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The map label for this gene is kipI [H]

Identifier: 99081897

GI number: 99081897

Start: 2160788

End: 2161558

Strand: Reverse

Name: kipI [H]

Synonym: TM1040_2057

Alternate gene names: 99081897

Gene position: 2161558-2160788 (Counterclockwise)

Preceding gene: 99081901

Following gene: 99081896

Centisome position: 67.53

GC content: 67.06

Gene sequence:

>771_bases
ATGACAAAAAGCCCCATACGTGTTGAGCCCCCCGTCGGGGATACGCAATGGCCCGAAGTCGCGACAGCCGGCTTTGACGG
CGTGGTGGTGCGCTTTGCCGACCGGCTGAGTGAGCCCGCAAATCGCGCAGCACTTGCGTTTCGTGCGGCGGTGGATGTGG
CCGGTTGGGCTGCGGTTGAAGAGACATCGACCTCCTTGGTGTCCACCTACCTGCGTTTTGACCCTCTGGGGACGTCACAT
GCCGAGATGATTGCCCAGGTCGCGGCGCTGCTGGAGACCCGCGACTGGTACGCCGCCGCCTTGCCGCAGGGGCGCAAGCT
CTGGCGGGTGCCGACGGTGTTTGGCACCGAACTCGCCCCCCAGCTCGAAGAGGCTGCGGCGCGGGCCGGCCTCAGCGCAG
GTGAGGCGGTCACGCAGATTTCACAGACCCGGCTTCGGGTGCAGACCATCGGCTTTGCCCCCGGCCAGCCTTACCTGGGC
GAGTTGCCGCCCGCCTTTGACATCCCGCGCCAGACCGAGCTCACCCCCGAAGTCCCCGAGGGGGCCGTGGTCGTGGCGAT
CCGCCAGATCGTGCTGTTTTCTGTCACGACCCCAACCGGCTGGCGCCACATCGGTCAGACCCGCTTTCGCCTCTTTCGCC
CCGACGCGCCAGAGCCCTTTGTGCTGCGTCCGGGCGATGAAGTGCTGTTTGAGCCCATCTCGCCCGAACGCTACGCCGCG
CTCGAGCGCGCGGGAGTGGATGGTGGCGCCACCTCCGAGGTGCTGACATGA

Upstream 100 bases:

>100_bases
GCTTCGCGCAGGCACTTGGCGAGAGGCTTCCCGACAAAAGCCCGGCCGCTACCGACATTTGGGTCTGGTCTTTCCCGGCA
ACCGCTGGCAGTCATATGCC

Downstream 100 bases:

>100_bases
GCGTGCGCCTTATTGTTCACCGCGCAGGACCGGGTCTGAGCATTCAGGATCTTGGCCGTAGCGGCTATCTCGCCTTTGGT
CTGTCGCGCGGTGGCGCGGC

Product: allophanate hydrolase subunit 1

Products: NA

Alternate protein names: Sporulation inhibitor kipI [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTKSPIRVEPPVGDTQWPEVATAGFDGVVVRFADRLSEPANRAALAFRAAVDVAGWAAVEETSTSLVSTYLRFDPLGTSH
AEMIAQVAALLETRDWYAAALPQGRKLWRVPTVFGTELAPQLEEAAARAGLSAGEAVTQISQTRLRVQTIGFAPGQPYLG
ELPPAFDIPRQTELTPEVPEGAVVVAIRQIVLFSVTTPTGWRHIGQTRFRLFRPDAPEPFVLRPGDEVLFEPISPERYAA
LERAGVDGGATSEVLT

Sequences:

>Translated_256_residues
MTKSPIRVEPPVGDTQWPEVATAGFDGVVVRFADRLSEPANRAALAFRAAVDVAGWAAVEETSTSLVSTYLRFDPLGTSH
AEMIAQVAALLETRDWYAAALPQGRKLWRVPTVFGTELAPQLEEAAARAGLSAGEAVTQISQTRLRVQTIGFAPGQPYLG
ELPPAFDIPRQTELTPEVPEGAVVVAIRQIVLFSVTTPTGWRHIGQTRFRLFRPDAPEPFVLRPGDEVLFEPISPERYAA
LERAGVDGGATSEVLT
>Mature_255_residues
TKSPIRVEPPVGDTQWPEVATAGFDGVVVRFADRLSEPANRAALAFRAAVDVAGWAAVEETSTSLVSTYLRFDPLGTSHA
EMIAQVAALLETRDWYAAALPQGRKLWRVPTVFGTELAPQLEEAAARAGLSAGEAVTQISQTRLRVQTIGFAPGQPYLGE
LPPAFDIPRQTELTPEVPEGAVVVAIRQIVLFSVTTPTGWRHIGQTRFRLFRPDAPEPFVLRPGDEVLFEPISPERYAAL
ERAGVDGGATSEVLT

Specific function: Is a potent inhibitor of the autophosphorylation reaction of kinase A and its reverse reaction, but does not inhibit phosphate transfer to the Spo0F response regulator once kinase A is phosphorylated. KipI is an inhibitor of the catalytic domain of kinase

COG id: COG2049

COG function: function code E; Allophanate hydrolase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786929, Length=146, Percent_Identity=37.6712328767123, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003833
- InterPro:   IPR020899
- InterPro:   IPR010016
- InterPro:   IPR002130 [H]

Pfam domain/function: PF02682 AHS1 [H]

EC number: NA

Molecular weight: Translated: 27700; Mature: 27568

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSPIRVEPPVGDTQWPEVATAGFDGVVVRFADRLSEPANRAALAFRAAVDVAGWAAVE
CCCCCCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ETSTSLVSTYLRFDPLGTSHAEMIAQVAALLETRDWYAAALPQGRKLWRVPTVFGTELAP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCHHCCHHHH
QLEEAAARAGLSAGEAVTQISQTRLRVQTIGFAPGQPYLGELPPAFDIPRQTELTPEVPE
HHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAVVVAIRQIVLFSVTTPTGWRHIGQTRFRLFRPDAPEPFVLRPGDEVLFEPISPERYAA
CHHHHHHHHHHHEECCCCCCHHHHCHHHHEEECCCCCCCEEECCCCHHEECCCCCHHHHH
LERAGVDGGATSEVLT
HHHCCCCCCCCHHCCC
>Mature Secondary Structure 
TKSPIRVEPPVGDTQWPEVATAGFDGVVVRFADRLSEPANRAALAFRAAVDVAGWAAVE
CCCCCEECCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
ETSTSLVSTYLRFDPLGTSHAEMIAQVAALLETRDWYAAALPQGRKLWRVPTVFGTELAP
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEECCCHHCCHHHH
QLEEAAARAGLSAGEAVTQISQTRLRVQTIGFAPGQPYLGELPPAFDIPRQTELTPEVPE
HHHHHHHHCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAVVVAIRQIVLFSVTTPTGWRHIGQTRFRLFRPDAPEPFVLRPGDEVLFEPISPERYAA
CHHHHHHHHHHHEECCCCCCHHHHCHHHHEEECCCCCCCEEECCCCHHEECCCCCHHHHH
LERAGVDGGATSEVLT
HHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377; 10568751 [H]