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The map label for this gene is rsmB [H]

Identifier: 99081068

GI number: 99081068

Start: 1320632

End: 1321807

Strand: Direct

Name: rsmB [H]

Synonym: TM1040_1227

Alternate gene names: 99081068

Gene position: 1320632-1321807 (Clockwise)

Preceding gene: 99081067

Following gene: 99081069

Centisome position: 41.26

GC content: 61.39

Gene sequence:

>1176_bases
ATGACACCGGGTGCGCGCGTACAGGCGGCTATTGAAATTCTCGATGAAATCCTCGCGGGTCAGGCTGTGGAAAAGACCCT
GACCAACTGGGCGCGGCGCAGTCGATTTGCCGGATCAAAAGACCGCGCCGCGGTGCGCGATCACGTCTATCAGGCGCTTC
GGTGCCGCCGGTCCTATGCGGTTCTGGGGGGAAGTGAGACCGGACGGGGGCTGATGCTTGGCGCTTGCAAGGATCAAGGG
CTTGATCAGGCAGTGCTCTTTCACGGTGAAGGGCATGCGCCGTCCCCCCTCAGCGCCGCCGAACAGAGCATCGCGCCCGA
ATTTCACAGCGACGCAGAGCGTCATGATATCCCGGAGTGGCTCTGGCCCGTGTTTTCACGCAGCCTCGGCACTGAGGCGA
TTGCAGCGGCAACCGCGCTCAGGTCTCGAGCGGCAGTTCATCTGCGGGTGAACCTCTTGAAAGGAGATCGCGACAACGCC
ATCAAGCGGCTCACGCGGGAGGGCATTGCCACAGAGCCGCATCCAGCCTCGCCGACCGCCCTGACCGTGACAGAGGGGGC
GCGCCGCATAAAAAACGCAGAAAGCTACCTGCAAGGCTTTGTGGAATTGCAGGACGCTGCCAGTCAGGCAGTCGTCGATA
AGCTGCCAGTGCAAAATGCCCCGAGGATATTGGACTATTGTTCCGGCGGTGGAGGCAAGGCTCTGGCGATTGCAGCACAG
ACCCAGGCTGAGGTCTATGCGCATGATGCAGACCCACGACGCATGCGCGACATTCCCGAACGCGCCATGCGGGCGGGGGC
GGATATTCGCTGCCTTACCTCCGAGGAGCTTGTAACGCAGGCGCCGTTTGACCTCGTGCTCTGTGATGCGCCCTGCAGTG
GTAGCGGGTCTTGGAGGCGTGATCCCGAGGGTAAGTGGCGCCTCACGCAGGACACTCTTGATGACACCGTAGCGCTGCAG
GCCCGAATTCTGGATGAAGCTGCTCAACGCGTCGCGCCGGGGGGCGTCCTGGCCTTTGCGACCTGTTCGATGCTGGATGT
GGAAAACAGCCTGCAGACACAGCGCTTTCAGGAGCGGCACACCGGCTGGGCGCACTTGTCTGAAACGGCATGGCATGTGC
ATAGTGGAACAGACGGATTTTACGTATCGGTGTTTCGGCGGAATGGCACAGAATAA

Upstream 100 bases:

>100_bases
GTTGAAACCGCTTGTGGCGTGCCCGGCGCGGGCGTTAGAGAGGCATCCAAACGGAAACCGCGCCGGCAATCTGCATGGCG
TCTCTGGAGCGGATGCAAAT

Downstream 100 bases:

>100_bases
ACGATTGTAAAGTTTCGCATTTCGGCGCTGTGGTTAACCTCGCTTTAACCATCCGTGTCATGATAGTGCGTCAATAAGAC
TGTTAACGGAGCCGTAATGA

Product: Fmu (Sun)

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTPGARVQAAIEILDEILAGQAVEKTLTNWARRSRFAGSKDRAAVRDHVYQALRCRRSYAVLGGSETGRGLMLGACKDQG
LDQAVLFHGEGHAPSPLSAAEQSIAPEFHSDAERHDIPEWLWPVFSRSLGTEAIAAATALRSRAAVHLRVNLLKGDRDNA
IKRLTREGIATEPHPASPTALTVTEGARRIKNAESYLQGFVELQDAASQAVVDKLPVQNAPRILDYCSGGGGKALAIAAQ
TQAEVYAHDADPRRMRDIPERAMRAGADIRCLTSEELVTQAPFDLVLCDAPCSGSGSWRRDPEGKWRLTQDTLDDTVALQ
ARILDEAAQRVAPGGVLAFATCSMLDVENSLQTQRFQERHTGWAHLSETAWHVHSGTDGFYVSVFRRNGTE

Sequences:

>Translated_391_residues
MTPGARVQAAIEILDEILAGQAVEKTLTNWARRSRFAGSKDRAAVRDHVYQALRCRRSYAVLGGSETGRGLMLGACKDQG
LDQAVLFHGEGHAPSPLSAAEQSIAPEFHSDAERHDIPEWLWPVFSRSLGTEAIAAATALRSRAAVHLRVNLLKGDRDNA
IKRLTREGIATEPHPASPTALTVTEGARRIKNAESYLQGFVELQDAASQAVVDKLPVQNAPRILDYCSGGGGKALAIAAQ
TQAEVYAHDADPRRMRDIPERAMRAGADIRCLTSEELVTQAPFDLVLCDAPCSGSGSWRRDPEGKWRLTQDTLDDTVALQ
ARILDEAAQRVAPGGVLAFATCSMLDVENSLQTQRFQERHTGWAHLSETAWHVHSGTDGFYVSVFRRNGTE
>Mature_390_residues
TPGARVQAAIEILDEILAGQAVEKTLTNWARRSRFAGSKDRAAVRDHVYQALRCRRSYAVLGGSETGRGLMLGACKDQGL
DQAVLFHGEGHAPSPLSAAEQSIAPEFHSDAERHDIPEWLWPVFSRSLGTEAIAAATALRSRAAVHLRVNLLKGDRDNAI
KRLTREGIATEPHPASPTALTVTEGARRIKNAESYLQGFVELQDAASQAVVDKLPVQNAPRILDYCSGGGGKALAIAAQT
QAEVYAHDADPRRMRDIPERAMRAGADIRCLTSEELVTQAPFDLVLCDAPCSGSGSWRRDPEGKWRLTQDTLDDTVALQA
RILDEAAQRVAPGGVLAFATCSMLDVENSLQTQRFQERHTGWAHLSETAWHVHSGTDGFYVSVFRRNGTE

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=278, Percent_Identity=29.136690647482, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI76150623, Length=278, Percent_Identity=29.136690647482, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI32698918, Length=225, Percent_Identity=28.8888888888889, Blast_Score=69, Evalue=9e-12,
Organism=Escherichia coli, GI2367212, Length=298, Percent_Identity=29.5302013422819, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081985, Length=214, Percent_Identity=27.5700934579439, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17536757, Length=297, Percent_Identity=28.6195286195286, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=240, Percent_Identity=27.9166666666667, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI22024126, Length=276, Percent_Identity=27.1739130434783, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355201, Length=184, Percent_Identity=29.3478260869565, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 42593; Mature: 42462

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGARVQAAIEILDEILAGQAVEKTLTNWARRSRFAGSKDRAAVRDHVYQALRCRRSYA
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHE
VLGGSETGRGLMLGACKDQGLDQAVLFHGEGHAPSPLSAAEQSIAPEFHSDAERHDIPEW
EECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHCCCHHCCCHHH
LWPVFSRSLGTEAIAAATALRSRAAVHLRVNLLKGDRDNAIKRLTREGIATEPHPASPTA
HHHHHHHHCCHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCE
LTVTEGARRIKNAESYLQGFVELQDAASQAVVDKLPVQNAPRILDYCSGGGGKALAIAAQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEC
TQAEVYAHDADPRRMRDIPERAMRAGADIRCLTSEELVTQAPFDLVLCDAPCSGSGSWRR
CCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEECCCCCCCCCCCC
DPEGKWRLTQDTLDDTVALQARILDEAAQRVAPGGVLAFATCSMLDVENSLQTQRFQERH
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
TGWAHLSETAWHVHSGTDGFYVSVFRRNGTE
CCHHHHCCCEEEEECCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TPGARVQAAIEILDEILAGQAVEKTLTNWARRSRFAGSKDRAAVRDHVYQALRCRRSYA
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHE
VLGGSETGRGLMLGACKDQGLDQAVLFHGEGHAPSPLSAAEQSIAPEFHSDAERHDIPEW
EECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHCCCHHCCCHHH
LWPVFSRSLGTEAIAAATALRSRAAVHLRVNLLKGDRDNAIKRLTREGIATEPHPASPTA
HHHHHHHHCCHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCE
LTVTEGARRIKNAESYLQGFVELQDAASQAVVDKLPVQNAPRILDYCSGGGGKALAIAAQ
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEEEEC
TQAEVYAHDADPRRMRDIPERAMRAGADIRCLTSEELVTQAPFDLVLCDAPCSGSGSWRR
CCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHCCCCEEEECCCCCCCCCCCC
DPEGKWRLTQDTLDDTVALQARILDEAAQRVAPGGVLAFATCSMLDVENSLQTQRFQERH
CCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
TGWAHLSETAWHVHSGTDGFYVSVFRRNGTE
CCHHHHCCCEEEEECCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA