Click here to switch to the map view.

The map label for this gene is yomI [H]

Identifier: 99081265

GI number: 99081265

Start: 1520328

End: 1520903

Strand: Reverse

Name: yomI [H]

Synonym: TM1040_1424

Alternate gene names: 99081265

Gene position: 1520903-1520328 (Counterclockwise)

Preceding gene: 99081267

Following gene: 99081264

Centisome position: 47.51

GC content: 60.42

Gene sequence:

>576_bases
ATGCGACGTATTTCGACTGCTGCTCTCATCGTGACCTGCCTTGCTACACCTGTCGTGGCTAGTGATCTGTTCAGCAACAG
CTCCAAGCGTAAGGTGTTTGCTGCCCAGACCAAGTTTCTGGATACCCGCGGGGCGACCCAATATCGCGGCTCGGAGCGGT
TGAAACCCGACAGTCTTCAAGATGACGTGCTGCCGGTCTATCGCGGCAGCTATCGCGGGGAGTATCTGGAGGTGGCGCGC
AGCGCTGCGCGTGCAAACGGTGTGCCAGAGGATCTCTTTTTGCGATTGGTGCAGCAGGAGAGCAACTGGAACCCCACCGC
CGAGTCCCACAAGGGCGCGCTTGGTTTGGCGCAGTTGATGCCGCAGACCGCCCGTACTCTGGGCGTGGATCCGTTGGTCC
CGGCTGAAAACCTGAGGGGCGGCGCCAAGTATCTGGCGCAGCAGTACCGCAAGTTCGGCTCTTGGCGACTTGCGCTGGCG
GCTTATAACGCTGGACCTGAGGCGGTCACACGCCATGGCGGCGTGCCGCCCTACAAGGAAACCCAAAATTACGTGCGCAA
GATCCTCGGTAGCTAA

Upstream 100 bases:

>100_bases
TTCGCAACTGGGAGGACACTTCTGCGGTGCTTATCTTTCATTTTAAGGATTGAGGCGCTATATTCCCGCCATCTGAACCG
AAGAGTTTTCGATTTCACAC

Downstream 100 bases:

>100_bases
TCGTGCCTTTGGAGTTGGTTGTGGCGGGTTTCTGTCCGTCCCTTGACGGGGGCGTTAATACAATTTTCAGCATCGTTAAC
CAATTTGCCGCGCCCCTATC

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MRRISTAALIVTCLATPVVASDLFSNSSKRKVFAAQTKFLDTRGATQYRGSERLKPDSLQDDVLPVYRGSYRGEYLEVAR
SAARANGVPEDLFLRLVQQESNWNPTAESHKGALGLAQLMPQTARTLGVDPLVPAENLRGGAKYLAQQYRKFGSWRLALA
AYNAGPEAVTRHGGVPPYKETQNYVRKILGS

Sequences:

>Translated_191_residues
MRRISTAALIVTCLATPVVASDLFSNSSKRKVFAAQTKFLDTRGATQYRGSERLKPDSLQDDVLPVYRGSYRGEYLEVAR
SAARANGVPEDLFLRLVQQESNWNPTAESHKGALGLAQLMPQTARTLGVDPLVPAENLRGGAKYLAQQYRKFGSWRLALA
AYNAGPEAVTRHGGVPPYKETQNYVRKILGS
>Mature_191_residues
MRRISTAALIVTCLATPVVASDLFSNSSKRKVFAAQTKFLDTRGATQYRGSERLKPDSLQDDVLPVYRGSYRGEYLEVAR
SAARANGVPEDLFLRLVQQESNWNPTAESHKGALGLAQLMPQTARTLGVDPLVPAENLRGGAKYLAQQYRKFGSWRLALA
AYNAGPEAVTRHGGVPPYKETQNYVRKILGS

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 10 TPR repeats [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR008258
- InterPro:   IPR016047
- InterPro:   IPR010090
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01551 Peptidase_M23; PF10145 PhageMin_Tail; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 20998; Mature: 20998

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRISTAALIVTCLATPVVASDLFSNSSKRKVFAAQTKFLDTRGATQYRGSERLKPDSLQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DDVLPVYRGSYRGEYLEVARSAARANGVPEDLFLRLVQQESNWNPTAESHKGALGLAQLM
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHH
PQTARTLGVDPLVPAENLRGGAKYLAQQYRKFGSWRLALAAYNAGPEAVTRHGGVPPYKE
HHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCCCCHHH
TQNYVRKILGS
HHHHHHHHHCC
>Mature Secondary Structure
MRRISTAALIVTCLATPVVASDLFSNSSKRKVFAAQTKFLDTRGATQYRGSERLKPDSLQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DDVLPVYRGSYRGEYLEVARSAARANGVPEDLFLRLVQQESNWNPTAESHKGALGLAQLM
CCCHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHH
PQTARTLGVDPLVPAENLRGGAKYLAQQYRKFGSWRLALAAYNAGPEAVTRHGGVPPYKE
HHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHCCCCCCHHH
TQNYVRKILGS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]