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The map label for this gene is dctB [H]

Identifier: 99081303

GI number: 99081303

Start: 1562751

End: 1564553

Strand: Reverse

Name: dctB [H]

Synonym: TM1040_1462

Alternate gene names: 99081303

Gene position: 1564553-1562751 (Counterclockwise)

Preceding gene: 99081304

Following gene: 99081302

Centisome position: 48.88

GC content: 62.12

Gene sequence:

>1803_bases
ATGACTGCCTCTGAGGAGACTCCCGGCGCAAAGCCGCCTGCGCCTTCTGAACGTCGTGCTGAGCGCCCGACGTTGAGGTC
GCGCACGATCTCCTGGCGCGTGCGCCTGGCACTTGTGGCCTTCATGGCCATCGCAGGGGCGACCGTCTGGTTCACCAACT
CCACGTTGACCCACAGTTTTACCGAGACCACACGCAATCGGGCGGAACTGCGGCTTGCGCTTTATTCAGGCAACCTCGTC
AGCGAATTGCGCCGCCACGCGATTGTGCCGCAGCTTCTGTCGCGCGACCAGGCGCTGATCTCGGCGCTGCAGAGCCAGGA
TTTTTCGCTTTCGACCCAGCGCCTCATTTCCTTTGTTGATGAAATCGGTGCGGCCTCCATAACCTTGATGGACCGAGATG
GGCGCACGGTTGCGGCCACGGATCGTGTTCGACTCGGTGAAATCTATCGCAATGCGCCCTTCTTTGTTGAGGCAATCCGG
TCGAACGCCACCGTGTTTTCGGTGATCCCGCGCGAGGTGCGGGGGTATCAATTCACGTATTCGCGCCGTCTCGTCGACAG
CAGCGGCACGCTTGGCGTCATTATGGTCGAGGTCGATCTGCAAAAGTTCGAACGCGCCTGGGCGGGCATTTCCGACGCCG
TGATGGTGGCCGACAGCACCGGCACCGTTGTGCTCGCCACAGAGGCGCGCTGGCGGGGGCTCTCGGAAGGGGAGGCCCTG
AAGGAGCAAACACCGGAGGGTGCGATCCAGCGTGCCATCCGCGCGACTGCGGATTGGGCTGCCCTGCCCACCGATGTCTA
CCTGCAAGGTGAGGCGGTGATGCGGCTCGAGACTCGGGTGCCGTTCCAGGGGTGGCGGATGATGTCCTACACCACCTATG
ATTCGATCCGCGAACAGGTCAACGCGGTGATTGCGCTCGAAATCATGGGGTTTGCGATCCTGCTTGCGTTGGCGTTTTAC
GCGTTGAGCCGCAAGACGGCGCTGCGGATGGCGCTGTTTCAGCGCGAGTCGGCGGAGCTTCGCGTGTTGAATGCGCGCCT
GCAGCGGGAAATTGCCGAGCGTGAACGGGTGCAAAAGACCCTCGCTGTGGCTGAACAAAGCCTTGCGCAGAGCTCCAAAC
TGGCCGCGCTCGGAGAGATGTCTGCCGCCGTGAGCCATGAGCTCAACCAGCCGCTGGCGGCGATGAAAACCTACCTCGCG
GGTGCGCGCCTGCTCCTGCAACGTAACCGCCCCGACGAGGCGCTGTCGTCTTTTGGGCGCATCGACGACCTCATTGAACG
CATGGGGGCGATCACGCGGCAGCTGAAATCCTATGCGCGCAAGGGTGGAGACGCGTTCAGCCCAGTAAACATGGGCGATG
CGCTCGCCTCTTCGCTGTCGATGATGGAGCCGCAACTGCGCCAGCGCCACGTCCAGATCACCCGTATCCTGCCGGATGAG
CCGGTCTTTGTGATGGCGGATCGGATGCGACTGGAGCAGGTTATGGTGAACCTCTTGCGCAATGCGCTGGATGCCACCAA
ATCTGTTGAGGAACCCGAGGTTGAGATCATCCTGGCCGCCGGTGAAACTGCGACCCTCACGGTCCGCGACAATGGCGACG
GCATTCAGGATTTCGACAGCCTGTTCGAGCCGTTTTACACCACCAAGCAACCGGGTGACGGTGTCGGACTTGGGCTTGCT
ATCTCCTCGGGGATCGTGAACGACCTAGGCGGTCGCCTCACGGCCCGCAATTCCGATGCGGGCGGCGCGGTGTTTGAAAT
GCAACTGCCGATCCTGATGGACGGCATCGAGGCCGCCGAATAG

Upstream 100 bases:

>100_bases
CCATGGCGGCACCGACGGTGTGGCGATGTTCCGCGCATTGTCCGGACTTCTCACGGACGCCTGACTTGAGCCTCAGAGGC
GGTAAACCTAGTGTTGCGTC

Downstream 100 bases:

>100_bases
GCGGCACGACCTGGAGGGCAACTTATGGCACAGGCCATGAAAATTGCGATTGTGGATGACGAACAGGACATGCGTCAGTC
GATCAGCCAATGGCTGGCAC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTASEETPGAKPPAPSERRAERPTLRSRTISWRVRLALVAFMAIAGATVWFTNSTLTHSFTETTRNRAELRLALYSGNLV
SELRRHAIVPQLLSRDQALISALQSQDFSLSTQRLISFVDEIGAASITLMDRDGRTVAATDRVRLGEIYRNAPFFVEAIR
SNATVFSVIPREVRGYQFTYSRRLVDSSGTLGVIMVEVDLQKFERAWAGISDAVMVADSTGTVVLATEARWRGLSEGEAL
KEQTPEGAIQRAIRATADWAALPTDVYLQGEAVMRLETRVPFQGWRMMSYTTYDSIREQVNAVIALEIMGFAILLALAFY
ALSRKTALRMALFQRESAELRVLNARLQREIAERERVQKTLAVAEQSLAQSSKLAALGEMSAAVSHELNQPLAAMKTYLA
GARLLLQRNRPDEALSSFGRIDDLIERMGAITRQLKSYARKGGDAFSPVNMGDALASSLSMMEPQLRQRHVQITRILPDE
PVFVMADRMRLEQVMVNLLRNALDATKSVEEPEVEIILAAGETATLTVRDNGDGIQDFDSLFEPFYTTKQPGDGVGLGLA
ISSGIVNDLGGRLTARNSDAGGAVFEMQLPILMDGIEAAE

Sequences:

>Translated_600_residues
MTASEETPGAKPPAPSERRAERPTLRSRTISWRVRLALVAFMAIAGATVWFTNSTLTHSFTETTRNRAELRLALYSGNLV
SELRRHAIVPQLLSRDQALISALQSQDFSLSTQRLISFVDEIGAASITLMDRDGRTVAATDRVRLGEIYRNAPFFVEAIR
SNATVFSVIPREVRGYQFTYSRRLVDSSGTLGVIMVEVDLQKFERAWAGISDAVMVADSTGTVVLATEARWRGLSEGEAL
KEQTPEGAIQRAIRATADWAALPTDVYLQGEAVMRLETRVPFQGWRMMSYTTYDSIREQVNAVIALEIMGFAILLALAFY
ALSRKTALRMALFQRESAELRVLNARLQREIAERERVQKTLAVAEQSLAQSSKLAALGEMSAAVSHELNQPLAAMKTYLA
GARLLLQRNRPDEALSSFGRIDDLIERMGAITRQLKSYARKGGDAFSPVNMGDALASSLSMMEPQLRQRHVQITRILPDE
PVFVMADRMRLEQVMVNLLRNALDATKSVEEPEVEIILAAGETATLTVRDNGDGIQDFDSLFEPFYTTKQPGDGVGLGLA
ISSGIVNDLGGRLTARNSDAGGAVFEMQLPILMDGIEAAE
>Mature_599_residues
TASEETPGAKPPAPSERRAERPTLRSRTISWRVRLALVAFMAIAGATVWFTNSTLTHSFTETTRNRAELRLALYSGNLVS
ELRRHAIVPQLLSRDQALISALQSQDFSLSTQRLISFVDEIGAASITLMDRDGRTVAATDRVRLGEIYRNAPFFVEAIRS
NATVFSVIPREVRGYQFTYSRRLVDSSGTLGVIMVEVDLQKFERAWAGISDAVMVADSTGTVVLATEARWRGLSEGEALK
EQTPEGAIQRAIRATADWAALPTDVYLQGEAVMRLETRVPFQGWRMMSYTTYDSIREQVNAVIALEIMGFAILLALAFYA
LSRKTALRMALFQRESAELRVLNARLQREIAERERVQKTLAVAEQSLAQSSKLAALGEMSAAVSHELNQPLAAMKTYLAG
ARLLLQRNRPDEALSSFGRIDDLIERMGAITRQLKSYARKGGDAFSPVNMGDALASSLSMMEPQLRQRHVQITRILPDEP
VFVMADRMRLEQVMVNLLRNALDATKSVEEPEVEIILAAGETATLTVRDNGDGIQDFDSLFEPFYTTKQPGDGVGLGLAI
SSGIVNDLGGRLTARNSDAGGAVFEMQLPILMDGIEAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=230, Percent_Identity=28.695652173913, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=26.7241379310345, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=267, Percent_Identity=31.0861423220974, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=317, Percent_Identity=25.5520504731861, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=29.5652173913043, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786912, Length=247, Percent_Identity=29.9595141700405, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787894, Length=252, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=221, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI48994928, Length=254, Percent_Identity=26.3779527559055, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66125; Mature: 65993

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASEETPGAKPPAPSERRAERPTLRSRTISWRVRLALVAFMAIAGATVWFTNSTLTHSF
CCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHH
TETTRNRAELRLALYSGNLVSELRRHAIVPQLLSRDQALISALQSQDFSLSTQRLISFVD
HHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHH
EIGAASITLMDRDGRTVAATDRVRLGEIYRNAPFFVEAIRSNATVFSVIPREVRGYQFTY
HCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCHHHHHHHCCCEEEEECHHHHCCEEEEE
SRRLVDSSGTLGVIMVEVDLQKFERAWAGISDAVMVADSTGTVVLATEARWRGLSEGEAL
HHHEECCCCCEEEEEEEECHHHHHHHHCCCCCEEEEECCCCCEEEEECHHHCCCCCCHHH
KEQTPEGAIQRAIRATADWAALPTDVYLQGEAVMRLETRVPFQGWRMMSYTTYDSIREQV
HHCCCHHHHHHHHHHHCCHHCCCCCEEECCCEEEEEEECCCCCCCEEEEECHHHHHHHHH
NAVIALEIMGFAILLALAFYALSRKTALRMALFQRESAELRVLNARLQREIAERERVQKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAVAEQSLAQSSKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRNRPDEALSSFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IDDLIERMGAITRQLKSYARKGGDAFSPVNMGDALASSLSMMEPQLRQRHVQITRILPDE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHEEHEEECCCC
PVFVMADRMRLEQVMVNLLRNALDATKSVEEPEVEIILAAGETATLTVRDNGDGIQDFDS
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHH
LFEPFYTTKQPGDGVGLGLAISSGIVNDLGGRLTARNSDAGGAVFEMQLPILMDGIEAAE
HHHHHCCCCCCCCCCEEHHHHHCCHHHHHCCEEEECCCCCCCEEEEEECCHHHCCCCCCC
>Mature Secondary Structure 
TASEETPGAKPPAPSERRAERPTLRSRTISWRVRLALVAFMAIAGATVWFTNSTLTHSF
CCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHH
TETTRNRAELRLALYSGNLVSELRRHAIVPQLLSRDQALISALQSQDFSLSTQRLISFVD
HHHHCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHH
EIGAASITLMDRDGRTVAATDRVRLGEIYRNAPFFVEAIRSNATVFSVIPREVRGYQFTY
HCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCHHHHHHHCCCEEEEECHHHHCCEEEEE
SRRLVDSSGTLGVIMVEVDLQKFERAWAGISDAVMVADSTGTVVLATEARWRGLSEGEAL
HHHEECCCCCEEEEEEEECHHHHHHHHCCCCCEEEEECCCCCEEEEECHHHCCCCCCHHH
KEQTPEGAIQRAIRATADWAALPTDVYLQGEAVMRLETRVPFQGWRMMSYTTYDSIREQV
HHCCCHHHHHHHHHHHCCHHCCCCCEEECCCEEEEEEECCCCCCCEEEEECHHHHHHHHH
NAVIALEIMGFAILLALAFYALSRKTALRMALFQRESAELRVLNARLQREIAERERVQKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LAVAEQSLAQSSKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLQRNRPDEALSSFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IDDLIERMGAITRQLKSYARKGGDAFSPVNMGDALASSLSMMEPQLRQRHVQITRILPDE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHEEHEEECCCC
PVFVMADRMRLEQVMVNLLRNALDATKSVEEPEVEIILAAGETATLTVRDNGDGIQDFDS
CEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCEEEEEEECCCCCHHHHHH
LFEPFYTTKQPGDGVGLGLAISSGIVNDLGGRLTARNSDAGGAVFEMQLPILMDGIEAAE
HHHHHCCCCCCCCCCEEHHHHHCCHHHHHCCEEEECCCCCCCEEEEEECCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]