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The map label for this gene is pleD [H]

Identifier: 99082014

GI number: 99082014

Start: 2286545

End: 2287948

Strand: Direct

Name: pleD [H]

Synonym: TM1040_2174

Alternate gene names: 99082014

Gene position: 2286545-2287948 (Clockwise)

Preceding gene: 99082006

Following gene: 99082019

Centisome position: 71.43

GC content: 63.46

Gene sequence:

>1404_bases
GTGCAAGGCACGATCCTAGTCATAGATGGGGTATCCACCAATCGCATCATCTTGAAGGTGCAACTGACAGCAGCCTGTTT
CCAGGTGCTGCAGGCCGACAGCCTGTCGGCCGCGATGGCCCTGATGAAAGACAACCGCCCGGATCTCGTCCTCACTGCAA
TGACCCTGCCGGACGGCGATGCCGCGGATGTCAAACGCGCCCTGGACCGGGTGCGCCATGGCGTTGGGGTGCCGGTGATT
GCGCTCTGCCCGCAAAACGATCGCGGCACCCGCATCATGGCGCTGTCGGCGGGGATCGACGAGGTGCTGCCGCTTCCGGT
GGACGACATGATCCTGCAGGCGCGCATCCGCAGCCTGTTGCGCATGCGCATGCAAGACGACGAACTGCACCTGCGCGAAG
AGGGCGTTGCCGCCTTCTCCGTACCGCTGAAGGACACCACCGCCGTGCAGATCACACAATCGGATGCGACTGTCGCATTG
GTTTGTCATGATCTGCAGACGGCGCAAAAGTGGAAATCCCGCCTCAGCGCCAGCGTGGACTTCCGTCTGAAAGCTTACAA
TGTCGACGCGATCCAGGATCTGATGCGCACGCCGGTGCCGGATGTGATCGTGATCGAGCTTAACGAACACTCGGCTGGCA
TGGCGCTACGTCTGTTGGCGGATCTGCGTGCCCGGATCAGCACCCGCGACAGTGTGGTGATCGCGGTGCCGAACCCCGCC
GATGCCAGCATCGCGGCAGAGGCGCTGGACCGGGGTGCGCATGACGTCTTGCACTGCGGCTTTCAGGTGGATGAACTGGC
CCTACGGATCACCGCGCAGCTTGCACATCAGCAGCGCGCCGAAATGATCCGCAACACCCTGCGTGATGGCTATCGTGCGG
CGATGCGTGATCCGATGACCGGGCTCTACAATCGCCGCTACGCACGCCCGTTCCTCGAACAGGTGACCCAAGACGCCCAA
TCTGCGGGATCAAATTTCGCCGTGATGGTTGCGGATCTGGACCATTTCAAGCGCATCAACGATGCCTATGGCCACCCGGT
GGGCGATGCGGTGCTGATCGAAGCCGCGCGTCGCCTTCAAGCGGAGTTGCGTGGTTGCGACCTGCTGGCGCGGATCGGTG
GGGAGGAGTTCATGCTGGTGCTGCCGGATCTGCACCCCGACGAGGCAAGCACCGTGGCCAACCGACTGTGCCGCGCGATC
AGCGGCAGCCCCTTTGTGGTCAAAGGCTGCGCTCAGCCTCTCGAGGTTACAATCAGCGTCGGCGCCGTCGTCGCGCAGCA
TGGCACGCAGCCGTTGAGCGCCGATGATCTCTTTGTGGCGGCGGACCGCACGCTCTATGAGGCCAAGCACGCGGGCCGTA
ACCAGGCCGCGATCAAGGGGCTCACACAGAGCGCCGCCGCCTGA

Upstream 100 bases:

>100_bases
ACATTTTTAAGTCAATTTGCACTACCCCCGAAAGGGTTTGTTAACCAGTCTCAACAACACTCAAACACAAGCAAAAGCCG
GGAATGAAGGTGTTTTCAAC

Downstream 100 bases:

>100_bases
TCCCTCTGCATCCATAAGATCTGCAGATCACGCGTCCTTCCGGGATTATGGACGATCTTCGCGTGGCTTGCGAAACCGTG
CAATGTGGTCCCTTACCTTT

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MQGTILVIDGVSTNRIILKVQLTAACFQVLQADSLSAAMALMKDNRPDLVLTAMTLPDGDAADVKRALDRVRHGVGVPVI
ALCPQNDRGTRIMALSAGIDEVLPLPVDDMILQARIRSLLRMRMQDDELHLREEGVAAFSVPLKDTTAVQITQSDATVAL
VCHDLQTAQKWKSRLSASVDFRLKAYNVDAIQDLMRTPVPDVIVIELNEHSAGMALRLLADLRARISTRDSVVIAVPNPA
DASIAAEALDRGAHDVLHCGFQVDELALRITAQLAHQQRAEMIRNTLRDGYRAAMRDPMTGLYNRRYARPFLEQVTQDAQ
SAGSNFAVMVADLDHFKRINDAYGHPVGDAVLIEAARRLQAELRGCDLLARIGGEEFMLVLPDLHPDEASTVANRLCRAI
SGSPFVVKGCAQPLEVTISVGAVVAQHGTQPLSADDLFVAADRTLYEAKHAGRNQAAIKGLTQSAAA

Sequences:

>Translated_467_residues
MQGTILVIDGVSTNRIILKVQLTAACFQVLQADSLSAAMALMKDNRPDLVLTAMTLPDGDAADVKRALDRVRHGVGVPVI
ALCPQNDRGTRIMALSAGIDEVLPLPVDDMILQARIRSLLRMRMQDDELHLREEGVAAFSVPLKDTTAVQITQSDATVAL
VCHDLQTAQKWKSRLSASVDFRLKAYNVDAIQDLMRTPVPDVIVIELNEHSAGMALRLLADLRARISTRDSVVIAVPNPA
DASIAAEALDRGAHDVLHCGFQVDELALRITAQLAHQQRAEMIRNTLRDGYRAAMRDPMTGLYNRRYARPFLEQVTQDAQ
SAGSNFAVMVADLDHFKRINDAYGHPVGDAVLIEAARRLQAELRGCDLLARIGGEEFMLVLPDLHPDEASTVANRLCRAI
SGSPFVVKGCAQPLEVTISVGAVVAQHGTQPLSADDLFVAADRTLYEAKHAGRNQAAIKGLTQSAAA
>Mature_467_residues
MQGTILVIDGVSTNRIILKVQLTAACFQVLQADSLSAAMALMKDNRPDLVLTAMTLPDGDAADVKRALDRVRHGVGVPVI
ALCPQNDRGTRIMALSAGIDEVLPLPVDDMILQARIRSLLRMRMQDDELHLREEGVAAFSVPLKDTTAVQITQSDATVAL
VCHDLQTAQKWKSRLSASVDFRLKAYNVDAIQDLMRTPVPDVIVIELNEHSAGMALRLLADLRARISTRDSVVIAVPNPA
DASIAAEALDRGAHDVLHCGFQVDELALRITAQLAHQQRAEMIRNTLRDGYRAAMRDPMTGLYNRRYARPFLEQVTQDAQ
SAGSNFAVMVADLDHFKRINDAYGHPVGDAVLIEAARRLQAELRGCDLLARIGGEEFMLVLPDLHPDEASTVANRLCRAI
SGSPFVVKGCAQPLEVTISVGAVVAQHGTQPLSADDLFVAADRTLYEAKHAGRNQAAIKGLTQSAAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=197, Percent_Identity=37.0558375634518, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1786584, Length=163, Percent_Identity=33.1288343558282, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI87081881, Length=193, Percent_Identity=36.7875647668394, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=38.5542168674699, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=33.5403726708075, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=36.5853658536585, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693134, Length=197, Percent_Identity=27.9187817258883, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=31.25, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=31.0126582278481, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=211, Percent_Identity=27.9620853080569, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI87081977, Length=158, Percent_Identity=31.0126582278481, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50659; Mature: 50659

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGTILVIDGVSTNRIILKVQLTAACFQVLQADSLSAAMALMKDNRPDLVLTAMTLPDGD
CCCEEEEEECCCCCEEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCC
AADVKRALDRVRHGVGVPVIALCPQNDRGTRIMALSAGIDEVLPLPVDDMILQARIRSLL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHH
RMRMQDDELHLREEGVAAFSVPLKDTTAVQITQSDATVALVCHDLQTAQKWKSRLSASVD
HHHCCCCHHHHHHCCCEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCEE
FRLKAYNVDAIQDLMRTPVPDVIVIELNEHSAGMALRLLADLRARISTRDSVVIAVPNPA
EEEEEECHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
DASIAAEALDRGAHDVLHCGFQVDELALRITAQLAHQQRAEMIRNTLRDGYRAAMRDPMT
CHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLYNRRYARPFLEQVTQDAQSAGSNFAVMVADLDHFKRINDAYGHPVGDAVLIEAARRLQ
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AELRGCDLLARIGGEEFMLVLPDLHPDEASTVANRLCRAISGSPFVVKGCAQPLEVTISV
HHHHCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEH
GAVVAQHGTQPLSADDLFVAADRTLYEAKHAGRNQAAIKGLTQSAAA
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MQGTILVIDGVSTNRIILKVQLTAACFQVLQADSLSAAMALMKDNRPDLVLTAMTLPDGD
CCCEEEEEECCCCCEEEEEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCC
AADVKRALDRVRHGVGVPVIALCPQNDRGTRIMALSAGIDEVLPLPVDDMILQARIRSLL
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEEECCCHHHCCCCHHHHHHHHHHHHHH
RMRMQDDELHLREEGVAAFSVPLKDTTAVQITQSDATVALVCHDLQTAQKWKSRLSASVD
HHHCCCCHHHHHHCCCEEEEECCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHCCCCEE
FRLKAYNVDAIQDLMRTPVPDVIVIELNEHSAGMALRLLADLRARISTRDSVVIAVPNPA
EEEEEECHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCC
DASIAAEALDRGAHDVLHCGFQVDELALRITAQLAHQQRAEMIRNTLRDGYRAAMRDPMT
CHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
GLYNRRYARPFLEQVTQDAQSAGSNFAVMVADLDHFKRINDAYGHPVGDAVLIEAARRLQ
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AELRGCDLLARIGGEEFMLVLPDLHPDEASTVANRLCRAISGSPFVVKGCAQPLEVTISV
HHHHCCHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEH
GAVVAQHGTQPLSADDLFVAADRTLYEAKHAGRNQAAIKGLTQSAAA
HHHHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA