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The map label for this gene is tgt

Identifier: 99080611

GI number: 99080611

Start: 825392

End: 826522

Strand: Direct

Name: tgt

Synonym: TM1040_0770

Alternate gene names: 99080611

Gene position: 825392-826522 (Clockwise)

Preceding gene: 99080604

Following gene: 99080614

Centisome position: 25.79

GC content: 60.3

Gene sequence:

>1131_bases
ATGGCAGAGCTTTTCAACTTTGAAATGACAGCAACGGACGGCAAAGCACGCACGGGTGTAATCCATACACCACGCGGCGA
GATCCGTACCCCTGCTTTCATGCCGGTAGGCACTGCTGCGACCGTCAAGGCGATGATGCCCGAAAGCGTGCGCGCAACCG
GAGCGGACATTCTGTTGGGCAATACCTATCACCTGATGCTGCGCCCGACTGCCGAACGGATCGACCGTCTGGGGGGCTTG
CACAAATTCATGAACTGGGACCGCCCGATCCTCACCGACTCCGGCGGGTTTCAGGTGATGTCGCTCGCGGGTCTGCGCAA
GCTCACCGAAAAAGGCGTGACGTTCAAATCTCATATCGATGGGTCGCGCCACGAGCTGACGCCGGAACGCTCGATGGAGA
TTCAACGACTGCTGGGCTCTGACATTGTGATGTGTTTCGACGAATGCCCCGCGCTGCCTGCCGACCGGGATCGCATCGCC
GAGAGCATGCGTCTGTCGATGCGCTGGGCGGAACGCTCGCGCGAGGCCTTTGGCGACCGGCCGGGACATGCGCTCTTTGG
CATTCAGCAGGGTGGGCTGGAACAGGATTTCCGCGAGGAAAGCGCAGAAGCCCTCACCAAGATTGGCTTTGATGGCTACG
CGGTGGGTGGTCTTGCGGTGGGCGAGGGGCAAGAGGCCATGTTTGGCTGTCTGGATTACGCGCCCGACATGCTGCCGGTT
GATAAGCCGCGCTATCTGATGGGCGTCGGCAAGCCCGATGATATCGTGGGCGCGGTGTCCCGTGGTATCGACATGATGGA
CTGCGTGTTGCCGTCGCGCTCTGGTCGCACGGGGCAGGCGTTCACTCGGCATGGTGTGGTCAATATCAAGAATGCGCGCC
ATCAGGACGACCCGCGCCCGCTGGATGAGAATTGCAGCTGTCCGGCATGCAGCAATTATTCGCGCGCCTATCTGCACCAC
GTGTTCCGGTCGAACGAGATGATCTCCGGCATGCTTTTGACATGGCACAACCTGCATTACTTCCAAGACATCATGGCTGG
CATGCGGGAGTCAATTGCAGCGGGCACATTTGAGGCGTGGCAGAAGACGTTCCACGAGACGCGCGCCCAAGGCGATATCG
AGCCGCTCTGA

Upstream 100 bases:

>100_bases
TAGGTTCTGGTTCGGGTCATCTGTGGACATGAGGACGCCTCCTTGGCATTAGGGGTGAATCACCGCACACGCGGTGCAGA
GTTTGATGCAGGAACATGAG

Downstream 100 bases:

>100_bases
GCCTGATCCTCGTCGGGGCCAGAAGCTGACACATTCTGTTTCATATATCGCATCACCACCCGCAAAAGAGGTCCGGGTGG
TGATGCGTGTATTAGCTCTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAELFNFEMTATDGKARTGVIHTPRGEIRTPAFMPVGTAATVKAMMPESVRATGADILLGNTYHLMLRPTAERIDRLGGL
HKFMNWDRPILTDSGGFQVMSLAGLRKLTEKGVTFKSHIDGSRHELTPERSMEIQRLLGSDIVMCFDECPALPADRDRIA
ESMRLSMRWAERSREAFGDRPGHALFGIQQGGLEQDFREESAEALTKIGFDGYAVGGLAVGEGQEAMFGCLDYAPDMLPV
DKPRYLMGVGKPDDIVGAVSRGIDMMDCVLPSRSGRTGQAFTRHGVVNIKNARHQDDPRPLDENCSCPACSNYSRAYLHH
VFRSNEMISGMLLTWHNLHYFQDIMAGMRESIAAGTFEAWQKTFHETRAQGDIEPL

Sequences:

>Translated_376_residues
MAELFNFEMTATDGKARTGVIHTPRGEIRTPAFMPVGTAATVKAMMPESVRATGADILLGNTYHLMLRPTAERIDRLGGL
HKFMNWDRPILTDSGGFQVMSLAGLRKLTEKGVTFKSHIDGSRHELTPERSMEIQRLLGSDIVMCFDECPALPADRDRIA
ESMRLSMRWAERSREAFGDRPGHALFGIQQGGLEQDFREESAEALTKIGFDGYAVGGLAVGEGQEAMFGCLDYAPDMLPV
DKPRYLMGVGKPDDIVGAVSRGIDMMDCVLPSRSGRTGQAFTRHGVVNIKNARHQDDPRPLDENCSCPACSNYSRAYLHH
VFRSNEMISGMLLTWHNLHYFQDIMAGMRESIAAGTFEAWQKTFHETRAQGDIEPL
>Mature_375_residues
AELFNFEMTATDGKARTGVIHTPRGEIRTPAFMPVGTAATVKAMMPESVRATGADILLGNTYHLMLRPTAERIDRLGGLH
KFMNWDRPILTDSGGFQVMSLAGLRKLTEKGVTFKSHIDGSRHELTPERSMEIQRLLGSDIVMCFDECPALPADRDRIAE
SMRLSMRWAERSREAFGDRPGHALFGIQQGGLEQDFREESAEALTKIGFDGYAVGGLAVGEGQEAMFGCLDYAPDMLPVD
KPRYLMGVGKPDDIVGAVSRGIDMMDCVLPSRSGRTGQAFTRHGVVNIKNARHQDDPRPLDENCSCPACSNYSRAYLHHV
FRSNEMISGMLLTWHNLHYFQDIMAGMRESIAAGTFEAWQKTFHETRAQGDIEPL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=350, Percent_Identity=42.5714285714286, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=24.3150684931507, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=54.4235924932976, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17544680, Length=359, Percent_Identity=40.9470752089136, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25146502, Length=154, Percent_Identity=27.9220779220779, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19920510, Length=351, Percent_Identity=43.8746438746439, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=25.0980392156863, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_SILST (Q1GIL3)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_612765.1
- ProteinModelPortal:   Q1GIL3
- SMR:   Q1GIL3
- STRING:   Q1GIL3
- GeneID:   4078082
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_0770
- NMPDR:   fig|292414.1.peg.2873
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   KAMLPEN
- PhylomeDB:   Q1GIL3
- ProtClustDB:   PRK00112
- BioCyc:   SSP292414:TM1040_0770-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41744; Mature: 41613

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELFNFEMTATDGKARTGVIHTPRGEIRTPAFMPVGTAATVKAMMPESVRATGADILLG
CCCEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEC
NTYHLMLRPTAERIDRLGGLHKFMNWDRPILTDSGGFQVMSLAGLRKLTEKGVTFKSHID
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHHHHHHHHHCCCEEEHHCC
GSRHELTPERSMEIQRLLGSDIVMCFDECPALPADRDRIAESMRLSMRWAERSREAFGDR
CCHHCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PGHALFGIQQGGLEQDFREESAEALTKIGFDGYAVGGLAVGEGQEAMFGCLDYAPDMLPV
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
DKPRYLMGVGKPDDIVGAVSRGIDMMDCVLPSRSGRTGQAFTRHGVVNIKNARHQDDPRP
CCCCEEEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCC
LDENCSCPACSNYSRAYLHHVFRSNEMISGMLLTWHNLHYFQDIMAGMRESIAAGTFEAW
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
QKTFHETRAQGDIEPL
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AELFNFEMTATDGKARTGVIHTPRGEIRTPAFMPVGTAATVKAMMPESVRATGADILLG
CCEEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCHHHHHCCCEEEEC
NTYHLMLRPTAERIDRLGGLHKFMNWDRPILTDSGGFQVMSLAGLRKLTEKGVTFKSHID
CEEEEEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHHHHHHHHHCCCEEEHHCC
GSRHELTPERSMEIQRLLGSDIVMCFDECPALPADRDRIAESMRLSMRWAERSREAFGDR
CCHHCCCCHHHHHHHHHHCCHHEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
PGHALFGIQQGGLEQDFREESAEALTKIGFDGYAVGGLAVGEGQEAMFGCLDYAPDMLPV
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHCCCCCCC
DKPRYLMGVGKPDDIVGAVSRGIDMMDCVLPSRSGRTGQAFTRHGVVNIKNARHQDDPRP
CCCCEEEECCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHCCCEEECCCCCCCCCCC
LDENCSCPACSNYSRAYLHHVFRSNEMISGMLLTWHNLHYFQDIMAGMRESIAAGTFEAW
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
QKTFHETRAQGDIEPL
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA