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The map label for this gene is gcvA [H]

Identifier: 99081097

GI number: 99081097

Start: 1353542

End: 1354474

Strand: Direct

Name: gcvA [H]

Synonym: TM1040_1256

Alternate gene names: 99081097

Gene position: 1353542-1354474 (Clockwise)

Preceding gene: 99081095

Following gene: 99081109

Centisome position: 42.29

GC content: 58.95

Gene sequence:

>933_bases
ATGTATGACAAAAAATCATACATGTCGGTACCTCGCAGATTTCTTCCTTCGATTGCGTCCCTCCGCGCGCTGGAGGCGGT
TGACCGCCTCGGGAGCGCTTCTGCGGCTGCGGTAGAGCTCTCCTTGACGCAAGGGGCTGTGAGTCGCCAGTTGCAGGCGC
TGGAGCGGCAGCTTGGCATTGAGCTTGTCCTGCGCGATGGAAAGGCGCTCTCTTTGACGCCAGATGCGACGGACTATGCC
CAGGAGGTACGCCATGCGCTCGACCGGATTGCGCAGGCAACCCTGCAGGTGCAATCCGTTCCATTGGCCGGAAGCCTGAA
CCTCGCCATTTTGCCCGCCTTTGGCATGCGCTGGCTGATGCCTCGTTTGCCGGCGTTCTCACGGCGACATCCGGATGTGA
CAATCAATATGTCCACCCGGTTGTCGCCTTTTAACTTTGCAACGCAAAGCTTTGATGCCGCACTTCATTTTGGACATGCA
GACTGGCCGGGGACGGATTCTCTGCTGCTCAAGCACGAGCAGCTTTTGCCGGTCTGTGCGCCGGAGCTTGTCAAACGCGC
CAAGGTTGATGCACCGGGCGATATCCTCGCCCTACCGCTTTTGCATATTCAAACCCGGCCGACCGCTTGGGCGGATTGGT
TTCAACGGCAGCAAGTTCCGACTTCGGGCACGCTCGGTGGTAGCATGTATGACCAGTTCTCGACCATCAATCAGGCTGCG
CTGCATGGTTTGGGGGTGGCCTTGATGCCGGATTATCTCGTGGAACAGGACCTCGCGACAGGGCGGCTGGTGTCATTGCA
TCCCGTGCCCGAAGACATTGGCGGCGCGTATTATCTGGTCTGGCCGACCAGCAAGTCTCGCCACAGCGGCCTTCAGAAAT
TTCGTGACTGGCTAGCCGAACAGGCACAGCCCGAAGATCCCTTGCCGCGCTAA

Upstream 100 bases:

>100_bases
CGAAGTGTGGGTTTGTCATCCAGCATGTGGGCCTCCAATCAAAGTGTTGACGAGAGCTTAAGCGCGGCTGGCCGCAAAAA
ACAGCGAGACTTTGGAAAGA

Downstream 100 bases:

>100_bases
GCCCACTGTTTGATGGAAAAACGGCTCCGGAAAACCGGAGCCGCAATAAATCGTTACAGTACAAATCTAATGTGGTCGGC
TACCCGAGCGCATAGCCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MYDKKSYMSVPRRFLPSIASLRALEAVDRLGSASAAAVELSLTQGAVSRQLQALERQLGIELVLRDGKALSLTPDATDYA
QEVRHALDRIAQATLQVQSVPLAGSLNLAILPAFGMRWLMPRLPAFSRRHPDVTINMSTRLSPFNFATQSFDAALHFGHA
DWPGTDSLLLKHEQLLPVCAPELVKRAKVDAPGDILALPLLHIQTRPTAWADWFQRQQVPTSGTLGGSMYDQFSTINQAA
LHGLGVALMPDYLVEQDLATGRLVSLHPVPEDIGGAYYLVWPTSKSRHSGLQKFRDWLAEQAQPEDPLPR

Sequences:

>Translated_310_residues
MYDKKSYMSVPRRFLPSIASLRALEAVDRLGSASAAAVELSLTQGAVSRQLQALERQLGIELVLRDGKALSLTPDATDYA
QEVRHALDRIAQATLQVQSVPLAGSLNLAILPAFGMRWLMPRLPAFSRRHPDVTINMSTRLSPFNFATQSFDAALHFGHA
DWPGTDSLLLKHEQLLPVCAPELVKRAKVDAPGDILALPLLHIQTRPTAWADWFQRQQVPTSGTLGGSMYDQFSTINQAA
LHGLGVALMPDYLVEQDLATGRLVSLHPVPEDIGGAYYLVWPTSKSRHSGLQKFRDWLAEQAQPEDPLPR
>Mature_310_residues
MYDKKSYMSVPRRFLPSIASLRALEAVDRLGSASAAAVELSLTQGAVSRQLQALERQLGIELVLRDGKALSLTPDATDYA
QEVRHALDRIAQATLQVQSVPLAGSLNLAILPAFGMRWLMPRLPAFSRRHPDVTINMSTRLSPFNFATQSFDAALHFGHA
DWPGTDSLLLKHEQLLPVCAPELVKRAKVDAPGDILALPLLHIQTRPTAWADWFQRQQVPTSGTLGGSMYDQFSTINQAA
LHGLGVALMPDYLVEQDLATGRLVSLHPVPEDIGGAYYLVWPTSKSRHSGLQKFRDWLAEQAQPEDPLPR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=30.6122448979592, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1786448, Length=283, Percent_Identity=29.6819787985866, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=28.8079470198675, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=25.752508361204, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=26.2237762237762, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=25.4416961130742, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1790262, Length=122, Percent_Identity=33.6065573770492, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=23.489932885906, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34171; Mature: 34171

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDKKSYMSVPRRFLPSIASLRALEAVDRLGSASAAAVELSLTQGAVSRQLQALERQLGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHCE
ELVLRDGKALSLTPDATDYAQEVRHALDRIAQATLQVQSVPLAGSLNLAILPAFGMRWLM
EEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHH
PRLPAFSRRHPDVTINMSTRLSPFNFATQSFDAALHFGHADWPGTDSLLLKHEQLLPVCA
HCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHH
PELVKRAKVDAPGDILALPLLHIQTRPTAWADWFQRQQVPTSGTLGGSMYDQFSTINQAA
HHHHHHHCCCCCCCEEEHHHHEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LHGLGVALMPDYLVEQDLATGRLVSLHPVPEDIGGAYYLVWPTSKSRHSGLQKFRDWLAE
HHHCCHHHHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHH
QAQPEDPLPR
CCCCCCCCCC
>Mature Secondary Structure
MYDKKSYMSVPRRFLPSIASLRALEAVDRLGSASAAAVELSLTQGAVSRQLQALERQLGI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEHHHHHHHHHHHHHHHHHCE
ELVLRDGKALSLTPDATDYAQEVRHALDRIAQATLQVQSVPLAGSLNLAILPAFGMRWLM
EEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHHHHH
PRLPAFSRRHPDVTINMSTRLSPFNFATQSFDAALHFGHADWPGTDSLLLKHEQLLPVCA
HCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHH
PELVKRAKVDAPGDILALPLLHIQTRPTAWADWFQRQQVPTSGTLGGSMYDQFSTINQAA
HHHHHHHCCCCCCCEEEHHHHEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LHGLGVALMPDYLVEQDLATGRLVSLHPVPEDIGGAYYLVWPTSKSRHSGLQKFRDWLAE
HHHCCHHHHHHHHHHHHCCCCCEEEECCCCHHHCCEEEEEECCCCHHHHHHHHHHHHHHH
QAQPEDPLPR
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]