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The map label for this gene is livK [C]

Identifier: 99081113

GI number: 99081113

Start: 1370689

End: 1371888

Strand: Direct

Name: livK [C]

Synonym: TM1040_1272

Alternate gene names: 99081113

Gene position: 1370689-1371888 (Clockwise)

Preceding gene: 99081111

Following gene: 99081114

Centisome position: 42.82

GC content: 60.5

Gene sequence:

>1200_bases
ATGAAAAAACTGCTGACCGCGACGGCAGCCATCGCATTGAGCGCTGGCACCGCGTTTGCAGACGGCCACGGCCACCCCGA
CGAAGTAAAGCTGGGTGTCCTGTTTGGCTTCACCGGCCCGATCGAATCCCTGGCGCCGACCATGGCCTCTGGTGCTGAAC
TCGCGATGTCCGAAGTCACCGAGTCCGGCAAACTCTTTGGCGGTGCAAAAGTGACCCCGATGCGCGCGGACACCGGCTGT
ATCGACAATGGTCTTGCGACTGCGAACGCAGAAAAGCTGATCGCAGATGGCGCCAACGGCATCGTGGGTGGTGACTGTTC
CGGCGTGACCGGCGCGATCCTGCAGAACGTCGCGATCCCGAACGGCATGGTGATGATTTCTCCCTCCGCAAGCTCGCCGG
GTCTGACCTCGATGGAAGACAACGGCCTGTTCTTCCGGACCACCCCGTCTGACGCACGTCAGGGCGAGATCATGGCGTCG
ATCCTTGCAGATCGTGGCGTCGACAGCATCGCCATCACCTATACCAACAACGATTACGGCAAGGGTCTGTCGGATTCGAT
CAAATCCGCATTCGAGGCCGCAGGCGGTGAAGTCACCATCGTGACCGCGCATGAAGACGGCAAGGGTGACTACTCTGCCG
AGGTTGCGGCGCTGGCATCAGCCGGTGGCGATATTCTGGTTGTTGCGGGCTATCTCGACCAGGGTGGTCTGGGCATCATC
CAGGGCGCGCTCGACACCGGTGCGTTCGACACCTTTGGTCTGCCGGACGGGATGATCGGCGATTCGCTGCCCAACAACGT
GGGCCCGGACCTCAATGGCTCCTTCGGGCAGATCGCCGGCTCTGACAGTGAAGGTGCCGAGATGTTCGCTGCCAAAGCCT
CCGAGCTTGGCTTTGACGGTACTTCTGCCTATTCGCCGGAATCCTATGATGCGGCAGCGCTTTTCCTGCTCGCGATGCAG
GCATCGGGCTCTGTTGATCCCAAGGATTACGTCGCCAAGATCACCGAAGTCGCCAATGCTCCGGGTGAGAAAATCAACCC
CGGTGAGCTCGGCAAAGCGCTCGAAATTCTCGCCAATGGCGGTGAGATCGACTATGAGGGCGCAACCGGCGTCAACCTGA
TCGGCCCCGGCGAGAGCGCAGGCTCTTTCCGTGAGATCGAAGTTCAGGACGGCAAGAACGTGACCGTGAAATTCCGCTAA

Upstream 100 bases:

>100_bases
TGTGACGTAACAAAAAGCCTGTCCGATCGCTCTTCCCTTTCTTGCGGGGCTTGCATAGGGTTCGTGTCGAACAACATTGT
TCCAATTAGGGAGAATTCAA

Downstream 100 bases:

>100_bases
GGCGGACCACTGTTGCACATCGCATCTGATCAGCCCGGGAGCCATTGCGCCCCGGGCTGTTCCAAATAAAAGGGCCGACA
AAGAGATCGGCAGGGGTAAA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKKLLTATAAIALSAGTAFADGHGHPDEVKLGVLFGFTGPIESLAPTMASGAELAMSEVTESGKLFGGAKVTPMRADTGC
IDNGLATANAEKLIADGANGIVGGDCSGVTGAILQNVAIPNGMVMISPSASSPGLTSMEDNGLFFRTTPSDARQGEIMAS
ILADRGVDSIAITYTNNDYGKGLSDSIKSAFEAAGGEVTIVTAHEDGKGDYSAEVAALASAGGDILVVAGYLDQGGLGII
QGALDTGAFDTFGLPDGMIGDSLPNNVGPDLNGSFGQIAGSDSEGAEMFAAKASELGFDGTSAYSPESYDAAALFLLAMQ
ASGSVDPKDYVAKITEVANAPGEKINPGELGKALEILANGGEIDYEGATGVNLIGPGESAGSFREIEVQDGKNVTVKFR

Sequences:

>Translated_399_residues
MKKLLTATAAIALSAGTAFADGHGHPDEVKLGVLFGFTGPIESLAPTMASGAELAMSEVTESGKLFGGAKVTPMRADTGC
IDNGLATANAEKLIADGANGIVGGDCSGVTGAILQNVAIPNGMVMISPSASSPGLTSMEDNGLFFRTTPSDARQGEIMAS
ILADRGVDSIAITYTNNDYGKGLSDSIKSAFEAAGGEVTIVTAHEDGKGDYSAEVAALASAGGDILVVAGYLDQGGLGII
QGALDTGAFDTFGLPDGMIGDSLPNNVGPDLNGSFGQIAGSDSEGAEMFAAKASELGFDGTSAYSPESYDAAALFLLAMQ
ASGSVDPKDYVAKITEVANAPGEKINPGELGKALEILANGGEIDYEGATGVNLIGPGESAGSFREIEVQDGKNVTVKFR
>Mature_399_residues
MKKLLTATAAIALSAGTAFADGHGHPDEVKLGVLFGFTGPIESLAPTMASGAELAMSEVTESGKLFGGAKVTPMRADTGC
IDNGLATANAEKLIADGANGIVGGDCSGVTGAILQNVAIPNGMVMISPSASSPGLTSMEDNGLFFRTTPSDARQGEIMAS
ILADRGVDSIAITYTNNDYGKGLSDSIKSAFEAAGGEVTIVTAHEDGKGDYSAEVAALASAGGDILVVAGYLDQGGLGII
QGALDTGAFDTFGLPDGMIGDSLPNNVGPDLNGSFGQIAGSDSEGAEMFAAKASELGFDGTSAYSPESYDAAALFLLAMQ
ASGSVDPKDYVAKITEVANAPGEKINPGELGKALEILANGGEIDYEGATGVNLIGPGESAGSFREIEVQDGKNVTVKFR

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=285, Percent_Identity=29.4736842105263, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994941, Length=273, Percent_Identity=28.9377289377289, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40125; Mature: 40125

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLTATAAIALSAGTAFADGHGHPDEVKLGVLFGFTGPIESLAPTMASGAELAMSEVT
CCHHHHHHHHHEEECCCEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
ESGKLFGGAKVTPMRADTGCIDNGLATANAEKLIADGANGIVGGDCSGVTGAILQNVAIP
HCCCEECCCEECEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
NGMVMISPSASSPGLTSMEDNGLFFRTTPSDARQGEIMASILADRGVDSIAITYTNNDYG
CCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
KGLSDSIKSAFEAAGGEVTIVTAHEDGKGDYSAEVAALASAGGDILVVAGYLDQGGLGII
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEE
QGALDTGAFDTFGLPDGMIGDSLPNNVGPDLNGSFGQIAGSDSEGAEMFAAKASELGFDG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
TSAYSPESYDAAALFLLAMQASGSVDPKDYVAKITEVANAPGEKINPGELGKALEILANG
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
GEIDYEGATGVNLIGPGESAGSFREIEVQDGKNVTVKFR
CEEEECCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MKKLLTATAAIALSAGTAFADGHGHPDEVKLGVLFGFTGPIESLAPTMASGAELAMSEVT
CCHHHHHHHHHEEECCCEECCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHHHHHH
ESGKLFGGAKVTPMRADTGCIDNGLATANAEKLIADGANGIVGGDCSGVTGAILQNVAIP
HCCCEECCCEECEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHCCC
NGMVMISPSASSPGLTSMEDNGLFFRTTPSDARQGEIMASILADRGVDSIAITYTNNDYG
CCEEEEECCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
KGLSDSIKSAFEAAGGEVTIVTAHEDGKGDYSAEVAALASAGGDILVVAGYLDQGGLGII
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCEEE
QGALDTGAFDTFGLPDGMIGDSLPNNVGPDLNGSFGQIAGSDSEGAEMFAAKASELGFDG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
TSAYSPESYDAAALFLLAMQASGSVDPKDYVAKITEVANAPGEKINPGELGKALEILANG
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
GEIDYEGATGVNLIGPGESAGSFREIEVQDGKNVTVKFR
CEEEECCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA