Click here to switch to the map view.

The map label for this gene is cobQ

Identifier: 99081829

GI number: 99081829

Start: 2092984

End: 2094432

Strand: Direct

Name: cobQ

Synonym: TM1040_1989

Alternate gene names: 99081829

Gene position: 2092984-2094432 (Clockwise)

Preceding gene: 99081828

Following gene: 99081834

Centisome position: 65.39

GC content: 64.73

Gene sequence:

>1449_bases
ATGATCCAAGGCACCGGCAGCAATGTCGGAAAATCCATGCTGGTGGCAGGGCTAGCACGGGCCTTGCGCAAACGCGGCCT
CTCGGTGGCGCCCTTCAAACCGCAGAACATGTCCAACAACGCCGCCGTCACCTCGGACGGGGGGGAGATCGGCCGCGCCC
AGGCCCTGCAGGCCCGCGCGGCGGGGCTTGCGCCGCATACGGATATGAACCCGGTGCTCCTGAAGCCCGAAACCGATACC
GGCGCGCAGGTCATCGTGCAGGGCAAGCGGCGCGGCACCCGCGCGGCGGGGTCGTTTATGCGCGACAAGGCGGGCCTTCT
GGAAGCCACGCTCGAGAGCTTTCACCGCCTCGCAGCGCAGCATGACATTGTCCTCATCGAGGGCGCAGGCTCTCCGGCAG
AAACCAATCTGCGCAAGGGCGACATCGCCAATATGGGCTTTGCCGAAGCTGCAGGCGTACCTGTCTTGCTGGTGGGCGAC
ATCCATCGGGGCGGGGTGATCGCGCAGATCGTTGGCACCCATACGGTGTTGGAGCCAAGCGACCGCGCGCGGATCAAAGC
CTTCGCCGTCAATCGCTTCCGGGGCGACCTTAGCCTTTTTGATGGCGGGCGGGATGACATTGCGCGCTGGACGGGCTGGC
CTTCGCTGGGGGTGGTGCCATGGTTCTGGGATGCGTGGAAACTGCCGGCCGAGGATATGATGGACATCGCCTCCCACAAG
GGCGGCGCTTGCAAGGTGGTGGTGCCGCAGCTTGAACGCATGGCGAATTTCGACGACCTCGACCCGCTTGCAGCAGAACC
TGCGGTGACGGTCGAGATCGTGCCCCCCGGGCGCGCCCTGCCCGGTGATGCGGATCTGGTGCTGATCCCCGGCTCCAAAT
CCACTATCGGCGATCTGGCCTATCTGCGCACGCAGGGCTGGGACATCGACATCCTCGCTCATCACCGGCGCGGCGGACAT
GTGCTCGGGCTTTGTGGCGGCTATCAGATGCTCGGCCAGAGTATCGACGATCCCGAAGGTGTCGATGGCCATCCCGGCAA
AGTCGCGGGGCTTGGCCTCTTGGATGTCCACACTGTTATGGCCGGAGACAAGCGCGTCACCCTGAGCGCGGCGCGCACAC
TCGAGGGGGATCTGCCTGTTTCTGGCTATGAGATCCACATGGGCCGCACCACGGGGCCGGATTGCGCGCGGGCCTGGCTC
GCGCTCGAAGGCCGCGCGGAGGGGGCGACCTCTGCCGATGGGCGTGTGCGCGGCTCTTATCTGCACGGGCTTTTTACATC
GGACGCGTTTCGGGCACAGTTCCTCTCCGACCTCGGACACCAGTCCGATCTGGACTATGACGCCGGGGTCGAGGCGACGC
TTGATGAGCTTGCAGCCCATCTTGAACAATATATGGATGTGGAAGGCCTGCTCGAACTGGCCGAACCCATTCCTGTGCCT
GAATCCTGA

Upstream 100 bases:

>100_bases
CCTTGGGCGACGCTTGCGCGATTGACCTTTGGACGGCGGCGTTGCAACCCTGCGCGAGACACCTCAGCAAACGAGACCAG
CAATGACCCCAAAGGCCCTC

Downstream 100 bases:

>100_bases
GATGCCCAGAGCGACTGACGCCAGCACGGCCCAACCAAACGACGGGTTGTGAAAAACAAGCCGGACGGGTGAAGGATGAG
GAGTGAAGGATACTGGCGAA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIQGTGSNVGKSMLVAGLARALRKRGLSVAPFKPQNMSNNAAVTSDGGEIGRAQALQARAAGLAPHTDMNPVLLKPETDT
GAQVIVQGKRRGTRAAGSFMRDKAGLLEATLESFHRLAAQHDIVLIEGAGSPAETNLRKGDIANMGFAEAAGVPVLLVGD
IHRGGVIAQIVGTHTVLEPSDRARIKAFAVNRFRGDLSLFDGGRDDIARWTGWPSLGVVPWFWDAWKLPAEDMMDIASHK
GGACKVVVPQLERMANFDDLDPLAAEPAVTVEIVPPGRALPGDADLVLIPGSKSTIGDLAYLRTQGWDIDILAHHRRGGH
VLGLCGGYQMLGQSIDDPEGVDGHPGKVAGLGLLDVHTVMAGDKRVTLSAARTLEGDLPVSGYEIHMGRTTGPDCARAWL
ALEGRAEGATSADGRVRGSYLHGLFTSDAFRAQFLSDLGHQSDLDYDAGVEATLDELAAHLEQYMDVEGLLELAEPIPVP
ES

Sequences:

>Translated_482_residues
MIQGTGSNVGKSMLVAGLARALRKRGLSVAPFKPQNMSNNAAVTSDGGEIGRAQALQARAAGLAPHTDMNPVLLKPETDT
GAQVIVQGKRRGTRAAGSFMRDKAGLLEATLESFHRLAAQHDIVLIEGAGSPAETNLRKGDIANMGFAEAAGVPVLLVGD
IHRGGVIAQIVGTHTVLEPSDRARIKAFAVNRFRGDLSLFDGGRDDIARWTGWPSLGVVPWFWDAWKLPAEDMMDIASHK
GGACKVVVPQLERMANFDDLDPLAAEPAVTVEIVPPGRALPGDADLVLIPGSKSTIGDLAYLRTQGWDIDILAHHRRGGH
VLGLCGGYQMLGQSIDDPEGVDGHPGKVAGLGLLDVHTVMAGDKRVTLSAARTLEGDLPVSGYEIHMGRTTGPDCARAWL
ALEGRAEGATSADGRVRGSYLHGLFTSDAFRAQFLSDLGHQSDLDYDAGVEATLDELAAHLEQYMDVEGLLELAEPIPVP
ES
>Mature_482_residues
MIQGTGSNVGKSMLVAGLARALRKRGLSVAPFKPQNMSNNAAVTSDGGEIGRAQALQARAAGLAPHTDMNPVLLKPETDT
GAQVIVQGKRRGTRAAGSFMRDKAGLLEATLESFHRLAAQHDIVLIEGAGSPAETNLRKGDIANMGFAEAAGVPVLLVGD
IHRGGVIAQIVGTHTVLEPSDRARIKAFAVNRFRGDLSLFDGGRDDIARWTGWPSLGVVPWFWDAWKLPAEDMMDIASHK
GGACKVVVPQLERMANFDDLDPLAAEPAVTVEIVPPGRALPGDADLVLIPGSKSTIGDLAYLRTQGWDIDILAHHRRGGH
VLGLCGGYQMLGQSIDDPEGVDGHPGKVAGLGLLDVHTVMAGDKRVTLSAARTLEGDLPVSGYEIHMGRTTGPDCARAWL
ALEGRAEGATSADGRVRGSYLHGLFTSDAFRAQFLSDLGHQSDLDYDAGVEATLDELAAHLEQYMDVEGLLELAEPIPVP
ES

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_SILST (Q1GF45)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613983.1
- ProteinModelPortal:   Q1GF45
- STRING:   Q1GF45
- GeneID:   4077173
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1989
- NMPDR:   fig|292414.1.peg.371
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   SSP292414:TM1040_1989-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51028; Mature: 51028

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 325-325 ACT_SITE 422-422

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQGTGSNVGKSMLVAGLARALRKRGLSVAPFKPQNMSNNAAVTSDGGEIGRAQALQARA
CCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
AGLAPHTDMNPVLLKPETDTGAQVIVQGKRRGTRAAGSFMRDKAGLLEATLESFHRLAAQ
HCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HDIVLIEGAGSPAETNLRKGDIANMGFAEAAGVPVLLVGDIHRGGVIAQIVGTHTVLEPS
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCEEEEEECCCEEECCC
DRARIKAFAVNRFRGDLSLFDGGRDDIARWTGWPSLGVVPWFWDAWKLPAEDMMDIASHK
CCCEEEEEEEEHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GGACKVVVPQLERMANFDDLDPLAAEPAVTVEIVPPGRALPGDADLVLIPGSKSTIGDLA
CCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHH
YLRTQGWDIDILAHHRRGGHVLGLCGGYQMLGQSIDDPEGVDGHPGKVAGLGLLDVHTVM
HHHHCCCEEEEEEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
AGDKRVTLSAARTLEGDLPVSGYEIHMGRTTGPDCARAWLALEGRAEGATSADGRVRGSY
CCCCEEEEEEHHHCCCCCCCCCEEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCEEHHH
LHGLFTSDAFRAQFLSDLGHQSDLDYDAGVEATLDELAAHLEQYMDVEGLLELAEPIPVP
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
ES
CC
>Mature Secondary Structure
MIQGTGSNVGKSMLVAGLARALRKRGLSVAPFKPQNMSNNAAVTSDGGEIGRAQALQARA
CCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCEEECCCCCCCHHHHHHHHH
AGLAPHTDMNPVLLKPETDTGAQVIVQGKRRGTRAAGSFMRDKAGLLEATLESFHRLAAQ
HCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HDIVLIEGAGSPAETNLRKGDIANMGFAEAAGVPVLLVGDIHRGGVIAQIVGTHTVLEPS
CCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCEEEEEECCCEEECCC
DRARIKAFAVNRFRGDLSLFDGGRDDIARWTGWPSLGVVPWFWDAWKLPAEDMMDIASHK
CCCEEEEEEEEHHCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GGACKVVVPQLERMANFDDLDPLAAEPAVTVEIVPPGRALPGDADLVLIPGSKSTIGDLA
CCEEEEEHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCCHHHHH
YLRTQGWDIDILAHHRRGGHVLGLCGGYQMLGQSIDDPEGVDGHPGKVAGLGLLDVHTVM
HHHHCCCEEEEEEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEHHHHHHHH
AGDKRVTLSAARTLEGDLPVSGYEIHMGRTTGPDCARAWLALEGRAEGATSADGRVRGSY
CCCCEEEEEEHHHCCCCCCCCCEEEEECCCCCHHHHHHHHEEECCCCCCCCCCCCEEHHH
LHGLFTSDAFRAQFLSDLGHQSDLDYDAGVEATLDELAAHLEQYMDVEGLLELAEPIPVP
HHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCC
ES
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA