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The map label for this gene is puuD [H]

Identifier: 99080551

GI number: 99080551

Start: 762988

End: 763779

Strand: Reverse

Name: puuD [H]

Synonym: TM1040_0710

Alternate gene names: 99080551

Gene position: 763779-762988 (Counterclockwise)

Preceding gene: 99080552

Following gene: 99080549

Centisome position: 23.86

GC content: 61.36

Gene sequence:

>792_bases
ATGTGGAGATCAAACGTGCGCCCCAAAGTCGGCATCATCGGCAATTCCTACCTCATGAATGACCAGTACCCGACACATGC
CGGGGGCACCATGAACTCGGATGCTGTCGCCAATGTGGCGGGGTGCCTGCCTCTGTTGATCCCGACCGACCCGCGATTTG
TTTCTGTCGAGGAACTGCTCGAGAGCTTTGATGGTTTCTTGTTGACCGGCGGCCGCCCGAACGTGCACCCGCAGGAATAT
GGCGAAGACCCCACCGAGGCTCATGGAGATTTTGACCGCGCGCGCGATGCCATCACCCTGCCGCTGGTGCGGGAATGTGT
GAAACGCGGCCAGCCGTTTTTTGGGATCTGCCGAGGCTTTCAAGAGGTGAACGTCGCCATGGGCGGCACGCTCTACCCTG
AAATTCGCGACCTACCCGGACGTATGAACCACCGCATGCCCCCCGATGGCACCATCGAAGAGAAATTCGCGCTGCGCCAT
CCGGTCAAGCTGCGCGAAGGTGGCATATTCCATCAGCTTCTGGGCGCCGCGGAGGTGATGACCAACACGCTGCATGGACA
GGGGATCAAAACACCTGGTGCCCGCATCGTTGTGGAAGGCGAAGCCGAAGACGGCACACCCGAAGCCCTCTATGTCAAGG
ACGCGGCTGGATTTTCACTCGCCGTGCAATGGCACCCCGAGTATCGTGCAGGCGAGGATCCGGTGTCGCGCCCCCTCTTT
GAGGCCTTTGGCCGCGCGGTCCACAGCTGGTCAGAAGACCGGCGCCCCGCTGCCCTGTCGCTGTCGGCCTAA

Upstream 100 bases:

>100_bases
AGGTCTGTGCATAGGTGAACAGGCCAATGCGCCTCGCCTCGGGCTTGCGCAAAAGACAAGCCACATTAAGGTGATAGGGC
AACAGACCCTGAACCAAACG

Downstream 100 bases:

>100_bases
CCCACGACTTTTGCGTCATTTTGCTGCCAAATCGCCCGCGACAACGGTCAGTTTGTCGATCTTATCCGGAATCTTGGCGG
TATCGGCGGTTGAAATGTCG

Product: peptidase C26

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MWRSNVRPKVGIIGNSYLMNDQYPTHAGGTMNSDAVANVAGCLPLLIPTDPRFVSVEELLESFDGFLLTGGRPNVHPQEY
GEDPTEAHGDFDRARDAITLPLVRECVKRGQPFFGICRGFQEVNVAMGGTLYPEIRDLPGRMNHRMPPDGTIEEKFALRH
PVKLREGGIFHQLLGAAEVMTNTLHGQGIKTPGARIVVEGEAEDGTPEALYVKDAAGFSLAVQWHPEYRAGEDPVSRPLF
EAFGRAVHSWSEDRRPAALSLSA

Sequences:

>Translated_263_residues
MWRSNVRPKVGIIGNSYLMNDQYPTHAGGTMNSDAVANVAGCLPLLIPTDPRFVSVEELLESFDGFLLTGGRPNVHPQEY
GEDPTEAHGDFDRARDAITLPLVRECVKRGQPFFGICRGFQEVNVAMGGTLYPEIRDLPGRMNHRMPPDGTIEEKFALRH
PVKLREGGIFHQLLGAAEVMTNTLHGQGIKTPGARIVVEGEAEDGTPEALYVKDAAGFSLAVQWHPEYRAGEDPVSRPLF
EAFGRAVHSWSEDRRPAALSLSA
>Mature_263_residues
MWRSNVRPKVGIIGNSYLMNDQYPTHAGGTMNSDAVANVAGCLPLLIPTDPRFVSVEELLESFDGFLLTGGRPNVHPQEY
GEDPTEAHGDFDRARDAITLPLVRECVKRGQPFFGICRGFQEVNVAMGGTLYPEIRDLPGRMNHRMPPDGTIEEKFALRH
PVKLREGGIFHQLLGAAEVMTNTLHGQGIKTPGARIVVEGEAEDGTPEALYVKDAAGFSLAVQWHPEYRAGEDPVSRPLF
EAFGRAVHSWSEDRRPAALSLSA

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=255, Percent_Identity=35.2941176470588, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28871; Mature: 28871

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRSNVRPKVGIIGNSYLMNDQYPTHAGGTMNSDAVANVAGCLPLLIPTDPRFVSVEELL
CCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCEECHHHHH
ESFDGFLLTGGRPNVHPQEYGEDPTEAHGDFDRARDAITLPLVRECVKRGQPFFGICRGF
HHCCCEEEECCCCCCCHHHHCCCCCHHCCCHHHHHCCEEHHHHHHHHHCCCCHHHHHHCH
QEVNVAMGGTLYPEIRDLPGRMNHRMPPDGTIEEKFALRHPVKLREGGIFHQLLGAAEVM
HHHHHHCCCEECCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHH
TNTLHGQGIKTPGARIVVEGEAEDGTPEALYVKDAAGFSLAVQWHPEYRAGEDPVSRPLF
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHH
EAFGRAVHSWSEDRRPAALSLSA
HHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MWRSNVRPKVGIIGNSYLMNDQYPTHAGGTMNSDAVANVAGCLPLLIPTDPRFVSVEELL
CCCCCCCCCEEEEECCEEECCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCEECHHHHH
ESFDGFLLTGGRPNVHPQEYGEDPTEAHGDFDRARDAITLPLVRECVKRGQPFFGICRGF
HHCCCEEEECCCCCCCHHHHCCCCCHHCCCHHHHHCCEEHHHHHHHHHCCCCHHHHHHCH
QEVNVAMGGTLYPEIRDLPGRMNHRMPPDGTIEEKFALRHPVKLREGGIFHQLLGAAEVM
HHHHHHCCCEECCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHCCCCHHHHHHHHHHHH
TNTLHGQGIKTPGARIVVEGEAEDGTPEALYVKDAAGFSLAVQWHPEYRAGEDPVSRPLF
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCHHHHHHH
EAFGRAVHSWSEDRRPAALSLSA
HHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]