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The map label for this gene is echA8 [H]

Identifier: 99080581

GI number: 99080581

Start: 798693

End: 799478

Strand: Direct

Name: echA8 [H]

Synonym: TM1040_0740

Alternate gene names: 99080581

Gene position: 798693-799478 (Clockwise)

Preceding gene: 99080580

Following gene: 99080582

Centisome position: 24.95

GC content: 63.99

Gene sequence:

>786_bases
ATGTATGACACGCTTTTGCTGACCCGTGATGATCGCGGTGTGGCGACCCTTACGCTCAACCGTCCCGAAAAGCACAACGC
CATGTCGGGCCTGATGCTGCAGGAACTGCAGGATGCGGCGCGCGCGTTGGCGGCGGACCGCAGCGTGCGGGTTGTTGTAC
TCACGGGGGCGGGCAAGAGCTTTTGCGCCGGGGGCGATCTGGGCTGGATGCAGGCGCAGATGGAGGCTGATGCCGAAACG
CGCGGGCGCGAGGCCCGCGTGCTGGCCGAGATGCTGAACCTTCTCAACACCTTGCCGCAGCCGCTCATTGGGCGGCTGCA
GGGCAATGCCTTCGGGGGCGGGGTCGGAATGGCCTCGGTCTGTGATGTGGCCATCGGTGTCGACAGTCTCAAGATGGGGC
TCACCGAGACCAAACTGGGACTGATCCCCGCCACCATCGGCCCCTATGTGATCGCCCGCATGGGCGAGGCGCGGGCACGT
CGCGTATTCATGTCGGCGCGACTTTTTGATGCAGCCGAGGCGGTCGATCTGGGGCTCCTGAGCCGCGCGGTACCCGCCGA
CTCACTTGATGCTGCGGTGGAAGCAGAGGTAACTCCTTACCTCGACTGCGCGCCGGGGGCGGTGGCGGATGCCAAGGCGC
TGGCGCGCGCGCTTGGGCCGCGTATCGATAAATCCACAATCGATTTTACAATTTCCGCCCTTGTAACCCGCTGGGAAAGC
GCCGAGGCGGTCGAAGGCATCAACGCGTTTTTTGATAAACGCCGCCCCTCCTGGCGTGACAAATAA

Upstream 100 bases:

>100_bases
TTGATGGCACCGTACTGCAAGAGGCCGCCGCCATGGCGCGGCACTTGCGCCCCGAGCCCACCGACGCCTGAGCTATCAAG
ACACGAAGGACCCTTTGGCC

Downstream 100 bases:

>100_bases
GGGGAATTCCCCTCTGCGAGCGCAGCACGTTTGCGCTCGTTTTTGATGCACACAATCCTTAGCGAAACCATAAGGATGGT
GTGGCCTGTTGCAGCACTGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MYDTLLLTRDDRGVATLTLNRPEKHNAMSGLMLQELQDAARALAADRSVRVVVLTGAGKSFCAGGDLGWMQAQMEADAET
RGREARVLAEMLNLLNTLPQPLIGRLQGNAFGGGVGMASVCDVAIGVDSLKMGLTETKLGLIPATIGPYVIARMGEARAR
RVFMSARLFDAAEAVDLGLLSRAVPADSLDAAVEAEVTPYLDCAPGAVADAKALARALGPRIDKSTIDFTISALVTRWES
AEAVEGINAFFDKRRPSWRDK

Sequences:

>Translated_261_residues
MYDTLLLTRDDRGVATLTLNRPEKHNAMSGLMLQELQDAARALAADRSVRVVVLTGAGKSFCAGGDLGWMQAQMEADAET
RGREARVLAEMLNLLNTLPQPLIGRLQGNAFGGGVGMASVCDVAIGVDSLKMGLTETKLGLIPATIGPYVIARMGEARAR
RVFMSARLFDAAEAVDLGLLSRAVPADSLDAAVEAEVTPYLDCAPGAVADAKALARALGPRIDKSTIDFTISALVTRWES
AEAVEGINAFFDKRRPSWRDK
>Mature_261_residues
MYDTLLLTRDDRGVATLTLNRPEKHNAMSGLMLQELQDAARALAADRSVRVVVLTGAGKSFCAGGDLGWMQAQMEADAET
RGREARVLAEMLNLLNTLPQPLIGRLQGNAFGGGVGMASVCDVAIGVDSLKMGLTETKLGLIPATIGPYVIARMGEARAR
RVFMSARLFDAAEAVDLGLLSRAVPADSLDAAVEAEVTPYLDCAPGAVADAKALARALGPRIDKSTIDFTISALVTRWES
AEAVEGINAFFDKRRPSWRDK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=24.6153846153846, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4502327, Length=265, Percent_Identity=30.188679245283, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI260274832, Length=255, Percent_Identity=24.7058823529412, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI45643119, Length=255, Percent_Identity=24.7058823529412, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI260275230, Length=255, Percent_Identity=24.7058823529412, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI70995211, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=25.0996015936255, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136756, Length=221, Percent_Identity=26.6968325791855, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221136753, Length=221, Percent_Identity=26.6968325791855, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI221307494, Length=221, Percent_Identity=26.6968325791855, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=28.8770053475936, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI25453481, Length=257, Percent_Identity=22.1789883268482, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI51538573, Length=257, Percent_Identity=22.1789883268482, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4757966, Length=250, Percent_Identity=22.8, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538581, Length=250, Percent_Identity=22.8, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757968, Length=258, Percent_Identity=22.093023255814, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI49355787, Length=258, Percent_Identity=22.093023255814, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694516, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI213417737, Length=195, Percent_Identity=25.6410256410256, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1788597, Length=267, Percent_Identity=28.4644194756554, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1787659, Length=265, Percent_Identity=30.5660377358491, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=30.2681992337165, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=29.8507462686567, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=24.0157480314961, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788682, Length=188, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790281, Length=188, Percent_Identity=26.5957446808511, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=32.1266968325792, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=29.8165137614679, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=256, Percent_Identity=26.953125, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=181, Percent_Identity=28.7292817679558, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=261, Percent_Identity=27.5862068965517, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=185, Percent_Identity=28.6486486486486, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=172, Percent_Identity=29.0697674418605, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=256, Percent_Identity=26.5625, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=256, Percent_Identity=26.5625, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=26.8292682926829, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=24.5098039215686, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=32.8125, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=27.6923076923077, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=27.6923076923077, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922422, Length=267, Percent_Identity=26.9662921348315, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920382, Length=207, Percent_Identity=28.5024154589372, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24654903, Length=216, Percent_Identity=24.537037037037, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357171, Length=181, Percent_Identity=26.5193370165746, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=29.8913043478261, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27832; Mature: 27832

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDTLLLTRDDRGVATLTLNRPEKHNAMSGLMLQELQDAARALAADRSVRVVVLTGAGKS
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FCAGGDLGWMQAQMEADAETRGREARVLAEMLNLLNTLPQPLIGRLQGNAFGGGVGMASV
CCCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHH
CDVAIGVDSLKMGLTETKLGLIPATIGPYVIARMGEARARRVFMSARLFDAAEAVDLGLL
HHHHHCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAVPADSLDAAVEAEVTPYLDCAPGAVADAKALARALGPRIDKSTIDFTISALVTRWES
HHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
AEAVEGINAFFDKRRPSWRDK
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MYDTLLLTRDDRGVATLTLNRPEKHNAMSGLMLQELQDAARALAADRSVRVVVLTGAGKS
CCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
FCAGGDLGWMQAQMEADAETRGREARVLAEMLNLLNTLPQPLIGRLQGNAFGGGVGMASV
CCCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHH
CDVAIGVDSLKMGLTETKLGLIPATIGPYVIARMGEARARRVFMSARLFDAAEAVDLGLL
HHHHHCHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRAVPADSLDAAVEAEVTPYLDCAPGAVADAKALARALGPRIDKSTIDFTISALVTRWES
HHCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
AEAVEGINAFFDKRRPSWRDK
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]