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The map label for this gene is pntA [H]

Identifier: 99080549

GI number: 99080549

Start: 761076

End: 762650

Strand: Reverse

Name: pntA [H]

Synonym: TM1040_0708

Alternate gene names: 99080549

Gene position: 762650-761076 (Counterclockwise)

Preceding gene: 99080551

Following gene: 99080548

Centisome position: 23.83

GC content: 61.46

Gene sequence:

>1575_bases
TTGAAAATAGGCACACCAAAAGAAATAGCCGAGGGCGAGTCGCGCGTTGCGATGACGCCAGCCTCGGCAAAAGATCTTCA
GAAGCTGGGGTTTGAGTGCTGCATTGAGGCAGGCGCCGGGGAAGCTGCGGGCTTTACCGATGCGGCCTACGCCGAAGCCG
GTGTTGAGGTCATCAAGACCGCCGCCGGGCTGTGGAAGGCCGCCGATATCATCGCTAAGGTGCGCGCGCCCTCCGAGACC
GAGCTGAAGCGGCTCAACAAATCCAAGACCCTGATTTCCTTCTTCAACCCGGGCGGCAATGAAGAGGGCCTCGAGCTCGC
GAAATCCAAGGGCGCGACCGTGATCGCTATGGAAATGGTGCCGCGCATCAGCCGCGCGCAAAAGATGGACGCACTGAGCT
CCATGGCCAATATCGCGGGCTACCGCGCGGTGATCGAGGCGGGCAACAACTTTGACCGGTTCTTCACCGGCCAGATCACC
GCTGCGGGCAAGGTGCCCCCGGCCAAGGTGCTGGTCGTGGGCGCAGGAGTTGCGGGCCTCGCGGCAATCGGCACCTCCAC
CTCGCTAGGCGCGGTGACCTATGCGTTTGACGTGCGCCCCGAAGTGGCCGAACAGGTCGAGTCGATGGGTGCCGAGTTTG
TCTACCTCGACTTTGAGGAAGAACAGCAGGATGGCGCGGCCACAGGCGGTTACGCGTCTGTTTCGAGCCCCGAGTTCCGA
GAGGCCCAGCTGGCCAAATTCCGCGAACTGGCGCCCGAAATGGACATCGTGATCACCACCGCGCTGATCCCCAACCGCGA
GGCGCCGAAGCTCTGGCTGGCGGATATGGTCGCGGCGATGAAGCGCGGTTCAGTGATTGTGGACCTTGCGGCCGAGCGCG
GCGGCAACGTCGAAGGCACCGTGAAGGACGAGAAAGTCGTCACCGATAACGGCGTGACCATCATCGGCTACACCGACTTC
CCAAGCCGCATGGCGAGCCAGTCCTCCAGCCTCTATGCCACCAACATCCGTCACATGATGACCGATCTCACCCCGGAAAA
GGACGGTCAGGTGGTGCATGACATGGAGGATGACGTGATCCGCGGGGCCACTGTGACCCACGCAGGCGAGATCACCTTCC
CACCGCCGCCGCCCAAGGTGCAGGCCATTGCGGCGCAAAAGAAGCCCGAAGCCCCCAAGGAGCTGACGGCCGAGGAAAAA
CGCGCCGCAGAAGTCGCCGCCTTCAAACAACAGACCAAAAATCAGGTCACATTGCTTGCGGTCGGCGCTGCGTTGCTCCT
GGCGGTGGGGCTGGTGGCCCCTGCAAGCTTCATGCAGCATTTCATCGTCTTTGTGCTCTCGGTCTTTGTGGGCTTCCAGG
TGATCTGGAACGTCTCGCACTCCCTACACACGCCGCTGATGGCGGTCACAAATGCGATCTCCTCGATCATCATTCTCGGC
GCCCTGATGCAGATCGGCTCTGGTTCCTGGTTGGTGATTTTCCTGGCCGCTCTCAGTGTCTTTATGGCCGGGATCAACAT
CTTTGGCGGCTTCCTCGTGACACGGCGCATGCTCGCCATGTTCCAGAAATCCTAA

Upstream 100 bases:

>100_bases
CAGCAAATTGCTTGCGCAATTTTGTTGCGCTGTGTATTTAATGTGTTTGCGAAACGTGCGCAAGCATTCGCAACCATCGG
TGCAGACAGGGGGATGACTG

Downstream 100 bases:

>100_bases
AGGAGGAGAGATCATGGACTTTGGATTCACGACGGCGGCCTATGTGGTCGCAGCGGTTCTCTTTATCCTTTCGCTGGGCG
GACTCTCGGGCCAGGAAAGC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKIGTPKEIAEGESRVAMTPASAKDLQKLGFECCIEAGAGEAAGFTDAAYAEAGVEVIKTAAGLWKAADIIAKVRAPSET
ELKRLNKSKTLISFFNPGGNEEGLELAKSKGATVIAMEMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFDRFFTGQIT
AAGKVPPAKVLVVGAGVAGLAAIGTSTSLGAVTYAFDVRPEVAEQVESMGAEFVYLDFEEEQQDGAATGGYASVSSPEFR
EAQLAKFRELAPEMDIVITTALIPNREAPKLWLADMVAAMKRGSVIVDLAAERGGNVEGTVKDEKVVTDNGVTIIGYTDF
PSRMASQSSSLYATNIRHMMTDLTPEKDGQVVHDMEDDVIRGATVTHAGEITFPPPPPKVQAIAAQKKPEAPKELTAEEK
RAAEVAAFKQQTKNQVTLLAVGAALLLAVGLVAPASFMQHFIVFVLSVFVGFQVIWNVSHSLHTPLMAVTNAISSIIILG
ALMQIGSGSWLVIFLAALSVFMAGINIFGGFLVTRRMLAMFQKS

Sequences:

>Translated_524_residues
MKIGTPKEIAEGESRVAMTPASAKDLQKLGFECCIEAGAGEAAGFTDAAYAEAGVEVIKTAAGLWKAADIIAKVRAPSET
ELKRLNKSKTLISFFNPGGNEEGLELAKSKGATVIAMEMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFDRFFTGQIT
AAGKVPPAKVLVVGAGVAGLAAIGTSTSLGAVTYAFDVRPEVAEQVESMGAEFVYLDFEEEQQDGAATGGYASVSSPEFR
EAQLAKFRELAPEMDIVITTALIPNREAPKLWLADMVAAMKRGSVIVDLAAERGGNVEGTVKDEKVVTDNGVTIIGYTDF
PSRMASQSSSLYATNIRHMMTDLTPEKDGQVVHDMEDDVIRGATVTHAGEITFPPPPPKVQAIAAQKKPEAPKELTAEEK
RAAEVAAFKQQTKNQVTLLAVGAALLLAVGLVAPASFMQHFIVFVLSVFVGFQVIWNVSHSLHTPLMAVTNAISSIIILG
ALMQIGSGSWLVIFLAALSVFMAGINIFGGFLVTRRMLAMFQKS
>Mature_524_residues
MKIGTPKEIAEGESRVAMTPASAKDLQKLGFECCIEAGAGEAAGFTDAAYAEAGVEVIKTAAGLWKAADIIAKVRAPSET
ELKRLNKSKTLISFFNPGGNEEGLELAKSKGATVIAMEMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFDRFFTGQIT
AAGKVPPAKVLVVGAGVAGLAAIGTSTSLGAVTYAFDVRPEVAEQVESMGAEFVYLDFEEEQQDGAATGGYASVSSPEFR
EAQLAKFRELAPEMDIVITTALIPNREAPKLWLADMVAAMKRGSVIVDLAAERGGNVEGTVKDEKVVTDNGVTIIGYTDF
PSRMASQSSSLYATNIRHMMTDLTPEKDGQVVHDMEDDVIRGATVTHAGEITFPPPPPKVQAIAAQKKPEAPKELTAEEK
RAAEVAAFKQQTKNQVTLLAVGAALLLAVGLVAPASFMQHFIVFVLSVFVGFQVIWNVSHSLHTPLMAVTNAISSIIILG
ALMQIGSGSWLVIFLAALSVFMAGINIFGGFLVTRRMLAMFQKS

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=541, Percent_Identity=45.1016635859519, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI122939153, Length=541, Percent_Identity=45.1016635859519, Blast_Score=424, Evalue=1e-118,
Organism=Escherichia coli, GI1787887, Length=524, Percent_Identity=53.2442748091603, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17550456, Length=528, Percent_Identity=46.4015151515151, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 55607; Mature: 55607

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGTPKEIAEGESRVAMTPASAKDLQKLGFECCIEAGAGEAAGFTDAAYAEAGVEVIKT
CCCCCCHHHHCCCCCEEECCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AAGLWKAADIIAKVRAPSETELKRLNKSKTLISFFNPGGNEEGLELAKSKGATVIAMEMV
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHH
PRISRAQKMDALSSMANIAGYRAVIEAGNNFDRFFTGQITAAGKVPPAKVLVVGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECCCCCCCEEEEEECCHHHH
AAIGTSTSLGAVTYAFDVRPEVAEQVESMGAEFVYLDFEEEQQDGAATGGYASVSSPEFR
HHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCCCCCHH
EAQLAKFRELAPEMDIVITTALIPNREAPKLWLADMVAAMKRGSVIVDLAAERGGNVEGT
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCE
VKDEKVVTDNGVTIIGYTDFPSRMASQSSSLYATNIRHMMTDLTPEKDGQVVHDMEDDVI
ECCCEEEECCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEECCCCHHHH
RGATVTHAGEITFPPPPPKVQAIAAQKKPEAPKELTAEEKRAAEVAAFKQQTKNQVTLLA
CCCEEECCCEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHH
VGAALLLAVGLVAPASFMQHFIVFVLSVFVGFQVIWNVSHSLHTPLMAVTNAISSIIILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALMQIGSGSWLVIFLAALSVFMAGINIFGGFLVTRRMLAMFQKS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIGTPKEIAEGESRVAMTPASAKDLQKLGFECCIEAGAGEAAGFTDAAYAEAGVEVIKT
CCCCCCHHHHCCCCCEEECCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
AAGLWKAADIIAKVRAPSETELKRLNKSKTLISFFNPGGNEEGLELAKSKGATVIAMEMV
HHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHH
PRISRAQKMDALSSMANIAGYRAVIEAGNNFDRFFTGQITAAGKVPPAKVLVVGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECCCCCCCEEEEEECCHHHH
AAIGTSTSLGAVTYAFDVRPEVAEQVESMGAEFVYLDFEEEQQDGAATGGYASVSSPEFR
HHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEEECCHHHCCCCCCCCCCCCCCCCHH
EAQLAKFRELAPEMDIVITTALIPNREAPKLWLADMVAAMKRGSVIVDLAAERGGNVEGT
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCE
VKDEKVVTDNGVTIIGYTDFPSRMASQSSSLYATNIRHMMTDLTPEKDGQVVHDMEDDVI
ECCCEEEECCCEEEEEECCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCEECCCCHHHH
RGATVTHAGEITFPPPPPKVQAIAAQKKPEAPKELTAEEKRAAEVAAFKQQTKNQVTLLA
CCCEEECCCEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHH
VGAALLLAVGLVAPASFMQHFIVFVLSVFVGFQVIWNVSHSLHTPLMAVTNAISSIIILG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALMQIGSGSWLVIFLAALSVFMAGINIFGGFLVTRRMLAMFQKS
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]