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The map label for this gene is amiD [H]

Identifier: 99081757

GI number: 99081757

Start: 2017873

End: 2018532

Strand: Reverse

Name: amiD [H]

Synonym: TM1040_1916

Alternate gene names: 99081757

Gene position: 2018532-2017873 (Counterclockwise)

Preceding gene: 99081758

Following gene: 99081756

Centisome position: 63.06

GC content: 66.52

Gene sequence:

>660_bases
ATGGTCTCTGCTGACCCCATCTGGCACCCGAGCCCCAACTTCGGGGCCCGGAGGGACGGGCTGCAGCCGCATCTCATTGT
TCTGCACTACACGGCAATGGAAAGCGCCGAAGCCGCGCTCCAGCGGCTCTGTGATCCCGCCTTCGAGGTCTCGGCGCATT
ATCTGATCGGCGGCGATGGCCGTCTTTGGCAGATGGTCGCAGAAGACCACCGCGCCTGGCACGCGGGCCAAGGAGAATGG
GCGGGACTTTCGGACATCAACTCGCGTTCGATCGGCATCGAGCTCGATAATCGCGGCGACCACCCGTTTTCAGAGCCTCA
GATGTGCGTGCTTGAGACGCTCATGGCGCAGATCATGACGCGCTGGACCATCCCGGCCACCGGTGTGATCGGTCACTCTG
ACATGGCGCCGGGGCGCAAATGGGATCCGGGGCCGCGCTTCGACTGGCAGCGGCTCGCCACCCTTGGTCTTGCGCGGCCC
AATGGCCAGCCGCTTGCCGCGCCCGCCGCCACGCCTGAGGCTTTGCGCAAGGCCGCGCAGGCCTGTGGCTTCACCGCCGA
GGTTGACGACGCCACGCTTCTTCAGGCTGTACGCCTGCGCTACCGCCCCTGGGCACGCGGCCCCCTCTGCGGCGAGGACA
TTGCGCCACTTCTCTCGTAG

Upstream 100 bases:

>100_bases
CCGCAAAGCGCTTGATCCCGATGGTGATGGCTACGCCTGTGGCTGGGATCCCGCGCCCTTCCGGGCTGCCGTTCGCGCGG
CGGGCAACTGATCTCTTACC

Downstream 100 bases:

>100_bases
CTCTTCTTCTAGCGCCAAATATCCTGGGGTGAATGCGCGCCCGCGCAGAGGGGCAAAGCCCCTTCACCCCATAGCATCTT
GACGCACCCTGCGCGCAGGC

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MVSADPIWHPSPNFGARRDGLQPHLIVLHYTAMESAEAALQRLCDPAFEVSAHYLIGGDGRLWQMVAEDHRAWHAGQGEW
AGLSDINSRSIGIELDNRGDHPFSEPQMCVLETLMAQIMTRWTIPATGVIGHSDMAPGRKWDPGPRFDWQRLATLGLARP
NGQPLAAPAATPEALRKAAQACGFTAEVDDATLLQAVRLRYRPWARGPLCGEDIAPLLS

Sequences:

>Translated_219_residues
MVSADPIWHPSPNFGARRDGLQPHLIVLHYTAMESAEAALQRLCDPAFEVSAHYLIGGDGRLWQMVAEDHRAWHAGQGEW
AGLSDINSRSIGIELDNRGDHPFSEPQMCVLETLMAQIMTRWTIPATGVIGHSDMAPGRKWDPGPRFDWQRLATLGLARP
NGQPLAAPAATPEALRKAAQACGFTAEVDDATLLQAVRLRYRPWARGPLCGEDIAPLLS
>Mature_219_residues
MVSADPIWHPSPNFGARRDGLQPHLIVLHYTAMESAEAALQRLCDPAFEVSAHYLIGGDGRLWQMVAEDHRAWHAGQGEW
AGLSDINSRSIGIELDNRGDHPFSEPQMCVLETLMAQIMTRWTIPATGVIGHSDMAPGRKWDPGPRFDWQRLATLGLARP
NGQPLAAPAATPEALRKAAQACGFTAEVDDATLLQAVRLRYRPWARGPLCGEDIAPLLS

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=156, Percent_Identity=41.025641025641, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786300, Length=163, Percent_Identity=34.9693251533742, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 24002; Mature: 24002

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSADPIWHPSPNFGARRDGLQPHLIVLHYTAMESAEAALQRLCDPAFEVSAHYLIGGDG
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHCCEEEEECCCC
RLWQMVAEDHRAWHAGQGEWAGLSDINSRSIGIELDNRGDHPFSEPQMCVLETLMAQIMT
HHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
RWTIPATGVIGHSDMAPGRKWDPGPRFDWQRLATLGLARPNGQPLAAPAATPEALRKAAQ
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ACGFTAEVDDATLLQAVRLRYRPWARGPLCGEDIAPLLS
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MVSADPIWHPSPNFGARRDGLQPHLIVLHYTAMESAEAALQRLCDPAFEVSAHYLIGGDG
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCHHHHCCEEEEECCCC
RLWQMVAEDHRAWHAGQGEWAGLSDINSRSIGIELDNRGDHPFSEPQMCVLETLMAQIMT
HHHHHHHHHHHHEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
RWTIPATGVIGHSDMAPGRKWDPGPRFDWQRLATLGLARPNGQPLAAPAATPEALRKAAQ
HCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
ACGFTAEVDDATLLQAVRLRYRPWARGPLCGEDIAPLLS
HCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]