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The map label for this gene is nemA [H]

Identifier: 99080614

GI number: 99080614

Start: 827885

End: 828559

Strand: Direct

Name: nemA [H]

Synonym: TM1040_0773

Alternate gene names: 99080614

Gene position: 827885-828559 (Clockwise)

Preceding gene: 99080611

Following gene: 99080615

Centisome position: 25.86

GC content: 61.33

Gene sequence:

>675_bases
GTGAGCCAGAAACTGTTCGCCGAACCAACCGCCGGTGCCCTTGAGTTGACAACTCGCTTGGTGATTGCACCGCTGAAGCA
CGATCGTGCCGGTGACACCACCGCAGAGGTGCTTGAGCGTTACGCCGAATACTACGCCCAACGCGCCAGCGCCGGTCTGA
TCATCACCGAAGGCAGCCAGATCAGCGCTGAGGCCAGAGGGTACATCCAGACCCCCGGCATCCACACCGCCGCGCAGGCT
GCTGCATGGCGCAAGGTCACTGATGCGGTGCATGCCAAGGGCGGCAAAATCGTGATCCAGATGTGGCACGTGGGGCGTAG
CACACATGCCGGGTTTGAACCCACCGCGACGCCCGAGGCGCTGACGGTTGGAGGCATTCAGCGCGTGATCGAAGATCGCG
TTCAGGCCGCGACATACGCCAAAGAGGCAGGATTTGACGGCGTTGAGTTGCATGTCGCCAATGGTTGTTTGCTGGAGCAA
TTCCGAAAGGATAGGGTCAACACCCGCAGCGATGCCTATGGCGGATCGGCAGAAAATCGGGCGCGTTTGCTGTTTGAGGT
TCTGGATGCCTTGGCCACCGTCTGGGAGCCGGGGCGCATTGCCGTGCGTCTGGCACCCTTTGCCAGCTTAACGATGTCTT
CGATAGCGCCCCCATGGCCCATTCCACCCCGCTGA

Upstream 100 bases:

>100_bases
GAACATTCTCGTTGCTGGAGGGGTGAGAGGCGCAGGGAATGCTCTGTTCAAGTGGAGCAATACGCCTGATGCAGTATACC
TTGAGCGACGGATACTCCCC

Downstream 100 bases:

>100_bases
TCGAACGACTGAACACCCATGGTCTGGCCTGATTGTACCTGATCAGAGGCGACACCGGTGGCGCGCTTAGGGGATTTTGA
GATCCTCCGCCAGCTTTATA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSQKLFAEPTAGALELTTRLVIAPLKHDRAGDTTAEVLERYAEYYAQRASAGLIITEGSQISAEARGYIQTPGIHTAAQA
AAWRKVTDAVHAKGGKIVIQMWHVGRSTHAGFEPTATPEALTVGGIQRVIEDRVQAATYAKEAGFDGVELHVANGCLLEQ
FRKDRVNTRSDAYGGSAENRARLLFEVLDALATVWEPGRIAVRLAPFASLTMSSIAPPWPIPPR

Sequences:

>Translated_224_residues
MSQKLFAEPTAGALELTTRLVIAPLKHDRAGDTTAEVLERYAEYYAQRASAGLIITEGSQISAEARGYIQTPGIHTAAQA
AAWRKVTDAVHAKGGKIVIQMWHVGRSTHAGFEPTATPEALTVGGIQRVIEDRVQAATYAKEAGFDGVELHVANGCLLEQ
FRKDRVNTRSDAYGGSAENRARLLFEVLDALATVWEPGRIAVRLAPFASLTMSSIAPPWPIPPR
>Mature_223_residues
SQKLFAEPTAGALELTTRLVIAPLKHDRAGDTTAEVLERYAEYYAQRASAGLIITEGSQISAEARGYIQTPGIHTAAQAA
AWRKVTDAVHAKGGKIVIQMWHVGRSTHAGFEPTATPEALTVGGIQRVIEDRVQAATYAKEAGFDGVELHVANGCLLEQF
RKDRVNTRSDAYGGSAENRARLLFEVLDALATVWEPGRIAVRLAPFASLTMSSIAPPWPIPPR

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=249, Percent_Identity=40.9638554216867, Blast_Score=181, Evalue=2e-47,
Organism=Escherichia coli, GI1789463, Length=165, Percent_Identity=34.5454545454545, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=194, Percent_Identity=31.4432989690722, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17565138, Length=187, Percent_Identity=33.1550802139037, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=117, Percent_Identity=41.025641025641, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=121, Percent_Identity=37.1900826446281, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17564188, Length=128, Percent_Identity=35.9375, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001454, Length=187, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17566914, Length=183, Percent_Identity=29.5081967213115, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=247, Percent_Identity=35.2226720647773, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321973, Length=243, Percent_Identity=34.9794238683128, Blast_Score=119, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24202; Mature: 24071

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLFAEPTAGALELTTRLVIAPLKHDRAGDTTAEVLERYAEYYAQRASAGLIITEGSQ
CCCCCCCCCCCCHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
ISAEARGYIQTPGIHTAAQAAAWRKVTDAVHAKGGKIVIQMWHVGRSTHAGFEPTATPEA
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCH
LTVGGIQRVIEDRVQAATYAKEAGFDGVELHVANGCLLEQFRKDRVNTRSDAYGGSAENR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHH
ARLLFEVLDALATVWEPGRIAVRLAPFASLTMSSIAPPWPIPPR
HHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SQKLFAEPTAGALELTTRLVIAPLKHDRAGDTTAEVLERYAEYYAQRASAGLIITEGSQ
CCCCCCCCCCCHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCC
ISAEARGYIQTPGIHTAAQAAAWRKVTDAVHAKGGKIVIQMWHVGRSTHAGFEPTATPEA
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCH
LTVGGIQRVIEDRVQAATYAKEAGFDGVELHVANGCLLEQFRKDRVNTRSDAYGGSAENR
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCHHH
ARLLFEVLDALATVWEPGRIAVRLAPFASLTMSSIAPPWPIPPR
HHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]