Click here to switch to the map view.

The map label for this gene is rspA [H]

Identifier: 99080687

GI number: 99080687

Start: 897499

End: 898674

Strand: Direct

Name: rspA [H]

Synonym: TM1040_0846

Alternate gene names: 99080687

Gene position: 897499-898674 (Clockwise)

Preceding gene: 99080686

Following gene: 99080688

Centisome position: 28.04

GC content: 63.01

Gene sequence:

>1176_bases
ATGAGCACGCCTTATTCCACGTCCGGCGCAATGCCGGTGACACATGCGCGCGGTGCCGACGACACTGGCCCGCGCATCAG
CAAGATCGAAACCTTCTGCACGCCGCTCATTGGCTTTGTGCGGGTGACTGCGGAGGATGGCAGCCAAGGCTGGGGGCAGG
TCTCCACCTACAACAGCGATCTGACCTCGGAGATCCTGCATCGGCAGGTCGCGCCCTGGGCACTCGGGCGGGGTATGGAT
GCGCTGGAAGAGGTGATTGCCGAAATTCCCCTGCGCGAACATAAATTCCCCGGCACCTATCTGCGCCGCGCCATGGCCGG
TCTCGACACCGCCGTGTGGGACTGGCGCGGCAAAGCTCAGGGCAAGCCGGTGGCAGAGCTTTTGGGTGGGTCTGCAGGGC
CGGTCCGGGCGTATGCCTCCTCCATGCGCCGCGACATCACCCCAGAGGCCGAGGCCGAGCGTATGCAGAGGCTGCGCGAC
GCGCATGGGTTTGACGCCTTCAAAGTCCGTGTAGGGGCTGAATGCGGTCAGGACCGTGACGAATGGGAGGGTCGCACCGA
GGCGATCATTCCCGCCATGTGCAAGGCGATGGGAGCGCAAGCTGCCTTGCTGGTCGACGGCAATTCTGGCTTCAGCCCCG
CCCGCGCGATCGAGGTCGGCAGAATGCTGGAGGCCAACGGATACGAGCATTTTGAAGAACCCTGTCCCTATTGGGAGCAG
GAGCAGACCCGCGAGGTCACCCAAGCGCTTGGGATCGATGTGGCGGGGGGCGAGCAGGACTGCGATCTGCAGCACTGGAA
GCGTATGATCGAGAACCGTGTGGTCGACATCATCCAACCGGATATCCTCTATCTCGGCGGGATGGTGCGCAGCATGGAAG
TGGCCCGCATGGGCCATGCGGCAGGGCTGCCCTGTACGCCCCATGCGGCGAATCTTTCGCTGGTGACGCTCTTCACCATG
CACCTGATGCGTGCGCTGCCCAATCCCGGTCGCTATCTGGAGTTCTCCATCGAAGGCGACGACTACTATCCCTGGCAGCG
CACCTTGTTCGCAAATGATCCCTTTCAGATCACCAATGGCCAGGCGCTTGTCACCGACGCCCCCGGTTGGGGCGTGGAGA
TTTGCCCCGAGTGGCTGGCCAAATCCACCTACAAATGCAGCGAGGACGACCAATGA

Upstream 100 bases:

>100_bases
TCGTTGATGCCTCGGTCTTCCCGAATATCACCTCGGGCAACACCAACGCCCCAACCATGATGCTTGCCACCCGCGCGGCC
GGTCTCATTCTGGAGGACGC

Downstream 100 bases:

>100_bases
GCGTTGACGCCTTAATCTCTACCTATTTCGCCAACGGGCGCCTGTCGGATCTGCCGCGCGATCACTTTATCGACGGTCGC
CTTGTTCCGGCTGAAACCGG

Product: mandelate racemase/muconate lactonizing-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTPYSTSGAMPVTHARGADDTGPRISKIETFCTPLIGFVRVTAEDGSQGWGQVSTYNSDLTSEILHRQVAPWALGRGMD
ALEEVIAEIPLREHKFPGTYLRRAMAGLDTAVWDWRGKAQGKPVAELLGGSAGPVRAYASSMRRDITPEAEAERMQRLRD
AHGFDAFKVRVGAECGQDRDEWEGRTEAIIPAMCKAMGAQAALLVDGNSGFSPARAIEVGRMLEANGYEHFEEPCPYWEQ
EQTREVTQALGIDVAGGEQDCDLQHWKRMIENRVVDIIQPDILYLGGMVRSMEVARMGHAAGLPCTPHAANLSLVTLFTM
HLMRALPNPGRYLEFSIEGDDYYPWQRTLFANDPFQITNGQALVTDAPGWGVEICPEWLAKSTYKCSEDDQ

Sequences:

>Translated_391_residues
MSTPYSTSGAMPVTHARGADDTGPRISKIETFCTPLIGFVRVTAEDGSQGWGQVSTYNSDLTSEILHRQVAPWALGRGMD
ALEEVIAEIPLREHKFPGTYLRRAMAGLDTAVWDWRGKAQGKPVAELLGGSAGPVRAYASSMRRDITPEAEAERMQRLRD
AHGFDAFKVRVGAECGQDRDEWEGRTEAIIPAMCKAMGAQAALLVDGNSGFSPARAIEVGRMLEANGYEHFEEPCPYWEQ
EQTREVTQALGIDVAGGEQDCDLQHWKRMIENRVVDIIQPDILYLGGMVRSMEVARMGHAAGLPCTPHAANLSLVTLFTM
HLMRALPNPGRYLEFSIEGDDYYPWQRTLFANDPFQITNGQALVTDAPGWGVEICPEWLAKSTYKCSEDDQ
>Mature_390_residues
STPYSTSGAMPVTHARGADDTGPRISKIETFCTPLIGFVRVTAEDGSQGWGQVSTYNSDLTSEILHRQVAPWALGRGMDA
LEEVIAEIPLREHKFPGTYLRRAMAGLDTAVWDWRGKAQGKPVAELLGGSAGPVRAYASSMRRDITPEAEAERMQRLRDA
HGFDAFKVRVGAECGQDRDEWEGRTEAIIPAMCKAMGAQAALLVDGNSGFSPARAIEVGRMLEANGYEHFEEPCPYWEQE
QTREVTQALGIDVAGGEQDCDLQHWKRMIENRVVDIIQPDILYLGGMVRSMEVARMGHAAGLPCTPHAANLSLVTLFTMH
LMRALPNPGRYLEFSIEGDDYYPWQRTLFANDPFQITNGQALVTDAPGWGVEICPEWLAKSTYKCSEDDQ

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=25.4950495049505, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI48994953, Length=287, Percent_Identity=25.4355400696864, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789150, Length=243, Percent_Identity=26.7489711934156, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 43212; Mature: 43081

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPYSTSGAMPVTHARGADDTGPRISKIETFCTPLIGFVRVTAEDGSQGWGQVSTYNSD
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHH
LTSEILHRQVAPWALGRGMDALEEVIAEIPLREHKFPGTYLRRAMAGLDTAVWDWRGKAQ
HHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
GKPVAELLGGSAGPVRAYASSMRRDITPEAEAERMQRLRDAHGFDAFKVRVGAECGQDRD
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
EWEGRTEAIIPAMCKAMGAQAALLVDGNSGFSPARAIEVGRMLEANGYEHFEEPCPYWEQ
HHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCH
EQTREVTQALGIDVAGGEQDCDLQHWKRMIENRVVDIIQPDILYLGGMVRSMEVARMGHA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
AGLPCTPHAANLSLVTLFTMHLMRALPNPGRYLEFSIEGDDYYPWQRTLFANDPFQITNG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEECCCCEEEECC
QALVTDAPGWGVEICPEWLAKSTYKCSEDDQ
CEEEECCCCCCHHHCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
STPYSTSGAMPVTHARGADDTGPRISKIETFCTPLIGFVRVTAEDGSQGWGQVSTYNSD
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCHH
LTSEILHRQVAPWALGRGMDALEEVIAEIPLREHKFPGTYLRRAMAGLDTAVWDWRGKAQ
HHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
GKPVAELLGGSAGPVRAYASSMRRDITPEAEAERMQRLRDAHGFDAFKVRVGAECGQDRD
CCHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
EWEGRTEAIIPAMCKAMGAQAALLVDGNSGFSPARAIEVGRMLEANGYEHFEEPCPYWEQ
HHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCH
EQTREVTQALGIDVAGGEQDCDLQHWKRMIENRVVDIIQPDILYLGGMVRSMEVARMGHA
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
AGLPCTPHAANLSLVTLFTMHLMRALPNPGRYLEFSIEGDDYYPWQRTLFANDPFQITNG
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHEEECCCCEEEECC
QALVTDAPGWGVEICPEWLAKSTYKCSEDDQ
CEEEECCCCCCHHHCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]