Click here to switch to the map view.

The map label for this gene is guaD [H]

Identifier: 99081746

GI number: 99081746

Start: 2007845

End: 2009134

Strand: Direct

Name: guaD [H]

Synonym: TM1040_1905

Alternate gene names: 99081746

Gene position: 2007845-2009134 (Clockwise)

Preceding gene: 99081743

Following gene: 99081751

Centisome position: 62.73

GC content: 64.5

Gene sequence:

>1290_bases
ATGAAACAAGATCTCATTCTTCTGGGCCGAGTGCTGTCTTTTTGCGCCTCCCCTTTCGCGCCCGATGGGCCCGAGGCGGC
CGTCGAGGAACACGAAGCCATTGCCCTGCGCGCGGGCAAGATCGTGGCGCTTGGCAGCCGGGCAGCGCTCAGCAAGGTGC
TGCCAGAGGCAGAGATCGTCGATCACGGGCAGAAGCTGATCGTGCCGGGCTTTGTCGATGCCCATGTGCATTATCCGCAG
ACCGCGATCATCGCCAGCTGGGGCAAGCGGCTGATCGACTGGCTCAACACCTATACCTTTCCCGAGGAGATGCGGTTTGG
CAACCCGGCCTATGCCGCAGAGATCGCAGCGCGCTACCTGGATCTGACCACCGCCTGCGGCACCACGACGGTTGCGAGCT
ACTGCACCATCCATCCCGAGAGCGTTGATGCGTTGTTCGAGGCGGCGCAGGCCCGCGGGCAGCGCGTGGTGGCCGGCAAG
ACCTGCATGGACCGCAACGCGCCCGAAGGCCTGCGCGATACGCCGCAATCCGCCTATGACGACAGCGCCGCGCTGATCCA
GCGCTGGCATGGGAGGGAGCGGCTCATCTATGCGATTACGCCACGGTTTTCACCAACCTCCACCCCAGAACAACTCGAAG
CGCTCGGCGCACTCTGGACGGCGCATCCTACCTGCCTGATGCAGACCCATCTGAGCGAGCAACTGGACGAGATCGAATGG
GTCCGAACCCTCTTTCCCGAGGCGCGCGACTACCTTGACACCTACGAGCGCTACGGTCTTCTGCGCGAAGGCGCGCTCTT
TGGCCATGCCATCCATCTTGAACCCCGCGAACGCGCCCGCCTCCTGGAGGCCCGCGCCAGCCTGATCCACTGCCCCACCT
CCAATACCTTCATCGGTTCAGGGCTTTTTGACATGAACGGCCTCATGCGCGAGGGCCACCGGATCGGCCTTGCCACCGAC
ACCGGCGGCGGGTCGAGTTTTTCGATGCTGCGTACCATGGCCGCCGCCTATGAGGTCGCCCAGCTGCGGGGCACGCCTTT
GCACGCGGCACAGCTCCTGTGGCTTGCAACCCTCGGGTCGGCCACGGCACTTGGGCTTCAGGACAAGGTCGGTAATCTCG
CCGTGGGACGGGAGGCCGACCTTGTGGTGCTTGATCTTGCCTCGACCCCGGCCATTGCCCAGCGTGCGACACGCGCAGAG
ACCCTCTGGGAGGCCCTCTTTCCCACGCTGATGATGGGCGATGACCGCGCCATTGCCGAGGTCCGCATCAACGGGCAGAA
AGCGGTCTAG

Upstream 100 bases:

>100_bases
GATGCGGAAGGGCAAAAACAACGGCCCATCAGCAATTTACCCCTGCCGCCCCACGCCCTATCCTGTGCAGAGCTGATTTG
ACAACGAATGTGACATCGCA

Downstream 100 bases:

>100_bases
CAAAAAGGGGCGCAGCCGCGCGCCCCTCTTCTTCTAACCAAAATATCCCGGAGCGCGAGGCAGAGCCTCGCATCCCCGCT
CGCGCCCAACCGCGACGCTT

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKQDLILLGRVLSFCASPFAPDGPEAAVEEHEAIALRAGKIVALGSRAALSKVLPEAEIVDHGQKLIVPGFVDAHVHYPQ
TAIIASWGKRLIDWLNTYTFPEEMRFGNPAYAAEIAARYLDLTTACGTTTVASYCTIHPESVDALFEAAQARGQRVVAGK
TCMDRNAPEGLRDTPQSAYDDSAALIQRWHGRERLIYAITPRFSPTSTPEQLEALGALWTAHPTCLMQTHLSEQLDEIEW
VRTLFPEARDYLDTYERYGLLREGALFGHAIHLEPRERARLLEARASLIHCPTSNTFIGSGLFDMNGLMREGHRIGLATD
TGGGSSFSMLRTMAAAYEVAQLRGTPLHAAQLLWLATLGSATALGLQDKVGNLAVGREADLVVLDLASTPAIAQRATRAE
TLWEALFPTLMMGDDRAIAEVRINGQKAV

Sequences:

>Translated_429_residues
MKQDLILLGRVLSFCASPFAPDGPEAAVEEHEAIALRAGKIVALGSRAALSKVLPEAEIVDHGQKLIVPGFVDAHVHYPQ
TAIIASWGKRLIDWLNTYTFPEEMRFGNPAYAAEIAARYLDLTTACGTTTVASYCTIHPESVDALFEAAQARGQRVVAGK
TCMDRNAPEGLRDTPQSAYDDSAALIQRWHGRERLIYAITPRFSPTSTPEQLEALGALWTAHPTCLMQTHLSEQLDEIEW
VRTLFPEARDYLDTYERYGLLREGALFGHAIHLEPRERARLLEARASLIHCPTSNTFIGSGLFDMNGLMREGHRIGLATD
TGGGSSFSMLRTMAAAYEVAQLRGTPLHAAQLLWLATLGSATALGLQDKVGNLAVGREADLVVLDLASTPAIAQRATRAE
TLWEALFPTLMMGDDRAIAEVRINGQKAV
>Mature_429_residues
MKQDLILLGRVLSFCASPFAPDGPEAAVEEHEAIALRAGKIVALGSRAALSKVLPEAEIVDHGQKLIVPGFVDAHVHYPQ
TAIIASWGKRLIDWLNTYTFPEEMRFGNPAYAAEIAARYLDLTTACGTTTVASYCTIHPESVDALFEAAQARGQRVVAGK
TCMDRNAPEGLRDTPQSAYDDSAALIQRWHGRERLIYAITPRFSPTSTPEQLEALGALWTAHPTCLMQTHLSEQLDEIEW
VRTLFPEARDYLDTYERYGLLREGALFGHAIHLEPRERARLLEARASLIHCPTSNTFIGSGLFDMNGLMREGHRIGLATD
TGGGSSFSMLRTMAAAYEVAQLRGTPLHAAQLLWLATLGSATALGLQDKVGNLAVGREADLVVLDLASTPAIAQRATRAE
TLWEALFPTLMMGDDRAIAEVRINGQKAV

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=428, Percent_Identity=31.7757009345794, Blast_Score=201, Evalue=8e-52,
Organism=Escherichia coli, GI1789249, Length=420, Percent_Identity=42.3809523809524, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319963, Length=335, Percent_Identity=35.2238805970149, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24643849, Length=424, Percent_Identity=34.4339622641509, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 46829; Mature: 46829

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQDLILLGRVLSFCASPFAPDGPEAAVEEHEAIALRAGKIVALGSRAALSKVLPEAEIV
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEECCCEEEECCHHHHHHHCCCHHHH
DHGQKLIVPGFVDAHVHYPQTAIIASWGKRLIDWLNTYTFPEEMRFGNPAYAAEIAARYL
HCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
DLTTACGTTTVASYCTIHPESVDALFEAAQARGQRVVAGKTCMDRNAPEGLRDTPQSAYD
HHHHHCCHHHHHHHEEECHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCHHCC
DSAALIQRWHGRERLIYAITPRFSPTSTPEQLEALGALWTAHPTCLMQTHLSEQLDEIEW
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VRTLFPEARDYLDTYERYGLLREGALFGHAIHLEPRERARLLEARASLIHCPTSNTFIGS
HHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHHHEEECCCCCCEECC
GLFDMNGLMREGHRIGLATDTGGGSSFSMLRTMAAAYEVAQLRGTPLHAAQLLWLATLGS
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ATALGLQDKVGNLAVGREADLVVLDLASTPAIAQRATRAETLWEALFPTLMMGDDRAIAE
HHCCCCHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VRINGQKAV
EEECCCCCC
>Mature Secondary Structure
MKQDLILLGRVLSFCASPFAPDGPEAAVEEHEAIALRAGKIVALGSRAALSKVLPEAEIV
CCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEEECCCEEEECCHHHHHHHCCCHHHH
DHGQKLIVPGFVDAHVHYPQTAIIASWGKRLIDWLNTYTFPEEMRFGNPAYAAEIAARYL
HCCCEEEECCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
DLTTACGTTTVASYCTIHPESVDALFEAAQARGQRVVAGKTCMDRNAPEGLRDTPQSAYD
HHHHHCCHHHHHHHEEECHHHHHHHHHHHHHCCCEEEECCHHHCCCCCCCCCCCCCHHCC
DSAALIQRWHGRERLIYAITPRFSPTSTPEQLEALGALWTAHPTCLMQTHLSEQLDEIEW
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VRTLFPEARDYLDTYERYGLLREGALFGHAIHLEPRERARLLEARASLIHCPTSNTFIGS
HHHHHHHHHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHHHEEECCCCCCEECC
GLFDMNGLMREGHRIGLATDTGGGSSFSMLRTMAAAYEVAQLRGTPLHAAQLLWLATLGS
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
ATALGLQDKVGNLAVGREADLVVLDLASTPAIAQRATRAETLWEALFPTLMMGDDRAIAE
HHCCCCHHCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
VRINGQKAV
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]