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The map label for this gene is mmsB [H]

Identifier: 99080942

GI number: 99080942

Start: 1181900

End: 1182772

Strand: Reverse

Name: mmsB [H]

Synonym: TM1040_1101

Alternate gene names: 99080942

Gene position: 1182772-1181900 (Counterclockwise)

Preceding gene: 99080943

Following gene: 99080940

Centisome position: 36.95

GC content: 64.26

Gene sequence:

>873_bases
ATGCAAATCGGATTTATCGGACTGGGCAACATGGGCGGCCCGATGGCGGCCAATCTCGCCAAGGCAGGCCATAGCGTTAC
CGGCTTTGACATGGCGGATGTCTCGATCGAAGGCGTCTCCCTCGCCAAGAGCGCGGCCGAGGCCGCAACGGGCGCGGATG
TGGTGATCACCATGCTGCCCAATGGTGACATCCTGCGCCATGTCGCCTCCGAGATCATCCCCGCCATGACCAATGGCGCG
GCTTTTGTGGATTGCTCCACCGTTGATGTGGACAGCGCCCGCGCGGTGGCGCAACAGGCGGCAGAGGCCGGGCTTCTGGC
CGTGGATGCGCCGGTCTCCGGTGGCATCGGTGGGGCTGCGGGCGGCACGCTCACCTTTATGGCGGGCGGATCTGAGGAGG
CCTTTGCCAAGGCCGCACCGCTCTTTGATATCATGGGCCAGAAGGCCGTGCATTGCGGCGACTCCGGCGCCGGTCAGGCC
GCCAAGATCTGCAACAACATGATCCTCGGCGTCACCATGATCGCCACCTGCGAAGCCTTTGCGCTGGCCGACAAACTCGG
CCTTGATCGCCAGAAGATGTTCGATGTGGTCTCGACCTCCTCAGGCTACAGCTGGACCATGAACGCCTATTGCCCCGCAC
CCGGCGTCGGCCCGCAGTCCCCCGCCGACAACGATTACCAGCCGGGCTTTGCCGCCGATTTGATGCTGAAGGACCTGCGC
CTGTCGCAGCAGGCCGCCGGCAGCGCCGATGCCGACACCCCGATGGGCAAACTGGCCGAAGCGCTCTACAGCCAGTTCGT
CGACAAAGAGGACGGCAAGGGCAAGGATTTCTCCGCCATGTTGCCGCGCTTTGAAAAACGCGGCCGCAGCTAG

Upstream 100 bases:

>100_bases
AAGTGGCAATATGCCGACGGCAACGTGCCTGCGGTCGCCGTGAGCCAGATGCTCCAACCGCTTGGAGCCGAGGCATTGAG
TTTTGAGGAGGAGAGCTAAG

Downstream 100 bases:

>100_bases
GACGCCCCCATCTGGATGAGCGCCACTCGCGTGGCCTCATCCGCCGGGAACATGCATTCGATCCTGAGCTCGGAGAGGGC
AATATCCTCCACCGTGCCAA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MQIGFIGLGNMGGPMAANLAKAGHSVTGFDMADVSIEGVSLAKSAAEAATGADVVITMLPNGDILRHVASEIIPAMTNGA
AFVDCSTVDVDSARAVAQQAAEAGLLAVDAPVSGGIGGAAGGTLTFMAGGSEEAFAKAAPLFDIMGQKAVHCGDSGAGQA
AKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNDYQPGFAADLMLKDLR
LSQQAAGSADADTPMGKLAEALYSQFVDKEDGKGKDFSAMLPRFEKRGRS

Sequences:

>Translated_290_residues
MQIGFIGLGNMGGPMAANLAKAGHSVTGFDMADVSIEGVSLAKSAAEAATGADVVITMLPNGDILRHVASEIIPAMTNGA
AFVDCSTVDVDSARAVAQQAAEAGLLAVDAPVSGGIGGAAGGTLTFMAGGSEEAFAKAAPLFDIMGQKAVHCGDSGAGQA
AKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNDYQPGFAADLMLKDLR
LSQQAAGSADADTPMGKLAEALYSQFVDKEDGKGKDFSAMLPRFEKRGRS
>Mature_290_residues
MQIGFIGLGNMGGPMAANLAKAGHSVTGFDMADVSIEGVSLAKSAAEAATGADVVITMLPNGDILRHVASEIIPAMTNGA
AFVDCSTVDVDSARAVAQQAAEAGLLAVDAPVSGGIGGAAGGTLTFMAGGSEEAFAKAAPLFDIMGQKAVHCGDSGAGQA
AKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNDYQPGFAADLMLKDLR
LSQQAAGSADADTPMGKLAEALYSQFVDKEDGKGKDFSAMLPRFEKRGRS

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=287, Percent_Identity=42.5087108013937, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI40556376, Length=272, Percent_Identity=27.2058823529412, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI145693186, Length=298, Percent_Identity=32.8859060402685, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1786719, Length=271, Percent_Identity=30.9963099630996, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=31.6326530612245, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789092, Length=294, Percent_Identity=29.2517006802721, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17557316, Length=293, Percent_Identity=38.5665529010239, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=39.7260273972603, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=39.7260273972603, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI28574115, Length=272, Percent_Identity=23.1617647058824, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29500; Mature: 29500

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGFIGLGNMGGPMAANLAKAGHSVTGFDMADVSIEGVSLAKSAAEAATGADVVITMLP
CEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEECC
NGDILRHVASEIIPAMTNGAAFVDCSTVDVDSARAVAQQAAEAGLLAVDAPVSGGIGGAA
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
GGTLTFMAGGSEEAFAKAAPLFDIMGQKAVHCGDSGAGQAAKICNNMILGVTMIATCEAF
CCEEEEEECCCHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNDYQPGFAADLMLKDLR
HHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LSQQAAGSADADTPMGKLAEALYSQFVDKEDGKGKDFSAMLPRFEKRGRS
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQIGFIGLGNMGGPMAANLAKAGHSVTGFDMADVSIEGVSLAKSAAEAATGADVVITMLP
CEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEEECC
NGDILRHVASEIIPAMTNGAAFVDCSTVDVDSARAVAQQAAEAGLLAVDAPVSGGIGGAA
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCC
GGTLTFMAGGSEEAFAKAAPLFDIMGQKAVHCGDSGAGQAAKICNNMILGVTMIATCEAF
CCEEEEEECCCHHHHHHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNDYQPGFAADLMLKDLR
HHHHHHCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LSQQAAGSADADTPMGKLAEALYSQFVDKEDGKGKDFSAMLPRFEKRGRS
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]