTT
GCAGCGCTTGAGGTGCCGCGTGCCTGGCGACTGGACAAACAGGGACAAGAGGTCGAGGTCTCGGTCGCCGATCTGACCAT
CGGGGATTTGATCCGCGTGCGACCCGGGGGGCGCATGCCCGTAGACGGAGAGATCACCGAGGGGCGCTCGGAACTTGATC
GTTCTTTGCTCACCGGGGAAACCCTTCCGGTGCTGGCAGAGCCCGGGGGCCTTGTCTCTGCAGGGGAAGTGAACCTCACG
GGGCCGCTGACGATCCGCGCCACAGCTGTTGGCGCAGAGACGTCGCTGCACCGAATGGCGGATCTGGTTGCGATCGCCGA
GTCGGGCCGGTCGCGCTACACCTCTCTGGCAGATAGCGCTGCAAAGCTCTATGCGCCGGGGGTACATATCCTCTCGGCTC
TTGCGTTTGTTGGGTGGTATCTTTATACCTGCGATCTGCGCACGGCCCTCAATATCGCCGCAGCTGTTCTGATTATCACC
TGCCCATGTGCGCTTGGTCTTGCGGTTCCTGCAGTGACCACTGCGGCATCGGGGCGGCTGTTCCGGGCCGGGCTTCTTGT
CAAACACGCCACCGCGCTTGAACGGCTGGCCGAGGTTGATACGGTAGTTTTTGATAAGACCGGAACCTTGACGGAGGGCG
CGCCGGAAGTTGTCAACCTCGATACACTCAGCCCCAAAGAACGCTCTGTTGCGCTTGCCTTGGCGTCTGGCTCAAGTCAC
CCGCTGGCCATTGCGCTGTCGCGGGCAGCGAGAGATGCAGGGGCGGAGCCAGCTGTGCTTGAAGATCTGCGCGAAGTGCC
CGGCTATGGCACCGAGGCGCGTCTGAACGGCCAGCCGGTTCGGCTTGGGCGTGCAGCATGGGTCGGTGCTCGAGGGACGC
AAGAGCAAACTGCGACCTGGCTGGATCTCGGAGAGGGGGCGCCTGTCGCAATTTGTTTCAAAGATGCCCTTCGCGCCGGT
GCCGAAGAGGTCGTGCGCGCGCTACAGGGGGCGGGCAAGGATGTTCTGCTGATATCTGGTGACACCACAGCCGCAGTGGA
GGATCTGGCGAAGCGCCTGGGCATTCAAAGCTGGGTGGCTGAGGCCCTGCCTGAGGACAAGGCCGCTCGCATTGCGGAGC
TGACCCAGACAGGGCGCAAGGTGCTGATGGTGGGTGATGGGCTCAATGATACCGCTGCATTGGCAGGTGCGCATGTGTCG
ATCTCTCCGGCTTCGGCACTGGATGCAGCACGGGTTGCCTCAGACATTGTTTTGCTCGGCAAAGATCTAGCGCCCATCGC
TGAAGCCTGCGGCGTGGCAGAGCGTGCGACCAGACGCATCCGGGAAAACTTCCAGATCGCAACACTCTATAATGTGATTG
CGGTGCCCCTTGCGATCGCGGGCCTGGCCACCCCCTTGATCGCGGCGCTGGCGATGTCCACGTCATCCATAACTGTTTCA
CTCAACGCCTTGCGTCTGCGTTAG

Upstream 100 bases:

>100_bases
GATCCTGCACGTAAAGGACTGAGATCATGCGAGATGCCTTCTTTTTCGGCGCCTGCGGGGGGGCGACTGCCCGTTTTGTG
CAGAGGCCTGAGGCTAGACG

Downstream 100 bases:

>100_bases
AAAGCCGGGAGTGCCGTCATGACTGTTTTGTCTTATCTGATCCCAATTTCCCTTATCCTCGGCGGGGCAGGGTTGATTGG
GTTCATCTATACGGTGCGCT

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MTAQMHHQTAVCPGCAAAPTEAIAQAPTEARLAISLPTIHCQACISAVERGLSQVPGVRSARVNLTLKRALVEAEPEMRA
ADLVPICASLGYEAHELDLAALAATETDRAGRELLLRLAVAGFASMNVMLLSISVWSGASDATRDMFHWISAAITFPAIV
YSGHPFFKNAWQALRARRLNMDVPIVLALLLALLTSLWETTLSGEHAYFDAALTLTFFLLAGRYLDHRTRAAARSAAEEL
AALEVPRAWRLDKQGQEVEVSVADLTIGDLIRVRPGGRMPVDGEITEGRSELDRSLLTGETLPVLAEPGGLVSAGEVNLT
GPLTIRATAVGAETSLHRMADLVAIAESGRSRYTSLADSAAKLYAPGVHILSALAFVGWYLYTCDLRTALNIAAAVLIIT
CPCALGLAVPAVTTAASGRLFRAGLLVKHATALERLAEVDTVVFDKTGTLTEGAPEVVNLDTLSPKERSVALALASGSSH
PLAIALSRAARDAGAEPAVLEDLREVPGYGTEARLNGQPVRLGRAAWVGARGTQEQTATWLDLGEGAPVAICFKDALRAG
AEEVVRALQGAGKDVLLISGDTTAAVEDLAKRLGIQSWVAEALPEDKAARIAELTQTGRKVLMVGDGLNDTAALAGAHVS
ISPASALDAARVASDIVLLGKDLAPIAEACGVAERATRRIRENFQIATLYNVIAVPLAIAGLATPLIAALAMSTSSITVS
LNALRLR

Sequences:

>Translated_727_residues
MTAQMHHQTAVCPGCAAAPTEAIAQAPTEARLAISLPTIHCQACISAVERGLSQVPGVRSARVNLTLKRALVEAEPEMRA
ADLVPICASLGYEAHELDLAALAATETDRAGRELLLRLAVAGFASMNVMLLSISVWSGASDATRDMFHWISAAITFPAIV
YSGHPFFKNAWQALRARRLNMDVPIVLALLLALLTSLWETTLSGEHAYFDAALTLTFFLLAGRYLDHRTRAAARSAAEEL
AALEVPRAWRLDKQGQEVEVSVADLTIGDLIRVRPGGRMPVDGEITEGRSELDRSLLTGETLPVLAEPGGLVSAGEVNLT
GPLTIRATAVGAETSLHRMADLVAIAESGRSRYTSLADSAAKLYAPGVHILSALAFVGWYLYTCDLRTALNIAAAVLIIT
CPCALGLAVPAVTTAASGRLFRAGLLVKHATALERLAEVDTVVFDKTGTLTEGAPEVVNLDTLSPKERSVALALASGSSH
PLAIALSRAARDAGAEPAVLEDLREVPGYGTEARLNGQPVRLGRAAWVGARGTQEQTATWLDLGEGAPVAICFKDALRAG
AEEVVRALQGAGKDVLLISGDTTAAVEDLAKRLGIQSWVAEALPEDKAARIAELTQTGRKVLMVGDGLNDTAALAGAHVS
ISPASALDAARVASDIVLLGKDLAPIAEACGVAERATRRIRENFQIATLYNVIAVPLAIAGLATPLIAALAMSTSSITVS
LNALRLR
>Mature_726_residues
TAQMHHQTAVCPGCAAAPTEAIAQAPTEARLAISLPTIHCQACISAVERGLSQVPGVRSARVNLTLKRALVEAEPEMRAA
DLVPICASLGYEAHELDLAALAATETDRAGRELLLRLAVAGFASMNVMLLSISVWSGASDATRDMFHWISAAITFPAIVY
SGHPFFKNAWQALRARRLNMDVPIVLALLLALLTSLWETTLSGEHAYFDAALTLTFFLLAGRYLDHRTRAAARSAAEELA
ALEVPRAWRLDKQGQEVEVSVADLTIGDLIRVRPGGRMPVDGEITEGRSELDRSLLTGETLPVLAEPGGLVSAGEVNLTG
PLTIRATAVGAETSLHRMADLVAIAESGRSRYTSLADSAAKLYAPGVHILSALAFVGWYLYTCDLRTALNIAAAVLIITC
PCALGLAVPAVTTAASGRLFRAGLLVKHATALERLAEVDTVVFDKTGTLTEGAPEVVNLDTLSPKERSVALALASGSSHP
LAIALSRAARDAGAEPAVLEDLREVPGYGTEARLNGQPVRLGRAAWVGARGTQEQTATWLDLGEGAPVAICFKDALRAGA
EEVVRALQGAGKDVLLISGDTTAAVEDLAKRLGIQSWVAEALPEDKAARIAELTQTGRKVLMVGDGLNDTAALAGAHVSI
SPASALDAARVASDIVLLGKDLAPIAEACGVAERATRRIRENFQIATLYNVIAVPLAIAGLATPLIAALAMSTSSITVSL
NALRLR

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=794, Percent_Identity=28.5894206549118, Blast_Score=298, Evalue=1e-80,
Organism=Homo sapiens, GI55743073, Length=498, Percent_Identity=31.3253012048193, Blast_Score=229, Evalue=9e-60,
Organism=Homo sapiens, GI115529486, Length=562, Percent_Identity=28.1138790035587, Blast_Score=206, Evalue=9e-53,
Organism=Homo sapiens, GI4502271, Length=241, Percent_Identity=24.896265560166, Blast_Score=67, Evalue=8e-11,
Organism=Escherichia coli, GI1786691, Length=738, Percent_Identity=33.6043360433604, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1789879, Length=743, Percent_Identity=28.3983849259758, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786914, Length=533, Percent_Identity=27.3921200750469, Blast_Score=120, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17556548, Length=548, Percent_Identity=27.3722627737226, Blast_Score=188, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319772, Length=645, Percent_Identity=28.5271317829457, Blast_Score=235, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6320475, Length=623, Percent_Identity=27.2873194221509, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6325221, Length=510, Percent_Identity=22.3529411764706, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321430, Length=261, Percent_Identity=25.2873563218391, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221329854, Length=563, Percent_Identity=28.9520426287744, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 76347; Mature: 76215

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00154 ATPASE_E1_E2 ; PS01047 HMA_1 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQMHHQTAVCPGCAAAPTEAIAQAPTEARLAISLPTIHCQACISAVERGLSQVPGVRS
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
ARVNLTLKRALVEAEPEMRAADLVPICASLGYEAHELDLAALAATETDRAGRELLLRLAV
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AGFASMNVMLLSISVWSGASDATRDMFHWISAAITFPAIVYSGHPFFKNAWQALRARRLN
HHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCC
MDVPIVLALLLALLTSLWETTLSGEHAYFDAALTLTFFLLAGRYLDHRTRAAARSAAEEL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALEVPRAWRLDKQGQEVEVSVADLTIGDLIRVRPGGRMPVDGEITEGRSELDRSLLTGE
HHHCCCCCCCCCCCCCEEEEEEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCC
TLPVLAEPGGLVSAGEVNLTGPLTIRATAVGAETSLHRMADLVAIAESGRSRYTSLADSA
CCEEEECCCCEEECCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AKLYAPGVHILSALAFVGWYLYTCDLRTALNIAAAVLIITCPCALGLAVPAVTTAASGRL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FRAGLLVKHATALERLAEVDTVVFDKTGTLTEGAPEVVNLDTLSPKERSVALALASGSSH
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
PLAIALSRAARDAGAEPAVLEDLREVPGYGTEARLNGQPVRLGRAAWVGARGTQEQTATW
CEEEEEHHHHHCCCCCCHHHHHHHHCCCCCCCCEECCCCEEECHHEEECCCCCHHHHHHH
LDLGEGAPVAICFKDALRAGAEEVVRALQGAGKDVLLISGDTTAAVEDLAKRLGIQSWVA
EECCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHH
EALPEDKAARIAELTQTGRKVLMVGDGLNDTAALAGAHVSISPASALDAARVASDIVLLG
HHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCEEEECCHHHHHHHHHHHHHHEEC
KDLAPIAEACGVAERATRRIRENFQIATLYNVIAVPLAIAGLATPLIAALAMSTSSITVS
CCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LNALRLR
EEEEEEC
>Mature Secondary Structure 
TAQMHHQTAVCPGCAAAPTEAIAQAPTEARLAISLPTIHCQACISAVERGLSQVPGVRS
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
ARVNLTLKRALVEAEPEMRAADLVPICASLGYEAHELDLAALAATETDRAGRELLLRLAV
EEEEHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
AGFASMNVMLLSISVWSGASDATRDMFHWISAAITFPAIVYSGHPFFKNAWQALRARRLN
HHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHCC
MDVPIVLALLLALLTSLWETTLSGEHAYFDAALTLTFFLLAGRYLDHRTRAAARSAAEEL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALEVPRAWRLDKQGQEVEVSVADLTIGDLIRVRPGGRMPVDGEITEGRSELDRSLLTGE
HHHCCCCCCCCCCCCCEEEEEEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCC
TLPVLAEPGGLVSAGEVNLTGPLTIRATAVGAETSLHRMADLVAIAESGRSRYTSLADSA
CCEEEECCCCEEECCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AKLYAPGVHILSALAFVGWYLYTCDLRTALNIAAAVLIITCPCALGLAVPAVTTAASGRL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
FRAGLLVKHATALERLAEVDTVVFDKTGTLTEGAPEVVNLDTLSPKERSVALALASGSSH
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCC
PLAIALSRAARDAGAEPAVLEDLREVPGYGTEARLNGQPVRLGRAAWVGARGTQEQTATW
CEEEEEHHHHHCCCCCCHHHHHHHHCCCCCCCCEECCCCEEECHHEEECCCCCHHHHHHH
LDLGEGAPVAICFKDALRAGAEEVVRALQGAGKDVLLISGDTTAAVEDLAKRLGIQSWVA
EECCCCCCEEEEEHHHHHCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCHHHHHH
EALPEDKAARIAELTQTGRKVLMVGDGLNDTAALAGAHVSISPASALDAARVASDIVLLG
HHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHCCCEEEECCHHHHHHHHHHHHHHEEC
KDLAPIAEACGVAERATRRIRENFQIATLYNVIAVPLAIAGLATPLIAALAMSTSSITVS
CCHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
LNALRLR
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA