Click here to switch to the map view.

The map label for this gene is mvaB [H]

Identifier: 99080580

GI number: 99080580

Start: 797797

End: 798663

Strand: Direct

Name: mvaB [H]

Synonym: TM1040_0739

Alternate gene names: 99080580

Gene position: 797797-798663 (Clockwise)

Preceding gene: 99080579

Following gene: 99080581

Centisome position: 24.92

GC content: 61.94

Gene sequence:

>867_bases
ATGAAAGACCGCGTCGAGATCTTTGAGGTGGGCCCACGCGACGGGCTGCAGAACGAAAAGCGTGACATTCCTGTCGCCGA
CAAGGTGGCCCTGGTGGATTGTCTCAGTCGTGCAGGCTTTTCCCGGATCGAGGTGGCGAGTTTTGTCTCCCCCAAATGGG
TGCCGCAGATGGCGGGCAGCGCAGAGGTTCTGGCCGGGATCACCAAGGCCAAAGGCGTGCGCTATGCGGCGCTCACCCCG
AATATGCGCGGCTATGAGGATGCGCTGGCCGCGCGCGCTGATGAAATCGCCATCTTTGCCTCTGCCTCCGAGGGCTTTTC
ACAAGCCAATATCAATGCATCGATTGCTGAGAGTATCGAGCGCTTTACACCCATTCTGGAGGCCGCGCGTCATATCGATC
TGCCGGTGCGGGGTTATGTCTCCTGCGTGACCGACTGCCCCTATGATGGCGCTGTCGCACCCGACAAAGTCGCCGAGGTC
GCGGATCGGCTGTTCTCGATGGGCTGCTATGAGATTTCGCTTGGCGATACTGTGGGAAAGGCCACACCCGACAAGATCGC
CAAGATGTTGATCGCGGTCAAAGATGCGGTGCCAGTCACCCGCCTCGCAGGTCACTATCACGACACCGCAGGCCGTGCGA
TTGCCAATATAGATGCGTCGCTTGCGCTGGGCGTGCGGGTGTTTGACGCTGCCGTTGGCGGGCTTGGTGGCTGCCCCTAT
GCGCCGGGCGCGGCAGGGAATGTGGCCACAGAGGTGGTCCACGAACATTTGACGCGGCTGGGGTATGACACGGGCCTTGA
TGGCACCGTACTGCAAGAGGCCGCCGCCATGGCGCGGCACTTGCGCCCCGAGCCCACCGACGCCTGA

Upstream 100 bases:

>100_bases
TTCTTGCGCAAGCGGGCGATCAGGTGGAGGCTGGTGCAGCGCTGGTGCGGCTCGAGGAAGAGGCCTGAGCCGCGCGCATG
AACCGGGAGGAGTAAAACCC

Downstream 100 bases:

>100_bases
GCTATCAAGACACGAAGGACCCTTTGGCCATGTATGACACGCTTTTGCTGACCCGTGATGATCGCGGTGTGGCGACCCTT
ACGCTCAACCGTCCCGAAAA

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKDRVEIFEVGPRDGLQNEKRDIPVADKVALVDCLSRAGFSRIEVASFVSPKWVPQMAGSAEVLAGITKAKGVRYAALTP
NMRGYEDALAARADEIAIFASASEGFSQANINASIAESIERFTPILEAARHIDLPVRGYVSCVTDCPYDGAVAPDKVAEV
ADRLFSMGCYEISLGDTVGKATPDKIAKMLIAVKDAVPVTRLAGHYHDTAGRAIANIDASLALGVRVFDAAVGGLGGCPY
APGAAGNVATEVVHEHLTRLGYDTGLDGTVLQEAAAMARHLRPEPTDA

Sequences:

>Translated_288_residues
MKDRVEIFEVGPRDGLQNEKRDIPVADKVALVDCLSRAGFSRIEVASFVSPKWVPQMAGSAEVLAGITKAKGVRYAALTP
NMRGYEDALAARADEIAIFASASEGFSQANINASIAESIERFTPILEAARHIDLPVRGYVSCVTDCPYDGAVAPDKVAEV
ADRLFSMGCYEISLGDTVGKATPDKIAKMLIAVKDAVPVTRLAGHYHDTAGRAIANIDASLALGVRVFDAAVGGLGGCPY
APGAAGNVATEVVHEHLTRLGYDTGLDGTVLQEAAAMARHLRPEPTDA
>Mature_288_residues
MKDRVEIFEVGPRDGLQNEKRDIPVADKVALVDCLSRAGFSRIEVASFVSPKWVPQMAGSAEVLAGITKAKGVRYAALTP
NMRGYEDALAARADEIAIFASASEGFSQANINASIAESIERFTPILEAARHIDLPVRGYVSCVTDCPYDGAVAPDKVAEV
ADRLFSMGCYEISLGDTVGKATPDKIAKMLIAVKDAVPVTRLAGHYHDTAGRAIANIDASLALGVRVFDAAVGGLGGCPY
APGAAGNVATEVVHEHLTRLGYDTGLDGTVLQEAAAMARHLRPEPTDA

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=274, Percent_Identity=55.1094890510949, Blast_Score=306, Evalue=1e-83,
Organism=Homo sapiens, GI109150422, Length=274, Percent_Identity=52.9197080291971, Blast_Score=306, Evalue=1e-83,
Organism=Homo sapiens, GI109150427, Length=274, Percent_Identity=52.9197080291971, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI260654708, Length=274, Percent_Identity=35.036496350365, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17510563, Length=266, Percent_Identity=46.6165413533835, Blast_Score=257, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24582381, Length=265, Percent_Identity=54.7169811320755, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 30351; Mature: 30351

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDRVEIFEVGPRDGLQNEKRDIPVADKVALVDCLSRAGFSRIEVASFVSPKWVPQMAGS
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHCCC
AEVLAGITKAKGVRYAALTPNMRGYEDALAARADEIAIFASASEGFSQANINASIAESIE
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHHH
RFTPILEAARHIDLPVRGYVSCVTDCPYDGAVAPDKVAEVADRLFSMGCYEISLGDTVGK
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
ATPDKIAKMLIAVKDAVPVTRLAGHYHDTAGRAIANIDASLALGVRVFDAAVGGLGGCPY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCC
APGAAGNVATEVVHEHLTRLGYDTGLDGTVLQEAAAMARHLRPEPTDA
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKDRVEIFEVGPRDGLQNEKRDIPVADKVALVDCLSRAGFSRIEVASFVSPKWVPQMAGS
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHCCC
AEVLAGITKAKGVRYAALTPNMRGYEDALAARADEIAIFASASEGFSQANINASIAESIE
HHHHHHHHHHCCCEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHHCCCCHHHHHHHH
RFTPILEAARHIDLPVRGYVSCVTDCPYDGAVAPDKVAEVADRLFSMGCYEISLGDTVGK
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCC
ATPDKIAKMLIAVKDAVPVTRLAGHYHDTAGRAIANIDASLALGVRVFDAAVGGLGGCPY
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHCCCCCCCC
APGAAGNVATEVVHEHLTRLGYDTGLDGTVLQEAAAMARHLRPEPTDA
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]