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The map label for this gene is ybfF [H]

Identifier: 99081427

GI number: 99081427

Start: 1694338

End: 1695096

Strand: Direct

Name: ybfF [H]

Synonym: TM1040_1586

Alternate gene names: 99081427

Gene position: 1694338-1695096 (Clockwise)

Preceding gene: 99081425

Following gene: 99081436

Centisome position: 52.93

GC content: 62.71

Gene sequence:

>759_bases
ATGCTGAACACGATCATACATGGCACCCCGACCGACCAACCGCCCCTCTTGATTGCCCATGGCCTCTATGGCTCCGGGCG
CAACTGGGGGGTGATTGCCAAACGACTCGCGGACGAGCGGCAAGTGATCGCGGTGGACATGCGCAACCACGGGCTGAGCC
CCAAGACCCAGAGCCACAGCTATGTCGATCTGGCCGAGGATCTGGCCGAAGTGATTGCGGCCCACGGCGGGCGCATGGAT
GTGGTCGGGCACTCGATGGGAGGCAAGGCCGCGATGATGCTGGCGCTGCGCCATCCCGAGGCGGTCAACCGCCTGGTGGT
CGCCGATATCGCACCGGTCAGCTATCAGCACACCCAGGCGCATTTCATCACTGCCATGCGCAGTGTCGACCTTGCGCGCG
TGACGCGGCGCTCGGACGCCCAAGAGCAGCTGGCGGCGGCTGGTGTGGATGCGATGTTGCAAAGCTTTTTCACCCAATCT
CTGGATGTGGAGAACAAGAGCTGGCGCCTCAACCTCGATACCTTGGAGCGGGAGATGCCGCATATTCTGTCGTTTCCGGA
GGTGGAGACCCATTGGGATGGGACGGCGCTGTTTCTGACCGGGGCGCATTCGGATTATGTGCTGCCAGAGCACCGCCCGC
AGATCAAACGCCTGTTTCCAGCGGCGCGCTTTGCCAAGATCCCCGGTGCAGGCCATTGGCTCCACGCGGAAAAACCACGC
GAGTTCGAGGCCACCGTACGCGGTTTCTTGAACGCGTGA

Upstream 100 bases:

>100_bases
TGACGCGATGCGCGCGACCTTAGCCGCGGCGGCGCGGGATCTCAATGCTGCTGCGCGGTTTGGGGCTGACACTGCCTGCA
GAGAGGGCTATCACCCCGTT

Downstream 100 bases:

>100_bases
ACGCAGCGCAGAAGCCCTGGGGGCGCTGCCCCCGGCCCTTGTCGGGCCTCCCCCGGGATATTTCTCGTTAGAAGAAGGAC
TTAGTAGGTCAAAAGCAGGC

Product: alpha/beta hydrolase fold

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLNTIIHGTPTDQPPLLIAHGLYGSGRNWGVIAKRLADERQVIAVDMRNHGLSPKTQSHSYVDLAEDLAEVIAAHGGRMD
VVGHSMGGKAAMMLALRHPEAVNRLVVADIAPVSYQHTQAHFITAMRSVDLARVTRRSDAQEQLAAAGVDAMLQSFFTQS
LDVENKSWRLNLDTLEREMPHILSFPEVETHWDGTALFLTGAHSDYVLPEHRPQIKRLFPAARFAKIPGAGHWLHAEKPR
EFEATVRGFLNA

Sequences:

>Translated_252_residues
MLNTIIHGTPTDQPPLLIAHGLYGSGRNWGVIAKRLADERQVIAVDMRNHGLSPKTQSHSYVDLAEDLAEVIAAHGGRMD
VVGHSMGGKAAMMLALRHPEAVNRLVVADIAPVSYQHTQAHFITAMRSVDLARVTRRSDAQEQLAAAGVDAMLQSFFTQS
LDVENKSWRLNLDTLEREMPHILSFPEVETHWDGTALFLTGAHSDYVLPEHRPQIKRLFPAARFAKIPGAGHWLHAEKPR
EFEATVRGFLNA
>Mature_252_residues
MLNTIIHGTPTDQPPLLIAHGLYGSGRNWGVIAKRLADERQVIAVDMRNHGLSPKTQSHSYVDLAEDLAEVIAAHGGRMD
VVGHSMGGKAAMMLALRHPEAVNRLVVADIAPVSYQHTQAHFITAMRSVDLARVTRRSDAQEQLAAAGVDAMLQSFFTQS
LDVENKSWRLNLDTLEREMPHILSFPEVETHWDGTALFLTGAHSDYVLPEHRPQIKRLFPAARFAKIPGAGHWLHAEKPR
EFEATVRGFLNA

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=248, Percent_Identity=42.741935483871, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI23200008, Length=248, Percent_Identity=42.741935483871, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI223941839, Length=240, Percent_Identity=36.6666666666667, Blast_Score=143, Evalue=1e-34,
Organism=Escherichia coli, GI1786902, Length=250, Percent_Identity=37.2, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17508535, Length=253, Percent_Identity=38.7351778656126, Blast_Score=170, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17507063, Length=249, Percent_Identity=28.5140562248996, Blast_Score=114, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6321468, Length=273, Percent_Identity=32.6007326007326, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640348, Length=262, Percent_Identity=34.3511450381679, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646055, Length=274, Percent_Identity=27.3722627737226, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 27926; Mature: 27926

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNTIIHGTPTDQPPLLIAHGLYGSGRNWGVIAKRLADERQVIAVDMRNHGLSPKTQSHS
CCCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
YVDLAEDLAEVIAAHGGRMDVVGHSMGGKAAMMLALRHPEAVNRLVVADIAPVSYQHTQA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCHHHHCEEEEEECCCCCCCHHHH
HFITAMRSVDLARVTRRSDAQEQLAAAGVDAMLQSFFTQSLDVENKSWRLNLDTLEREMP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
HILSFPEVETHWDGTALFLTGAHSDYVLPEHRPQIKRLFPAARFAKIPGAGHWLHAEKPR
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHCCCCCCCEECCCCCC
EFEATVRGFLNA
HHHHHHHHHHCC
>Mature Secondary Structure
MLNTIIHGTPTDQPPLLIAHGLYGSGRNWGVIAKRLADERQVIAVDMRNHGLSPKTQSHS
CCCCEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
YVDLAEDLAEVIAAHGGRMDVVGHSMGGKAAMMLALRHPEAVNRLVVADIAPVSYQHTQA
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCHHHHCEEEEEECCCCCCCHHHH
HFITAMRSVDLARVTRRSDAQEQLAAAGVDAMLQSFFTQSLDVENKSWRLNLDTLEREMP
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
HILSFPEVETHWDGTALFLTGAHSDYVLPEHRPQIKRLFPAARFAKIPGAGHWLHAEKPR
HHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHCCCCCCCEECCCCCC
EFEATVRGFLNA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]