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The map label for this gene is pckA

Identifier: 99082282

GI number: 99082282

Start: 2582496

End: 2584094

Strand: Direct

Name: pckA

Synonym: TM1040_2442

Alternate gene names: 99082282

Gene position: 2582496-2584094 (Clockwise)

Preceding gene: 99082274

Following gene: 99082285

Centisome position: 80.68

GC content: 59.66

Gene sequence:

>1599_bases
ATGACATCTGGACGGGTAAACCCGAACTTCCGCCTCGAGGATCAGGGAATAGAGGGTCTGGGCAATGTCTATTATAACCT
CATGGAACCGGCCCTTGTTCAAGAAGCGCTCAGCCGTGGCGAAGGCAACCTTGGAAAAGGGGGCGCTTTCCTCGTCACCA
CCGGCAAGTTCACGGGTCGCTCCCCCAAGGACAAACATGTGGTGAAGACGGCCTCTGTTGCCGATACGATCTGGTGGGAA
AACAACGCCGAAATGAGCCCCGAGGGCTTTGACGCGCTTTATGCGGACATGGTCGAGCACATGAAAGGCCGCGACTATTT
CGTGCAGGATCTCGTCGGGGGCGCGGACCCCGTGCACGCGATCAATGTGCGCATGGTCACCGAGCTGGCGTGGCACAACC
TCTTTATCCGCCACCTGCTGCGTCGCCCCGACCGCGAAGATCTGGATGACTTCATCGCCGATTTCACCATCATCAACTGC
CCCTCTTTCCAGGCAGACCCCAATAAGCACAACTGCCGATCCGAAACCGTGATTGCGCTGAACTTCGATCGCAAGATGAT
CCTGATCGGCGGCACCGAGTATGCGGGTGAGAACAAGAAATCCGTCTTCACCCTTCTGAACTACCTGCTCCCCGAAAAGG
GCATCATGCCGATGCACTGCTCGGCCAACCATGCCAAGGGCAACCCGGTCGACACCGCCGTGTTCTTCGGCCTGTCAGGC
ACCGGCAAAACCACGCTTTCGGCAGACCCGGACCGCGTGCTGATCGGCGATGATGAGCATGGCTGGGCGTCCAACGGCAC
CTTCAACTTTGAGGGCGGCTGTTACGCGAAGACGATCAACCTCAACCCCGAGGCGGAGCCTGAGATTTACGCCACGACCG
AGAAATTCGGCACCGTGATCGAGAACATGACCTTCGATCCGGAGACTTTTGATCTCAACTTCGATGATGACAGCCTGACG
GCCAACATGCGCTGCGCCTACCCGCTGCATTATATCTCGAATGCATCTGCTTCGGCGCGTGGCGGACATCCCAAGAACAT
CATCATGCTCACCTGTGATGCCTTTGGCGTGCTGCCTCCGATTGCCCGTCTGACGCCGGCCCAGGCGATGTACCACTTCC
TCTCCGGCTTCACTTCCAAGGTCGCCGGGACCGAGCGTGGCGTCACCGAGCCGGAGCCCACGTTCTCCACCTGCTTTGGC
GCGCCCTTCATGCCGCGTCGCCCCGAGGTCTATGGCAACCTCCTGCGCGAAAAGATCGCGGCCCATGGTGCAACCTGCTG
GCTGGTGAACACCGGCTGGACCGGCGGCGCCTATGGCACGGGTTCCCGCATGCCGATCAAAGCGACCCGTGCGCTTTTGA
CTGCTGCGCTTGATGGCTCGCTGGCAAATGCAGAGTTCCGCAAAGATCCGAACTTTGGCTTTGACGTGCCAGTCGCTGCT
CCCGGCGTTGCCGAAGTGCTGCTCGACCCGCGCCGCACATGGGACGACAAAGCCGCCTATGACGCGCAGGCCGAGAAGCT
GGTGCAGATGTTCTCTGACAACTTCGAACAGTACCTGCCCTACATCGATGAGGACGTGAAAGCAGCCGCCATCTGCTGA

Upstream 100 bases:

>100_bases
TTATTGCGCTCCGGCGCCCTATGGCGCAGCCTTTGATCTATGCGGTCCCTGTCACAGCTCCATTGCATCATGCAGGGCCG
ACACCAGGAGAGACAACAAG

Downstream 100 bases:

>100_bases
TCGACGCTTCCCGCAAAAAATACAAAGCCCTCTCCGTTCAATCCGGAGGGGGCTTTTTTGTGTTCCGCGATAAGGGGGCG
GCAGGGCTTAGCCCCGCAAG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTSGRVNPNFRLEDQGIEGLGNVYYNLMEPALVQEALSRGEGNLGKGGAFLVTTGKFTGRSPKDKHVVKTASVADTIWWE
NNAEMSPEGFDALYADMVEHMKGRDYFVQDLVGGADPVHAINVRMVTELAWHNLFIRHLLRRPDREDLDDFIADFTIINC
PSFQADPNKHNCRSETVIALNFDRKMILIGGTEYAGENKKSVFTLLNYLLPEKGIMPMHCSANHAKGNPVDTAVFFGLSG
TGKTTLSADPDRVLIGDDEHGWASNGTFNFEGGCYAKTINLNPEAEPEIYATTEKFGTVIENMTFDPETFDLNFDDDSLT
ANMRCAYPLHYISNASASARGGHPKNIIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFG
APFMPRRPEVYGNLLREKIAAHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLANAEFRKDPNFGFDVPVAA
PGVAEVLLDPRRTWDDKAAYDAQAEKLVQMFSDNFEQYLPYIDEDVKAAAIC

Sequences:

>Translated_532_residues
MTSGRVNPNFRLEDQGIEGLGNVYYNLMEPALVQEALSRGEGNLGKGGAFLVTTGKFTGRSPKDKHVVKTASVADTIWWE
NNAEMSPEGFDALYADMVEHMKGRDYFVQDLVGGADPVHAINVRMVTELAWHNLFIRHLLRRPDREDLDDFIADFTIINC
PSFQADPNKHNCRSETVIALNFDRKMILIGGTEYAGENKKSVFTLLNYLLPEKGIMPMHCSANHAKGNPVDTAVFFGLSG
TGKTTLSADPDRVLIGDDEHGWASNGTFNFEGGCYAKTINLNPEAEPEIYATTEKFGTVIENMTFDPETFDLNFDDDSLT
ANMRCAYPLHYISNASASARGGHPKNIIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFG
APFMPRRPEVYGNLLREKIAAHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLANAEFRKDPNFGFDVPVAA
PGVAEVLLDPRRTWDDKAAYDAQAEKLVQMFSDNFEQYLPYIDEDVKAAAIC
>Mature_531_residues
TSGRVNPNFRLEDQGIEGLGNVYYNLMEPALVQEALSRGEGNLGKGGAFLVTTGKFTGRSPKDKHVVKTASVADTIWWEN
NAEMSPEGFDALYADMVEHMKGRDYFVQDLVGGADPVHAINVRMVTELAWHNLFIRHLLRRPDREDLDDFIADFTIINCP
SFQADPNKHNCRSETVIALNFDRKMILIGGTEYAGENKKSVFTLLNYLLPEKGIMPMHCSANHAKGNPVDTAVFFGLSGT
GKTTLSADPDRVLIGDDEHGWASNGTFNFEGGCYAKTINLNPEAEPEIYATTEKFGTVIENMTFDPETFDLNFDDDSLTA
NMRCAYPLHYISNASASARGGHPKNIIMLTCDAFGVLPPIARLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGA
PFMPRRPEVYGNLLREKIAAHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLANAEFRKDPNFGFDVPVAAP
GVAEVLLDPRRTWDDKAAYDAQAEKLVQMFSDNFEQYLPYIDEDVKAAAIC

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=523, Percent_Identity=47.2275334608031, Blast_Score=457, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322950, Length=535, Percent_Identity=43.7383177570093, Blast_Score=445, Evalue=1e-125,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_SILST (Q1GDU2)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614436.1
- ProteinModelPortal:   Q1GDU2
- SMR:   Q1GDU2
- STRING:   Q1GDU2
- GeneID:   4076924
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2442
- NMPDR:   fig|292414.1.peg.837
- eggNOG:   COG1866
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- PhylomeDB:   Q1GDU2
- ProtClustDB:   PRK09344
- BioCyc:   SSP292414:TM1040_2442-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58436; Mature: 58305

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSGRVNPNFRLEDQGIEGLGNVYYNLMEPALVQEALSRGEGNLGKGGAFLVTTGKFTGR
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SPKDKHVVKTASVADTIWWENNAEMSPEGFDALYADMVEHMKGRDYFVQDLVGGADPVHA
CCCCCCEEEHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEE
INVRMVTELAWHNLFIRHLLRRPDREDLDDFIADFTIINCPSFQADPNKHNCRSETVIAL
EEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEE
NFDRKMILIGGTEYAGENKKSVFTLLNYLLPEKGIMPMHCSANHAKGNPVDTAVFFGLSG
ECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECC
TGKTTLSADPDRVLIGDDEHGWASNGTFNFEGGCYAKTINLNPEAEPEIYATTEKFGTVI
CCCEEECCCCCEEEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCEEEEHHHHHHHH
ENMTFDPETFDLNFDDDSLTANMRCAYPLHYISNASASARGGHPKNIIMLTCDAFGVLPP
HCCCCCCCEEEECCCCCCCEECEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCC
IARLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGAPFMPRRPEVYGNLLREKIA
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
AHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLANAEFRKDPNFGFDVPVAA
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCC
PGVAEVLLDPRRTWDDKAAYDAQAEKLVQMFSDNFEQYLPYIDEDVKAAAIC
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure 
TSGRVNPNFRLEDQGIEGLGNVYYNLMEPALVQEALSRGEGNLGKGGAFLVTTGKFTGR
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
SPKDKHVVKTASVADTIWWENNAEMSPEGFDALYADMVEHMKGRDYFVQDLVGGADPVHA
CCCCCCEEEHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEE
INVRMVTELAWHNLFIRHLLRRPDREDLDDFIADFTIINCPSFQADPNKHNCRSETVIAL
EEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEEE
NFDRKMILIGGTEYAGENKKSVFTLLNYLLPEKGIMPMHCSANHAKGNPVDTAVFFGLSG
ECCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEEEECC
TGKTTLSADPDRVLIGDDEHGWASNGTFNFEGGCYAKTINLNPEAEPEIYATTEKFGTVI
CCCEEECCCCCEEEECCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCEEEEHHHHHHHH
ENMTFDPETFDLNFDDDSLTANMRCAYPLHYISNASASARGGHPKNIIMLTCDAFGVLPP
HCCCCCCCEEEECCCCCCCEECEEEECCHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCC
IARLTPAQAMYHFLSGFTSKVAGTERGVTEPEPTFSTCFGAPFMPRRPEVYGNLLREKIA
HHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHH
AHGATCWLVNTGWTGGAYGTGSRMPIKATRALLTAALDGSLANAEFRKDPNFGFDVPVAA
HCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCC
PGVAEVLLDPRRTWDDKAAYDAQAEKLVQMFSDNFEQYLPYIDEDVKAAAIC
CCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA