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The map label for this gene is alsS [H]

Identifier: 99081371

GI number: 99081371

Start: 1633394

End: 1635007

Strand: Direct

Name: alsS [H]

Synonym: TM1040_1530

Alternate gene names: 99081371

Gene position: 1633394-1635007 (Clockwise)

Preceding gene: 99081370

Following gene: 99081372

Centisome position: 51.03

GC content: 64.75

Gene sequence:

>1614_bases
ATGACCCAATCTCTTCGCGCCGCCGATATCCTGGCCCGTCGACTCTACGAGGCCGGATGCCGCCACGCCTTTGGCATGCC
CGGGGGCGAAGTCCTGACACTTGTGGATGCGCTCACCCGCGCTGGGATCACCTTCCACCTCGCCAAACATGAAAACTGCG
CCGGTTTTATGGGCGAAGGCGTGCATCACAGCGATGGGGCGCCCGCAATTCTGGTCGCAACCCTTGGACCCGGCGCGCTC
AATGGGGTCAATGTGGTCGCCAATGCCCATCAGGACCGGGTGCCGATGCTGGTGCTGACAGGCTGCGTCGACGCGGATGA
GGCGCAGACCTACACCCATCAGGTCCTGGATCAACAGGCCGTCTTTGCCCCCATCACCAAGGCCAGTTTCCGTCTGAACG
CCGCTGCAGCCGAAGTGATCGCCGACAAGGCGGTCGCCATCGCAGGCGAGGCCCGTCAAGGACCGGTTCATATCGATGTG
CCCATTTCGGTTGCCAACGCACCGGCCACACCGCGCAACACGCGCCGCGCGCCTGCCGGAGCCTGCGCGCCTGCGGGCGC
TACACTGGCGCAGGCGCAGGACTGGCTGCACGAGGCCAAACGCCCCTTGGCGGTGGTCGGGCTCGATGCGCTTTTGCCCG
GCGGTCCAGACGCCACCCGCGCGTTTCTGGAGGCGCGACAAATCCCCTTTGTCACCAGCTACAAGGCCAAGGGCATCCTG
CCGGAGGATCATCCGCTCTGTCTGGGCGGTGCGGGTCTGTCACCGCTTGCGGATCGCCACTTGCTGCCGCTCATCGAGGC
CAGCGACCTTATTCTCGGGATCGGCTATGACCCGATCGAAATGCGCACCGGCTGGCGCGCGGCCTGGGACGCCACAGAGA
CCCGCGTGATCGACATCACGCCTGAGGCCAACACCCATTACATGCATCAGGCCAGCCTGCCACTGATCGCCGACATTCCT
GCCACGCTCAATGCGCTTGCGGTTGCACCTCAACCCGCACCACCCTGGCCAAACGGGGAAATCGAAAAGGCCAAGACCGC
CCTTGCCGAGGCCTTCCCCCGCAGCGACGACTGGGGGCCTGCGGGCGTCATTGCTGAATGCATCGACACCCTACCCGCCG
ATAGCCTGCTGACCGCAGACAGCGGCGCACACCGTATTCTCCTGAGCCAGATGTGGCCCTGCCAGGAACCGCGCCAGCTG
GTACAATCCTCCGGGCTTTGCACCATGGGCTGCGCAGTGCCACTTGCCATGGGGCGCAAGCTCGCCGAACCCGAACGCAC
CGTGGTGAGCTTTTCAGGGGATGCCGGGTTTCTGATGGTTGCAGGCGAGCTGGCGACCGCGGCAGAACTGGGTCTTGCCC
CAATCTTCGTTGTCTTTGTGGATGCCTCGCTTGCCCTCATCGACCTCAAACAACGCCAGCGCCAGCTCCCCAATGCAGGC
GTGGATTTTGGCCGGCACGACTTTGCCGCCATCGGACGCGCCTTTGGCGGCGAAGGCCATCGGGTGCAGAACCGAACGGA
GCTGCGCGCCGCGCTGGAGGCGGCTCAATCGGCCTCCACCTTCACCGTGATCGCTGCGGAAATCGACAAGGAGTCCTATG
ATGGCCGCATCTGA

Upstream 100 bases:

>100_bases
CTGACGGCCGTCGCGGCGCGGCTCGACGACGTCTTTGCCCAGATTGCTGCCGCAGCGCTGCAGAATTAATCCCCTATTTC
CAAATCAATACGGACCACAC

Downstream 100 bases:

>100_bases
TACCACCGCCCCCGCGCGTTTGCCCCACGGTGCCCTCAAAGGCGTAAAAGTGCTGGATCTCTCGCGCATCCTGGCTGGCC
CAACCGCAACGCAACTTTTG

Product: thiamine pyrophosphate enzyme-like TPP-binding

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTQSLRAADILARRLYEAGCRHAFGMPGGEVLTLVDALTRAGITFHLAKHENCAGFMGEGVHHSDGAPAILVATLGPGAL
NGVNVVANAHQDRVPMLVLTGCVDADEAQTYTHQVLDQQAVFAPITKASFRLNAAAAEVIADKAVAIAGEARQGPVHIDV
PISVANAPATPRNTRRAPAGACAPAGATLAQAQDWLHEAKRPLAVVGLDALLPGGPDATRAFLEARQIPFVTSYKAKGIL
PEDHPLCLGGAGLSPLADRHLLPLIEASDLILGIGYDPIEMRTGWRAAWDATETRVIDITPEANTHYMHQASLPLIADIP
ATLNALAVAPQPAPPWPNGEIEKAKTALAEAFPRSDDWGPAGVIAECIDTLPADSLLTADSGAHRILLSQMWPCQEPRQL
VQSSGLCTMGCAVPLAMGRKLAEPERTVVSFSGDAGFLMVAGELATAAELGLAPIFVVFVDASLALIDLKQRQRQLPNAG
VDFGRHDFAAIGRAFGGEGHRVQNRTELRAALEAAQSASTFTVIAAEIDKESYDGRI

Sequences:

>Translated_537_residues
MTQSLRAADILARRLYEAGCRHAFGMPGGEVLTLVDALTRAGITFHLAKHENCAGFMGEGVHHSDGAPAILVATLGPGAL
NGVNVVANAHQDRVPMLVLTGCVDADEAQTYTHQVLDQQAVFAPITKASFRLNAAAAEVIADKAVAIAGEARQGPVHIDV
PISVANAPATPRNTRRAPAGACAPAGATLAQAQDWLHEAKRPLAVVGLDALLPGGPDATRAFLEARQIPFVTSYKAKGIL
PEDHPLCLGGAGLSPLADRHLLPLIEASDLILGIGYDPIEMRTGWRAAWDATETRVIDITPEANTHYMHQASLPLIADIP
ATLNALAVAPQPAPPWPNGEIEKAKTALAEAFPRSDDWGPAGVIAECIDTLPADSLLTADSGAHRILLSQMWPCQEPRQL
VQSSGLCTMGCAVPLAMGRKLAEPERTVVSFSGDAGFLMVAGELATAAELGLAPIFVVFVDASLALIDLKQRQRQLPNAG
VDFGRHDFAAIGRAFGGEGHRVQNRTELRAALEAAQSASTFTVIAAEIDKESYDGRI
>Mature_536_residues
TQSLRAADILARRLYEAGCRHAFGMPGGEVLTLVDALTRAGITFHLAKHENCAGFMGEGVHHSDGAPAILVATLGPGALN
GVNVVANAHQDRVPMLVLTGCVDADEAQTYTHQVLDQQAVFAPITKASFRLNAAAAEVIADKAVAIAGEARQGPVHIDVP
ISVANAPATPRNTRRAPAGACAPAGATLAQAQDWLHEAKRPLAVVGLDALLPGGPDATRAFLEARQIPFVTSYKAKGILP
EDHPLCLGGAGLSPLADRHLLPLIEASDLILGIGYDPIEMRTGWRAAWDATETRVIDITPEANTHYMHQASLPLIADIPA
TLNALAVAPQPAPPWPNGEIEKAKTALAEAFPRSDDWGPAGVIAECIDTLPADSLLTADSGAHRILLSQMWPCQEPRQLV
QSSGLCTMGCAVPLAMGRKLAEPERTVVSFSGDAGFLMVAGELATAAELGLAPIFVVFVDASLALIDLKQRQRQLPNAGV
DFGRHDFAAIGRAFGGEGHRVQNRTELRAALEAAQSASTFTVIAAEIDKESYDGRI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=549, Percent_Identity=21.6757741347905, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1790104, Length=546, Percent_Identity=29.4871794871795, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081685, Length=542, Percent_Identity=28.2287822878229, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI1786717, Length=486, Percent_Identity=26.1316872427983, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=25.3597122302158, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787096, Length=546, Percent_Identity=24.3589743589744, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=26.1022927689594, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=26.4550264550265, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=25.7391304347826, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320816, Length=554, Percent_Identity=22.3826714801444, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=470, Percent_Identity=22.7659574468085, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=24.6861924686192, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56535; Mature: 56404

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSLRAADILARRLYEAGCRHAFGMPGGEVLTLVDALTRAGITFHLAKHENCAGFMGEG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VHHSDGAPAILVATLGPGALNGVNVVANAHQDRVPMLVLTGCVDADEAQTYTHQVLDQQA
CCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VFAPITKASFRLNAAAAEVIADKAVAIAGEARQGPVHIDVPISVANAPATPRNTRRAPAG
HHHCCCHHHEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
ACAPAGATLAQAQDWLHEAKRPLAVVGLDALLPGGPDATRAFLEARQIPFVTSYKAKGIL
CCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCC
PEDHPLCLGGAGLSPLADRHLLPLIEASDLILGIGYDPIEMRTGWRAAWDATETRVIDIT
CCCCCEEEECCCCCCHHCCCCCCEECCCCEEEECCCCCHHHCCCCCEECCCCCCEEEEEC
PEANTHYMHQASLPLIADIPATLNALAVAPQPAPPWPNGEIEKAKTALAEAFPRSDDWGP
CCCCCCEEEECCCCEEECCCHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
AGVIAECIDTLPADSLLTADSGAHRILLSQMWPCQEPRQLVQSSGLCTMGCAVPLAMGRK
HHHHHHHHHHCCCCCEEECCCCCCEEHHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHCHH
LAEPERTVVSFSGDAGFLMVAGELATAAELGLAPIFVVFVDASLALIDLKQRQRQLPNAG
CCCCCCEEEEECCCCCEEEEECCHHHHHHHCCHHEEEEHHCCHHHHHHHHHHHHHCCCCC
VDFGRHDFAAIGRAFGGEGHRVQNRTELRAALEAAQSASTFTVIAAEIDKESYDGRI
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TQSLRAADILARRLYEAGCRHAFGMPGGEVLTLVDALTRAGITFHLAKHENCAGFMGEG
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
VHHSDGAPAILVATLGPGALNGVNVVANAHQDRVPMLVLTGCVDADEAQTYTHQVLDQQA
CCCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
VFAPITKASFRLNAAAAEVIADKAVAIAGEARQGPVHIDVPISVANAPATPRNTRRAPAG
HHHCCCHHHEEEHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCC
ACAPAGATLAQAQDWLHEAKRPLAVVGLDALLPGGPDATRAFLEARQIPFVTSYKAKGIL
CCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCC
PEDHPLCLGGAGLSPLADRHLLPLIEASDLILGIGYDPIEMRTGWRAAWDATETRVIDIT
CCCCCEEEECCCCCCHHCCCCCCEECCCCEEEECCCCCHHHCCCCCEECCCCCCEEEEEC
PEANTHYMHQASLPLIADIPATLNALAVAPQPAPPWPNGEIEKAKTALAEAFPRSDDWGP
CCCCCCEEEECCCCEEECCCHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCH
AGVIAECIDTLPADSLLTADSGAHRILLSQMWPCQEPRQLVQSSGLCTMGCAVPLAMGRK
HHHHHHHHHHCCCCCEEECCCCCCEEHHHHCCCCHHHHHHHHHCCCEEEHHHHHHHHCHH
LAEPERTVVSFSGDAGFLMVAGELATAAELGLAPIFVVFVDASLALIDLKQRQRQLPNAG
CCCCCCEEEEECCCCCEEEEECCHHHHHHHCCHHEEEEHHCCHHHHHHHHHHHHHCCCCC
VDFGRHDFAAIGRAFGGEGHRVQNRTELRAALEAAQSASTFTVIAAEIDKESYDGRI
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]