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The map label for this gene is gatA

Identifier: 99081759

GI number: 99081759

Start: 2019347

End: 2020834

Strand: Reverse

Name: gatA

Synonym: TM1040_1918

Alternate gene names: 99081759

Gene position: 2020834-2019347 (Counterclockwise)

Preceding gene: 99081760

Following gene: 99081758

Centisome position: 63.13

GC content: 64.38

Gene sequence:

>1488_bases
ATGTCTGACCTGAACAAACTGGGCCTTGCAGAGGCCCGCGATCTGCTGCGCAAGGGTGACACCACCTCTGTGGAACTCAC
CGAGGCCTGCCTCAAGGCCATCGACGGCGCGGATGCGCTCAACGCGTTTGTGCATAAGACCCCCGACGTCGCTCTGGACC
GTGCGAAGGCGGCGGACGCGCGGATTGCCGAGGGCGATGCGCCCGCCATGTGCGGCCTGCCCATCGGCATGAAGGACCTC
TTCTGCACCAAAGGCGTCGACAGCCAGGCGGCGTCGAACATCCTCAAGGGCTTCAAGCCCGAATATGAATCCACCGTCTC
GCAGAAACTGCAGGACGCCGGTGCGGTGATGCTCGGCAAGCTCAATATGGACGAATTTGCCATGGGCTCGTCGAACGAGA
CCTCCGCCTATGGCAATGCCATCAGCCCATGGCGGCGCGAGGGGGACGAAACCCCGCTGACGCCCGGTGGCTCTTCTGGT
GGCTCGGCGGCGGCTGTGGCGGCAGACCTGTGCCTTGCCGCCACCGGCACCGACACCGGCGGCTCGATCCGCCAGCCTGC
GGCCTTCACCGGCACCGTTGGCATCAAGCCCACCTATGGGCGCTGCTCGCGCTGGGGCATCGTGGCCTTTGCCTCCTCGC
TCGATCAGGCTGGCCCGATGACCAAGAATGTGCGCGACGCCGCCATCATGCTCGAGGCCATGTGCGGCCATGACGCGAAG
GATTCGACCTCTGTGGACCTCGCGGTGCCGAACTTCGAGGCGATGCTGACCGGCGACATCAAAGGCAAGAAAATCGGCAT
CCCGCGCGAATACCGCATGGACGGTATGCCCGCCGAGATCGCCAAGCTCTGGGATGAGGGCGCCGCGATGCTCAAGGCCG
CGGGCGCTGAGATCGTAGACATCTCGCTGCCGCACACGAAATACGCGCTGCCCGCCTATTACGTGATTGCCCCGGCTGAG
GCCTCTTCGAACCTGGCGCGGTACGACGGTGTGCGCTACGGCCACCGCGCGACGCTCGCGCAGGGGGACGGCATCACCGA
GATGTACGAGAAAACTCGCGCCGAAGGCTTTGGCCACGAGGTGCAGCGCCGCGTGATGGTCGGCACCTATGTGCTGTCGG
CAGGGTTCTACGACGCCTATTACAACCGCGCGCGCAAGGTCCGCACCTTGATCAAGAAGGATTTCGAAGATGTCTTTGCT
GCGGGTGTGGACGCGATCCTGACCCCGGCGACGCCCTCCGCGGCTTTTGGCCTTGGCGAGATGAACGACGAAGATCCGGT
CAAGATGTACCTCAACGACGTCTTCACTGTCACCGTGAACCTAGCGGGCCTGCCCGGGATTTCGGTGCCAACCGGCGTCG
ACACGCAGGGGCTGCCGCTCGGTCTGCAATTGATCGGACGTCCATGGGACGAGGGCGATCTTCTGAATACCGCCTACGCG
CTGGAGCAAGCGGCCGGATTTGTGGCCAAGCCCGCCCAGTGGTGGTAA

Upstream 100 bases:

>100_bases
GGATGTGGTCACCGATGGCAACCAGCAGGACAAAGTGCTGGCCAATGCCCCAGATGCCCGCGAAGGCTTCTTTGCTGTGC
CCAAGGTTGTGGAGTAAGAC

Downstream 100 bases:

>100_bases
AAGGACCTCCAAAGCAAGACTGAGCAGACAGGTGGCAGCAATGCGAGTTCCAGCAATCTTGGTCGCGGTCAGCGCGATTG
CACTTGTGGCTTGTAGCCCG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MSDLNKLGLAEARDLLRKGDTTSVELTEACLKAIDGADALNAFVHKTPDVALDRAKAADARIAEGDAPAMCGLPIGMKDL
FCTKGVDSQAASNILKGFKPEYESTVSQKLQDAGAVMLGKLNMDEFAMGSSNETSAYGNAISPWRREGDETPLTPGGSSG
GSAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKNVRDAAIMLEAMCGHDAK
DSTSVDLAVPNFEAMLTGDIKGKKIGIPREYRMDGMPAEIAKLWDEGAAMLKAAGAEIVDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRYGHRATLAQGDGITEMYEKTRAEGFGHEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKKDFEDVFA
AGVDAILTPATPSAAFGLGEMNDEDPVKMYLNDVFTVTVNLAGLPGISVPTGVDTQGLPLGLQLIGRPWDEGDLLNTAYA
LEQAAGFVAKPAQWW

Sequences:

