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The map label for this gene is pgk

Identifier: 99080925

GI number: 99080925

Start: 1159751

End: 1160941

Strand: Reverse

Name: pgk

Synonym: TM1040_1084

Alternate gene names: 99080925

Gene position: 1160941-1159751 (Counterclockwise)

Preceding gene: 99080928

Following gene: 99080924

Centisome position: 36.27

GC content: 63.64

Gene sequence:

>1191_bases
ATGGGCTGGAAGACACTCGACGACATGGATCTGCAAGGCAAAAGCGTTTTGACCCGCGTGGATATCAACGTCCCGATGGA
AGACGGCAAAGTCACCGATGACACCCGCATCCGCCGCCTCGTGCCGACGATTTCCGACCTGCTCTCCAAGGGTGCAAAAC
CTGTGCTGCTGGCGCATTTTGGCCGCCCGAAGGGCAAGGTGGTGCCCGAGATGTCGTTGCAGCCGCTGGTCCCCGCGCTG
GAGGCCGCCTTTGGCGCGCCGGTGATGTTTGTGGCCGATTGCCGAGGCCCCGCTGCAAAAGGCGCCGTTGACGCGCTCGC
GCCCGGGCAGATCCTGCTCCTTGAGAACACGCGCTTTTATCCCGGCGAAGAACAAAACGATGCCGATCTCGCCAAAGAGA
TGGCCGCGCTTGGTGATATCTACTGCAATGATGCCTTCTCTGCCGCCCACCGCGCCCATGCCTCCACCGAGGCGCTGGCG
CGCCTGCTGCCTGCCTGCGCGGGCCGCCTGATGGAGGCAGAACTGAAAGCGCTCGAAGGCTCTCTGGCCACGCCTGAACG
CCCCGTCACTGCGGTTGTGGGCGGCGCCAAAGTTTCGACCAAACTGGACCTTCTGGGCAATCTGGTGGCCAAGGTAGACA
CGCTTGTGATCGGCGGCGGCATGGCCAATACCTTTCTTGCGGCCCAAGGCATCGACGTGGGCAAATCGCTTTGCGAACAT
GACATGACAGGCACCGCCAAGGACATCATGGCCCGCGCCGAGGCGGCAGGCTGCACCATCCTGCTGCCGTCGGACGTGGT
GGTCGCGCGCGAATTCAAGGCCGGCGCCGACAATGAAACCGTACCCGCGGACGCCTGCCCTGCGGATGCGATGATCCTGG
ATGCTGGCCCCGCCTCGGTTGCGGCCATTTCCACAGTCTTTGCCGAGAGCAAGACCCTGATCTGGAACGGCCCGCTTGGT
GCCTTTGAAATCGCGCCCTTTGATATTGCGACCAATACAGCGGCGCAAAAGGCGGCAGAACTCACCAAGGCCGGCCAGCT
GATTTCCGTTGCGGGCGGAGGCGACACCGTCGCTGCGCTCAATCAGGCCGGAGCGGCAGAGGCCTTTAGCTACATCTCCA
CCGCCGGGGGCGCCTTCCTTGAGTGGATGGAGGGCAAGGTCCTGCCCGGCGTCGCTGCCCTGCAGACCTGA

Upstream 100 bases:

>100_bases
CCCATTGACTGACGCCCGGAAACGCAGGACACAGGCGCAGGACATTGAATTTTCTGCGCCTGCACTCCGCGGGCGCGTCG
CACTGACAGAGGAGCCGGAC

Downstream 100 bases:

>100_bases
ACCTGCGCCCGGCCCCGCCCTGCCGCAAGGGCTGGGGCCAACTGACGCGCCCTGCCCCCGGCCAGCTCGGTCAATTCGGC
CAGCCCGGCCAGACCTGTCG

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGWKTLDDMDLQGKSVLTRVDINVPMEDGKVTDDTRIRRLVPTISDLLSKGAKPVLLAHFGRPKGKVVPEMSLQPLVPAL
EAAFGAPVMFVADCRGPAAKGAVDALAPGQILLLENTRFYPGEEQNDADLAKEMAALGDIYCNDAFSAAHRAHASTEALA
RLLPACAGRLMEAELKALEGSLATPERPVTAVVGGAKVSTKLDLLGNLVAKVDTLVIGGGMANTFLAAQGIDVGKSLCEH
DMTGTAKDIMARAEAAGCTILLPSDVVVAREFKAGADNETVPADACPADAMILDAGPASVAAISTVFAESKTLIWNGPLG
AFEIAPFDIATNTAAQKAAELTKAGQLISVAGGGDTVAALNQAGAAEAFSYISTAGGAFLEWMEGKVLPGVAALQT

Sequences:

>Translated_396_residues
MGWKTLDDMDLQGKSVLTRVDINVPMEDGKVTDDTRIRRLVPTISDLLSKGAKPVLLAHFGRPKGKVVPEMSLQPLVPAL
EAAFGAPVMFVADCRGPAAKGAVDALAPGQILLLENTRFYPGEEQNDADLAKEMAALGDIYCNDAFSAAHRAHASTEALA
RLLPACAGRLMEAELKALEGSLATPERPVTAVVGGAKVSTKLDLLGNLVAKVDTLVIGGGMANTFLAAQGIDVGKSLCEH
DMTGTAKDIMARAEAAGCTILLPSDVVVAREFKAGADNETVPADACPADAMILDAGPASVAAISTVFAESKTLIWNGPLG
AFEIAPFDIATNTAAQKAAELTKAGQLISVAGGGDTVAALNQAGAAEAFSYISTAGGAFLEWMEGKVLPGVAALQT
>Mature_395_residues
GWKTLDDMDLQGKSVLTRVDINVPMEDGKVTDDTRIRRLVPTISDLLSKGAKPVLLAHFGRPKGKVVPEMSLQPLVPALE
AAFGAPVMFVADCRGPAAKGAVDALAPGQILLLENTRFYPGEEQNDADLAKEMAALGDIYCNDAFSAAHRAHASTEALAR
LLPACAGRLMEAELKALEGSLATPERPVTAVVGGAKVSTKLDLLGNLVAKVDTLVIGGGMANTFLAAQGIDVGKSLCEHD
MTGTAKDIMARAEAAGCTILLPSDVVVAREFKAGADNETVPADACPADAMILDAGPASVAAISTVFAESKTLIWNGPLGA
FEIAPFDIATNTAAQKAAELTKAGQLISVAGGGDTVAALNQAGAAEAFSYISTAGGAFLEWMEGKVLPGVAALQT

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=39.4160583941606, Blast_Score=283, Evalue=1e-76,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=39.0776699029126, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1789294, Length=402, Percent_Identity=46.2686567164179, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17508823, Length=407, Percent_Identity=40.7862407862408, Blast_Score=291, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=407, Percent_Identity=38.3292383292383, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24581203, Length=409, Percent_Identity=40.5867970660147, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=38.4428223844282, Blast_Score=253, Evalue=1e-67,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_SILST (Q1GHP9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613079.1
- ProteinModelPortal:   Q1GHP9
- SMR:   Q1GHP9
- STRING:   Q1GHP9
- GeneID:   4076317
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1084
- NMPDR:   fig|292414.1.peg.3148
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   EMHETAR
- PhylomeDB:   Q1GHP9
- ProtClustDB:   PRK00073
- BioCyc:   SSP292414:TM1040_1084-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40778; Mature: 40647

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 118-118 BINDING 151-151 BINDING 201-201 BINDING 323-323

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGWKTLDDMDLQGKSVLTRVDINVPMEDGKVTDDTRIRRLVPTISDLLSKGAKPVLLAHF
CCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGKVVPEMSLQPLVPALEAAFGAPVMFVADCRGPAAKGAVDALAPGQILLLENTRFY
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCEEEEEECCCCC
PGEEQNDADLAKEMAALGDIYCNDAFSAAHRAHASTEALARLLPACAGRLMEAELKALEG
CCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLATPERPVTAVVGGAKVSTKLDLLGNLVAKVDTLVIGGGMANTFLAAQGIDVGKSLCEH
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHH
DMTGTAKDIMARAEAAGCTILLPSDVVVAREFKAGADNETVPADACPADAMILDAGPASV
CCCCCHHHHHHHHHHCCEEEECCCCEEEEEHHCCCCCCCCCCCCCCCCCEEEEECCCCHH
AAISTVFAESKTLIWNGPLGAFEIAPFDIATNTAAQKAAELTKAGQLISVAGGGDTVAAL
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
NQAGAAEAFSYISTAGGAFLEWMEGKVLPGVAALQT
HHCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
GWKTLDDMDLQGKSVLTRVDINVPMEDGKVTDDTRIRRLVPTISDLLSKGAKPVLLAHF
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEC
GRPKGKVVPEMSLQPLVPALEAAFGAPVMFVADCRGPAAKGAVDALAPGQILLLENTRFY
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHCCCCEEEEEECCCCC
PGEEQNDADLAKEMAALGDIYCNDAFSAAHRAHASTEALARLLPACAGRLMEAELKALEG
CCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLATPERPVTAVVGGAKVSTKLDLLGNLVAKVDTLVIGGGMANTFLAAQGIDVGKSLCEH
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCHHHHHHHHH
DMTGTAKDIMARAEAAGCTILLPSDVVVAREFKAGADNETVPADACPADAMILDAGPASV
CCCCCHHHHHHHHHHCCEEEECCCCEEEEEHHCCCCCCCCCCCCCCCCCEEEEECCCCHH
AAISTVFAESKTLIWNGPLGAFEIAPFDIATNTAAQKAAELTKAGQLISVAGGGDTVAAL
HHHHHHHHCCCEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHH
NQAGAAEAFSYISTAGGAFLEWMEGKVLPGVAALQT
HHCCHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA