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The map label for this gene is yfkN [H]

Identifier: 99081389

GI number: 99081389

Start: 1654635

End: 1656215

Strand: Reverse

Name: yfkN [H]

Synonym: TM1040_1548

Alternate gene names: 99081389

Gene position: 1656215-1654635 (Counterclockwise)

Preceding gene: 99081392

Following gene: 99081388

Centisome position: 51.74

GC content: 60.47

Gene sequence:

>1581_bases
ATGATTTCACGTTTTCTGACGTCGGCAGCGGCGCTGGGGCTTACGGCTGGCATGGCCGCGGCCGAGTACAAGCTGACGAT
CTTGCACACCAACGACATCCACAGCCGCATCGAGTCGATCAGCAAATATGATTCCACTTGCGGCGCCGATGACGAGGCCG
AGGGCAAGTGTTTTGGCGGCATCGCCCGCGTCAAGACCATGGTCGACACCAAACGTGCCGAGCTCGACGGCCAGAACGTG
CTTCTGCTGGACGCGGGCGACCCGTTCCAGGGCTCGCTGTTTTACACCACCTACAAGGGGGCAGCCGAAGCCGAGTTCAT
GGAAGACATCGGCTATGACGTAATGGCGGTGGGCAACCACGAATTTGACGACGGACCGGCCGGTCTGCAGCAATTTGTCG
ACACTGTGTCTTTCCCGGTGATTTCCGGCAACCTCGATCTGAGCTCCGAGCCGCTCCTGAAAGGCAAGGTGGGCAACCAT
GTCGTGCTTGAAGTGGGCGGCGAGAAAATCGGCATCATCTCCGCGCTGGCGACAGACACGGTCGAGACCTCCTCGCCGGG
GCCGAATGTGGTGTTTCAGGATGAGATCGACAGCCTGATCGCCGACGTTGAGGCCCTGCAGGCAGAAGGCGTCAACAAGA
TCATCGCGCTGACCCATGTGGGTCTGGCCAAGGATATGGAAATCGCCGCCAAAGTGCCGGGGGTGGATCTCGTCGTGGGT
GGTCATTCGCACACGCTTTTGTCCAACACCTCTGATCGTGCCGCGGGCGCATATCCGACCATGGTGGGCGATGTGCCAGT
GGTGCAGGCCTATGCCTATACCAAGTACCTGGGCGAGCTCACTGTGACCTTTGATGACGAAGGCAATGTCATCTCCGCTG
CGGGCGAGCCGATCCTGCTTGATGCCTCTGTGACGCCGGATGCCGACATGGTCGCGCGCATCAAGGAGATGGGTGCTCCC
ATCGATGAGATGAAAACCCGCGTGGTTGCCGAGACAACCGATGCGGTCGAAGGCTCGCGTGATGTCTGCCGCGCTGGCGA
ATGTGCCATGGGCAACCTCGTCGCGGATGCCATGCTGGCCCGCGTCAAGGATCAGGGTGTGAGCATTGCGATCCAGAACG
GTGGCGGTCTGCGCGCATCGATCGATGCGGGCGAAGTCACCATGGGTGAAGTGCTGAGCGTCCTGCCGTTCCAGAACACG
CTCTCCACCTTTGAGGTCTCCGGCCAGACGATGATTGAGGCCTTGGAAAACGGCGTTGGGCAGATCGAGGACGGCGCAGG
CCGCTTCCCGCAGGTTGCAGGGCTGAAATATGCGTTTGACGCCTCCAAGGAGCCGGGCGCGCGCATTTCCGACGTGATGG
TCATGGAAGGCGAGACCTGGGTTGCGATTGATCCGGCCAAAACCTACGGCGTTGTGTCCAACAACTACGTGCGCAATGGC
GGCGACGGCTACAAGATGTTCGCAGGCGACGACAAGAACGCTTATGACTTTGGCCCCGACCTTGCGGATGTTGTTGCCGA
ATACCTCGCCGAGGTCGGCCCCTACAGCGCCTATACCGACGGCCGCATCACCAAGAAGTAA

Upstream 100 bases:

>100_bases
TCACGTAAGTTTGGTTTTGACTGTTCGGACAAAGATCATGAATATTGATCCCATAACAACCGCCAGTAGAGGCGGCATAA
CGAAGGTGAGGGTTTATCCT

Downstream 100 bases:

>100_bases
CGGCCCCGTCCGCTCCTGAGCCTGTTGGTTTGCGCCGCGCAAAACAACAGGTCAGCGCATGCGGCATTAGGCGGCACTTT
CCGAGACCCCGGCGGTTCGT

Product: 5'-nucleotidase-like

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MISRFLTSAAALGLTAGMAAAEYKLTILHTNDIHSRIESISKYDSTCGADDEAEGKCFGGIARVKTMVDTKRAELDGQNV
LLLDAGDPFQGSLFYTTYKGAAEAEFMEDIGYDVMAVGNHEFDDGPAGLQQFVDTVSFPVISGNLDLSSEPLLKGKVGNH
VVLEVGGEKIGIISALATDTVETSSPGPNVVFQDEIDSLIADVEALQAEGVNKIIALTHVGLAKDMEIAAKVPGVDLVVG
GHSHTLLSNTSDRAAGAYPTMVGDVPVVQAYAYTKYLGELTVTFDDEGNVISAAGEPILLDASVTPDADMVARIKEMGAP
IDEMKTRVVAETTDAVEGSRDVCRAGECAMGNLVADAMLARVKDQGVSIAIQNGGGLRASIDAGEVTMGEVLSVLPFQNT
LSTFEVSGQTMIEALENGVGQIEDGAGRFPQVAGLKYAFDASKEPGARISDVMVMEGETWVAIDPAKTYGVVSNNYVRNG
GDGYKMFAGDDKNAYDFGPDLADVVAEYLAEVGPYSAYTDGRITKK

Sequences:

>Translated_526_residues
MISRFLTSAAALGLTAGMAAAEYKLTILHTNDIHSRIESISKYDSTCGADDEAEGKCFGGIARVKTMVDTKRAELDGQNV
LLLDAGDPFQGSLFYTTYKGAAEAEFMEDIGYDVMAVGNHEFDDGPAGLQQFVDTVSFPVISGNLDLSSEPLLKGKVGNH
VVLEVGGEKIGIISALATDTVETSSPGPNVVFQDEIDSLIADVEALQAEGVNKIIALTHVGLAKDMEIAAKVPGVDLVVG
GHSHTLLSNTSDRAAGAYPTMVGDVPVVQAYAYTKYLGELTVTFDDEGNVISAAGEPILLDASVTPDADMVARIKEMGAP
IDEMKTRVVAETTDAVEGSRDVCRAGECAMGNLVADAMLARVKDQGVSIAIQNGGGLRASIDAGEVTMGEVLSVLPFQNT
LSTFEVSGQTMIEALENGVGQIEDGAGRFPQVAGLKYAFDASKEPGARISDVMVMEGETWVAIDPAKTYGVVSNNYVRNG
GDGYKMFAGDDKNAYDFGPDLADVVAEYLAEVGPYSAYTDGRITKK
>Mature_526_residues
MISRFLTSAAALGLTAGMAAAEYKLTILHTNDIHSRIESISKYDSTCGADDEAEGKCFGGIARVKTMVDTKRAELDGQNV
LLLDAGDPFQGSLFYTTYKGAAEAEFMEDIGYDVMAVGNHEFDDGPAGLQQFVDTVSFPVISGNLDLSSEPLLKGKVGNH
VVLEVGGEKIGIISALATDTVETSSPGPNVVFQDEIDSLIADVEALQAEGVNKIIALTHVGLAKDMEIAAKVPGVDLVVG
GHSHTLLSNTSDRAAGAYPTMVGDVPVVQAYAYTKYLGELTVTFDDEGNVISAAGEPILLDASVTPDADMVARIKEMGAP
IDEMKTRVVAETTDAVEGSRDVCRAGECAMGNLVADAMLARVKDQGVSIAIQNGGGLRASIDAGEVTMGEVLSVLPFQNT
LSTFEVSGQTMIEALENGVGQIEDGAGRFPQVAGLKYAFDASKEPGARISDVMVMEGETWVAIDPAKTYGVVSNNYVRNG
GDGYKMFAGDDKNAYDFGPDLADVVAEYLAEVGPYSAYTDGRITKK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=545, Percent_Identity=40.7339449541284, Blast_Score=343, Evalue=3e-94,
Organism=Escherichia coli, GI1786687, Length=537, Percent_Identity=27.7467411545624, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790658, Length=590, Percent_Identity=22.2033898305085, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922446, Length=529, Percent_Identity=37.9962192816635, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24654424, Length=529, Percent_Identity=37.9962192816635, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI28573524, Length=492, Percent_Identity=37.6016260162602, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI19922444, Length=527, Percent_Identity=37.0018975332068, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24641187, Length=532, Percent_Identity=33.2706766917293, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI221329836, Length=534, Percent_Identity=32.9588014981273, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24652512, Length=495, Percent_Identity=28.0808080808081, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19921980, Length=495, Percent_Identity=28.0808080808081, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI161076508, Length=473, Percent_Identity=28.5412262156448, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 55352; Mature: 55352

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRFLTSAAALGLTAGMAAAEYKLTILHTNDIHSRIESISKYDSTCGADDEAEGKCFGG
CCHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IARVKTMVDTKRAELDGQNVLLLDAGDPFQGSLFYTTYKGAAEAEFMEDIGYDVMAVGNH
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
EFDDGPAGLQQFVDTVSFPVISGNLDLSSEPLLKGKVGNHVVLEVGGEKIGIISALATDT
CCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCEEEEEECCCEEEEEEEHHHCC
VETSSPGPNVVFQDEIDSLIADVEALQAEGVNKIIALTHVGLAKDMEIAAKVPGVDLVVG
CCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCEEEEEC
GHSHTLLSNTSDRAAGAYPTMVGDVPVVQAYAYTKYLGELTVTFDDEGNVISAAGEPILL
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCEEEECCCCEEE
DASVTPDADMVARIKEMGAPIDEMKTRVVAETTDAVEGSRDVCRAGECAMGNLVADAMLA
ECCCCCCHHHHHHHHHHCCCHHHHHHHHHEECHHHCCCCHHHHHCCCCHHHHHHHHHHHH
RVKDQGVSIAIQNGGGLRASIDAGEVTMGEVLSVLPFQNTLSTFEVSGQTMIEALENGVG
HHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCC
QIEDGAGRFPQVAGLKYAFDASKEPGARISDVMVMEGETWVAIDPAKTYGVVSNNYVRNG
CCCCCCCCCCHHCCEEEEECCCCCCCCCCEEEEEECCCEEEEECCCHHCCEECCCEEECC
GDGYKMFAGDDKNAYDFGPDLADVVAEYLAEVGPYSAYTDGRITKK
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECC
>Mature Secondary Structure
MISRFLTSAAALGLTAGMAAAEYKLTILHTNDIHSRIESISKYDSTCGADDEAEGKCFGG
CCHHHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
IARVKTMVDTKRAELDGQNVLLLDAGDPFQGSLFYTTYKGAAEAEFMEDIGYDVMAVGNH
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEECCC
EFDDGPAGLQQFVDTVSFPVISGNLDLSSEPLLKGKVGNHVVLEVGGEKIGIISALATDT
CCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCEEEEEECCCEEEEEEEHHHCC
VETSSPGPNVVFQDEIDSLIADVEALQAEGVNKIIALTHVGLAKDMEIAAKVPGVDLVVG
CCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCEEEEEC
GHSHTLLSNTSDRAAGAYPTMVGDVPVVQAYAYTKYLGELTVTFDDEGNVISAAGEPILL
CCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCEEEECCCCEEE
DASVTPDADMVARIKEMGAPIDEMKTRVVAETTDAVEGSRDVCRAGECAMGNLVADAMLA
ECCCCCCHHHHHHHHHHCCCHHHHHHHHHEECHHHCCCCHHHHHCCCCHHHHHHHHHHHH
RVKDQGVSIAIQNGGGLRASIDAGEVTMGEVLSVLPFQNTLSTFEVSGQTMIEALENGVG
HHHCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCC
QIEDGAGRFPQVAGLKYAFDASKEPGARISDVMVMEGETWVAIDPAKTYGVVSNNYVRNG
CCCCCCCCCCHHCCEEEEECCCCCCCCCCEEEEEECCCEEEEECCCHHCCEECCCEEECC
GDGYKMFAGDDKNAYDFGPDLADVVAEYLAEVGPYSAYTDGRITKK
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]