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The map label for this gene is alkK [H]

Identifier: 99081406

GI number: 99081406

Start: 1671523

End: 1673154

Strand: Direct

Name: alkK [H]

Synonym: TM1040_1565

Alternate gene names: 99081406

Gene position: 1671523-1673154 (Clockwise)

Preceding gene: 99081403

Following gene: 99081408

Centisome position: 52.22

GC content: 59.87

Gene sequence:

>1632_bases
ATGGGGTGGTTGGCCGATGAAACCGGGTTGGAGCGCAGGGCTGCAAATTATGTCGCGCTGACGCCGCTGTCCCATTTGCA
GCGGGCGGCGCATGTCTTTGCCGACGAACCCGCCTTGGTCTACGGTCAGACTCGGCGCAGCTATGCCCAGTATCATGATC
GCTGCACGCGGTTGGCGTCGGCATTGGCCGGGATGGGGGTTGCTCCGGGCGATGTGGTGGCAACATTGCTGCCCAACATC
CCCGCACAGGCGGAGGCACATTTTGGCGTGCCAGCCTGCGGGGCGGTGCTCAATACGATCAACATTCGGCTGGATGTGGA
TACGGTGGCCTATATCTTTGATCACGGTCAGGCCAAAGTCGTGCTTGTGGACAGTGAATTCTTGCCTCTTGCCGAAGCCG
CCAAAGAAAGACTGCAGGGCGCAGGCCCTACCCTCATTGAAGTGCCGGACACAGAGGCTGGCCATGCGGCCAGCGGTCGC
TACACCACCTATGAAGACCTGATCGCGCATGGTGCAACTGATTTTGACTGGATCATGCCCGAGGATGAATGGGAGAGCCT
CGCGCTCAATTACACCTCTGGCACCACCGGGCGCCCGAAAGGCGTGGTCTACCATCACCGCGGCGCCTACCTGATGACCA
TGGGCACGGTGGTGTCGTGGCGGTTGCAACTGCGCCCGGTCTACCTGACCATCGTGCCGCTGTTTCATTGCAACGGCTGG
AACCACACTTGGATGATGCCGGTCCTGGGCGGCACCGTGGTTTGCTGTCGCAACACCACCGCCGAAGCCATCTATAACGC
CATCGCGGATGAGGGTGTCACCCATTTTGGCGGCGCGCCCATCGTGCTCAACATGATCGTGAACGCCAAGGACACCGAGC
GCCGCGCCTTTGACCACACGGTCGAAGTCTTTACCGCCGGTGCGCCCCCTGCCCCGGCCACGCTCGAGAAAATCGAACGC
CTGGGCTTTCATGTCACGCAAGTCTACGGGCTTACAGAGACCTATGGTCATGTGACGGAATGCCTCTGGAAAGGCGACAG
CTGGGACGAGCTGGATCAACAAGGGCGCGCCGCAATCAAGGCACGTCAGGGCGTGGCCTTTCCAATGATGGACCACATCA
CGGTCATGGACGCCGACATGCAACAGGTCCCCATGAATGGCAGCGATCAGGGGGAAATCGTGATGCGGGGCAACTCCGTC
ATGAAAGGTTACCTAAAGAACCCGGAAGCCACTGAAGAGGCGTTTGCGGGCGGCTATTTCCACTCTGGCGACATCGCGAT
TCAGCACCCGGATGGCTACATCCAGATCGCGGACCGCGCCAAGGACATCATTATCTCGGGCGGGGAGAACATCTCTTCCG
TTGAAGTCGAGGGGGTCCTGATGGGCCATCCGGATGTGAACCTTGCGGCGGTCGTGGCAAAACCGGACGAGAAATGGGGC
GAAGTGCCCTGCGCCTTTGTGGAACTCAAAGAAGGCGCAGATGTCGATGAGGCATCCCTAATTGCCTTTGCCCGCCAAAC
GCTTGCGGGCTTTAAGACACCCAAGGCAGTGGTCTTCTGCGAGCTCCCCAAGACCTCGACCGGAAAGATCCAGAAGTTTG
AACTGAGGAAGAGCTTCGCAGGGGGGAGTTGA

Upstream 100 bases:

>100_bases
AGGAATTGCAGCCAGCACCAAGCAATCTCTTTCAATTTCGAACGCCTCAGCGCAATAATCGCTCCCAGAGATAAAAACAC
TGAGCTTGGGAGGAGACTCT

Downstream 100 bases:

>100_bases
GGGCGACCTCGAGCCGTGGATGAAGACGCTTTGGTGAAATGAGACTGGATGGCGAAGATCTGTCAGACTGGGCGTGAATC
TGCAATTGCTGACATTCACC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MGWLADETGLERRAANYVALTPLSHLQRAAHVFADEPALVYGQTRRSYAQYHDRCTRLASALAGMGVAPGDVVATLLPNI
PAQAEAHFGVPACGAVLNTINIRLDVDTVAYIFDHGQAKVVLVDSEFLPLAEAAKERLQGAGPTLIEVPDTEAGHAASGR
YTTYEDLIAHGATDFDWIMPEDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVVSWRLQLRPVYLTIVPLFHCNGW
NHTWMMPVLGGTVVCCRNTTAEAIYNAIADEGVTHFGGAPIVLNMIVNAKDTERRAFDHTVEVFTAGAPPAPATLEKIER
LGFHVTQVYGLTETYGHVTECLWKGDSWDELDQQGRAAIKARQGVAFPMMDHITVMDADMQQVPMNGSDQGEIVMRGNSV
MKGYLKNPEATEEAFAGGYFHSGDIAIQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVVAKPDEKWG
EVPCAFVELKEGADVDEASLIAFARQTLAGFKTPKAVVFCELPKTSTGKIQKFELRKSFAGGS

Sequences:

>Translated_543_residues
MGWLADETGLERRAANYVALTPLSHLQRAAHVFADEPALVYGQTRRSYAQYHDRCTRLASALAGMGVAPGDVVATLLPNI
PAQAEAHFGVPACGAVLNTINIRLDVDTVAYIFDHGQAKVVLVDSEFLPLAEAAKERLQGAGPTLIEVPDTEAGHAASGR
YTTYEDLIAHGATDFDWIMPEDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVVSWRLQLRPVYLTIVPLFHCNGW
NHTWMMPVLGGTVVCCRNTTAEAIYNAIADEGVTHFGGAPIVLNMIVNAKDTERRAFDHTVEVFTAGAPPAPATLEKIER
LGFHVTQVYGLTETYGHVTECLWKGDSWDELDQQGRAAIKARQGVAFPMMDHITVMDADMQQVPMNGSDQGEIVMRGNSV
MKGYLKNPEATEEAFAGGYFHSGDIAIQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVVAKPDEKWG
EVPCAFVELKEGADVDEASLIAFARQTLAGFKTPKAVVFCELPKTSTGKIQKFELRKSFAGGS
>Mature_542_residues
GWLADETGLERRAANYVALTPLSHLQRAAHVFADEPALVYGQTRRSYAQYHDRCTRLASALAGMGVAPGDVVATLLPNIP
AQAEAHFGVPACGAVLNTINIRLDVDTVAYIFDHGQAKVVLVDSEFLPLAEAAKERLQGAGPTLIEVPDTEAGHAASGRY
TTYEDLIAHGATDFDWIMPEDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVVSWRLQLRPVYLTIVPLFHCNGWN
HTWMMPVLGGTVVCCRNTTAEAIYNAIADEGVTHFGGAPIVLNMIVNAKDTERRAFDHTVEVFTAGAPPAPATLEKIERL
GFHVTQVYGLTETYGHVTECLWKGDSWDELDQQGRAAIKARQGVAFPMMDHITVMDADMQQVPMNGSDQGEIVMRGNSVM
KGYLKNPEATEEAFAGGYFHSGDIAIQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVVAKPDEKWGE
VPCAFVELKEGADVDEASLIAFARQTLAGFKTPKAVVFCELPKTSTGKIQKFELRKSFAGGS

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=561, Percent_Identity=27.2727272727273, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI187761345, Length=565, Percent_Identity=26.3716814159292, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI187761343, Length=565, Percent_Identity=26.3716814159292, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI42544132, Length=515, Percent_Identity=26.2135922330097, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI38505220, Length=544, Percent_Identity=25.9191176470588, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI122937307, Length=494, Percent_Identity=25.7085020242915, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=26.1121856866538, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=26.1121856866538, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI58082049, Length=519, Percent_Identity=25.626204238921, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=25.9117082533589, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI13375727, Length=527, Percent_Identity=25.6166982922201, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI28416953, Length=550, Percent_Identity=22.3636363636364, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI42794756, Length=498, Percent_Identity=23.8955823293173, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI42794760, Length=498, Percent_Identity=23.8955823293173, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI42794758, Length=498, Percent_Identity=23.8955823293173, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=548, Percent_Identity=27.1897810218978, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI221142682, Length=524, Percent_Identity=26.5267175572519, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693145, Length=362, Percent_Identity=33.4254143646409, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=27.7358490566038, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1790505, Length=534, Percent_Identity=24.5318352059925, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789201, Length=494, Percent_Identity=23.0769230769231, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=558, Percent_Identity=28.494623655914, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994703, Length=558, Percent_Identity=28.494623655914, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71994690, Length=558, Percent_Identity=28.494623655914, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17558820, Length=356, Percent_Identity=31.4606741573034, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17559526, Length=510, Percent_Identity=26.6666666666667, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=545, Percent_Identity=24.4036697247706, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=545, Percent_Identity=24.954128440367, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=520, Percent_Identity=25.1923076923077, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=357, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17560140, Length=512, Percent_Identity=23.2421875, Blast_Score=108, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=510, Percent_Identity=22.156862745098, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=522, Percent_Identity=23.9463601532567, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17551278, Length=520, Percent_Identity=23.6538461538462, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71983001, Length=572, Percent_Identity=24.1258741258741, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=572, Percent_Identity=24.1258741258741, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=25.4847645429363, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25147511, Length=506, Percent_Identity=23.3201581027668, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=28.4122562674095, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6323182, Length=559, Percent_Identity=25.5813953488372, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=564, Percent_Identity=25, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24581924, Length=552, Percent_Identity=28.4420289855072, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI18859661, Length=529, Percent_Identity=29.3005671077505, Blast_Score=133, Evalue=4e-31,
Organism=Drosophila melanogaster, GI21355181, Length=527, Percent_Identity=25.426944971537, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24667955, Length=547, Percent_Identity=24.4972577696526, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI62472339, Length=547, Percent_Identity=24.4972577696526, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24667959, Length=392, Percent_Identity=26.530612244898, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17933690, Length=543, Percent_Identity=21.9152854511971, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922652, Length=537, Percent_Identity=23.2774674115456, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648260, Length=501, Percent_Identity=23.75249500998, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356947, Length=511, Percent_Identity=21.7221135029354, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130357, Length=360, Percent_Identity=25, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58944; Mature: 58813

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWLADETGLERRAANYVALTPLSHLQRAAHVFADEPALVYGQTRRSYAQYHDRCTRLAS
CCCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
ALAGMGVAPGDVVATLLPNIPAQAEAHFGVPACGAVLNTINIRLDVDTVAYIFDHGQAKV
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEEEEEECCEEEEEEECCCEEE
VLVDSEFLPLAEAAKERLQGAGPTLIEVPDTEAGHAASGRYTTYEDLIAHGATDFDWIMP
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
EDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVVSWRLQLRPVYLTIVPLFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEEEEEEEEEEEEEEEEEEECCC
NHTWMMPVLGGTVVCCRNTTAEAIYNAIADEGVTHFGGAPIVLNMIVNAKDTERRAFDHT
CCEEEEECCCCEEEEECCCHHHHHHHHHHHCCCHHCCCCCEEEEEEECCCCHHHHHHHCE
VEVFTAGAPPAPATLEKIERLGFHVTQVYGLTETYGHVTECLWKGDSWDELDQQGRAAIK
EEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHCHHHHH
ARQGVAFPMMDHITVMDADMQQVPMNGSDQGEIVMRGNSVMKGYLKNPEATEEAFAGGYF
HHCCCCCCHHHCEEEEECCHHHCCCCCCCCCCEEEECCHHHHHHCCCCCHHHHHHCCCCE
HSGDIAIQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVVAKPDEKWG
ECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHC
EVPCAFVELKEGADVDEASLIAFARQTLAGFKTPKAVVFCELPKTSTGKIQKFELRKSFA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEHHHHCC
GGS
CCC
>Mature Secondary Structure 
GWLADETGLERRAANYVALTPLSHLQRAAHVFADEPALVYGQTRRSYAQYHDRCTRLAS
CCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHH
ALAGMGVAPGDVVATLLPNIPAQAEAHFGVPACGAVLNTINIRLDVDTVAYIFDHGQAKV
HHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHEEEEEEEECCEEEEEEECCCEEE
VLVDSEFLPLAEAAKERLQGAGPTLIEVPDTEAGHAASGRYTTYEDLIAHGATDFDWIMP
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
EDEWESLALNYTSGTTGRPKGVVYHHRGAYLMTMGTVVSWRLQLRPVYLTIVPLFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEEECCEEEEEECCEEEEEEEEEEEEEEEEEEEEECCC
NHTWMMPVLGGTVVCCRNTTAEAIYNAIADEGVTHFGGAPIVLNMIVNAKDTERRAFDHT
CCEEEEECCCCEEEEECCCHHHHHHHHHHHCCCHHCCCCCEEEEEEECCCCHHHHHHHCE
VEVFTAGAPPAPATLEKIERLGFHVTQVYGLTETYGHVTECLWKGDSWDELDQQGRAAIK
EEEEECCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHCHHHHH
ARQGVAFPMMDHITVMDADMQQVPMNGSDQGEIVMRGNSVMKGYLKNPEATEEAFAGGYF
HHCCCCCCHHHCEEEEECCHHHCCCCCCCCCCEEEECCHHHHHHCCCCCHHHHHHCCCCE
HSGDIAIQHPDGYIQIADRAKDIIISGGENISSVEVEGVLMGHPDVNLAAVVAKPDEKWG
ECCCEEEECCCCEEEEECCCCEEEEECCCCCCEEEEEEEEECCCCCCEEEEEECCCHHHC
EVPCAFVELKEGADVDEASLIAFARQTLAGFKTPKAVVFCELPKTSTGKIQKFELRKSFA
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEHHHHCC
GGS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]