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The map label for this gene is nadB [H]

Identifier: 99082089

GI number: 99082089

Start: 2362822

End: 2364417

Strand: Reverse

Name: nadB [H]

Synonym: TM1040_2249

Alternate gene names: 99082089

Gene position: 2364417-2362822 (Counterclockwise)

Preceding gene: 99082090

Following gene: 99082088

Centisome position: 73.87

GC content: 66.79

Gene sequence:

>1596_bases
ATGGAAAGCTCTCAGGACATCCAAAGCAACCGCATCGTCATCATCGGTGCGGGGCTGGGGGCGCTTTATGCGGCGTTGAC
GCTCGCGCCGCGCCCGGTGGTGATGATTTCCCCGGATCCCTTGGGGCAGGGGGCCAGTTCGGCCTGGGCGCAGGGTGGTG
TTGCGGCAGCAATGGACGTGAGCGACAGCCCTGCGGCGCATGCGCGCGACACCCAACGCGCTGGGGCGGGCACAGTGGAC
GCGCAAGTGGCGGATCTGGTCACACGCGAGGCGCGCGAGCATATCCTTGATCTCACCACATTGGGCGCGCCTTTTGATCG
TACTGCCGACGGCAGCTATGTGATGTCGCGCGAAGCGGCGCACAGCTATGCCCGCGTGGTGCGCGTGAAGGGCGATCAGG
CCGGCGCGGAGATCATGCGCGCCCTCATCGAACGGCTCCGGGAAACGCCCTCGATCCAGGTGATCGAAGGGGCGATGGCC
GAAGGGCTGGAACTTTCGGATGGCGCGGTGACGGGGGTGCGACTGGCCTCCTCCGCACCCTCTGGATCGGCACCGCTGGT
CTTGCGCGGTCAGGCCGTTTTGATGGCCGGGGGCGGCAGCGCCGGGCTTTATGCCATTACCACCAATCCGCCCCGTATCC
GGGGGCAGGCGCTGGGCATGGCGGCGCGCGCGGGCGCCGAGATCGCCGATGCGGAGTTCATGCAGTTCCACCCCACGGCG
ATGGATGTCGGCGAAGACCCGGCACCGCTCGCAACAGAGGCCCTGCGCGGTGAGGGCGCAATCCTGGTGAACGCAGATGG
TGCGCGCTTCATGCTGGACGAACATCCCGACGCAGAACTGGCCCCACGCGATGTGGTGGCGCGGGCGATCTTTGCGCAGT
CCATCGCAGGAAAACGCCCGCTTCTGGACACGCGGGCCGCTTTGGGTGAGGCCATCACCACCGTGTTTCCAGCCGTCAGC
GCGGCCTGCGCGCGCAATGGTATCGACCCCACCCAAGACCCAATCCCGGTTGCGGCGGCCGCGCATTACCACATGGGCGG
CATTGCCACCGATCTCGACGGAAAGAGCTCGCTTCCGGGGCTCTGGGCCTGCGGTGAATGTGCCTCCACCGGGCTGCATG
GCGCCAATCGTCTTGCCTCCAACGGGCTTCTGGAGGCGCTGGTTTACGCACGCCGCGCCGCCCAGGACATCGCACGCACG
CTGGGTCCCCTCAAAGAGGCCACGTCTGTGCGCCTTGAATTTGCGCCGGGTGGTGCAACAACTGATCCAGCCCATGTCGC
GGCCCTGCGCCGTGCAATGACACAGAATGTGGGCGTGGTGCGCGATGCAGCCGGGCTGCGCGCGGCCCTCAGCGAGATTG
CGGCCCTCGCAGCAACGCAGCCCGACTGCGAAAGCTTTCAAAACATCTGTACCGCCGCTACCATGATCGCCGCCGCCGCC
TTGTTGCGCGAAGAAAGCCGTGGCGCGCATGAGCGGTCCGATTTTCCCGAAACCCTGCCTGCGCTTGCGCATCGCTCCCG
CCTCACGTGGGAGGCAGCCAAAGCGCTCCGTGACAGCCTGTGTGATGCCAGTGCATCCGACCCGGAGACCCCATGA

Upstream 100 bases:

>100_bases
GAGGGTCAGGTCGAGGTCGAGCCCGAAATGGCCGCCAAGGCGCGACACGCGGTGCAGGCGATGATCGACCTGTCGCGCAA
GCTGGGCATCTAAGCGCAGC

Downstream 100 bases:

>100_bases
GCTTTGCCACTTTGCCCGACCTGATCCTTGAGCCATTGGTCAAATCCGCCCTGATGGAAGACCTCGGCTCTTATGGCGAT
GTGACCACCCGCGCGGTGAT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MESSQDIQSNRIVIIGAGLGALYAALTLAPRPVVMISPDPLGQGASSAWAQGGVAAAMDVSDSPAAHARDTQRAGAGTVD
AQVADLVTREAREHILDLTTLGAPFDRTADGSYVMSREAAHSYARVVRVKGDQAGAEIMRALIERLRETPSIQVIEGAMA
EGLELSDGAVTGVRLASSAPSGSAPLVLRGQAVLMAGGGSAGLYAITTNPPRIRGQALGMAARAGAEIADAEFMQFHPTA
MDVGEDPAPLATEALRGEGAILVNADGARFMLDEHPDAELAPRDVVARAIFAQSIAGKRPLLDTRAALGEAITTVFPAVS
AACARNGIDPTQDPIPVAAAAHYHMGGIATDLDGKSSLPGLWACGECASTGLHGANRLASNGLLEALVYARRAAQDIART
LGPLKEATSVRLEFAPGGATTDPAHVAALRRAMTQNVGVVRDAAGLRAALSEIAALAATQPDCESFQNICTAATMIAAAA
LLREESRGAHERSDFPETLPALAHRSRLTWEAAKALRDSLCDASASDPETP

Sequences:

>Translated_531_residues
MESSQDIQSNRIVIIGAGLGALYAALTLAPRPVVMISPDPLGQGASSAWAQGGVAAAMDVSDSPAAHARDTQRAGAGTVD
AQVADLVTREAREHILDLTTLGAPFDRTADGSYVMSREAAHSYARVVRVKGDQAGAEIMRALIERLRETPSIQVIEGAMA
EGLELSDGAVTGVRLASSAPSGSAPLVLRGQAVLMAGGGSAGLYAITTNPPRIRGQALGMAARAGAEIADAEFMQFHPTA
MDVGEDPAPLATEALRGEGAILVNADGARFMLDEHPDAELAPRDVVARAIFAQSIAGKRPLLDTRAALGEAITTVFPAVS
AACARNGIDPTQDPIPVAAAAHYHMGGIATDLDGKSSLPGLWACGECASTGLHGANRLASNGLLEALVYARRAAQDIART
LGPLKEATSVRLEFAPGGATTDPAHVAALRRAMTQNVGVVRDAAGLRAALSEIAALAATQPDCESFQNICTAATMIAAAA
LLREESRGAHERSDFPETLPALAHRSRLTWEAAKALRDSLCDASASDPETP
>Mature_531_residues
MESSQDIQSNRIVIIGAGLGALYAALTLAPRPVVMISPDPLGQGASSAWAQGGVAAAMDVSDSPAAHARDTQRAGAGTVD
AQVADLVTREAREHILDLTTLGAPFDRTADGSYVMSREAAHSYARVVRVKGDQAGAEIMRALIERLRETPSIQVIEGAMA
EGLELSDGAVTGVRLASSAPSGSAPLVLRGQAVLMAGGGSAGLYAITTNPPRIRGQALGMAARAGAEIADAEFMQFHPTA
MDVGEDPAPLATEALRGEGAILVNADGARFMLDEHPDAELAPRDVVARAIFAQSIAGKRPLLDTRAALGEAITTVFPAVS
AACARNGIDPTQDPIPVAAAAHYHMGGIATDLDGKSSLPGLWACGECASTGLHGANRLASNGLLEALVYARRAAQDIART
LGPLKEATSVRLEFAPGGATTDPAHVAALRRAMTQNVGVVRDAAGLRAALSEIAALAATQPDCESFQNICTAATMIAAAA
LLREESRGAHERSDFPETLPALAHRSRLTWEAAKALRDSLCDASASDPETP

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=502, Percent_Identity=29.2828685258964, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI1788928, Length=523, Percent_Identity=37.093690248566, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1790597, Length=495, Percent_Identity=30.7070707070707, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1786942, Length=508, Percent_Identity=29.1338582677165, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17550100, Length=526, Percent_Identity=28.8973384030418, Blast_Score=183, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17505833, Length=525, Percent_Identity=28.5714285714286, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=459, Percent_Identity=27.0152505446623, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=523, Percent_Identity=30.7839388145315, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6322416, Length=521, Percent_Identity=30.9021113243762, Blast_Score=187, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6322511, Length=330, Percent_Identity=27.8787878787879, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320788, Length=337, Percent_Identity=27.893175074184, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137288, Length=504, Percent_Identity=30.5555555555556, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655642, Length=504, Percent_Identity=30.5555555555556, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24655647, Length=504, Percent_Identity=30.5555555555556, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24663005, Length=529, Percent_Identity=29.6786389413989, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 54722; Mature: 54722

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSQDIQSNRIVIIGAGLGALYAALTLAPRPVVMISPDPLGQGASSAWAQGGVAAAMDV
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCEEEEECC
SDSPAAHARDTQRAGAGTVDAQVADLVTREAREHILDLTTLGAPFDRTADGSYVMSREAA
CCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHH
HSYARVVRVKGDQAGAEIMRALIERLRETPSIQVIEGAMAEGLELSDGAVTGVRLASSAP
HHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCCEEEEEEECCCC
SGSAPLVLRGQAVLMAGGGSAGLYAITTNPPRIRGQALGMAARAGAEIADAEFMQFHPTA
CCCCCEEEECCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHCCHHHHCCCCH
MDVGEDPAPLATEALRGEGAILVNADGARFMLDEHPDAELAPRDVVARAIFAQSIAGKRP
HCCCCCCCCHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
LLDTRAALGEAITTVFPAVSAACARNGIDPTQDPIPVAAAAHYHMGGIATDLDGKSSLPG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCC
LWACGECASTGLHGANRLASNGLLEALVYARRAAQDIARTLGPLKEATSVRLEFAPGGAT
CEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
TDPAHVAALRRAMTQNVGVVRDAAGLRAALSEIAALAATQPDCESFQNICTAATMIAAAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LLREESRGAHERSDFPETLPALAHRSRLTWEAAKALRDSLCDASASDPETP
HHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MESSQDIQSNRIVIIGAGLGALYAALTLAPRPVVMISPDPLGQGASSAWAQGGVAAAMDV
CCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHCCCEEEEECC
SDSPAAHARDTQRAGAGTVDAQVADLVTREAREHILDLTTLGAPFDRTADGSYVMSREAA
CCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHH
HSYARVVRVKGDQAGAEIMRALIERLRETPSIQVIEGAMAEGLELSDGAVTGVRLASSAP
HHHHHEEEECCCHHHHHHHHHHHHHHHCCCCEEEECHHHHCCCCCCCCCEEEEEEECCCC
SGSAPLVLRGQAVLMAGGGSAGLYAITTNPPRIRGQALGMAARAGAEIADAEFMQFHPTA
CCCCCEEEECCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHCCHHHHCCCCH
MDVGEDPAPLATEALRGEGAILVNADGARFMLDEHPDAELAPRDVVARAIFAQSIAGKRP
HCCCCCCCCHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCC
LLDTRAALGEAITTVFPAVSAACARNGIDPTQDPIPVAAAAHYHMGGIATDLDGKSSLPG
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCC
LWACGECASTGLHGANRLASNGLLEALVYARRAAQDIARTLGPLKEATSVRLEFAPGGAT
CEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCC
TDPAHVAALRRAMTQNVGVVRDAAGLRAALSEIAALAATQPDCESFQNICTAATMIAAAA
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LLREESRGAHERSDFPETLPALAHRSRLTWEAAKALRDSLCDASASDPETP
HHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]