| Definition | Ruegeria sp. TM1040, complete genome. |
|---|---|
| Accession | NC_008044 |
| Length | 3,200,938 |
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The map label for this gene is cheR [C]
Identifier: 99082067
GI number: 99082067
Start: 2338410
End: 2340884
Strand: Direct
Name: cheR [C]
Synonym: TM1040_2227
Alternate gene names: 99082067
Gene position: 2338410-2340884 (Clockwise)
Preceding gene: 99082064
Following gene: 99082068
Centisome position: 73.05
GC content: 58.22
Gene sequence:
>2475_bases ATGGCCAAGGCAGAGCTGCCAGTGATCATTGGAATTGCGGCATCGGCCGGCGGGTTGGAGGCAACCACGTCGCTGGTCAA GAATCTGCCGTCGCAGACCAATGCCAGCTATGTCGTCGCTCAGCACATGTCCCCGACTCACAAAAGCCTGTTGTGGTCCC TGATCGGGCGAGAAACCGAACTGCCGGTGATCGAACTCACGGACGAAGACCTGGTCCCGGAGCCCGACACGATCTATATC GCGCCGCCCAATTACGATGTCATCGTGGACAACGGCAAACTGGCCCTGCGTGATCCCAGCGGCCATCCCGGCACGCCGAA ACCCTTGGCGGACCGGCTATTCAAAAGCCTTGCCGACGAATGTGGCGAACGCTGCGTCGGGATCGTTCTGTCCGGTACCG GCAGCGATGGAAGCTACGGTATCCGCGCCATTCGCGAAGCCGGTGGGGTCACCATCGCGCAGGAAATCGGCACCGCCAAA TATGAGGGTATGCCCTCCTCGGCCGTGCATACGGGGTGTATCGATCTGACCATGTCGCCGGATATGATCGGCCAGCAACT CGACAGCATCCTCGCGAACCCTCGGGATTTTTCCGAACTCAGAGGATTTACCGGCAACCCCAGCGGGTTGCTCGATCTCT ACCATATCCTACTTGCGCGCACCGGCGTGGATTTCCGCGAGTACAAGGAAACCACCATCAACCGCCGCATCGCGCGCAGA ATGGTTGCGCAGGGCATCGACGTCTATGAGGACTATGTCGAATTCTGTCGTGAGAACGCAACCGAGGTGGATGCGCTCTA CAAGGACATGATGATCTCAGTGACGCGCTTTTTCCGCGATCACGCCCAGTTCAACCAGCTCACCAAGGTCATTCAGGGTT TGGTGGATGACAACAGAGATCGCCAGATCCGTGTCTGGGTTGCAGGCAGCGCCACCGGCGAAGAAGCCTATTCTCTGGCC ATCCTCTTTGCAGAGGCGATGGGGGGACTAAAGGCAATCAGCAAAGAGCGCATTCAGATCTTTGCCACCGACATCGACAC CGAGGCGCTGGAGGTCGCGCGACGCGGGATCTATCCGCTCTCGGCCGCCAACGACGTGCCGCGCCAATATGTTGAGACCT ACATGAACCTCGAAGGCGACGCGCTGGAGGTCAACAAGCATCTGCGGTCGGTGGTGCTGTTTTCACGGCACAACCTTATT CAGGACCCGCCCTTCATCAACATGGACATGATCAGTCTGCGCAATCTGTTGATCTATTTTGGCGCGTCCCTACAGGAAAA AGTCCTGTCACGCATGGCCTATGCCCTTGTTCCCAATGGGAAACTCTTCCTGGGGTCGTCCGAAACCGTCGGCTCGATGC ATGGGCATTTCGAAACCTATTCCCTGCCCGACAAGATCTATTCCAAACGCGGCCTGCGGCAGAGCGTGCAAGCCACGGCA AATTTCCTGCCCCTTACGGCCCTGCATCGCGGCCCGCTTGCACAGAGCCGTGCATCAACGCCGCCGCAGACCTTCGACAT GTTCGACGCGCTCATCCGGACTTTGGCACCAAATGGGTTTATTGCCACGCAGGACCACGTTATCATCCGCATAATCGGTG ATATTTCCAGCGCCACTGAACTCAACGAGAGCAGCGCGCTGCCGCGCATGTCGACCAAAATCCTGAAATCCGAGCTGCGC GCCGAAGCGGCGAGCCTCATCACTCTTTGCCTGCGCAGCAAATCGACTCGATCAGGGCGCTGGCACGATGTTGAAGGGTC GGACCTGATCCAATTGCGTATGACCTGCTTCCCGCTCTTGATGGATGGCGAGGGTGAAGACCATGTGCTGTTTTCGATCG AAGAGCGTGAATCCCAGCCTCGGACCGTCGCAACCGAGGACATGAATATCGACGACCGAATGGTCTATGTGCGCCAGATC GAGGACGAAATCGCCTCCACCCGCGAGGCGCTGCAGCAGACCATCGAGGAGCTGCAAACTTCGAACGAGGAACTACAGTC CGTCAACGAAGAGATGCAGGCGACCAACGAAGAACTGCAAGCCACCAATGAAGAGCTCGAAACCTCGAATGAGGAGCTTC AGGCCACCAACGAAGAGCTGATCACCGTCAACGAGGAAATGCAGATCAACTCCACCGAGCTGCAACAACTCTCGATTGAA TTGGCCGCTGTTCTCGAGGCGACCCCCTACCCGACGCTGCTGGTCGACCCGGCCCTGCAGATCAGGCATGCCTCGGCAGC AGCGATCGATCATTTTGGCCTCTCCGAGCTGCCCCCAAACGGCACTCATATCGGCGCCTGCACCGATGGTGCAGGGCTGG CGCATCTTGTTTCCAAGGTGGCCGAGTGTTTCCGCACCCGCGAGAGCAGCACGCTCGAGGTCAATGACGAGACCTGGTCA AAGACGCTGACTTTCACACCGATTCAAAAGGGCGTCACCCAGGAAACCATCGGTGTCACTGTCACCGTTCTGTAG
Upstream 100 bases:
>100_bases CTCAGAGAAGATCCCTTAGCGGATAGCAATTCCCCTGACAGCATCTTACTGTCAAATCGTGGTATCGTATTTTGCAATAC CCAATTCAGGAGACAGGCTC
Downstream 100 bases:
>100_bases AACGAATCGCATAATCTTAGCCTGGGAATCGCGCATCACCTTCGAGGGTTCTTGCGCGGCCAAACCCGCAGAGCTGCGAC TCGGGGTGCGGCGTAGAAAA
Product: MCP methyltransferase, CheR-type
Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]
Alternate protein names: ORF19 [H]
Number of amino acids: Translated: 824; Mature: 823
Protein sequence:
>824_residues MAKAELPVIIGIAASAGGLEATTSLVKNLPSQTNASYVVAQHMSPTHKSLLWSLIGRETELPVIELTDEDLVPEPDTIYI APPNYDVIVDNGKLALRDPSGHPGTPKPLADRLFKSLADECGERCVGIVLSGTGSDGSYGIRAIREAGGVTIAQEIGTAK YEGMPSSAVHTGCIDLTMSPDMIGQQLDSILANPRDFSELRGFTGNPSGLLDLYHILLARTGVDFREYKETTINRRIARR MVAQGIDVYEDYVEFCRENATEVDALYKDMMISVTRFFRDHAQFNQLTKVIQGLVDDNRDRQIRVWVAGSATGEEAYSLA ILFAEAMGGLKAISKERIQIFATDIDTEALEVARRGIYPLSAANDVPRQYVETYMNLEGDALEVNKHLRSVVLFSRHNLI QDPPFINMDMISLRNLLIYFGASLQEKVLSRMAYALVPNGKLFLGSSETVGSMHGHFETYSLPDKIYSKRGLRQSVQATA NFLPLTALHRGPLAQSRASTPPQTFDMFDALIRTLAPNGFIATQDHVIIRIIGDISSATELNESSALPRMSTKILKSELR AEAASLITLCLRSKSTRSGRWHDVEGSDLIQLRMTCFPLLMDGEGEDHVLFSIEERESQPRTVATEDMNIDDRMVYVRQI EDEIASTREALQQTIEELQTSNEELQSVNEEMQATNEELQATNEELETSNEELQATNEELITVNEEMQINSTELQQLSIE LAAVLEATPYPTLLVDPALQIRHASAAAIDHFGLSELPPNGTHIGACTDGAGLAHLVSKVAECFRTRESSTLEVNDETWS KTLTFTPIQKGVTQETIGVTVTVL
Sequences:
>Translated_824_residues MAKAELPVIIGIAASAGGLEATTSLVKNLPSQTNASYVVAQHMSPTHKSLLWSLIGRETELPVIELTDEDLVPEPDTIYI APPNYDVIVDNGKLALRDPSGHPGTPKPLADRLFKSLADECGERCVGIVLSGTGSDGSYGIRAIREAGGVTIAQEIGTAK YEGMPSSAVHTGCIDLTMSPDMIGQQLDSILANPRDFSELRGFTGNPSGLLDLYHILLARTGVDFREYKETTINRRIARR MVAQGIDVYEDYVEFCRENATEVDALYKDMMISVTRFFRDHAQFNQLTKVIQGLVDDNRDRQIRVWVAGSATGEEAYSLA ILFAEAMGGLKAISKERIQIFATDIDTEALEVARRGIYPLSAANDVPRQYVETYMNLEGDALEVNKHLRSVVLFSRHNLI QDPPFINMDMISLRNLLIYFGASLQEKVLSRMAYALVPNGKLFLGSSETVGSMHGHFETYSLPDKIYSKRGLRQSVQATA NFLPLTALHRGPLAQSRASTPPQTFDMFDALIRTLAPNGFIATQDHVIIRIIGDISSATELNESSALPRMSTKILKSELR AEAASLITLCLRSKSTRSGRWHDVEGSDLIQLRMTCFPLLMDGEGEDHVLFSIEERESQPRTVATEDMNIDDRMVYVRQI EDEIASTREALQQTIEELQTSNEELQSVNEEMQATNEELQATNEELETSNEELQATNEELITVNEEMQINSTELQQLSIE LAAVLEATPYPTLLVDPALQIRHASAAAIDHFGLSELPPNGTHIGACTDGAGLAHLVSKVAECFRTRESSTLEVNDETWS KTLTFTPIQKGVTQETIGVTVTVL >Mature_823_residues AKAELPVIIGIAASAGGLEATTSLVKNLPSQTNASYVVAQHMSPTHKSLLWSLIGRETELPVIELTDEDLVPEPDTIYIA PPNYDVIVDNGKLALRDPSGHPGTPKPLADRLFKSLADECGERCVGIVLSGTGSDGSYGIRAIREAGGVTIAQEIGTAKY EGMPSSAVHTGCIDLTMSPDMIGQQLDSILANPRDFSELRGFTGNPSGLLDLYHILLARTGVDFREYKETTINRRIARRM VAQGIDVYEDYVEFCRENATEVDALYKDMMISVTRFFRDHAQFNQLTKVIQGLVDDNRDRQIRVWVAGSATGEEAYSLAI LFAEAMGGLKAISKERIQIFATDIDTEALEVARRGIYPLSAANDVPRQYVETYMNLEGDALEVNKHLRSVVLFSRHNLIQ DPPFINMDMISLRNLLIYFGASLQEKVLSRMAYALVPNGKLFLGSSETVGSMHGHFETYSLPDKIYSKRGLRQSVQATAN FLPLTALHRGPLAQSRASTPPQTFDMFDALIRTLAPNGFIATQDHVIIRIIGDISSATELNESSALPRMSTKILKSELRA EAASLITLCLRSKSTRSGRWHDVEGSDLIQLRMTCFPLLMDGEGEDHVLFSIEERESQPRTVATEDMNIDDRMVYVRQIE DEIASTREALQQTIEELQTSNEELQSVNEEMQATNEELQATNEELETSNEELQATNEELITVNEEMQINSTELQQLSIEL AAVLEATPYPTLLVDPALQIRHASAAAIDHFGLSELPPNGTHIGACTDGAGLAHLVSKVAECFRTRESSTLEVNDETWSK TLTFTPIQKGVTQETIGVTVTVL
Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]
COG id: COG1352
COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cheR-type methyltransferase domain [H]
Homologues:
Organism=Escherichia coli, GI1788193, Length=255, Percent_Identity=31.3725490196078, Blast_Score=109, Evalue=7e-25, Organism=Escherichia coli, GI1788192, Length=190, Percent_Identity=30, Blast_Score=83, Evalue=6e-17,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022642 - InterPro: IPR000780 - InterPro: IPR022641 - InterPro: IPR000014 - InterPro: IPR013656 - InterPro: IPR000673 [H]
Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]
EC number: 2.1.1.80 [C]
Molecular weight: Translated: 90853; Mature: 90722
Theoretical pI: Translated: 4.53; Mature: 4.53
Prosite motif: PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.6 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAKAELPVIIGIAASAGGLEATTSLVKNLPSQTNASYVVAQHMSPTHKSLLWSLIGRETE CCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCC LPVIELTDEDLVPEPDTIYIAPPNYDVIVDNGKLALRDPSGHPGTPKPLADRLFKSLADE CCEEEECCCCCCCCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHH CGERCVGIVLSGTGSDGSYGIRAIREAGGVTIAQEIGTAKYEGMPSSAVHTGCIDLTMSP HCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHCCHHCCCCCCCCCCCCEEEEEECH DMIGQQLDSILANPRDFSELRGFTGNPSGLLDLYHILLARTGVDFREYKETTINRRIARR HHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH MVAQGIDVYEDYVEFCRENATEVDALYKDMMISVTRFFRDHAQFNQLTKVIQGLVDDNRD HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC RQIRVWVAGSATGEEAYSLAILFAEAMGGLKAISKERIQIFATDIDTEALEVARRGIYPL CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCC SAANDVPRQYVETYMNLEGDALEVNKHLRSVVLFSRHNLIQDPPFINMDMISLRNLLIYF CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH GASLQEKVLSRMAYALVPNGKLFLGSSETVGSMHGHFETYSLPDKIYSKRGLRQSVQATA CCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH NFLPLTALHRGPLAQSRASTPPQTFDMFDALIRTLAPNGFIATQDHVIIRIIGDISSATE HHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHH LNESSALPRMSTKILKSELRAEAASLITLCLRSKSTRSGRWHDVEGSDLIQLRMTCFPLL CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHEEEE MDGEGEDHVLFSIEERESQPRTVATEDMNIDDRMVYVRQIEDEIASTREALQQTIEELQT ECCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC SNEELQSVNEEMQATNEELQATNEELETSNEELQATNEELITVNEEMQINSTELQQLSIE CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH LAAVLEATPYPTLLVDPALQIRHASAAAIDHFGLSELPPNGTHIGACTDGAGLAHLVSKV HHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHH AECFRTRESSTLEVNDETWSKTLTFTPIQKGVTQETIGVTVTVL HHHHHCCCCCEEEECCCHHCCEEEECHHHHCCCCHHCCEEEEEC >Mature Secondary Structure AKAELPVIIGIAASAGGLEATTSLVKNLPSQTNASYVVAQHMSPTHKSLLWSLIGRETE CCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCC LPVIELTDEDLVPEPDTIYIAPPNYDVIVDNGKLALRDPSGHPGTPKPLADRLFKSLADE CCEEEECCCCCCCCCCEEEEECCCCEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHH CGERCVGIVLSGTGSDGSYGIRAIREAGGVTIAQEIGTAKYEGMPSSAVHTGCIDLTMSP HCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHCCHHCCCCCCCCCCCCEEEEEECH DMIGQQLDSILANPRDFSELRGFTGNPSGLLDLYHILLARTGVDFREYKETTINRRIARR HHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH MVAQGIDVYEDYVEFCRENATEVDALYKDMMISVTRFFRDHAQFNQLTKVIQGLVDDNRD HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC RQIRVWVAGSATGEEAYSLAILFAEAMGGLKAISKERIQIFATDIDTEALEVARRGIYPL CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCC SAANDVPRQYVETYMNLEGDALEVNKHLRSVVLFSRHNLIQDPPFINMDMISLRNLLIYF CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH GASLQEKVLSRMAYALVPNGKLFLGSSETVGSMHGHFETYSLPDKIYSKRGLRQSVQATA CCCHHHHHHHHHHHHCCCCCEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH NFLPLTALHRGPLAQSRASTPPQTFDMFDALIRTLAPNGFIATQDHVIIRIIGDISSATE HHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCHHHH LNESSALPRMSTKILKSELRAEAASLITLCLRSKSTRSGRWHDVEGSDLIQLRMTCFPLL CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHEEEE MDGEGEDHVLFSIEERESQPRTVATEDMNIDDRMVYVRQIEDEIASTREALQQTIEELQT ECCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC SNEELQSVNEEMQATNEELQATNEELETSNEELQATNEELITVNEEMQINSTELQQLSIE CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEEECCCCCCCHHHHHHHHHH LAAVLEATPYPTLLVDPALQIRHASAAAIDHFGLSELPPNGTHIGACTDGAGLAHLVSKV HHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHHHHHHHH AECFRTRESSTLEVNDETWSKTLTFTPIQKGVTQETIGVTVTVL HHHHHCCCCCEEEECCCHHCCEEEECHHHHCCCCHHCCEEEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Ca2+; Mg2+ [C]
Kcat value (1/min): 10 [C]
Specific activity: NA
Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]
Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]
Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade
General reaction: Methyl group transfer [C]
Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190 [H]