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The map label for this gene is rlmH

Identifier: 99082345

GI number: 99082345

Start: 2646706

End: 2647176

Strand: Reverse

Name: rlmH

Synonym: TM1040_2505

Alternate gene names: 99082345

Gene position: 2647176-2646706 (Counterclockwise)

Preceding gene: 99082346

Following gene: 99082342

Centisome position: 82.7

GC content: 61.57

Gene sequence:

>471_bases
ATGCGCGTACATATCTGCGCGGTCGGGCGTCTGCGCGCTGGGCCGGAAAAGACGTTGATTGATGACTATCTGCAGCGGTT
TGACCGCAGCGGGCGCGCTCTGGGTCTTGGCCCGGCCAGGGTGATTGAGGTCGAAGACAAGAAAAACGCAGGGATGGGCG
CCGAAGCGGATCTGCTGCGCAAGGCACTGCCCAAAGGGGCGCTGATCTGCACCTTGGATGAGCGGGGGAAGGTCATATCC
TCGCCGGATTTTGCCGAGAAGCTTGCAGGGTGGCGCGACATGGGGCGGCAGGACCTGGCCTTTGTCATCGGTGGTGCCGA
TGGAATCGACCCCTCGTTGCGCGCCGAAGCGGATTTTTCGATTTCCTTTGGCAAGATGGTCTGGCCACACATGATGGTGC
GCCTGATGCTGGCTGAGCAGGTCTATCGCTCCGCCACGATCCTCTCGGGCAGTCCCTATCACCGGGTCTGA

Upstream 100 bases:

>100_bases
CGGGCCTCGCCTGACCCGAGCAGGTCACACGGCCCGTTCAATTTATCCTCTCGCGTGATGCATCCTGCATGCGCAGAGCT
CTCCTAACATCGGATTTTGA

Downstream 100 bases:

>100_bases
TCTGGTCAGTGCAGGTCGATCACGGTTGTTGTGGCAGCCCATCCGTCGAGATTGCAGCGTGCCTGAATTTGCACCTGTGT
GGTGCCCGCAGGAAGACGTA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRVHICAVGRLRAGPEKTLIDDYLQRFDRSGRALGLGPARVIEVEDKKNAGMGAEADLLRKALPKGALICTLDERGKVIS
SPDFAEKLAGWRDMGRQDLAFVIGGADGIDPSLRAEADFSISFGKMVWPHMMVRLMLAEQVYRSATILSGSPYHRV

Sequences:

>Translated_156_residues
MRVHICAVGRLRAGPEKTLIDDYLQRFDRSGRALGLGPARVIEVEDKKNAGMGAEADLLRKALPKGALICTLDERGKVIS
SPDFAEKLAGWRDMGRQDLAFVIGGADGIDPSLRAEADFSISFGKMVWPHMMVRLMLAEQVYRSATILSGSPYHRV
>Mature_156_residues
MRVHICAVGRLRAGPEKTLIDDYLQRFDRSGRALGLGPARVIEVEDKKNAGMGAEADLLRKALPKGALICTLDERGKVIS
SPDFAEKLAGWRDMGRQDLAFVIGGADGIDPSLRAEADFSISFGKMVWPHMMVRLMLAEQVYRSATILSGSPYHRV

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=164, Percent_Identity=32.3170731707317, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_SILST (Q1GDM9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614499.1
- ProteinModelPortal:   Q1GDM9
- SMR:   Q1GDM9
- STRING:   Q1GDM9
- GeneID:   4076507
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2505
- NMPDR:   fig|292414.1.peg.776
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- PhylomeDB:   Q1GDM9
- ProtClustDB:   PRK00103
- BioCyc:   SSP292414:TM1040_2505-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17141; Mature: 17141

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVHICAVGRLRAGPEKTLIDDYLQRFDRSGRALGLGPARVIEVEDKKNAGMGAEADLLR
CEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCCCHHHHHH
KALPKGALICTLDERGKVISSPDFAEKLAGWRDMGRQDLAFVIGGADGIDPSLRAEADFS
HHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCE
ISFGKMVWPHMMVRLMLAEQVYRSATILSGSPYHRV
EEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
>Mature Secondary Structure
MRVHICAVGRLRAGPEKTLIDDYLQRFDRSGRALGLGPARVIEVEDKKNAGMGAEADLLR
CEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCCCHHHHHH
KALPKGALICTLDERGKVISSPDFAEKLAGWRDMGRQDLAFVIGGADGIDPSLRAEADFS
HHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCE
ISFGKMVWPHMMVRLMLAEQVYRSATILSGSPYHRV
EEHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA