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The map label for this gene is nadE [H]

Identifier: 99082118

GI number: 99082118

Start: 2394154

End: 2395815

Strand: Reverse

Name: nadE [H]

Synonym: TM1040_2278

Alternate gene names: 99082118

Gene position: 2395815-2394154 (Counterclockwise)

Preceding gene: 99082121

Following gene: 99082117

Centisome position: 74.85

GC content: 60.65

Gene sequence:

>1662_bases
ATGATGGCCGATAGTTTTCGCATCACCCTTGCGCAATTGAATCCGACGGTTGGGGATTTGGCGGGCAATGCCGCCAAGGC
TCTCTCGGCCTGGCAGGAGGGTCGCGCGGCGGATGCGGATCTCGTGGCGCTGCCGGAGATGTTCATCACCGGCTACAACA
CGCAGGATCTGGTGATGAAACCGGCCTTTCATCAGGCTGCGATCGCTGAGGTCGAGGCGCTTGCGAAGGCCACTGCAGAG
GGTCCTGCCTTGGCCATCGGCAGCCCCTGGGTCGAGGACGGCAAGCTCTACAATGCCTATCTGATCCTCAAGGGCGGCAA
GATCGCCTCCAAATGCCTCAAGCACCATCTTCCCAATGAGACGGTGTTTGACGAGGTTCGGATCTTTGACGCAGGCCCGC
TGGGCGGGCCGTATTCCGTGGGCAACACCCGCATCGGCAGCCCGATCTGCGAGGATGGCTGGCACGAGGATGTCGCCGAG
ACGCTGGAAGAAACCGGCGCTGAGTTCCTGCTCATTCCCAATGGCTCGCCCTATTACCGCGGCAAGATGGAAACCCGCAT
CAACCACATGGTTGCTCGGGCGGTCGAAACGGGCCTGCCGGTGATCTACCTCAACATGGTGGGCGGTCAGGACGATCAGG
TCTTTGATGGGGGCAGCTTTGCGCTCAACCCGCATGGGGCTTTGGCGGTGCAGATGCCGGTCTTTGACGAATGCATCGCA
CAGCTTGATCTCGAGCGCACCGCCGATGGCTGGCGCATCAAAGAGGGCGAAAAGGCGCATCTACCCGATGCGTGGGAGCA
GGACTATCGCACCATGGTGCTGTCCTTGCGCGATTACATGGGCAAGACCGGCTTCAAAAAAGCGCTGCTGGGCCTCTCGG
GGGGGGTGGATTCCGCCATCGTTGCGGCCATCGCCGTCGATGCCTTGGGCGCGGAAAACGTGCGCTGCGTCATGCTGCCG
TCGGAATATACCTCCAAGGAATCACTCGAGGATGCAGAGGCCGTCGCCAAGGCGCTGGGCGTCCACTACGACTATGTGCC
GATCTCTGAGGGCCGCGAAGCAATCACCAACACGCTTGCGCCGCTCTTTGCGGGACGGGACGCGGATCTCACCGAGGAAA
ACATCCAGTCCCGCCTGCGCGGGCTTCTGCTAATGGCCATGTCGAACAAATTTGGCGAGATGCTCCTGACCACCGGCAAT
AAATCCGAGGTCGCGGTGGGCTATGCCACCATCTACGGCGATATGAACGGCGGTTATAACCCGATCAAGGATCTCTACAA
GACGCGTGTGTTTGAAACCTGCCGCTGGCGCAATGCCAATCACCGCGACTGGATGATGGGGCCAGAGGGCGAGGTGATCC
GCCCCAATGTGATCGACAAGCCCCCCTCAGCCGAGCTGCGCGAGGACCAGAAGGACAGCGATTCCCTGCCGGACTATCCC
GAGCTCGATGCGATTCTCGACATTCTCGTGGATCAGGAAGGATCAATCGCCGATTGCGTCGCCGCAGGCTTTGACCGCGA
CGTGGCAAAACGGGTCGAACACCTGCTTTATATCAGCGAATACAAGCGCTTTCAGTCCGCTCCCGGCGCGCGGCTGACCA
AGCGGGCCTTCTGGCTTGATCGGCGTTATCCGATTGTCAATCGCTGGCGTGATCCCAGCTGA

