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The map label for this gene is phbC [H]

Identifier: 99081556

GI number: 99081556

Start: 1812653

End: 1814482

Strand: Reverse

Name: phbC [H]

Synonym: TM1040_1715

Alternate gene names: 99081556

Gene position: 1814482-1812653 (Counterclockwise)

Preceding gene: 99081559

Following gene: 99081546

Centisome position: 56.69

GC content: 56.83

Gene sequence:

>1830_bases
ATGACAACAACGGACAAGTCTATTGAAGCTTCATCTGCGGAACAGATTGACCAGCTTAAAGAAAATTTGGTGCGCGTAGA
GCAACTCTCCAAGCGTTTGATCGAAGTTTTGGCTCAGAAGAAGGGTCACAACCCCGGATTGGACGCTCCGAATCAGGAAC
TTTTCGCGCGTGCAGCATCGTCCTATTGGGCTGCGATGGCACAGAACCCCACCCGCCTGCTTGAACAACAAGTTCAATAT
TGGGGCAAGTCGGTGCAAAATTTTGCAGAAGCGCAGCAGGCCATGGCACAGACGCTGGCCGCCGCGCAGAATGAGGAGGG
CGAGACTACAGAGCGCCCCGCGGTAAACGATCGTCGGTTCGCCAATCCGATGTGGGAGACCAATCCCTATTTCCGGTTCC
TGCGCGATCAGTACCTGACCAATGCGGAGGCCATCCGTACCGCTGTGGACGAGGTCGAAGATCTCGATCGGGTGGATCGG
CAGCGGCTCGGTTATTTTGCCGATCAGATCATCAACATGATGGCTCCGACCAATTTCCTCGCGACAAACCCGGACGCTCT
GGAAAAGGCGCTGGAAACTGACGGGCAATCCCTGATCGACGGGTTGGAGAATCTCGTGGCCGATCTGGAGGCCAATGATG
GCGAGTTGATCGTTCGCCTCGCCGATGAGAACGCCTTTGAGATCGGTCACAACATCGCCACCACCCCCGGCGAGGTGGTC
TATCGCAACCGGATGATGGAGCTTATCCAGTATCATCCCACCACCGAGACGGTGCACGAAACGCCCATCGTGCTGTTCCC
GCCCTGGATCAACAAGTTCTACATCCTCGATCTCAAGGAAAAGAACAGCCTGATCAAATGGATCACAGATCAGGGTTACA
GTCTGTTTGTGGTCTCATGGATCAATCCCAATTCGGACTATGCTGATGTCGGCATGGAGGATTACATCGAGGAGGGGTTC
CTCAAGGCCATCGAGGTCGCCAAGGAGATCTCCGGCACCAAGCAAGTGAATGCAGTGGGCTACTGCATTGCGGGCACCAC
GCTCAGCCTGACGCTGTCGCTCCTGAAACAGCGCGGCGACAAATCGGTGAAGTCCGCGACATTCTTTACCGCGCTCACCG
ATTTCTCCGATCAGGGGGATTTTACGCCCTTCCTGCAAAACGATTTTGTCGATGCGATCGAAGCGGAGATCGGCGAAGAC
GGGATCATGCCGCACTACATCATGGCGCGGACTTTCTCGTTCCTGAGGGCCAATGATCTGGTCTATGGCCCGGCCATCCG
CAGCTATATGATGGGCGAGACCCCACCGGCCTTTGATCTTCTTTATTGGAATGGCGACGGCGCCAATCTGCCGGGAAAAA
TGGCGGTGCAGTATTTGCGGGGGCTCTGCCAGGACAACAAGTTTGTGACCGACGGGTTCGAGCTCTTTGGGCATACGCTG
CATGTGCGCGATGTGGACGTGCCGCTGATGGCGATCACCTGCGAGACCGACCACATTGCGGCGTGGAAACAATGCTATCG
CGGCGTGCAACAGATGGGCTCGCGCTCCAAGAGCTTTGTGGTGAGCCAGTCCGGTCACATCGCGGGTATCGTCAATCCCG
TGAGCCGCAACAAATACGGTCATTATGTCAACGGCGATCTCAGTCTCGATGCCGACGACTGGATGGAGGGCGCAGAGTTC
ACCGAAGGATCCTGGTGGCCGCGCTGGGAGGCGTGGCTCAAGAAACGTTCGGGCAAGCAGGTGCCCGCGCGGGTGCCAGG
GGCGCATAAATCCTATCCGGTGCTTGAACAGGCGCCGGGCAACTATGTGCGTGCCAAGGCGCGTGGATAA

