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The map label for this gene is nth [H]

Identifier: 99082291

GI number: 99082291

Start: 2592128

End: 2592871

Strand: Direct

Name: nth [H]

Synonym: TM1040_2451

Alternate gene names: 99082291

Gene position: 2592128-2592871 (Clockwise)

Preceding gene: 99082286

Following gene: 99082292

Centisome position: 80.98

GC content: 56.85

Gene sequence:

>744_bases
ATGGTTAGCGCGTCTTTTGCATCGCAATGCGCCGCCCGCGGCAAATGCTTTGCGTTTTTAATTTGCGCCCCCGTTCCGCA
GCGGGCAAAAGAAGCCCCGATGGCCAAGCAACTCGATTATCATACGCTCCGCGAGATCTTTACGCGGTTCCAGGACGCCG
AGGCCGAACCCAAGGGCGAACTCGACCATGTCAACGTCTACACGTTGGTGGTGGCCGTGGCTTTGTCCGCTCAGGCCACG
GATGCTGGCGTGAACAAAGCCACCAAGGATTTGTTCAAGATTGCCGATACACCCCAAAAGATGCTCGACCTCGGCGAGGA
GGGTGTGATCGAACACATCAAGACGATTGGCCTTTATCGTAACAAGGCCAAGAACGTCATCAAGCTATCGCGCATTCTCG
TCGACGAATACGGCGGCGAAGTTCCTTGTTCGCGCGCCTCACTGGAGAGCCTACCGGGGGTCGGGCGCAAGACCGCAAAT
GTGGTTTTGAACATGTGGTGGCGGTACCCGGCACAGGCAGTGGACACCCATATCTTTCGCGTCGGCAACCGGTCGGGCAT
CTGTCCGGGCAAGGATGTAAACGCGGTCGAGCGCGCAATCGAAGACAATATCCCGGTCGACTTCCAGCTGCATGCACATC
ACTGGCTGATCCTGCATGGCCGTTATCATTGCAAGGCGCGCAAACCTCTGTGTGCGACCTGCATCATCCGTGACCTCTGC
CTCTACGAGGAAAAAACACTATGA

Upstream 100 bases:

>100_bases
CACCTCGTCCTCTTGTATTGGGTCAATACTCTGGAGACGCACCTTAGCCAACGTGCCGCGCGGCGGCGACCCGGAACCTG
CGGTTTGGCCGTCCAGGCCC

Downstream 100 bases:

>100_bases
CCAAAACCTACCAACTCGTTGGCATCGGCAATGCTGTTGTCGACGTGATCTCGCAATGTGATGACAGTTTTCTGGATCAC
ATGGGGATCGAAAAAGGCAT

Product: DNA-(apurinic or apyrimidinic site) lyase / endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVSASFASQCAARGKCFAFLICAPVPQRAKEAPMAKQLDYHTLREIFTRFQDAEAEPKGELDHVNVYTLVVAVALSAQAT
DAGVNKATKDLFKIADTPQKMLDLGEEGVIEHIKTIGLYRNKAKNVIKLSRILVDEYGGEVPCSRASLESLPGVGRKTAN
VVLNMWWRYPAQAVDTHIFRVGNRSGICPGKDVNAVERAIEDNIPVDFQLHAHHWLILHGRYHCKARKPLCATCIIRDLC
LYEEKTL

Sequences:

>Translated_247_residues
MVSASFASQCAARGKCFAFLICAPVPQRAKEAPMAKQLDYHTLREIFTRFQDAEAEPKGELDHVNVYTLVVAVALSAQAT
DAGVNKATKDLFKIADTPQKMLDLGEEGVIEHIKTIGLYRNKAKNVIKLSRILVDEYGGEVPCSRASLESLPGVGRKTAN
VVLNMWWRYPAQAVDTHIFRVGNRSGICPGKDVNAVERAIEDNIPVDFQLHAHHWLILHGRYHCKARKPLCATCIIRDLC
LYEEKTL
>Mature_247_residues
MVSASFASQCAARGKCFAFLICAPVPQRAKEAPMAKQLDYHTLREIFTRFQDAEAEPKGELDHVNVYTLVVAVALSAQAT
DAGVNKATKDLFKIADTPQKMLDLGEEGVIEHIKTIGLYRNKAKNVIKLSRILVDEYGGEVPCSRASLESLPGVGRKTAN
VVLNMWWRYPAQAVDTHIFRVGNRSGICPGKDVNAVERAIEDNIPVDFQLHAHHWLILHGRYHCKARKPLCATCIIRDLC
LYEEKTL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=192, Percent_Identity=29.6875, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=57.2139303482587, Blast_Score=239, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17554540, Length=178, Percent_Identity=31.4606741573034, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=29.3785310734463, Blast_Score=87, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27513; Mature: 27513

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSASFASQCAARGKCFAFLICAPVPQRAKEAPMAKQLDYHTLREIFTRFQDAEAEPKGE
CCCCHHHHHHHHCCCEEEEEEECCCCHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
LDHVNVYTLVVAVALSAQATDAGVNKATKDLFKIADTPQKMLDLGEEGVIEHIKTIGLYR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHH
NKAKNVIKLSRILVDEYGGEVPCSRASLESLPGVGRKTANVVLNMWWRYPAQAVDTHIFR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHE
VGNRSGICPGKDVNAVERAIEDNIPVDFQLHAHHWLILHGRYHCKARKPLCATCIIRDLC
ECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEECCEECCCCCCHHHHHHHHHHH
LYEEKTL
HHHHCCC
>Mature Secondary Structure
MVSASFASQCAARGKCFAFLICAPVPQRAKEAPMAKQLDYHTLREIFTRFQDAEAEPKGE
CCCCHHHHHHHHCCCEEEEEEECCCCHHHHHCCHHHHCCHHHHHHHHHHHHCCCCCCCCC
LDHVNVYTLVVAVALSAQATDAGVNKATKDLFKIADTPQKMLDLGEEGVIEHIKTIGLYR
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHH
NKAKNVIKLSRILVDEYGGEVPCSRASLESLPGVGRKTANVVLNMWWRYPAQAVDTHIFR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHE
VGNRSGICPGKDVNAVERAIEDNIPVDFQLHAHHWLILHGRYHCKARKPLCATCIIRDLC
ECCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCEEEEEECCEECCCCCCHHHHHHHHHHH
LYEEKTL
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]