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The map label for this gene is paaF [C]

Identifier: 99081012

GI number: 99081012

Start: 1261221

End: 1262021

Strand: Reverse

Name: paaF [C]

Synonym: TM1040_1171

Alternate gene names: 99081012

Gene position: 1262021-1261221 (Counterclockwise)

Preceding gene: 99081013

Following gene: 99081011

Centisome position: 39.43

GC content: 62.67

Gene sequence:

>801_bases
ATGCAAACGGATGCAATCCTCCTCAAGCAATTGAGCGAGACCGGCGTGCTGCGCCTCACGCTGAATGATGTCGCGCGCCG
CAATGCACTCTCCGAGGCGATGCTGGGCACGCTCGGCGAGGCCTTAGCCAGCGCGGGTCAGGACCCCGCGGTACGTGTGG
TGGTGCTTGCGGCAAATGGCCCTGCGTTCAGCGCAGGTCATGACCTAAAGGAGCTGACCCGCGGCCGCGCGGCGCCGGAT
GGCGGCAAGGCCTATTTTGCGCGGATCATGGCGCTTTGCTCGGCCGTCATGCAGAGCATTGTGACCTGCCCCAAACCCGT
CATCGCCGAAGTCACCGGCGTCGCGACCGCTGCGGGCTGTCAGCTTGTGGCCAGCTGCGACCTCGCGATCGCCGCCGAGA
GCGCACGCTTCAGCACGCCGGGTGTTCACATCGGGCTGTTTTGCTCCACCCCGATGGTTGCCTTGTCCCGCAATGTGGCT
GGCAAACACGCCATGGAAATGCTGCTGACGGGCGACATGACCTCTGCGGAGCGCGCCGCCGAGATCGGTCTTGTCAACCG
CGTCACCTCCGAAGACGGCTTGCAGGACGCGGTGCTGGAACTGGCGCATAAGATCGCGTCCAAATCCTCCATGACGCTCG
CCACTGGCAAGCAGGCGTTTTATGCGCAGCGCGAAATGCCCCTGTCGGACGCCTATGACTATGCAGCGGACGTGATGGTC
AAAAACATGTTGGCGCAGGATGCCGCAGAAGGCATCAATGCCTTTGTCGAAAAACGTTCCCCGCAATGGCAGGATGCATA
A

Upstream 100 bases:

>100_bases
GAGGAGCTCGCCCAGATGCAGGACCGCGCGCACCGCTACTGCTTTATCGCCAATACCCTGGCCGATAGCGTTGAAATCAA
CATTCATTGAAAGAACACCC

Downstream 100 bases:

>100_bases
GCCGATGAGCGCCAACCCCTACAATACCGATCTGGATCGCACCCCGGCCAACTATCAGCCCCTGACCCCGCTCATGTTTC
TGGAGCGGGCCGCAACGGTA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MQTDAILLKQLSETGVLRLTLNDVARRNALSEAMLGTLGEALASAGQDPAVRVVVLAANGPAFSAGHDLKELTRGRAAPD
GGKAYFARIMALCSAVMQSIVTCPKPVIAEVTGVATAAGCQLVASCDLAIAAESARFSTPGVHIGLFCSTPMVALSRNVA
GKHAMEMLLTGDMTSAERAAEIGLVNRVTSEDGLQDAVLELAHKIASKSSMTLATGKQAFYAQREMPLSDAYDYAADVMV
KNMLAQDAAEGINAFVEKRSPQWQDA

Sequences:

>Translated_266_residues
MQTDAILLKQLSETGVLRLTLNDVARRNALSEAMLGTLGEALASAGQDPAVRVVVLAANGPAFSAGHDLKELTRGRAAPD
GGKAYFARIMALCSAVMQSIVTCPKPVIAEVTGVATAAGCQLVASCDLAIAAESARFSTPGVHIGLFCSTPMVALSRNVA
GKHAMEMLLTGDMTSAERAAEIGLVNRVTSEDGLQDAVLELAHKIASKSSMTLATGKQAFYAQREMPLSDAYDYAADVMV
KNMLAQDAAEGINAFVEKRSPQWQDA
>Mature_266_residues
MQTDAILLKQLSETGVLRLTLNDVARRNALSEAMLGTLGEALASAGQDPAVRVVVLAANGPAFSAGHDLKELTRGRAAPD
GGKAYFARIMALCSAVMQSIVTCPKPVIAEVTGVATAAGCQLVASCDLAIAAESARFSTPGVHIGLFCSTPMVALSRNVA
GKHAMEMLLTGDMTSAERAAEIGLVNRVTSEDGLQDAVLELAHKIASKSSMTLATGKQAFYAQREMPLSDAYDYAADVMV
KNMLAQDAAEGINAFVEKRSPQWQDA

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=45.7831325301205, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=33.8645418326693, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI70995211, Length=252, Percent_Identity=28.1746031746032, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=26.171875, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI45643119, Length=256, Percent_Identity=26.171875, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=256, Percent_Identity=26.171875, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502327, Length=273, Percent_Identity=25.2747252747253, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI221136756, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221136753, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI221307494, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=33.9920948616601, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI221142681, Length=263, Percent_Identity=32.319391634981, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=30.5019305019305, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=33.0708661417323, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1788597, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=26.8041237113402, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=249, Percent_Identity=43.7751004016064, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=30.7692307692308, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=31.4176245210728, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=239, Percent_Identity=26.3598326359833, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=24.1379310344828, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=264, Percent_Identity=26.1363636363636, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=25.7918552036199, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922422, Length=262, Percent_Identity=36.2595419847328, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=29.4820717131474, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=29.4820717131474, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24653139, Length=267, Percent_Identity=27.7153558052434, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=26.5873015873016, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654903, Length=202, Percent_Identity=26.7326732673267, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=217, Percent_Identity=26.2672811059908, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27856; Mature: 27856

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTDAILLKQLSETGVLRLTLNDVARRNALSEAMLGTLGEALASAGQDPAVRVVVLAANG
CCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
PAFSAGHDLKELTRGRAAPDGGKAYFARIMALCSAVMQSIVTCPKPVIAEVTGVATAAGC
CCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QLVASCDLAIAAESARFSTPGVHIGLFCSTPMVALSRNVAGKHAMEMLLTGDMTSAERAA
HHHHHCCHHEEECCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
EIGLVNRVTSEDGLQDAVLELAHKIASKSSMTLATGKQAFYAQREMPLSDAYDYAADVMV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHH
KNMLAQDAAEGINAFVEKRSPQWQDA
HHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQTDAILLKQLSETGVLRLTLNDVARRNALSEAMLGTLGEALASAGQDPAVRVVVLAANG
CCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
PAFSAGHDLKELTRGRAAPDGGKAYFARIMALCSAVMQSIVTCPKPVIAEVTGVATAAGC
CCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
QLVASCDLAIAAESARFSTPGVHIGLFCSTPMVALSRNVAGKHAMEMLLTGDMTSAERAA
HHHHHCCHHEEECCCCCCCCCEEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHH
EIGLVNRVTSEDGLQDAVLELAHKIASKSSMTLATGKQAFYAQREMPLSDAYDYAADVMV
HHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHCCCHHHHHHHHHHHHH
KNMLAQDAAEGINAFVEKRSPQWQDA
HHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA