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The map label for this gene is dctD [H]

Identifier: 99081655

GI number: 99081655

Start: 1906276

End: 1907505

Strand: Reverse

Name: dctD [H]

Synonym: TM1040_1814

Alternate gene names: 99081655

Gene position: 1907505-1906276 (Counterclockwise)

Preceding gene: 99081656

Following gene: 99081654

Centisome position: 59.59

GC content: 66.59

Gene sequence:

>1230_bases
ATGACGCGCAAGGTGCTGTTGGTTGATGATGATGCGGCCGTTCGCGAGGCGCTGGGCCAGACGCTCGAGCTTGCCGATCT
CAACGCGGTGACGGCGGGGTCGTTTGTGGCCGCAAAGGACCACATCACCCCGGATTTCGAGGGGGTGATCCTGTCGGATA
TGCGGATGCCGGGGCGTGATGGGTTCCATCTGTTGTCCTACGCACAAGAGGTGGATGCGGAGCTGCCGGTGGTGCTGCTG
ACGGGCGAGGGCGACATTCCCATGGCGGTGCGGGCGATGTCCGAGGGGGCTTTTGATTTTCTCGAAAAACCCTGCGAGGC
GGCCACGCTGATGACGGTGCTGGAACGTGCGCTGAAGACGCGCGCGCTTGTGCTGGAAAACCGCCGCCTGCAACGCCAGC
TGGAGCGGGGGGACCCGGCGGCGCGGATGATCTTTGGCGTCTCGCCCCAGATCCAGGAGCTGCGCGCGCGGGTGCGGGCT
GCGGCGGCGACCGACGCCGAAGTGCTGGTGAGTGGCGCGCCCGGCAGTGGCATTCCCAAGATCGCCGAGGTCGTGCATCT
GATGTCGCGGCGCGGGCAGGGGGCGTTTGTCAAACGTCCGGCGGCGGGGCTGACCCCTCAGACCTTCGAGGAGCTTTGCC
AGACCGCCGAGGGCGGATCCCTGTTCCTCGATGAGCTGTCGGCCATGCCGCAAGAGACGCAATATGCGGTGCTTGCGCGG
CTCGAGCAGGGGGGCGGTGCGCGCATCATCGGCGGTTCGACCGTGGATCTGTCGGAACGTGTGGCGGGCGGCAGTTTCAA
CGCCGAGCTTTACTATCGCCTCGACGTGATGCGGGTGCGGGTGCCCTCGCTTGCCGAGCGGCGCGAGGATATTCCGGTGC
TCTTTCGCCATTATGTGAGCCAGGCCGCCGAGCAGGCGGGGATCGACGCCCCGGAGATCAGCCCCGATACGCTGGCAAGC
CTGATGGCGCAGGACTGGCCCGGAAATGCGCGCTCCTTGATGTCGGCGGCGATGCGCTTTGTGCTTGGCATGCCCGAAGA
AGTCGCGCAGGCGACCGATCTGGGGCTCGCGGAACAGATGGCGCAGGTCGAGCGCTCGCTGCTGATTGCGGCCCTCGGGC
GGCAGAACGGCAAGGCGGCAGCCGCCGCCGAAGCGCTGAAACTGCCGCGCAAGACCTTCTATGACAAGCTGGCCCGCTAT
GGGATTCGCCCCGAGGACTATCGGCGCTAA

Upstream 100 bases:

>100_bases
GACTGGTGCAAAGCTTTGGCGGCAATATCCGGGGCACCAACACAGAGCACGGCGCCCTGTTCACGGTCGAACTGGACTAC
TGGAAAGAGGAAACCGCCGC

Downstream 100 bases:

>100_bases
CGGCTGTGCGGATTTTCGCACAAGCCGCAGGGTGCGTGTGTGAAATCCCGCACAGGGCCAGGGGAGAGCCGCCCCCGACA
GCACAAGTCCTCTCTCTAGG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRKVLLVDDDAAVREALGQTLELADLNAVTAGSFVAAKDHITPDFEGVILSDMRMPGRDGFHLLSYAQEVDAELPVVLL
TGEGDIPMAVRAMSEGAFDFLEKPCEAATLMTVLERALKTRALVLENRRLQRQLERGDPAARMIFGVSPQIQELRARVRA
AAATDAEVLVSGAPGSGIPKIAEVVHLMSRRGQGAFVKRPAAGLTPQTFEELCQTAEGGSLFLDELSAMPQETQYAVLAR
LEQGGGARIIGGSTVDLSERVAGGSFNAELYYRLDVMRVRVPSLAERREDIPVLFRHYVSQAAEQAGIDAPEISPDTLAS
LMAQDWPGNARSLMSAAMRFVLGMPEEVAQATDLGLAEQMAQVERSLLIAALGRQNGKAAAAAEALKLPRKTFYDKLARY
GIRPEDYRR

Sequences:

>Translated_409_residues
MTRKVLLVDDDAAVREALGQTLELADLNAVTAGSFVAAKDHITPDFEGVILSDMRMPGRDGFHLLSYAQEVDAELPVVLL
TGEGDIPMAVRAMSEGAFDFLEKPCEAATLMTVLERALKTRALVLENRRLQRQLERGDPAARMIFGVSPQIQELRARVRA
AAATDAEVLVSGAPGSGIPKIAEVVHLMSRRGQGAFVKRPAAGLTPQTFEELCQTAEGGSLFLDELSAMPQETQYAVLAR
LEQGGGARIIGGSTVDLSERVAGGSFNAELYYRLDVMRVRVPSLAERREDIPVLFRHYVSQAAEQAGIDAPEISPDTLAS
LMAQDWPGNARSLMSAAMRFVLGMPEEVAQATDLGLAEQMAQVERSLLIAALGRQNGKAAAAAEALKLPRKTFYDKLARY
GIRPEDYRR
>Mature_408_residues
TRKVLLVDDDAAVREALGQTLELADLNAVTAGSFVAAKDHITPDFEGVILSDMRMPGRDGFHLLSYAQEVDAELPVVLLT
GEGDIPMAVRAMSEGAFDFLEKPCEAATLMTVLERALKTRALVLENRRLQRQLERGDPAARMIFGVSPQIQELRARVRAA
AATDAEVLVSGAPGSGIPKIAEVVHLMSRRGQGAFVKRPAAGLTPQTFEELCQTAEGGSLFLDELSAMPQETQYAVLARL
EQGGGARIIGGSTVDLSERVAGGSFNAELYYRLDVMRVRVPSLAERREDIPVLFRHYVSQAAEQAGIDAPEISPDTLASL
MAQDWPGNARSLMSAAMRFVLGMPEEVAQATDLGLAEQMAQVERSLLIAALGRQNGKAAAAAEALKLPRKTFYDKLARYG
IRPEDYRR

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=471, Percent_Identity=30.7855626326964, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=29.3216630196937, Blast_Score=181, Evalue=9e-47,
Organism=Escherichia coli, GI1788905, Length=448, Percent_Identity=30.1339285714286, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=28.6036036036036, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=30.421686746988, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87082117, Length=351, Percent_Identity=29.0598290598291, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789233, Length=323, Percent_Identity=26.625386996904, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=25.2307692307692, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786524, Length=315, Percent_Identity=27.9365079365079, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=27.7056277056277, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081858, Length=307, Percent_Identity=25.7328990228013, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=27.0440251572327, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 44333; Mature: 44202

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKVLLVDDDAAVREALGQTLELADLNAVTAGSFVAAKDHITPDFEGVILSDMRMPGRD
CCCEEEEECCCHHHHHHHCCCEEHHCCCHHCCCCEEEECCCCCCCCCCEEEECCCCCCCC
GFHLLSYAQEVDAELPVVLLTGEGDIPMAVRAMSEGAFDFLEKPCEAATLMTVLERALKT
CHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHH
RALVLENRRLQRQLERGDPAARMIFGVSPQIQELRARVRAAAATDAEVLVSGAPGSGIPK
HHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
IAEVVHLMSRRGQGAFVKRPAAGLTPQTFEELCQTAEGGSLFLDELSAMPQETQYAVLAR
HHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHH
LEQGGGARIIGGSTVDLSERVAGGSFNAELYYRLDVMRVRVPSLAERREDIPVLFRHYVS
HHCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEEEHHHHCCCHHHHHHCCCHHHHHHHHH
QAAEQAGIDAPEISPDTLASLMAQDWPGNARSLMSAAMRFVLGMPEEVAQATDLGLAEQM
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
AQVERSLLIAALGRQNGKAAAAAEALKLPRKTFYDKLARYGIRPEDYRR
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
TRKVLLVDDDAAVREALGQTLELADLNAVTAGSFVAAKDHITPDFEGVILSDMRMPGRD
CCEEEEECCCHHHHHHHCCCEEHHCCCHHCCCCEEEECCCCCCCCCCEEEECCCCCCCC
GFHLLSYAQEVDAELPVVLLTGEGDIPMAVRAMSEGAFDFLEKPCEAATLMTVLERALKT
CHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHH
RALVLENRRLQRQLERGDPAARMIFGVSPQIQELRARVRAAAATDAEVLVSGAPGSGIPK
HHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHH
IAEVVHLMSRRGQGAFVKRPAAGLTPQTFEELCQTAEGGSLFLDELSAMPQETQYAVLAR
HHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHH
LEQGGGARIIGGSTVDLSERVAGGSFNAELYYRLDVMRVRVPSLAERREDIPVLFRHYVS
HHCCCCEEEECCCCCCHHHHHCCCCCCCEEEEEEEHHHHCCCHHHHHHCCCHHHHHHHHH
QAAEQAGIDAPEISPDTLASLMAQDWPGNARSLMSAAMRFVLGMPEEVAQATDLGLAEQM
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
AQVERSLLIAALGRQNGKAAAAAEALKLPRKTFYDKLARYGIRPEDYRR
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]