Upstream 100 bases:

>100_bases
GTCAGTGCGGGGCCTGTCGCAATGGTTTTGCCCCCCGGAGGCTTTCCCTTCGGCGGGGATCTGATACATGGCAGGGTAAG
AGATGTTTTCGAGGACCCCG

Downstream 100 bases:

>100_bases
AAATCCCTGCCACCGTCTCGTAAAACACCTGTGTTCTGGCCGTGATTGAGGCCCCGAGAACACAGGCTGCGGGAACCGGT
CAAATGAGCTTCATGCGCGG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MMADSFRITLAQLNPTVGDLAGNAAKALSAWQEGRAADADLVALPEMFITGYNTQDLVMKPAFHQAAIAEVEALAKATAE
GPALAIGSPWVEDGKLYNAYLILKGGKIASKCLKHHLPNETVFDEVRIFDAGPLGGPYSVGNTRIGSPICEDGWHEDVAE
TLEETGAEFLLIPNGSPYYRGKMETRINHMVARAVETGLPVIYLNMVGGQDDQVFDGGSFALNPHGALAVQMPVFDECIA
QLDLERTADGWRIKEGEKAHLPDAWEQDYRTMVLSLRDYMGKTGFKKALLGLSGGVDSAIVAAIAVDALGAENVRCVMLP
SEYTSKESLEDAEAVAKALGVHYDYVPISEGREAITNTLAPLFAGRDADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGN
KSEVAVGYATIYGDMNGGYNPIKDLYKTRVFETCRWRNANHRDWMMGPEGEVIRPNVIDKPPSAELREDQKDSDSLPDYP
ELDAILDILVDQEGSIADCVAAGFDRDVAKRVEHLLYISEYKRFQSAPGARLTKRAFWLDRRYPIVNRWRDPS

Sequences:

>Translated_553_residues
MMADSFRITLAQLNPTVGDLAGNAAKALSAWQEGRAADADLVALPEMFITGYNTQDLVMKPAFHQAAIAEVEALAKATAE
GPALAIGSPWVEDGKLYNAYLILKGGKIASKCLKHHLPNETVFDEVRIFDAGPLGGPYSVGNTRIGSPICEDGWHEDVAE
TLEETGAEFLLIPNGSPYYRGKMETRINHMVARAVETGLPVIYLNMVGGQDDQVFDGGSFALNPHGALAVQMPVFDECIA
QLDLERTADGWRIKEGEKAHLPDAWEQDYRTMVLSLRDYMGKTGFKKALLGLSGGVDSAIVAAIAVDALGAENVRCVMLP
SEYTSKESLEDAEAVAKALGVHYDYVPISEGREAITNTLAPLFAGRDADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGN
KSEVAVGYATIYGDMNGGYNPIKDLYKTRVFETCRWRNANHRDWMMGPEGEVIRPNVIDKPPSAELREDQKDSDSLPDYP
ELDAILDILVDQEGSIADCVAAGFDRDVAKRVEHLLYISEYKRFQSAPGARLTKRAFWLDRRYPIVNRWRDPS
>Mature_553_residues
MMADSFRITLAQLNPTVGDLAGNAAKALSAWQEGRAADADLVALPEMFITGYNTQDLVMKPAFHQAAIAEVEALAKATAE
GPALAIGSPWVEDGKLYNAYLILKGGKIASKCLKHHLPNETVFDEVRIFDAGPLGGPYSVGNTRIGSPICEDGWHEDVAE
TLEETGAEFLLIPNGSPYYRGKMETRINHMVARAVETGLPVIYLNMVGGQDDQVFDGGSFALNPHGALAVQMPVFDECIA
QLDLERTADGWRIKEGEKAHLPDAWEQDYRTMVLSLRDYMGKTGFKKALLGLSGGVDSAIVAAIAVDALGAENVRCVMLP
SEYTSKESLEDAEAVAKALGVHYDYVPISEGREAITNTLAPLFAGRDADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGN
KSEVAVGYATIYGDMNGGYNPIKDLYKTRVFETCRWRNANHRDWMMGPEGEVIRPNVIDKPPSAELREDQKDSDSLPDYP
ELDAILDILVDQEGSIADCVAAGFDRDVAKRVEHLLYISEYKRFQSAPGARLTKRAFWLDRRYPIVNRWRDPS

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=582, Percent_Identity=23.0240549828179, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 60772; Mature: 60772

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADSFRITLAQLNPTVGDLAGNAAKALSAWQEGRAADADLVALPEMFITGYNTQDLVMK
CCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCHHHEEC
PAFHQAAIAEVEALAKATAEGPALAIGSPWVEDGKLYNAYLILKGGKIASKCLKHHLPNE
CCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCCC
TVFDEVRIFDAGPLGGPYSVGNTRIGSPICEDGWHEDVAETLEETGAEFLLIPNGSPYYR
CHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
GKMETRINHMVARAVETGLPVIYLNMVGGQDDQVFDGGSFALNPHGALAVQMPVFDECIA
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCEEECCCCCEEEECCHHHHHHH
QLDLERTADGWRIKEGEKAHLPDAWEQDYRTMVLSLRDYMGKTGFKKALLGLSGGVDSAI
HHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHH
VAAIAVDALGAENVRCVMLPSEYTSKESLEDAEAVAKALGVHYDYVPISEGREAITNTLA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
PLFAGRDADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNKSEVAVGYATIYGDMNGGYN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCC
PIKDLYKTRVFETCRWRNANHRDWMMGPEGEVIRPNVIDKPPSAELREDQKDSDSLPDYP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCC
ELDAILDILVDQEGSIADCVAAGFDRDVAKRVEHLLYISEYKRFQSAPGARLTKRAFWLD
CHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
RRYPIVNRWRDPS
CCCCHHCCCCCCC
>Mature Secondary Structure
MMADSFRITLAQLNPTVGDLAGNAAKALSAWQEGRAADADLVALPEMFITGYNTQDLVMK
CCCCCEEEEEEECCCCHHHHCCHHHHHHHHHHCCCCCCCCHHCCCHHHHCCCCCHHHEEC
PAFHQAAIAEVEALAKATAEGPALAIGSPWVEDGKLYNAYLILKGGKIASKCLKHHLPNE
CCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCCC
TVFDEVRIFDAGPLGGPYSVGNTRIGSPICEDGWHEDVAETLEETGAEFLLIPNGSPYYR
CHHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCC
GKMETRINHMVARAVETGLPVIYLNMVGGQDDQVFDGGSFALNPHGALAVQMPVFDECIA
CHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCCEEECCCCCEEEECCHHHHHHH
QLDLERTADGWRIKEGEKAHLPDAWEQDYRTMVLSLRDYMGKTGFKKALLGLSGGVDSAI
HHHHHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHH
VAAIAVDALGAENVRCVMLPSEYTSKESLEDAEAVAKALGVHYDYVPISEGREAITNTLA
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
PLFAGRDADLTEENIQSRLRGLLLMAMSNKFGEMLLTTGNKSEVAVGYATIYGDMNGGYN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEEEEECCCCCCC
PIKDLYKTRVFETCRWRNANHRDWMMGPEGEVIRPNVIDKPPSAELREDQKDSDSLPDYP
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCCCCCCCCCCCC
ELDAILDILVDQEGSIADCVAAGFDRDVAKRVEHLLYISEYKRFQSAPGARLTKRAFWLD
CHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHC
RRYPIVNRWRDPS
CCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]