Upstream 100 bases:

>100_bases
TGCCGACGCACGCCGGGAGATTGGGAGCTGAGGCCGCTTTGCCGTAGAATGGCGAAGCAAGTATTCTGCACAGCAGAAAG
ATTATGAGGGTTTCCTTAAG

Downstream 100 bases:

>100_bases
GAAAACGCCCCGCTGATCTGATCAGCGGGGCGTTTTTTCTTTGCGGGTGTCGCGCCCTTAGCGCATCAGCTGCGGGCGCA
CCCGGATCTTGCGGCGCAGC

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTTTDKSIEASSAEQIDQLKENLVRVEQLSKRLIEVLAQKKGHNPGLDAPNQELFARAASSYWAAMAQNPTRLLEQQVQY
WGKSVQNFAEAQQAMAQTLAAAQNEEGETTERPAVNDRRFANPMWETNPYFRFLRDQYLTNAEAIRTAVDEVEDLDRVDR
QRLGYFADQIINMMAPTNFLATNPDALEKALETDGQSLIDGLENLVADLEANDGELIVRLADENAFEIGHNIATTPGEVV
YRNRMMELIQYHPTTETVHETPIVLFPPWINKFYILDLKEKNSLIKWITDQGYSLFVVSWINPNSDYADVGMEDYIEEGF
LKAIEVAKEISGTKQVNAVGYCIAGTTLSLTLSLLKQRGDKSVKSATFFTALTDFSDQGDFTPFLQNDFVDAIEAEIGED
GIMPHYIMARTFSFLRANDLVYGPAIRSYMMGETPPAFDLLYWNGDGANLPGKMAVQYLRGLCQDNKFVTDGFELFGHTL
HVRDVDVPLMAITCETDHIAAWKQCYRGVQQMGSRSKSFVVSQSGHIAGIVNPVSRNKYGHYVNGDLSLDADDWMEGAEF
TEGSWWPRWEAWLKKRSGKQVPARVPGAHKSYPVLEQAPGNYVRAKARG

Sequences:

>Translated_609_residues
MTTTDKSIEASSAEQIDQLKENLVRVEQLSKRLIEVLAQKKGHNPGLDAPNQELFARAASSYWAAMAQNPTRLLEQQVQY
WGKSVQNFAEAQQAMAQTLAAAQNEEGETTERPAVNDRRFANPMWETNPYFRFLRDQYLTNAEAIRTAVDEVEDLDRVDR
QRLGYFADQIINMMAPTNFLATNPDALEKALETDGQSLIDGLENLVADLEANDGELIVRLADENAFEIGHNIATTPGEVV
YRNRMMELIQYHPTTETVHETPIVLFPPWINKFYILDLKEKNSLIKWITDQGYSLFVVSWINPNSDYADVGMEDYIEEGF
LKAIEVAKEISGTKQVNAVGYCIAGTTLSLTLSLLKQRGDKSVKSATFFTALTDFSDQGDFTPFLQNDFVDAIEAEIGED
GIMPHYIMARTFSFLRANDLVYGPAIRSYMMGETPPAFDLLYWNGDGANLPGKMAVQYLRGLCQDNKFVTDGFELFGHTL
HVRDVDVPLMAITCETDHIAAWKQCYRGVQQMGSRSKSFVVSQSGHIAGIVNPVSRNKYGHYVNGDLSLDADDWMEGAEF
TEGSWWPRWEAWLKKRSGKQVPARVPGAHKSYPVLEQAPGNYVRAKARG
>Mature_608_residues
TTTDKSIEASSAEQIDQLKENLVRVEQLSKRLIEVLAQKKGHNPGLDAPNQELFARAASSYWAAMAQNPTRLLEQQVQYW
GKSVQNFAEAQQAMAQTLAAAQNEEGETTERPAVNDRRFANPMWETNPYFRFLRDQYLTNAEAIRTAVDEVEDLDRVDRQ
RLGYFADQIINMMAPTNFLATNPDALEKALETDGQSLIDGLENLVADLEANDGELIVRLADENAFEIGHNIATTPGEVVY
RNRMMELIQYHPTTETVHETPIVLFPPWINKFYILDLKEKNSLIKWITDQGYSLFVVSWINPNSDYADVGMEDYIEEGFL
KAIEVAKEISGTKQVNAVGYCIAGTTLSLTLSLLKQRGDKSVKSATFFTALTDFSDQGDFTPFLQNDFVDAIEAEIGEDG
IMPHYIMARTFSFLRANDLVYGPAIRSYMMGETPPAFDLLYWNGDGANLPGKMAVQYLRGLCQDNKFVTDGFELFGHTLH
VRDVDVPLMAITCETDHIAAWKQCYRGVQQMGSRSKSFVVSQSGHIAGIVNPVSRNKYGHYVNGDLSLDADDWMEGAEFT
EGSWWPRWEAWLKKRSGKQVPARVPGAHKSYPVLEQAPGNYVRAKARG

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 68471; Mature: 68339

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTDKSIEASSAEQIDQLKENLVRVEQLSKRLIEVLAQKKGHNPGLDAPNQELFARAAS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SYWAAMAQNPTRLLEQQVQYWGKSVQNFAEAQQAMAQTLAAAQNEEGETTERPAVNDRRF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ANPMWETNPYFRFLRDQYLTNAEAIRTAVDEVEDLDRVDRQRLGYFADQIINMMAPTNFL
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATNPDALEKALETDGQSLIDGLENLVADLEANDGELIVRLADENAFEIGHNIATTPGEVV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHH
YRNRMMELIQYHPTTETVHETPIVLFPPWINKFYILDLKEKNSLIKWITDQGYSLFVVSW
HHHHHHHHHHCCCCCCCHHCCCEEEECCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEE
INPNSDYADVGMEDYIEEGFLKAIEVAKEISGTKQVNAVGYCIAGTTLSLTLSLLKQRGD
ECCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECHHHHHHHHHHHHCCC
KSVKSATFFTALTDFSDQGDFTPFLQNDFVDAIEAEIGEDGIMPHYIMARTFSFLRANDL
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
VYGPAIRSYMMGETPPAFDLLYWNGDGANLPGKMAVQYLRGLCQDNKFVTDGFELFGHTL
EECHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECHHHHHCCEE
HVRDVDVPLMAITCETDHIAAWKQCYRGVQQMGSRSKSFVVSQSGHIAGIVNPVSRNKYG
EEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHCCCCC
HYVNGDLSLDADDWMEGAEFTEGSWWPRWEAWLKKRSGKQVPARVPGAHKSYPVLEQAPG
CEECCCEEECHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCC
NYVRAKARG
CEEEECCCC
>Mature Secondary Structure 
TTTDKSIEASSAEQIDQLKENLVRVEQLSKRLIEVLAQKKGHNPGLDAPNQELFARAAS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
SYWAAMAQNPTRLLEQQVQYWGKSVQNFAEAQQAMAQTLAAAQNEEGETTERPAVNDRRF
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
ANPMWETNPYFRFLRDQYLTNAEAIRTAVDEVEDLDRVDRQRLGYFADQIINMMAPTNFL
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ATNPDALEKALETDGQSLIDGLENLVADLEANDGELIVRLADENAFEIGHNIATTPGEVV
CCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHCCCCCCCCHHHH
YRNRMMELIQYHPTTETVHETPIVLFPPWINKFYILDLKEKNSLIKWITDQGYSLFVVSW
HHHHHHHHHHCCCCCCCHHCCCEEEECCCCCCEEEEEECCCCCHHHHHHCCCCEEEEEEE
INPNSDYADVGMEDYIEEGFLKAIEVAKEISGTKQVNAVGYCIAGTTLSLTLSLLKQRGD
ECCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECHHHHHHHHHHHHCCC
KSVKSATFFTALTDFSDQGDFTPFLQNDFVDAIEAEIGEDGIMPHYIMARTFSFLRANDL
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCC
VYGPAIRSYMMGETPPAFDLLYWNGDGANLPGKMAVQYLRGLCQDNKFVTDGFELFGHTL
EECHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECHHHHHCCEE
HVRDVDVPLMAITCETDHIAAWKQCYRGVQQMGSRSKSFVVSQSGHIAGIVNPVSRNKYG
EEEECCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHCCCCC
HYVNGDLSLDADDWMEGAEFTEGSWWPRWEAWLKKRSGKQVPARVPGAHKSYPVLEQAPG
CEECCCEEECHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCCCC
NYVRAKARG
CEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]