>Translated_495_residues
MSDLNKLGLAEARDLLRKGDTTSVELTEACLKAIDGADALNAFVHKTPDVALDRAKAADARIAEGDAPAMCGLPIGMKDL
FCTKGVDSQAASNILKGFKPEYESTVSQKLQDAGAVMLGKLNMDEFAMGSSNETSAYGNAISPWRREGDETPLTPGGSSG
GSAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKNVRDAAIMLEAMCGHDAK
DSTSVDLAVPNFEAMLTGDIKGKKIGIPREYRMDGMPAEIAKLWDEGAAMLKAAGAEIVDISLPHTKYALPAYYVIAPAE
ASSNLARYDGVRYGHRATLAQGDGITEMYEKTRAEGFGHEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKKDFEDVFA
AGVDAILTPATPSAAFGLGEMNDEDPVKMYLNDVFTVTVNLAGLPGISVPTGVDTQGLPLGLQLIGRPWDEGDLLNTAYA
LEQAAGFVAKPAQWW
>Mature_494_residues
SDLNKLGLAEARDLLRKGDTTSVELTEACLKAIDGADALNAFVHKTPDVALDRAKAADARIAEGDAPAMCGLPIGMKDLF
CTKGVDSQAASNILKGFKPEYESTVSQKLQDAGAVMLGKLNMDEFAMGSSNETSAYGNAISPWRREGDETPLTPGGSSGG
SAAAVAADLCLAATGTDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKNVRDAAIMLEAMCGHDAKD
STSVDLAVPNFEAMLTGDIKGKKIGIPREYRMDGMPAEIAKLWDEGAAMLKAAGAEIVDISLPHTKYALPAYYVIAPAEA
SSNLARYDGVRYGHRATLAQGDGITEMYEKTRAEGFGHEVQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKKDFEDVFAA
GVDAILTPATPSAAFGLGEMNDEDPVKMYLNDVFTVTVNLAGLPGISVPTGVDTQGLPLGLQLIGRPWDEGDLLNTAYAL
EQAAGFVAKPAQWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=41.8837675350701, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=24.1035856573705, Blast_Score=110, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=442, Percent_Identity=37.7828054298643, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=26.7068273092369, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17538252, Length=241, Percent_Identity=28.6307053941909, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=309, Percent_Identity=26.8608414239482, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=204, Percent_Identity=32.843137254902, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556264, Length=215, Percent_Identity=26.9767441860465, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=35.6481481481481, Blast_Score=251, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=462, Percent_Identity=25.974025974026, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=38.2716049382716, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=24.5472837022133, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=24.5472837022133, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=24.5472837022133, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=24.5472837022133, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161078093, Length=527, Percent_Identity=24.2884250474383, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644968, Length=264, Percent_Identity=31.4393939393939, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356731, Length=504, Percent_Identity=24.0079365079365, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45550774, Length=253, Percent_Identity=26.4822134387352, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648435, Length=253, Percent_Identity=26.4822134387352, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648437, Length=253, Percent_Identity=26.4822134387352, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_SILST (Q1GFB5)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613913.1
- ProteinModelPortal:   Q1GFB5
- SMR:   Q1GFB5
- STRING:   Q1GFB5
- GeneID:   4076869
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1918
- NMPDR:   fig|292414.1.peg.307
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   ICTVIAN
- PhylomeDB:   Q1GFB5
- ProtClustDB:   PRK00012
- BioCyc:   SSP292414:TM1040_1918-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52406; Mature: 52275

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLNKLGLAEARDLLRKGDTTSVELTEACLKAIDGADALNAFVHKTPDVALDRAKAADA
CCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCC
RIAEGDAPAMCGLPIGMKDLFCTKGVDSQAASNILKGFKPEYESTVSQKLQDAGAVMLGK
EECCCCCCCEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEC
LNMDEFAMGSSNETSAYGNAISPWRREGDETPLTPGGSSGGSAAAVAADLCLAATGTDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKNVRDAAIMLEAMCGHDAK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DSTSVDLAVPNFEAMLTGDIKGKKIGIPREYRMDGMPAEIAKLWDEGAAMLKAAGAEIVD
CCCEEEEECCCCEEEEECCCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRYGHRATLAQGDGITEMYEKTRAEGFGHE
EECCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHH
VQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKKDFEDVFAAGVDAILTPATPSAAFGLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHCCCCC
MNDEDPVKMYLNDVFTVTVNLAGLPGISVPTGVDTQGLPLGLQLIGRPWDEGDLLNTAYA
CCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
LEQAAGFVAKPAQWW
HHHHCCCCCCCCCCC
>Mature Secondary Structure 
SDLNKLGLAEARDLLRKGDTTSVELTEACLKAIDGADALNAFVHKTPDVALDRAKAADA
CCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCC
RIAEGDAPAMCGLPIGMKDLFCTKGVDSQAASNILKGFKPEYESTVSQKLQDAGAVMLGK
EECCCCCCCEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEC
LNMDEFAMGSSNETSAYGNAISPWRREGDETPLTPGGSSGGSAAAVAADLCLAATGTDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMTKNVRDAAIMLEAMCGHDAK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
DSTSVDLAVPNFEAMLTGDIKGKKIGIPREYRMDGMPAEIAKLWDEGAAMLKAAGAEIVD
CCCEEEEECCCCEEEEECCCCCCEECCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEE
ISLPHTKYALPAYYVIAPAEASSNLARYDGVRYGHRATLAQGDGITEMYEKTRAEGFGHE
EECCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHH
VQRRVMVGTYVLSAGFYDAYYNRARKVRTLIKKDFEDVFAAGVDAILTPATPSAAFGLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHCCCCC
MNDEDPVKMYLNDVFTVTVNLAGLPGISVPTGVDTQGLPLGLQLIGRPWDEGDLLNTAYA
CCCCCHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHH
LEQAAGFVAKPAQWW
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA