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The map label for this gene is cobB [H]

Identifier: 99082059

GI number: 99082059

Start: 2330775

End: 2332091

Strand: Direct

Name: cobB [H]

Synonym: TM1040_2219

Alternate gene names: 99082059

Gene position: 2330775-2332091 (Clockwise)

Preceding gene: 99082058

Following gene: 99082060

Centisome position: 72.82

GC content: 65.53

Gene sequence:

>1317_bases
ATGATCCCCTTCCCCAAAGGCCTGATGATCTCCGCGCCCTCCTCCGGCACCGGCAAGACCACTGTCATGCTGGGCCTTCT
GCGCGCGCTCAAGGACGATGGCCTGACCGTCCAGCCGTTCAAAAGCGGGCCGGATTATATCGACCCGGCCTTCCACCTTG
CTGCCGCCAGACGGGCGTCCTTCAACCTTGATACATGGGCCATGGACGACACGCTGCTAGACGCTGTCACCACCCAAGCC
CAAGGCGCGGGCATCTGCGTCGGCGAAGGGTCGATGGGGCTCTTCGACGGAGTCGCCACCCGCGGTCAGTCCGGCTTTGG
CTCCTCCGCGGAAACCGCGCTCAGGATGGGCTGGCCGGTGGTGCTGGTGATTGATGTGGGCGGTCAGGCGCAGTCGGCGG
CGGCCACCGCGCTGGGGTTCAAGATGTATAACCCCGATCTGCCCTTTGCCGGAGTGATCTTGAACCGGGTCGCGAGTCCC
CGTCACGACCGGCTTACCCGGCTTGGGATGGAGCGGGCGGGCGTCAAGGTGCTGGGCTCCCTGCCGAGACGCGGCGATCT
GGCTCTGCCCGAACGCCATCTCGGCCTCATTCAGGCGGTTGAACACCCCGATCTTGAGGCCGCAATCGCCGGCTATGCGG
CCTTCTTGCGCGAAAACGTCGACATTGACGCGATCCGGGCGGTGGCCAGCTCCGCCGCAGCACCGGCCCCTCGCGCCCTG
CCGCGTCCGCCCGCACAACGCATCGCATTGGCGCGCGATGCGGCGTTTTCCTTTACCTATCCGCATCTTCTCGAGGGTTG
GCGCGCCGCCGGAGCAGAGATCCTGCCCTTCTCGCCGCTCAACAATGAAGCCCCCGCCAAGGACGCCGATCTGGTCTGGC
TGCCCGGGGGCTATCCCGAGTTGCACGGCTCCACCCTCGCCGCAGCCGACATCTGGCGCAAAGGCATGGCAGAACACGCA
AAAACCAAACCGGTGCATGGCGAGTGCGGCGGCTACATGGCCCTTGGCGAGGCCTTGATCGACAAGGACGGCACGCGGCA
CCAGATGGCGGGGCTCCTTGGGCTTGTGACATCCTATGAAAAGCGCAAGTTCCACCTCGGGTATCGCCGCGCAGCGCTAG
AAGCGCCGATGCCGGGCTTTGACAGCGGCGCGTTTCTGCGCGGACATGAGTTCCATTATTCGACCATCCTCGACGAGCCA
GACGCCCCACTGGCCCGGGTGATGGACGCCGACGGCAACCCGGTGCCCGAGACCGGCTCCGTCAAAGGCCATGTCACCGG
TACGTTCTTTCACCTCATCACCGGAGAGCAGCCATGA

Upstream 100 bases:

>100_bases
ATCGCATGATGTGGGCCACCAAGGACAGCGCCGGAGCCTGGGTGCGGGCGCTGACCGGCTCGACGACCGGGCAAGAGATC
TCAGGACATGAGGTAACGAA

Downstream 100 bases:

>100_bases
GCGGGTTTGTCAGTTTTGTGTCCTCCGGGCCGGGCGATCCCGAGCTTTTGACCCTCAAGGCGGTCAAGCGGCTGGAGGCG
GCTGACGCGGTGCTGTTTGA

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MIPFPKGLMISAPSSGTGKTTVMLGLLRALKDDGLTVQPFKSGPDYIDPAFHLAAARRASFNLDTWAMDDTLLDAVTTQA
QGAGICVGEGSMGLFDGVATRGQSGFGSSAETALRMGWPVVLVIDVGGQAQSAAATALGFKMYNPDLPFAGVILNRVASP
RHDRLTRLGMERAGVKVLGSLPRRGDLALPERHLGLIQAVEHPDLEAAIAGYAAFLRENVDIDAIRAVASSAAAPAPRAL
PRPPAQRIALARDAAFSFTYPHLLEGWRAAGAEILPFSPLNNEAPAKDADLVWLPGGYPELHGSTLAAADIWRKGMAEHA
KTKPVHGECGGYMALGEALIDKDGTRHQMAGLLGLVTSYEKRKFHLGYRRAALEAPMPGFDSGAFLRGHEFHYSTILDEP
DAPLARVMDADGNPVPETGSVKGHVTGTFFHLITGEQP

Sequences:

>Translated_438_residues
MIPFPKGLMISAPSSGTGKTTVMLGLLRALKDDGLTVQPFKSGPDYIDPAFHLAAARRASFNLDTWAMDDTLLDAVTTQA
QGAGICVGEGSMGLFDGVATRGQSGFGSSAETALRMGWPVVLVIDVGGQAQSAAATALGFKMYNPDLPFAGVILNRVASP
RHDRLTRLGMERAGVKVLGSLPRRGDLALPERHLGLIQAVEHPDLEAAIAGYAAFLRENVDIDAIRAVASSAAAPAPRAL
PRPPAQRIALARDAAFSFTYPHLLEGWRAAGAEILPFSPLNNEAPAKDADLVWLPGGYPELHGSTLAAADIWRKGMAEHA
KTKPVHGECGGYMALGEALIDKDGTRHQMAGLLGLVTSYEKRKFHLGYRRAALEAPMPGFDSGAFLRGHEFHYSTILDEP
DAPLARVMDADGNPVPETGSVKGHVTGTFFHLITGEQP
>Mature_438_residues
MIPFPKGLMISAPSSGTGKTTVMLGLLRALKDDGLTVQPFKSGPDYIDPAFHLAAARRASFNLDTWAMDDTLLDAVTTQA
QGAGICVGEGSMGLFDGVATRGQSGFGSSAETALRMGWPVVLVIDVGGQAQSAAATALGFKMYNPDLPFAGVILNRVASP
RHDRLTRLGMERAGVKVLGSLPRRGDLALPERHLGLIQAVEHPDLEAAIAGYAAFLRENVDIDAIRAVASSAAAPAPRAL
PRPPAQRIALARDAAFSFTYPHLLEGWRAAGAEILPFSPLNNEAPAKDADLVWLPGGYPELHGSTLAAADIWRKGMAEHA
KTKPVHGECGGYMALGEALIDKDGTRHQMAGLLGLVTSYEKRKFHLGYRRAALEAPMPGFDSGAFLRGHEFHYSTILDEP
DAPLARVMDADGNPVPETGSVKGHVTGTFFHLITGEQP

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 46467; Mature: 46467

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFPKGLMISAPSSGTGKTTVMLGLLRALKDDGLTVQPFKSGPDYIDPAFHLAAARRAS
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
FNLDTWAMDDTLLDAVTTQAQGAGICVGEGSMGLFDGVATRGQSGFGSSAETALRMGWPV
CCCCCEECCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCE
VLVIDVGGQAQSAAATALGFKMYNPDLPFAGVILNRVASPRHDRLTRLGMERAGVKVLGS
EEEEECCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHC
LPRRGDLALPERHLGLIQAVEHPDLEAAIAGYAAFLRENVDIDAIRAVASSAAAPAPRAL
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
PRPPAQRIALARDAAFSFTYPHLLEGWRAAGAEILPFSPLNNEAPAKDADLVWLPGGYPE
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCC
LHGSTLAAADIWRKGMAEHAKTKPVHGECGGYMALGEALIDKDGTRHQMAGLLGLVTSYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRKFHLGYRRAALEAPMPGFDSGAFLRGHEFHYSTILDEPDAPLARVMDADGNPVPETGS
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEHHHCCCCCCCHHHHCCCCCCCCCCCCC
VKGHVTGTFFHLITGEQP
CCEEEEEEEEEEECCCCC
>Mature Secondary Structure
MIPFPKGLMISAPSSGTGKTTVMLGLLRALKDDGLTVQPFKSGPDYIDPAFHLAAARRAS
CCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCC
FNLDTWAMDDTLLDAVTTQAQGAGICVGEGSMGLFDGVATRGQSGFGSSAETALRMGWPV
CCCCCEECCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCE
VLVIDVGGQAQSAAATALGFKMYNPDLPFAGVILNRVASPRHDRLTRLGMERAGVKVLGS
EEEEECCCCCHHHHHHHHEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHC
LPRRGDLALPERHLGLIQAVEHPDLEAAIAGYAAFLRENVDIDAIRAVASSAAAPAPRAL
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
PRPPAQRIALARDAAFSFTYPHLLEGWRAAGAEILPFSPLNNEAPAKDADLVWLPGGYPE
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCC
LHGSTLAAADIWRKGMAEHAKTKPVHGECGGYMALGEALIDKDGTRHQMAGLLGLVTSYE
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRKFHLGYRRAALEAPMPGFDSGAFLRGHEFHYSTILDEPDAPLARVMDADGNPVPETGS
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEHHHCCCCCCCHHHHCCCCCCCCCCCCC
VKGHVTGTFFHLITGEQP
CCEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]