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The map label for this gene is yngF [H]

Identifier: 99081820

GI number: 99081820

Start: 2083971

End: 2084768

Strand: Reverse

Name: yngF [H]

Synonym: TM1040_1980

Alternate gene names: 99081820

Gene position: 2084768-2083971 (Counterclockwise)

Preceding gene: 99081823

Following gene: 99081819

Centisome position: 65.13

GC content: 65.04

Gene sequence:

>798_bases
ATGGCAGAGGAAAGCGCGCGTGTATCTGTCGAGATCCGCGACCATGTGGCCCATGTCACCCTGACCCGCGCCGACAAGAT
GAACGCGGTGGACAAACACATGCTGGCGGCCGTGATCGCCGCCGCCAAAGAGGTTGCCGCCTCTGATGCCCGTGCGGTGG
TGCTCTCTGGCGCGGGTAAGAGCTTTTGCGCGGGCCTGGATGTGATGAGCTTTGCCGCGATGGGGCAGATGCAGGACACC
GGCTGGATCATGGATCGCACCGAGGGGGAGGCCAATGACTTTCAGGCGGTGGCGATGATGTGGCGCGCGCTTGAGATCCC
GGTGATTGCGGCCTTGCAGGGGGCGACCTTTGGGGCGGGGCTGCAGATTGCGCTCGGCGCAGACATCCGTATTGCCGCGC
CCGATGCCAAGCTTGCGGTGATGGAGATGAAATGGGGGCTGGTCCCCGATATGGGCGGCATGGCGCTCTTGCCGCAGTTG
GTGCGCTCGGATGTGCTGCGTCTGCTGACCTACAGCGCGACCCCCATCGAGGCCACCCAAGCCGAGACCTGGGGACTGGT
GACGGAAGTGGCCGAGGACCCTCTGGCGCGCGCCGAGGCGCTTGCGGCAGAGATCGCAGGCAAGGGCCCGCGCGCCATTC
GCGCCGCCAAACGCCTGATCGCCTATGCCGAGAGCGGGGCCAGCACGGCGGATGTGCTGTTGCGCGAAAGCGAAGAGCAG
GCCGCCCTGATCGGCAAGCCAGAGCAGATGGAAATCGTCATGGCCAATATGCAAAAGCGCGCGCCCGTCTTCAAATAA

Upstream 100 bases:

>100_bases
GGATCCGGTCCGGCGTGCCCTCGCGTCAGGCTGGAAAGCACGCCCGAAGCGCGGCAAGCTGCACGCAACATTAAGACGAC
ACCCAGAGGGAGGAGCCGTG

Downstream 100 bases:

>100_bases
GACCAGCGCGCGGCCCCCGGGCAAAGCAGATCATGGGGAGGAGGAGATCTGATGCGTTTGAAACTCTTGCTGTCGCACCC
TTCGGTGCCAGTGATGCTCG

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MAEESARVSVEIRDHVAHVTLTRADKMNAVDKHMLAAVIAAAKEVAASDARAVVLSGAGKSFCAGLDVMSFAAMGQMQDT
GWIMDRTEGEANDFQAVAMMWRALEIPVIAALQGATFGAGLQIALGADIRIAAPDAKLAVMEMKWGLVPDMGGMALLPQL
VRSDVLRLLTYSATPIEATQAETWGLVTEVAEDPLARAEALAAEIAGKGPRAIRAAKRLIAYAESGASTADVLLRESEEQ
AALIGKPEQMEIVMANMQKRAPVFK

Sequences:

>Translated_265_residues
MAEESARVSVEIRDHVAHVTLTRADKMNAVDKHMLAAVIAAAKEVAASDARAVVLSGAGKSFCAGLDVMSFAAMGQMQDT
GWIMDRTEGEANDFQAVAMMWRALEIPVIAALQGATFGAGLQIALGADIRIAAPDAKLAVMEMKWGLVPDMGGMALLPQL
VRSDVLRLLTYSATPIEATQAETWGLVTEVAEDPLARAEALAAEIAGKGPRAIRAAKRLIAYAESGASTADVLLRESEEQ
AALIGKPEQMEIVMANMQKRAPVFK
>Mature_264_residues
AEESARVSVEIRDHVAHVTLTRADKMNAVDKHMLAAVIAAAKEVAASDARAVVLSGAGKSFCAGLDVMSFAAMGQMQDTG
WIMDRTEGEANDFQAVAMMWRALEIPVIAALQGATFGAGLQIALGADIRIAAPDAKLAVMEMKWGLVPDMGGMALLPQLV
RSDVLRLLTYSATPIEATQAETWGLVTEVAEDPLARAEALAAEIAGKGPRAIRAAKRLIAYAESGASTADVLLRESEEQA
ALIGKPEQMEIVMANMQKRAPVFK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=229, Percent_Identity=34.4978165938865, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=30.4812834224599, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=28.0155642023346, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787660, Length=255, Percent_Identity=31.7647058823529, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=28.5171102661597, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI221142681, Length=221, Percent_Identity=29.8642533936652, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=29.3814432989691, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=280, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=170, Percent_Identity=32.9411764705882, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=221, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=211, Percent_Identity=29.8578199052133, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=185, Percent_Identity=27.5675675675676, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=26.984126984127, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=158, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=32.5991189427313, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=26.3157894736842, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=25.7575757575758, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28087; Mature: 27956

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEESARVSVEIRDHVAHVTLTRADKMNAVDKHMLAAVIAAAKEVAASDARAVVLSGAGK
CCCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SFCAGLDVMSFAAMGQMQDTGWIMDRTEGEANDFQAVAMMWRALEIPVIAALQGATFGAG
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCCC
LQIALGADIRIAAPDAKLAVMEMKWGLVPDMGGMALLPQLVRSDVLRLLTYSATPIEATQ
EEEEECCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
AETWGLVTEVAEDPLARAEALAAEIAGKGPRAIRAAKRLIAYAESGASTADVLLRESEEQ
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCH
AALIGKPEQMEIVMANMQKRAPVFK
HHHCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AEESARVSVEIRDHVAHVTLTRADKMNAVDKHMLAAVIAAAKEVAASDARAVVLSGAGK
CCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCC
SFCAGLDVMSFAAMGQMQDTGWIMDRTEGEANDFQAVAMMWRALEIPVIAALQGATFGAG
HHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCHHEEECCCCCCCC
LQIALGADIRIAAPDAKLAVMEMKWGLVPDMGGMALLPQLVRSDVLRLLTYSATPIEATQ
EEEEECCCEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
AETWGLVTEVAEDPLARAEALAAEIAGKGPRAIRAAKRLIAYAESGASTADVLLRESEEQ
CHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCH
AALIGKPEQMEIVMANMQKRAPVFK
HHHCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]