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The map label for this gene is bclA [H]

Identifier: 99081187

GI number: 99081187

Start: 1433650

End: 1435158

Strand: Direct

Name: bclA [H]

Synonym: TM1040_1346

Alternate gene names: 99081187

Gene position: 1433650-1435158 (Clockwise)

Preceding gene: 99081185

Following gene: 99081188

Centisome position: 44.79

GC content: 60.3

Gene sequence:

>1509_bases
ATGCTTTCCATTTTTGACACCGGCGCACCGCCTGCCTGCCCCCCAGCTTTCAATCTTGCGGCGCATGTCTTGCGCCACGC
CGCGAACCGTGCCGGTCACCCCGCCCTGGTGATTGCAGGACCGGAGCAGGATCAGATCTGGACCTATGCTGCCTTTGAAC
GCGCGGTGCGGGGAACCGCACGGGGCTTTCTCGAATCCGGATTTGAGCCCGGCGATATCCTCTTGATGCGATTGGGAAAT
ACCGTCGAATTCCCGATTACGTTTCTCGCCGCGATCCTGGTCGGTATTGTGCCAGTGCCGACATCTTCGCAGCTGACCAA
ACGCGAGGTCGCGCGGATGGTGGAGGATCTATCCCCCGCAGGGATCGTCAGCGCGCCTGACATCGCCACCCATCCGCACT
CGCGCGCGGTCTCTCTTGATAGGCTGCGCGCCATGCAGTCCTGCCCACCAGCCGACGCCGTGCTCGGTGATCCGGATCGG
CTCGCCTATATCGTCTATACCTCTGGCACTGGCGGATCTCCGCGCGCGGTGCAACATGCCCATCGGGCTATCTGGGCCCG
GCAGATGATGATCCGCGACTGGTATGACCTGAGCCGAAATGATCGCCTGCTGCATGCAGGAGCCTTCAACTGGACCTTTA
CCCTTGGCACCGGGATCATGGATCCGCTGTCCGTCGGTGCAACCGCCATCATTCCATCTGCCGACACGGATCTTTTGGAC
CTGCCGCGGGTATTGACGCGCACTCAGGCGACGATCTTTGCCGCCGTACCAGGTGTTTATAGAAAGATTCTGAAGTGCGA
CACCCTGCCCGTTCTGCCTGACCTCAGACATGGGCTTGCAGCGGGTGAGGCTCTGAGTGGCGATATCCGCGACGGCTGGA
AAGATCGAATTGGCACCCCGATCTTTGAGGCCTTTGGCATGTCTGAATGCTCCACCTTTATCTCCTCGGCCCCGCATCGC
CCTTCTGTCGCACAGAGCATGGGCGCGCCACAACGCGGCCGCAGAATTGCGATCATCGGCGAGGATGGCCCTGCTCCAAT
CGGCGAAGAAGGGATCATCGCGGTTCACCGATCTGATCCCGGTTTGATGCTCGGCTACCTCAATGCCCCCGAGGAGACTC
GCGCGCGCTTTGACGGTGAGTGGTTTTTGACCGGGGATCGTGGCATCATGCGGGCGGATGGCCAGATCATCTATTGCGGG
CGCAACGACGATATGATGAATGCAGGGGGCTTTCGCGTCTCCCCGCTCGAGATCGAAACAGTGTTTGCGACCCATCCCGG
TCTGTCTCAATTTGCGGCGGCTGAGGTGGAGGTGAAACCCGGCGTCCACATCATCGTGGGCGTTTTCAAGGGTCGCGAGG
ATGTGACCGAAATGGCGCTACAGGCTTTTGCACAAGAGCGTCTGGCCGCGTACAAACGCCCAAAGACTTACAAGCGCCTG
GCGGAGTTTCCAACCAACCCGAATGGAAAACTCTTGCGCCGCGCCCTGCCCGATCTGTTTAAGGACTGA

Upstream 100 bases:

>100_bases
TCTTCGGGTCGCGTTCCTGCATAGCAACCCTATAAGTCTGCACGCAGGGCCTTGCAACGCCGACTTGCTCGAATGCGCAC
CTCAGGATAGGCATTTCACC

Downstream 100 bases:

>100_bases
CCCATGACTGAACCGCTTGCGCAAGTGAAACTCGATATTCTGTCGGATCCGATCTGCCCCTGGTGCTATATCGGCAAGAC
CCATCTGGACCGCGCTCTGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLSIFDTGAPPACPPAFNLAAHVLRHAANRAGHPALVIAGPEQDQIWTYAAFERAVRGTARGFLESGFEPGDILLMRLGN
TVEFPITFLAAILVGIVPVPTSSQLTKREVARMVEDLSPAGIVSAPDIATHPHSRAVSLDRLRAMQSCPPADAVLGDPDR
LAYIVYTSGTGGSPRAVQHAHRAIWARQMMIRDWYDLSRNDRLLHAGAFNWTFTLGTGIMDPLSVGATAIIPSADTDLLD
LPRVLTRTQATIFAAVPGVYRKILKCDTLPVLPDLRHGLAAGEALSGDIRDGWKDRIGTPIFEAFGMSECSTFISSAPHR
PSVAQSMGAPQRGRRIAIIGEDGPAPIGEEGIIAVHRSDPGLMLGYLNAPEETRARFDGEWFLTGDRGIMRADGQIIYCG
RNDDMMNAGGFRVSPLEIETVFATHPGLSQFAAAEVEVKPGVHIIVGVFKGREDVTEMALQAFAQERLAAYKRPKTYKRL
AEFPTNPNGKLLRRALPDLFKD

Sequences:

>Translated_502_residues
MLSIFDTGAPPACPPAFNLAAHVLRHAANRAGHPALVIAGPEQDQIWTYAAFERAVRGTARGFLESGFEPGDILLMRLGN
TVEFPITFLAAILVGIVPVPTSSQLTKREVARMVEDLSPAGIVSAPDIATHPHSRAVSLDRLRAMQSCPPADAVLGDPDR
LAYIVYTSGTGGSPRAVQHAHRAIWARQMMIRDWYDLSRNDRLLHAGAFNWTFTLGTGIMDPLSVGATAIIPSADTDLLD
LPRVLTRTQATIFAAVPGVYRKILKCDTLPVLPDLRHGLAAGEALSGDIRDGWKDRIGTPIFEAFGMSECSTFISSAPHR
PSVAQSMGAPQRGRRIAIIGEDGPAPIGEEGIIAVHRSDPGLMLGYLNAPEETRARFDGEWFLTGDRGIMRADGQIIYCG
RNDDMMNAGGFRVSPLEIETVFATHPGLSQFAAAEVEVKPGVHIIVGVFKGREDVTEMALQAFAQERLAAYKRPKTYKRL
AEFPTNPNGKLLRRALPDLFKD
>Mature_502_residues
MLSIFDTGAPPACPPAFNLAAHVLRHAANRAGHPALVIAGPEQDQIWTYAAFERAVRGTARGFLESGFEPGDILLMRLGN
TVEFPITFLAAILVGIVPVPTSSQLTKREVARMVEDLSPAGIVSAPDIATHPHSRAVSLDRLRAMQSCPPADAVLGDPDR
LAYIVYTSGTGGSPRAVQHAHRAIWARQMMIRDWYDLSRNDRLLHAGAFNWTFTLGTGIMDPLSVGATAIIPSADTDLLD
LPRVLTRTQATIFAAVPGVYRKILKCDTLPVLPDLRHGLAAGEALSGDIRDGWKDRIGTPIFEAFGMSECSTFISSAPHR
PSVAQSMGAPQRGRRIAIIGEDGPAPIGEEGIIAVHRSDPGLMLGYLNAPEETRARFDGEWFLTGDRGIMRADGQIIYCG
RNDDMMNAGGFRVSPLEIETVFATHPGLSQFAAAEVEVKPGVHIIVGVFKGREDVTEMALQAFAQERLAAYKRPKTYKRL
AEFPTNPNGKLLRRALPDLFKD

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=535, Percent_Identity=25.0467289719626, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI42544132, Length=527, Percent_Identity=23.5294117647059, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI122937307, Length=528, Percent_Identity=26.1363636363636, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI115511026, Length=553, Percent_Identity=25.6781193490054, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI58082049, Length=533, Percent_Identity=24.390243902439, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI157311624, Length=533, Percent_Identity=24.390243902439, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI157311622, Length=533, Percent_Identity=24.390243902439, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI187761345, Length=384, Percent_Identity=27.0833333333333, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI187761343, Length=384, Percent_Identity=27.0833333333333, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI195947378, Length=435, Percent_Identity=27.3563218390805, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI42544134, Length=337, Percent_Identity=22.2551928783383, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI156151445, Length=524, Percent_Identity=22.9007633587786, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=23.7827715355805, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI145693145, Length=504, Percent_Identity=22.8174603174603, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786801, Length=517, Percent_Identity=24.3713733075435, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786810, Length=504, Percent_Identity=22.4206349206349, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789201, Length=359, Percent_Identity=23.9554317548747, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI221142682, Length=500, Percent_Identity=22.4, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790505, Length=544, Percent_Identity=20.7720588235294, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786529, Length=526, Percent_Identity=21.6730038022814, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=24.1685144124169, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI32563687, Length=484, Percent_Identity=25.8264462809917, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=25.5020080321285, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=496, Percent_Identity=22.5806451612903, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=516, Percent_Identity=22.2868217054264, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=440, Percent_Identity=24.5454545454545, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=22.3282442748092, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=22.4568138195777, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=360, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994694, Length=360, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994690, Length=360, Percent_Identity=22.2222222222222, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=369, Percent_Identity=22.7642276422764, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=23.3201581027668, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24581924, Length=546, Percent_Identity=21.0622710622711, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24667959, Length=368, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62472339, Length=364, Percent_Identity=25, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667955, Length=384, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI281365686, Length=347, Percent_Identity=24.7838616714697, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356947, Length=364, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21355181, Length=484, Percent_Identity=22.1074380165289, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 54438; Mature: 54438

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIFDTGAPPACPPAFNLAAHVLRHAANRAGHPALVIAGPEQDQIWTYAAFERAVRGTA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHH
RGFLESGFEPGDILLMRLGNTVEFPITFLAAILVGIVPVPTSSQLTKREVARMVEDLSPA
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
GIVSAPDIATHPHSRAVSLDRLRAMQSCPPADAVLGDPDRLAYIVYTSGTGGSPRAVQHA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHH
HRAIWARQMMIRDWYDLSRNDRLLHAGAFNWTFTLGTGIMDPLSVGATAIIPSADTDLLD
HHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHHCCCEEEECCCCCCHHH
LPRVLTRTQATIFAAVPGVYRKILKCDTLPVLPDLRHGLAAGEALSGDIRDGWKDRIGTP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCHHHHCCCH
IFEAFGMSECSTFISSAPHRPSVAQSMGAPQRGRRIAIIGEDGPAPIGEEGIIAVHRSDP
HHHHHCHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCC
GLMLGYLNAPEETRARFDGEWFLTGDRGIMRADGQIIYCGRNDDMMNAGGFRVSPLEIET
CEEEEECCCCHHHHHCCCCCEEEECCCCEEEECCEEEEECCCCCCCCCCCEEECCEEEEE
VFATHPGLSQFAAAEVEVKPGVHIIVGVFKGREDVTEMALQAFAQERLAAYKRPKTYKRL
EEECCCCHHHHHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AEFPTNPNGKLLRRALPDLFKD
HHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSIFDTGAPPACPPAFNLAAHVLRHAANRAGHPALVIAGPEQDQIWTYAAFERAVRGTA
CCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEHHHHHHHHHHHHH
RGFLESGFEPGDILLMRLGNTVEFPITFLAAILVGIVPVPTSSQLTKREVARMVEDLSPA
HHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
GIVSAPDIATHPHSRAVSLDRLRAMQSCPPADAVLGDPDRLAYIVYTSGTGGSPRAVQHA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHH
HRAIWARQMMIRDWYDLSRNDRLLHAGAFNWTFTLGTGIMDPLSVGATAIIPSADTDLLD
HHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHHCCCEEEECCCCCCHHH
LPRVLTRTQATIFAAVPGVYRKILKCDTLPVLPDLRHGLAAGEALSGDIRDGWKDRIGTP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHHCHHHHCCCH
IFEAFGMSECSTFISSAPHRPSVAQSMGAPQRGRRIAIIGEDGPAPIGEEGIIAVHRSDP
HHHHHCHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEECCC
GLMLGYLNAPEETRARFDGEWFLTGDRGIMRADGQIIYCGRNDDMMNAGGFRVSPLEIET
CEEEEECCCCHHHHHCCCCCEEEECCCCEEEECCEEEEECCCCCCCCCCCEEECCEEEEE
VFATHPGLSQFAAAEVEVKPGVHIIVGVFKGREDVTEMALQAFAQERLAAYKRPKTYKRL
EEECCCCHHHHHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AEFPTNPNGKLLRRALPDLFKD
HHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]