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The map label for this gene is proB

Identifier: 99081045

GI number: 99081045

Start: 1293829

End: 1294935

Strand: Direct

Name: proB

Synonym: TM1040_1204

Alternate gene names: 99081045

Gene position: 1293829-1294935 (Clockwise)

Preceding gene: 99081044

Following gene: 99081046

Centisome position: 40.42

GC content: 64.77

Gene sequence:

>1107_bases
TTGGCAGCCCTAAGCACGGCGCGGCGCGTCGTCATCAAGATCGGCTCCGCCCTTTTGGTGGATCGCACCAGCAGTGCCTT
GCGGCAGGAGTGGCTCCTGTCGCTGGCAGAGGATGTGGCCCGCCTGAAAAAACAGGGCAAGGATGTGATCCTCGTGTCCT
CGGGCTCGATTGCGCTGGGGCGCGGTGCCCTGGGCCTGCCGCGCAAGGATCTGCCGCTTGAACAGTCACAGGCCGCCGCA
GCTGTGGGCCAGATCCGGCTTGCGCGCGCTTATGAGGAGGCCCTGGCGCCGCATGGGATCACCACGGCGCAGGTGCTTGT
AACGCTGGAGGACAGCGAAAACCGCCGCCGTTACCTCAATTCCCGCGCCACGCTCGAGACTTTGATCGGTCTGGGCGCGG
TGCCCATCGTCAATGAGAACGATACCATCGCCACCGACGAGATCCGCTATGGCGACAACGACCGTCTGGCGGCCCAGGTC
GCGGTGACGGTGGGGGCCGATGTGCTGATCCTGTTGTCGGATGTGGACGGGTTCTACAGCGCCAATCCTGCGCTTGATCC
AGACGCCAAACGGTTTGACCGGATCGAACAGATCACGCCCGAGATCGAGGCAATGGCGGGCGATGGGGTCTCGGGGCTGT
CCAAAGGCGGCATGATCACCAAGCTCTTGGCGGCCAAGATGGCCACCGCCGCAGGCTGCGCCATGGCAATCTCCGAAGGA
TCGGTGATGTCGCCGGTTTCGGCACTTGAGGCTGGGGCTGCTTCGACGTGGTTCACAGCCCAAGGTGACCCGCAGGTCGC
GCGCAAACGCTGGATCGCCGCGATGAAAACCCGCGGCGTGATCACCGTGGATGAAGGTGCGGCCAAGGCGCTGCACAACG
GAAACAGTCTGCTGCCCGCAGGGGTGCGTCATGTGGAGGGTGACTTTGGCCGCGGAGATCCGCTGGCCATCCTCGGCCCA
GATGGCCGTAAGCTGGGGCAGGGGCTGAGCCGCTACACGGCCGAGGAAGCCCGCGCCCTTCAGGGACATCGTTCGGATGA
GATCGAGGCGATCCTCGGCTATGCTGGACGTGCGGCCCTGATCCACCGGGATGACATGGCGCTTTGA

Upstream 100 bases:

>100_bases
GGGAAGGTCTGGATCTGGTCCTGCGTGCGGTGCGCGCCGAGATCGATGATGACCGCCTTCGGATCAAACAGGCGGAGGCG
TCCGAGGAGGAGGACACCCC

Downstream 100 bases:

>100_bases
TCCTGTCATTCTGGCGCTAAGAAGACGTCCTGCCGGCGCGTGATGTGGCGGTATGGCTCGCACCCCGCCTGGGGAAGAAA
AGGGCAAAGACATGAAAGAT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MAALSTARRVVIKIGSALLVDRTSSALRQEWLLSLAEDVARLKKQGKDVILVSSGSIALGRGALGLPRKDLPLEQSQAAA
AVGQIRLARAYEEALAPHGITTAQVLVTLEDSENRRRYLNSRATLETLIGLGAVPIVNENDTIATDEIRYGDNDRLAAQV
AVTVGADVLILLSDVDGFYSANPALDPDAKRFDRIEQITPEIEAMAGDGVSGLSKGGMITKLLAAKMATAAGCAMAISEG
SVMSPVSALEAGAASTWFTAQGDPQVARKRWIAAMKTRGVITVDEGAAKALHNGNSLLPAGVRHVEGDFGRGDPLAILGP
DGRKLGQGLSRYTAEEARALQGHRSDEIEAILGYAGRAALIHRDDMAL

Sequences:

>Translated_368_residues
MAALSTARRVVIKIGSALLVDRTSSALRQEWLLSLAEDVARLKKQGKDVILVSSGSIALGRGALGLPRKDLPLEQSQAAA
AVGQIRLARAYEEALAPHGITTAQVLVTLEDSENRRRYLNSRATLETLIGLGAVPIVNENDTIATDEIRYGDNDRLAAQV
AVTVGADVLILLSDVDGFYSANPALDPDAKRFDRIEQITPEIEAMAGDGVSGLSKGGMITKLLAAKMATAAGCAMAISEG
SVMSPVSALEAGAASTWFTAQGDPQVARKRWIAAMKTRGVITVDEGAAKALHNGNSLLPAGVRHVEGDFGRGDPLAILGP
DGRKLGQGLSRYTAEEARALQGHRSDEIEAILGYAGRAALIHRDDMAL
>Mature_367_residues
AALSTARRVVIKIGSALLVDRTSSALRQEWLLSLAEDVARLKKQGKDVILVSSGSIALGRGALGLPRKDLPLEQSQAAAA
VGQIRLARAYEEALAPHGITTAQVLVTLEDSENRRRYLNSRATLETLIGLGAVPIVNENDTIATDEIRYGDNDRLAAQVA
VTVGADVLILLSDVDGFYSANPALDPDAKRFDRIEQITPEIEAMAGDGVSGLSKGGMITKLLAAKMATAAGCAMAISEGS
VMSPVSALEAGAASTWFTAQGDPQVARKRWIAAMKTRGVITVDEGAAKALHNGNSLLPAGVRHVEGDFGRGDPLAILGPD
GRKLGQGLSRYTAEEARALQGHRSDEIEAILGYAGRAALIHRDDMAL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=270, Percent_Identity=31.4814814814815, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI62912457, Length=268, Percent_Identity=31.7164179104478, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=41.9178082191781, Blast_Score=267, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17569967, Length=310, Percent_Identity=32.258064516129, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17569969, Length=310, Percent_Identity=32.258064516129, Blast_Score=125, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=32.1428571428571, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=31.6666666666667, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21357643, Length=288, Percent_Identity=32.9861111111111, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SILST (Q1GHC9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613199.1
- ProteinModelPortal:   Q1GHC9
- SMR:   Q1GHC9
- STRING:   Q1GHC9
- GeneID:   4076339
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1204
- NMPDR:   fig|292414.1.peg.3664
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q1GHC9
- ProtClustDB:   PRK05429
- BioCyc:   SSP292414:TM1040_1204-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38733; Mature: 38601

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALSTARRVVIKIGSALLVDRTSSALRQEWLLSLAEDVARLKKQGKDVILVSSGSIALG
CCCHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEC
RGALGLPRKDLPLEQSQAAAAVGQIRLARAYEEALAPHGITTAQVLVTLEDSENRRRYLN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHH
SRATLETLIGLGAVPIVNENDTIATDEIRYGDNDRLAAQVAVTVGADVLILLSDVDGFYS
HHHHHHHHHCCCCCEEECCCCCEECCCEEECCCCCEEEEEEEEECCCEEEEEECCCCCCC
ANPALDPDAKRFDRIEQITPEIEAMAGDGVSGLSKGGMITKLLAAKMATAAGCAMAISEG
CCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCC
SVMSPVSALEAGAASTWFTAQGDPQVARKRWIAAMKTRGVITVDEGAAKALHNGNSLLPA
CCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCC
GVRHVEGDFGRGDPLAILGPDGRKLGQGLSRYTAEEARALQGHRSDEIEAILGYAGRAAL
CHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
IHRDDMAL
EECCCCCC
>Mature Secondary Structure 
AALSTARRVVIKIGSALLVDRTSSALRQEWLLSLAEDVARLKKQGKDVILVSSGSIALG
CCHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEC
RGALGLPRKDLPLEQSQAAAAVGQIRLARAYEEALAPHGITTAQVLVTLEDSENRRRYLN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHHHHH
SRATLETLIGLGAVPIVNENDTIATDEIRYGDNDRLAAQVAVTVGADVLILLSDVDGFYS
HHHHHHHHHCCCCCEEECCCCCEECCCEEECCCCCEEEEEEEEECCCEEEEEECCCCCCC
ANPALDPDAKRFDRIEQITPEIEAMAGDGVSGLSKGGMITKLLAAKMATAAGCAMAISEG
CCCCCCCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCC
SVMSPVSALEAGAASTWFTAQGDPQVARKRWIAAMKTRGVITVDEGAAKALHNGNSLLPA
CCCCHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCC
GVRHVEGDFGRGDPLAILGPDGRKLGQGLSRYTAEEARALQGHRSDEIEAILGYAGRAAL
CHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEE
IHRDDMAL
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA