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The map label for this gene is alkJ [H]

Identifier: 99080686

GI number: 99080686

Start: 895892

End: 897502

Strand: Direct

Name: alkJ [H]

Synonym: TM1040_0845

Alternate gene names: 99080686

Gene position: 895892-897502 (Clockwise)

Preceding gene: 99080684

Following gene: 99080687

Centisome position: 27.99

GC content: 64.25

Gene sequence:

>1611_bases
ATGCGTGACTTTGACTATATCATTGTGGGAGCCGGATCCGCGGGCTGTGTGCTGGCCGAGCGCCTGAGCGCCAATGGCCG
CCATAGTGTGCTTGTGCTGGAGGCCGGAGGTCGCCCGCGCACACCATGGATCGCGTTGCCGCTTGGCTACGGCAAGACCT
TCTATGACCCGGCGGTGAACTGGAAATATCAGACTGAACCCGAAGAGACACTGGGCGGACGCGCCGGATATTGGCCGCGT
GGCAAGGTCGTGGGGGGATCGGGTGCGATCAATGCGCTTGTCTACGCCCGTGGCCTGGCGCGCGATTTCGACGATTGGGA
AGAGGCGGGCGCGACGGGCTGGAACTGGGACGCGGTCCAGAAAACCTATGAGCGCCTTGAGAGCCGCTTTGATGTCGATG
GCACCCGCACCGGCGAGGGGCCGATTCACGTTCAGGATGTCTCGGACCAGATCCACCGGGCCAACCGGCATTTCTTTGCC
GCAGCGAAAGAGCTGGGTCTGCCACGGACACCCGATATGAACGGTATCACCCCCGAAGGCGCGGGCGTCTACCGGATCAA
CACCAGCGGTGGGCGCAGGATGCATTCGGCGCGCGCCTGTTTGGCTCCTGCGCTCCGGCGCGCAAATGTGACGCTGATGA
CGGGCGTTCTGGTGGAGCGGATCGGCTTTGAGGGAAAGCGGGCCACCTCCGTCGAGGTGGTCCACAAGGGGCGCGCGCAG
TCCTTGCAGGCCGGGCGAGAGATCATTCTCGCGGCAGGGGCTGTAAATTCACCGCGCATCTTGCAACTCTCGGGGCTTGG
CCCCGCGGAGCTGCTGCGTGAGCATGGGATCGCGCCGCTGATGGATGCGCCTCATGTAGGTGGCAACCTGCAGGATCATC
TGGGCATAAACTATTATTTCCGTGCCACCGAACCCACGCTCAACAACGTGCTGAGGCCGCTCCATGGCAAGATCCGCGCA
GCGCTGCAATATGCGCTCACGCGGCGCGGGCCGCTCGCGCTCTCGGTCAACCAATGTGGTGGATTTTTTCGCTCGGATGC
GGGGCAGCGGGCGGCTGATCAGCAGCTTTACTTCAACCCCGTGACCTATACCACCACACCGGACGGCAAACGCACGGTGG
TGCAGCCCGACCCCTTTGCGGGCTTTATCCTTGGGTTTCAGCCCACCCGGCCCATCAGCCGGGGGCGAATCGACATTTCC
GCCGCCGACGCGCTTGCGCCGCCCCGGATCAGGCCGGACTCGCTGGCTGCTCAGGAAGATCAGGCGCAGGTGATCGCAGG
CGGGCTGCTCTGTCAGAAGATCGCCAAGACCGAGGCGCTCAGCCGCTTGATCGCCGCGCCCATGGGCGAGGATCTGCGCG
AGATGACACCGGAGCAGATCCTAGCGGACTTTCGCGAGCGCTGCGGCACCGTGTTTCACCCGGTCGGCACCTGTCGCATG
GGTGCAGACAGCACCAAGTCCGTGGTTTGCCCTCGGCTCAAGGTGCATGGGGTCGCGGGGCTGCGGGTCGTTGATGCCTC
GGTCTTCCCGAATATCACCTCGGGCAACACCAACGCCCCAACCATGATGCTTGCCACCCGCGCGGCCGGTCTCATTCTGG
AGGACGCATGA

Upstream 100 bases:

>100_bases
AATCTGCGAATATTTTTAAGGTTACGGCGAATTATTTTTCGTTATGGGGATTGGAGCCGGAGCTGTAACGCTGGCGCGAT
GAATGGAGACCAACATCGCG

Downstream 100 bases:

>100_bases
GCACGCCTTATTCCACGTCCGGCGCAATGCCGGTGACACATGCGCGCGGTGCCGACGACACTGGCCCGCGCATCAGCAAG
ATCGAAACCTTCTGCACGCC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MRDFDYIIVGAGSAGCVLAERLSANGRHSVLVLEAGGRPRTPWIALPLGYGKTFYDPAVNWKYQTEPEETLGGRAGYWPR
GKVVGGSGAINALVYARGLARDFDDWEEAGATGWNWDAVQKTYERLESRFDVDGTRTGEGPIHVQDVSDQIHRANRHFFA
AAKELGLPRTPDMNGITPEGAGVYRINTSGGRRMHSARACLAPALRRANVTLMTGVLVERIGFEGKRATSVEVVHKGRAQ
SLQAGREIILAAGAVNSPRILQLSGLGPAELLREHGIAPLMDAPHVGGNLQDHLGINYYFRATEPTLNNVLRPLHGKIRA
ALQYALTRRGPLALSVNQCGGFFRSDAGQRAADQQLYFNPVTYTTTPDGKRTVVQPDPFAGFILGFQPTRPISRGRIDIS
AADALAPPRIRPDSLAAQEDQAQVIAGGLLCQKIAKTEALSRLIAAPMGEDLREMTPEQILADFRERCGTVFHPVGTCRM
GADSTKSVVCPRLKVHGVAGLRVVDASVFPNITSGNTNAPTMMLATRAAGLILEDA

Sequences:

>Translated_536_residues
MRDFDYIIVGAGSAGCVLAERLSANGRHSVLVLEAGGRPRTPWIALPLGYGKTFYDPAVNWKYQTEPEETLGGRAGYWPR
GKVVGGSGAINALVYARGLARDFDDWEEAGATGWNWDAVQKTYERLESRFDVDGTRTGEGPIHVQDVSDQIHRANRHFFA
AAKELGLPRTPDMNGITPEGAGVYRINTSGGRRMHSARACLAPALRRANVTLMTGVLVERIGFEGKRATSVEVVHKGRAQ
SLQAGREIILAAGAVNSPRILQLSGLGPAELLREHGIAPLMDAPHVGGNLQDHLGINYYFRATEPTLNNVLRPLHGKIRA
ALQYALTRRGPLALSVNQCGGFFRSDAGQRAADQQLYFNPVTYTTTPDGKRTVVQPDPFAGFILGFQPTRPISRGRIDIS
AADALAPPRIRPDSLAAQEDQAQVIAGGLLCQKIAKTEALSRLIAAPMGEDLREMTPEQILADFRERCGTVFHPVGTCRM
GADSTKSVVCPRLKVHGVAGLRVVDASVFPNITSGNTNAPTMMLATRAAGLILEDA
>Mature_536_residues
MRDFDYIIVGAGSAGCVLAERLSANGRHSVLVLEAGGRPRTPWIALPLGYGKTFYDPAVNWKYQTEPEETLGGRAGYWPR
GKVVGGSGAINALVYARGLARDFDDWEEAGATGWNWDAVQKTYERLESRFDVDGTRTGEGPIHVQDVSDQIHRANRHFFA
AAKELGLPRTPDMNGITPEGAGVYRINTSGGRRMHSARACLAPALRRANVTLMTGVLVERIGFEGKRATSVEVVHKGRAQ
SLQAGREIILAAGAVNSPRILQLSGLGPAELLREHGIAPLMDAPHVGGNLQDHLGINYYFRATEPTLNNVLRPLHGKIRA
ALQYALTRRGPLALSVNQCGGFFRSDAGQRAADQQLYFNPVTYTTTPDGKRTVVQPDPFAGFILGFQPTRPISRGRIDIS
AADALAPPRIRPDSLAAQEDQAQVIAGGLLCQKIAKTEALSRLIAAPMGEDLREMTPEQILADFRERCGTVFHPVGTCRM
GADSTKSVVCPRLKVHGVAGLRVVDASVFPNITSGNTNAPTMMLATRAAGLILEDA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=546, Percent_Identity=36.6300366300366, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1786503, Length=542, Percent_Identity=35.4243542435424, Blast_Score=265, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17532301, Length=559, Percent_Identity=34.8837209302326, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI45549471, Length=576, Percent_Identity=32.9861111111111, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI45551458, Length=578, Percent_Identity=32.8719723183391, Blast_Score=268, Evalue=8e-72,
Organism=Drosophila melanogaster, GI24642055, Length=570, Percent_Identity=32.8070175438597, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI18859993, Length=581, Percent_Identity=31.3253012048193, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24650267, Length=570, Percent_Identity=32.280701754386, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24642042, Length=571, Percent_Identity=31.6987740805604, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24642059, Length=573, Percent_Identity=31.9371727748691, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24642051, Length=566, Percent_Identity=30.2120141342756, Blast_Score=242, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24642037, Length=577, Percent_Identity=33.4488734835355, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24642048, Length=567, Percent_Identity=31.7460317460317, Blast_Score=239, Evalue=4e-63,
Organism=Drosophila melanogaster, GI24642039, Length=574, Percent_Identity=32.2299651567944, Blast_Score=237, Evalue=2e-62,
Organism=Drosophila melanogaster, GI18859995, Length=570, Percent_Identity=33.1578947368421, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24642035, Length=586, Percent_Identity=32.4232081911263, Blast_Score=226, Evalue=3e-59,
Organism=Drosophila melanogaster, GI17137792, Length=561, Percent_Identity=29.9465240641711, Blast_Score=219, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24642057, Length=590, Percent_Identity=31.6949152542373, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24645930, Length=585, Percent_Identity=27.3504273504274, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 57840; Mature: 57840

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDFDYIIVGAGSAGCVLAERLSANGRHSVLVLEAGGRPRTPWIALPLGYGKTFYDPAVN
CCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCC
WKYQTEPEETLGGRAGYWPRGKVVGGSGAINALVYARGLARDFDDWEEAGATGWNWDAVQ
CEECCCCHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
KTYERLESRFDVDGTRTGEGPIHVQDVSDQIHRANRHFFAAAKELGLPRTPDMNGITPEG
HHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AGVYRINTSGGRRMHSARACLAPALRRANVTLMTGVLVERIGFEGKRATSVEVVHKGRAQ
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHH
SLQAGREIILAAGAVNSPRILQLSGLGPAELLREHGIAPLMDAPHVGGNLQDHLGINYYF
HHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEE
RATEPTLNNVLRPLHGKIRAALQYALTRRGPLALSVNQCGGFFRSDAGQRAADQQLYFNP
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHCCCCCCCCCCCEEECC
VTYTTTPDGKRTVVQPDPFAGFILGFQPTRPISRGRIDISAADALAPPRIRPDSLAAQED
EEEEECCCCCEEEECCCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCH
QAQVIAGGLLCQKIAKTEALSRLIAAPMGEDLREMTPEQILADFRERCGTVFHPVGTCRM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCCEEC
GADSTKSVVCPRLKVHGVAGLRVVDASVFPNITSGNTNAPTMMLATRAAGLILEDA
CCCCCCCEECCCEEECCCCCEEEEEHHHCCCCCCCCCCCCEEEEEEHHCCEEEECC
>Mature Secondary Structure
MRDFDYIIVGAGSAGCVLAERLSANGRHSVLVLEAGGRPRTPWIALPLGYGKTFYDPAVN
CCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCC
WKYQTEPEETLGGRAGYWPRGKVVGGSGAINALVYARGLARDFDDWEEAGATGWNWDAVQ
CEECCCCHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
KTYERLESRFDVDGTRTGEGPIHVQDVSDQIHRANRHFFAAAKELGLPRTPDMNGITPEG
HHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
AGVYRINTSGGRRMHSARACLAPALRRANVTLMTGVLVERIGFEGKRATSVEVVHKGRAQ
CEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCHH
SLQAGREIILAAGAVNSPRILQLSGLGPAELLREHGIAPLMDAPHVGGNLQDHLGINYYF
HHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCHHHCCEEEEE
RATEPTLNNVLRPLHGKIRAALQYALTRRGPLALSVNQCGGFFRSDAGQRAADQQLYFNP
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHCCCCCCCCCCCEEECC
VTYTTTPDGKRTVVQPDPFAGFILGFQPTRPISRGRIDISAADALAPPRIRPDSLAAQED
EEEEECCCCCEEEECCCCCCEEEEECCCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCCH
QAQVIAGGLLCQKIAKTEALSRLIAAPMGEDLREMTPEQILADFRERCGTVFHPVGTCRM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCCEEC
GADSTKSVVCPRLKVHGVAGLRVVDASVFPNITSGNTNAPTMMLATRAAGLILEDA
CCCCCCCEECCCEEECCCCCEEEEEHHHCCCCCCCCCCCCEEEEEEHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]