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The map label for this gene is rlmE

Identifier: 99081023

GI number: 99081023

Start: 1271718

End: 1272434

Strand: Reverse

Name: rlmE

Synonym: TM1040_1182

Alternate gene names: 99081023

Gene position: 1272434-1271718 (Counterclockwise)

Preceding gene: 99081024

Following gene: 99081019

Centisome position: 39.75

GC content: 59.83

Gene sequence:

>717_bases
ATGGCGAAGAAACCAACAGGAAAGAACACCTCTGGCCGGGGCCAGCGGGATCTGACCGTCAAGGTCAAGACCGCGCGTGG
GCGCCGACTGAGCTCGACCCGCTGGCTGCAGCGGCAGCTCAATGATCCATATGTCAAACGGGCGCAGGCCGAGGGCTATC
GCGGACGCGCGGCGTTCAAGATCATGGAACTCGACGACAAATACCGCTTTCTTGTGCCCGGGGCCCGGGTTGTTGATCTG
GGCTGTGCGCCGGGCGGTTGGGCACAGGTGGCGGTGCCGCGCATCAATGCGCTGGGCGAGAAATCCGGCAAAGCCATTGG
ACGTTTCATCGGCATTGACCTGCAAGAGGTCGAACCACTTGCAGGCGCCGAGTTCCATCAGCTCGACTTTATGGACGAAG
GCGCGGACGATCAGGTCAAGGAATGGTTAGGCGGTCAGGCGGATGTGGTGATGTCGGACATGGCGGCCTCCAGTTCGGGG
CACAAGCAGACGGACCACCTGCGGATTATGTCTCTCTGCGAGACCGCTGCCTATTTCGCCTTTGATGTACTCGAGGAAGG
CGGCACCTTTGTCGCCAAGGTTCTGGCCGGTGGTGCCGAAGGCGAACTGCAAAAGCTGCTGAAGCAGAAATTCAAATCCG
TCGCCAATGTAAAGCCGCCCTCCTCACGGGCTGACAGTTCGGAGAAATTTGTTGTGGCGACAGGCTTTAGAGGCTGA

Upstream 100 bases:

>100_bases
GGACGGCCCTTTCTGAGGCCCCTTTCTGCGCCGGCAAAACTCGGTTATAGCCCCAAAGCCGCGCCTTTGAGGTGGCGCAC
GACGTGTAAGAGGAATGACC

Downstream 100 bases:

>100_bases
TCTTCACCCGCCGAACATCAAAAGCGGTGTCTAACCTCTGTCGCGCATCGCTTTGGGGCGCAGCGACTTTACCGCGAGAT
CTCTGAGCGACAGGAGCGGA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MAKKPTGKNTSGRGQRDLTVKVKTARGRRLSSTRWLQRQLNDPYVKRAQAEGYRGRAAFKIMELDDKYRFLVPGARVVDL
GCAPGGWAQVAVPRINALGEKSGKAIGRFIGIDLQEVEPLAGAEFHQLDFMDEGADDQVKEWLGGQADVVMSDMAASSSG
HKQTDHLRIMSLCETAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQKFKSVANVKPPSSRADSSEKFVVATGFRG

Sequences:

>Translated_238_residues
MAKKPTGKNTSGRGQRDLTVKVKTARGRRLSSTRWLQRQLNDPYVKRAQAEGYRGRAAFKIMELDDKYRFLVPGARVVDL
GCAPGGWAQVAVPRINALGEKSGKAIGRFIGIDLQEVEPLAGAEFHQLDFMDEGADDQVKEWLGGQADVVMSDMAASSSG
HKQTDHLRIMSLCETAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQKFKSVANVKPPSSRADSSEKFVVATGFRG
>Mature_237_residues
AKKPTGKNTSGRGQRDLTVKVKTARGRRLSSTRWLQRQLNDPYVKRAQAEGYRGRAAFKIMELDDKYRFLVPGARVVDLG
CAPGGWAQVAVPRINALGEKSGKAIGRFIGIDLQEVEPLAGAEFHQLDFMDEGADDQVKEWLGGQADVVMSDMAASSSGH
KQTDHLRIMSLCETAAYFAFDVLEEGGTFVAKVLAGGAEGELQKLLKQKFKSVANVKPPSSRADSSEKFVVATGFRG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=206, Percent_Identity=42.7184466019417, Blast_Score=181, Evalue=5e-46,
Organism=Homo sapiens, GI29029591, Length=196, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI29029589, Length=196, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI7110661, Length=196, Percent_Identity=34.6938775510204, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI194097365, Length=196, Percent_Identity=32.6530612244898, Blast_Score=96, Evalue=2e-20,
Organism=Escherichia coli, GI1789569, Length=213, Percent_Identity=43.1924882629108, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17553474, Length=211, Percent_Identity=35.5450236966825, Blast_Score=144, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17554650, Length=200, Percent_Identity=34, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=204, Percent_Identity=32.843137254902, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=204, Percent_Identity=32.843137254902, Blast_Score=94, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71987550, Length=204, Percent_Identity=32.843137254902, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=33.6683417085427, Blast_Score=106, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319796, Length=201, Percent_Identity=31.8407960199005, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321302, Length=137, Percent_Identity=33.5766423357664, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=38.9908256880734, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24648639, Length=196, Percent_Identity=34.1836734693878, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=35.8585858585859, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI18859957, Length=196, Percent_Identity=34.1836734693878, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_SILST (Q1GHF1)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613177.1
- ProteinModelPortal:   Q1GHF1
- SMR:   Q1GHF1
- STRING:   Q1GHF1
- GeneID:   4077791
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1182
- NMPDR:   fig|292414.1.peg.3685
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q1GHF1
- ProtClustDB:   CLSK933653
- BioCyc:   SSP292414:TM1040_1182-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 25893; Mature: 25762

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: ACT_SITE 193-193 BINDING 85-85 BINDING 87-87 BINDING 113-113 BINDING 129-129 BINDING 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPTGKNTSGRGQRDLTVKVKTARGRRLSSTRWLQRQLNDPYVKRAQAEGYRGRAAFK
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEE
IMELDDKYRFLVPGARVVDLGCAPGGWAQVAVPRINALGEKSGKAIGRFIGIDLQEVEPL
EEEECCCEEEEECCCEEEEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHCCCHHHCCCC
AGAEFHQLDFMDEGADDQVKEWLGGQADVVMSDMAASSSGHKQTDHLRIMSLCETAAYFA
CCCCCCEECCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FDVLEEGGTFVAKVLAGGAEGELQKLLKQKFKSVANVKPPSSRADSSEKFVVATGFRG
HHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
AKKPTGKNTSGRGQRDLTVKVKTARGRRLSSTRWLQRQLNDPYVKRAQAEGYRGRAAFK
CCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCEEEE
IMELDDKYRFLVPGARVVDLGCAPGGWAQVAVPRINALGEKSGKAIGRFIGIDLQEVEPL
EEEECCCEEEEECCCEEEEECCCCCCCEEEECHHHHHHCCCCHHHHHHHHCCCHHHCCCC
AGAEFHQLDFMDEGADDQVKEWLGGQADVVMSDMAASSSGHKQTDHLRIMSLCETAAYFA
CCCCCCEECCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
FDVLEEGGTFVAKVLAGGAEGELQKLLKQKFKSVANVKPPSSRADSSEKFVVATGFRG
HHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA